Antioxidative activity of Lenzites warnieri basidiocarps

Considering that mushrooms synthesize different kinds of compounds with antioxidative activity and that search for natural antioxidants is a topical study area, testing of unstudied species is fully justified. The aim of the study was to evaluate antioxidative capacity of Lenzites warnieri basidiocarps using different solvents. Antioxidative potential of 96% ethanolic, 70% ethanolic and methanolic extracts was evaluated by 2,2?-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) bleaching assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Additionally, total content of phenols and flavonoids in extracts was determined as galic acid equivalent (GAE) and quercetin equivalent (QE), respectively. Presented as EC50, 70% ethanolic extracts showed the highest antioxidative capacity by DPPH assay (3.08 ? 0.49 mg/mL) and 96% ethanolic extract by ABTS assay (3.08 ? 0.24 mg/mL). Methanolic extract exhibited the lowest antioxidative activity in both assays (6.02 ? 0.99 mg/mL and 4.92 ? 0.38 mg/mL, respectively). Results showed that antioxidative capacity of extracts depended on solvents and assay used, indicating that ethanolic extracts were with higher capacity in free radicals neutralization. The highest content of total phenols was detected in 70% ethanolic extract (37.45 ? 0.36 ?g GAE/mg of dried extract) while the lowest amount was noted in methanolic extract (22.73 ? 0.05 ?g GAE/mg of dried extract). Total flavonoid contents were negligible and ranged between 1.91 ? 0.10 and 2.24 ? 0.13 ?g QE/mg of dried extract. The obtained results indicate that Lenzites warnieri possess significant antioxidative capacity which is mainly correlated to phenols present in the extracts.

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EVALUATION OF ANTI-ALZHEIMER ACTIVITY OF ETHANOLIC AND METHANOLIC EXTRACTS OF POLYGONUM GLABRUM AGAINST ALUMINUM CHLORIDE-INDUCED ALZHEIMER’S IN EXPERIMENTAL RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i1.39934 ◽

2021 ◽

pp. 118-125

Author(s):

SUNEESHA Y ◽

VINAY KUMAR T

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Aluminum Chloride ◽

Methanolic Extract ◽

Ethanolic Extract ◽

Morris Water Maze Test ◽

Methanolic Extracts ◽

Maze Test ◽

Behavioral Parameters ◽

Acetylcholinesterase Enzyme

Objective: The current study aimed at the investigation of the effectiveness of ethanolic and methanolic extract of Polygonum glabrum in aluminum chloride-induced Alzheimer’s disease in experimental rats. Methods: The behavioral parameters evaluated by following methods such as Morris water maze test, radial arm maze test, and active avoidance test. Biochemical parameters were also estimated such as acetylcholine and acetylcholine esterase. Results: Polygonum glabrum extract was instituted to be neuroprotective against AlCl3-induced toxicity. Enhanced learning and memory were allied to the ingestion of extract in rats. Al overload, acetylcholinesterase enzyme hyperactivity is responsible for Alzheimer’s disease which is neutralized or reduced with treatment of extract, which might be due to the synergistic action of its active constituents. Ethanolic extract was shown slightly higher efficacy as compared to methanolic extract. Conclusion: Based on these current findings, it is suggested that lowering Aβ is an unproven strategy, and it may be time to refocus on other targets for the treatment of this disease, including pathological forms of tau.

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Phytochemical investigation and determination of phytoconstituents in flower extract of Nelumbo nucifera

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i1.2197 ◽

2019 ◽

Vol 9 (1) ◽

pp. 146-149

Author(s):

Seema Dubey ◽

Shailbala Singh Baghel

Keyword(s):

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Nelumbo Nucifera ◽

Total Phenolic ◽

Flower Extract ◽

Phytochemical Investigation ◽

Dpph Scavenging

The aim of this study seeks to investigate the presence of qualitative and quantitative analysis of phytoconstituents of the flower of the plant Nelumbo nucifera. The present study provides evidence that successive solvent extract of Nelumbo nucifera contains medicinally important bioactive compounds and this justifies the use of plant species as traditional medicine for treatment of various diseases. Maximum phenolic and flavanoid content was observed in ethanolic extract of Nelumbo nucifera. The DPPH scavenging potential of the ethanolic extracts of the herbs ranged from 33%-55%. In the present study analysis of free radical scavenging activity and total phenolic and flavonoid content showed that mainly the ethanolic extract of flower extract can be the potent source of natural antioxidants. Keywords: Phytochemical, Ethanolic extract, Antioxidant activity, Nelumbo nucifera

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Reversible Nematostatic Effect of Peganum harmala L (Nitrariaceae) on Meloidogyne javanica

Journal of AgriSearch ◽

10.21921/jas.v6i1.14917 ◽

2019 ◽

Vol 6 (1) ◽

Cited By ~ 1

Author(s):

EL HASSAN MAYAD ◽

KHADIJA BASAID ◽

JAMES NICHOLAS FURZE ◽

NIAMA HEIMEUR ◽

BTISSAM SENHAJI ◽

...

Keyword(s):

Methanolic Extract ◽

Total Content ◽

Meloidogyne Javanica ◽

Aqueous Extracts ◽

In Vitro Experiments ◽

Peganum Harmala ◽

Root Knot Nematode ◽

Second Stage ◽

Methanolic Extracts

Meloidogyne javanica is considered as the most damaging nematode of vegetables in Morocco. Eco-friendly bionematicides are urgently required for its control. In vitro experiments were carried out to assess the direct effect of bioproducts of Peganum harmala against M. javanica. The bioassay showed extracts to be nematotoxic. Aqueous extracts of P. harmala exhibited reversible nematostatic activity. The estimated ID50 of the most active product in methanolic extracts was 368ppm. HPLC-MS of the methanolic extract revealed that total content of major alkaloids of P. harmala was approximately 12.162±0.637mg/g. Harmine (8.514±0.521mg/g) is the dominant alkaloid. In conclusion, Peganum harmala has a reversible nematostatic activity on second stage juveniles of M. javanica. The effect of P. harmala is due to its possession of a high content of β-carboline alkaloids, which warrant further experimentation. Bioproducts from P. harmala should be exploited through formulations for management of the root knot nematode.

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Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis

Revista Brasileira de Pesquisa em Alimentos ◽

10.14685/rebrapa.v1i2.29 ◽

2010 ◽

Vol 1 (2) ◽

pp. 129

Author(s):

Denise Alves Vieira ◽

Pauline Sambugaro Santos ◽

Charles Windson Isidoro Haminiuk ◽

Manuel Salvador Vicente Plata-Oviedo

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Total Content ◽

Natural Antioxidants ◽

Superior Performance ◽

Good Ability ◽

Ethanolic Extracts ◽

Radical Method ◽

Gallic Acid Equivalent ◽

Potential Use

The objective of this work was to quantify the total content of phenolic compounds and the antioxidant activity of ethanolic extracts of acerola, guabiroba and ora-pro-nobis leaves. The analysis was performed by the DPPH free-radical method, aiming to find plants with potential use as natural antioxidants in foodstuff. In the extraction of phenolic compounds from leaves, acidified methanol 80% showed superior performance when compared to ethanol 80%. It was found the amounts of 1656.66, 5196.66 and 1693.33 mg GAE (gallic acid equivalent)/100 g for acerola, guabiroba and ora-pro-nobis leaves, respectively. Ethanolic extracts of guabiroba and ora-pro-nobis leaves (8.57 µg GAE/mL) presented higher antioxidant activity (92.6 and 92.76%, respectively) than BHT (10.13%), ascorbic acid (19.25%) and similar to trolox (92.79%). The extract of ora-pro-nobis presented the lowest IC50 (3.22 μg GAE/mL) showing a good ability to capture the DPPH radical. DOI: http://dx.doi.org/10.14685/rebrapa.v1i2.29

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Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-021-00151-0 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Safynaz Magdy Hanafy ◽

Yasser Mohamed Abd El-Shafea ◽

Waleed Diaeddeen Saleh ◽

Hayam Mohamed Fathy

Keyword(s):

Methanolic Extract ◽

Banana Peel ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Total Flavonoids ◽

Fragmentation Pattern ◽

Pomegranate Peel ◽

Mass Spectral Fragmentation ◽

Methanolic Extracts ◽

Ethanolic Extracts

Abstract Background The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Results The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains revealed that both pomegranate peel extracts exhibited significantly higher inhibitory effect on all tested G+ bacteria. Methanolic extract of pomegranate peel gave higher activity than the ethanolic one against G+ and G− bacteria except for S. typhimurium. Against A. flavus and A. niger, both pomegranate and orange extracts showed activity ranging between 65 and 100% more than the positive control. The ethanolic extracts of all tested peels showed a considerable capacity of antioxidant compounds compared to the methanolic extracts. The highest antioxidant capacity was found for ethanolic and methanolic extracts of pomegranate, 66.870 and 56.262 mg/ml, respectively. Generally, the concentration of total phenolic compounds was higher than that of total flavonoids followed by tannins. The highest readings of all tested constituents were reported for pomegranate extracts followed by orange and then banana. The total phenolic content, total flavonoids, and tannins were proportional to antioxidant values. GC-MS of pomegranate peel extracts identified 23 compounds in the methanolic extract versus 31 compounds in the ethanolic one. These components were identified based on their retention times and mass spectral fragmentation pattern. 5-hydroxymethylfufural (HMF) represented the major component in both methanolic and ethanolic extracts with peak area percentage of 65.78% and 48.43%, respectively. Conclusions The results showed negative effect of methanolic and ethanolic extracts of pomegranate on G+ and G− bacteria and two fungal pathogenic strains. The phytochemical analysis regarded these results to the high content of phenols, flavonoids, and tannins. GC-MS chromatogram identified many compounds known to be effective as antioxidants and antibacterial and antifungal agents. These indications show that pomegranate peel may be a superior natural food-preserver, but further studies about the suitable formulation, dosage, and possible side-effects are still needed.

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In VitroAnti-CandidaActivity ofZataria multifloraBoiss

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nel099 ◽

2007 ◽

Vol 4 (3) ◽

pp. 351-353 ◽

Cited By ~ 42

Author(s):

Ali Zarei Mahmoudabadi ◽

Muhammad Ali Dabbagh ◽

Zahra Fouladi

Keyword(s):

Medicinal Plants ◽

Minimal Inhibitory Concentration ◽

Candida Parapsilosis ◽

Inhibitory Concentration ◽

Methanolic Extract ◽

Ethanolic Extract ◽

Zataria Multiflora ◽

Aerial Parts ◽

Ethanolic Extracts

Zataria multifloraBoiss known as Avishan Shirazi (in Iran) is one of the valuable Iranian medicinal plants. The aim of study was to evaluate anti-Candidaactivity ofZ. multifloraagainst different species ofCandida in vitro. Anti-Candidaactivity of the aqueous, ethanolic and methanolic maceration extract of the aerial parts ofZ. multiflora Boisswas studiedin vitro. Anti-Candidaactivity againstCandidaspecies was done using serial dilutions of extracts in Sabouraud's dextrose agar. Minimal inhibitory concentration (MIC) of the methanolic and ethanolic extracts was 70.7 and 127 mg l−1, respectively. Aqueous extract showed no remarkable activity againstCandidaspecies. We conclude that methanolic extract of the aerial parts ofZ. multifloraBoiss has more anti-Candidaeffect at 70.7 mg l−1compared to ethanolic extract 127 mg l−1. In addition, the isolates ofCandida parapsilosiswere more susceptible to methanolic extract than other tested species.

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Screening of Microalgae for Antioxidant Activities, Carotenoids and Phenolic Contents

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.625.156 ◽

2014 ◽

Vol 625 ◽

pp. 156-159 ◽

Cited By ~ 2

Author(s):

Hamdy Elsayed Ahmed Ali ◽

Sanaa Mahmoud Metwally Shanab ◽

Mervat Aly Mohamed Abo-State ◽

Emad Ahmed Ahmed Shalaby ◽

Usama Eldmerdash ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Scenedesmus Obliquus ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Carotenoid Content ◽

Total Phenolic ◽

Microalgal Biomass ◽

Phenolic Contents ◽

Dpph Scavenging

Ten different microalgal species were screened for their antioxidant activity using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, both total phenolic and carotenoid contents were determined. The ethanolic extract ofOscillatoriasp. showed the highest antioxidant activity (69.1 %), while the greenChlorellasp. showed higher phenolic content (39.1 mg GAE g-1dry wt.) andScenedesmus obliquusrecorded higher carotenoid content (3.73 mg L-1), than cyanobacterial species Microalgal biomass plays a great role as a potential source of natural antioxidants, not only due to the carotenoids but also the phenolic compounds.

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Phytochemical characterization, in-vitro antioxidant and an bacterial activities of somes extract of Ammodaucus leucotrichus Coss. & Dur. from Bechar, Algeria

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.9(5).p166-172 ◽

2020 ◽

Vol 9 (5) ◽

pp. 166-172

Author(s):

Achraf Khaldi ◽

Abdellah Moussaoui ◽

Boumedien Meddah

Keyword(s):

Ascorbic Acid ◽

Methanolic Extract ◽

Bacterial Activity ◽

Diffusion Method ◽

Dilution Method ◽

Agar Dilution Method ◽

Total Phenol Content ◽

Methanolic Extracts ◽

Dpph Scavenging

The extracts of plants have raised benefit as sources of natural products. They have been examined for their potential uses as alternative solution for the treatment of many infectious diseases. To this end, the present study examines the phytochemical screening, an oxidant and an bacterial properties of crude aqueous, ethyl acetate and methanolic extracts of Ammodaucus leucotrichus Coss. & Dur. seeds growing spontaneously in the Wilaya of Bechar. Phytochemical invesyigation was carried out to determine the possible bioactive components in the extracts of plant . The total phenol content and avonoids were also determined for all extracts. The an oxidant ac vi es were es mated by DPPH scavenging method compared to the ascorbic acid an oxidant. The methanolic extract showed a good scavenging ac vity comparable to ascorbic acid, with an IC50 value of 0.10±0.14 mg/l. An bacterial activity was tested against seven pathogenic strains by using Disc diffusion method and agar dilution method. Among the extracts tested, methanolic extract showed significant an bacterial activity against bacteria with MIC between 0.95 to 6.02 mg/ml. These results are promising and provide scientific that this species can be used as good an oxidant and an bacterial agents.

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Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants

Hemijska industrija ◽

10.2298/hemind140830013j ◽

2016 ◽

Vol 70 (1) ◽

pp. 99-106 ◽

Cited By ~ 1

Author(s):

Violeta Jakovljevic ◽

Jasmina Milicevic ◽

Gorica Ðelic ◽

Miroslav Vrvic

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Reducing Power ◽

Ethanolic Extract ◽

Natural Antioxidants ◽

Total Phenolic ◽

Scavenging Activity ◽

Flavonoid Content ◽

Dpph Scavenging

The antioxidant activity, total phenolic and flavonoid content of ethanolic, acetone and ethyl acetate extracts of Ruscus hypoglossum L. and Ruscus aculeatus L. (aerial parts) from Serbia were investigated in this paper. The best total antioxidant capacity (23.329 ?g AA g-1) and the highest DPPH scavenging activity (IC50 = 182.54 ?g mL-1) were found in acetone and ethyl acetate extract of R. aculeatus L. Ethanolic extract of R. hypoglossum L. showed the highest ABTS radical cation scavenging activity (IC50 = 3.04 ?g mL-1) as well as reducing power (IC50 = 0.143 ?g mL-1). The best inhibitory activity against lipid peroxidation (IC50 =651 ?g mL-1) and the best ferrous ion chelating ability (IC50 = 110 ?g mL-1) were found in acetone and ethyl acetate extract of R. hypoglossum L. The highest total phenolic (8.569 mg GAE g-1) and flavonoid content (0.136 mg RU g-1) were found in ethanolic and acetone extract of R. hypoglossum L. and R. aculeatus L, respectively.

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Phytochemical screening of Pulsatilla species and investigation of their biological activities

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.3613 ◽

2019 ◽

Vol 88 (1) ◽

Author(s):

Grażyna Łaska ◽

Aneta Sienkiewicz ◽

Marcin Stocki ◽

Jordan K. Zjawiony ◽

Vimal Sharma ◽

...

Keyword(s):

Chromatographic Analysis ◽

Inhibitory Concentration ◽

Methanolic Extract ◽

Biological Activities ◽

Ethanolic Extract ◽

Biologically Active ◽

Phytochemical Screening ◽

Methanolic Extracts ◽

Mammalian Cell Lines ◽

Pulsatilla Patens

The present study aimed to identify biologically active secondary metabolites from the rare plant species, Pulsatilla patens subsp. patens and the cultivated P. vulgaris subsp. vulgaris. Chromatographic fractionation of the ethanolic extract of the roots of P. patens subsp. patens resulted in the isolation of two oleanane-type glycosides identified as hederagenin 3-O-β-d-glucopyranoside (2.7 mg) and hederagenin 3-O-β-d-galactopyranosyl-(1→2)-β-d-glucopyranoside (3.3 mg, patensin). HPLC analysis of the methanolic extract of the crude root of P. patens subsp. patens and P. vulgaris subsp. vulgaris revealed the presence of Pulsatilla saponin D (hederagenin 3-O-α-l-rhamnopyranosyl(1→2)-[β-d-glucopyranosyl(1→4)]-α-l-arabinopyranoside). Chromatographic analysis using GC-MS of the silylated methanolic extracts from the leaves and roots of these species identified the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids. The extracts from Pulsatilla species were tested for their antifungal, antimicrobial, and antimalarial activities, and cytotoxicity to mammalian cell lines. Both P. patens subsp. patens and P. vulgaris subsp. vulgaris were active against the fungus Candida glabrata with the half-maximal inhibitory concentration (IC50) values of 9.37 µg/mL and 11 µg/mL, respectively. The IC50 values for cytotoxicity evaluation were in the range of 32–38 μg/mL for P. patens subsp. patens and 35–57 μg/mL for P. vulgaris subsp. vulgaris for each cell line, indicating general cytotoxic activity throughout the panel of evaluated cancer and noncancer cells.

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