scholarly journals Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Safynaz Magdy Hanafy ◽  
Yasser Mohamed Abd El-Shafea ◽  
Waleed Diaeddeen Saleh ◽  
Hayam Mohamed Fathy
Keyword(s):  
Methanolic Extract ◽  
Banana Peel ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Flavonoids ◽  
Fragmentation Pattern ◽  
Pomegranate Peel ◽  
Mass Spectral Fragmentation ◽  
Methanolic Extracts ◽  
Ethanolic Extracts

Abstract Background The use of natural preservatives became of great interest; good examples of these natural preservation agents are plant peels. The use of plant peels has dual benefits; first is their antimicrobial activity against food-borne pathogens, while the second is minimizing agro-industrial wastes. Results The evaluation of the antimicrobial potential of both methanolic and ethanolic extracts of three fruit peels (orange, pomegranate, and banana), against 4 Gram-positive (G+), 3 Gram-negative bacteria (G−), and 2 fungal strains revealed that both pomegranate peel extracts exhibited significantly higher inhibitory effect on all tested G+ bacteria. Methanolic extract of pomegranate peel gave higher activity than the ethanolic one against G+ and G− bacteria except for S. typhimurium. Against A. flavus and A. niger, both pomegranate and orange extracts showed activity ranging between 65 and 100% more than the positive control. The ethanolic extracts of all tested peels showed a considerable capacity of antioxidant compounds compared to the methanolic extracts. The highest antioxidant capacity was found for ethanolic and methanolic extracts of pomegranate, 66.870 and 56.262 mg/ml, respectively. Generally, the concentration of total phenolic compounds was higher than that of total flavonoids followed by tannins. The highest readings of all tested constituents were reported for pomegranate extracts followed by orange and then banana. The total phenolic content, total flavonoids, and tannins were proportional to antioxidant values. GC-MS of pomegranate peel extracts identified 23 compounds in the methanolic extract versus 31 compounds in the ethanolic one. These components were identified based on their retention times and mass spectral fragmentation pattern. 5-hydroxymethylfufural (HMF) represented the major component in both methanolic and ethanolic extracts with peak area percentage of 65.78% and 48.43%, respectively. Conclusions The results showed negative effect of methanolic and ethanolic extracts of pomegranate on G+ and G− bacteria and two fungal pathogenic strains. The phytochemical analysis regarded these results to the high content of phenols, flavonoids, and tannins. GC-MS chromatogram identified many compounds known to be effective as antioxidants and antibacterial and antifungal agents. These indications show that pomegranate peel may be a superior natural food-preserver, but further studies about the suitable formulation, dosage, and possible side-effects are still needed.

scholarly journals Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits

2020 ◽  
Vol 8 (1) ◽  
pp. 29-35
Author(s):  
Messaoudi Mohammed ◽  
Merah Maroua
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Anthocyanin ◽  
Methanolic Extracts ◽  
Almost All

Since antiquity, phenolic compounds produced by plants were known as free radical scavengers and as powerful antioxidants. Huge interest has been made by researchers to the traditional uses of medicinal plants against illnesses related to oxidative stress. This study measures the correlation that can be existed between the antioxidant capacity and phytochemical s levels of four varieties of Ficus carica fruits, figs flowers or "Bakor" as called locally in Algeria. Therefore, extracts were assessed for determining their antioxidative potentials using both test of total antioxidant capacity and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test followed by quantitative phytochemical analysis to estimate the total flavonoid level (TFL), the total phenolic level (TPL), the total anthocyanin level (TAL) and the condensed tannins level of plants methanolic extracts. A positive correlation was observed between phenolics content and the antioxidant capacity of figs flowers methanol extracts. The methanolic extract of Bechar (MeOH Var.2) chelated 87,9± 1,23 % of the DPPH free radical with IC50 value equal to 0,185 mg/g DW. A high antioxidant ability of almost all extracts is, probably, related to the appreciable rates of flavonoids, phenolics and tannins showed by those fig extracts. The highest value of phenolics level was detected among the variety 1 methanolic extract of Bechar (MeOH Var.1) of 10,4 mg GAE/g DW.

scholarly journals Phytochemical Analysis, Antioxidant and Anticancer Potential of Sideritis niveotomentosa: Endemic Wild Species of Turkey

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2420
Author(s):  
Ela Nur Şimşek Şimşek Sezer ◽  
Tuna Uysal
Keyword(s):  
Cell Lines ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Expression Levels ◽  
Phenolic Contents ◽  
Scavenging Effect ◽  
Methanolic Extracts ◽  
Total Antioxidant

Sideritis niveotomentosa Hub. -Mor. is a local endemic species belonging to the Lamiaceae family. In this study, GC/MS analysis, total antioxidant capacity and anticancer effects of different extracts obtained from S. niveotomentosa were investigated comparatively. Total phenolic contents of extracts were determined by the Folin–Ciocalteu method, total flavonoid contents by aluminum chloride method, and also the free radical scavenging activities of the extracts by DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay. The cytotoxic effect of the extracts was studied via MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay on DLD1, HL60 and ARH77 cell lines. Pro-apoptotic gene expression levels were also tested in the most sensitive cell line ARH77 by Real-Time PCR. The expression levels of 4 pro-apoptotic genes, APAF, BAX, CASP3, and HRK were found to be upregulated in ARH77 cells that were treated extracts. Results showed that methanolic extracts contain more phenolic content than acetone extracts, consistent with DPPH results. As a result, Sideritis niveotomentosa extracts, especially methanolic extracts, are rich in phenolic content and have a strong radical scavenging effect. In addition, the extracts showed selective effects on cell lines. This study is pioneering in terms of future studies, and the findings provide hope for future experimentation.

scholarly journals Antioxidative activity of Lenzites warnieri basidiocarps

2017 ◽  
pp. 163-171
Author(s):  
Aleksandar Knezevic ◽  
Ivan Milovanovic ◽  
Jelena Vukojevic
Keyword(s):  
Antioxidative Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Total Content ◽  
Natural Antioxidants ◽  
Antioxidative Capacity ◽  
Methanolic Extracts ◽  
Ethanolic Extracts ◽  
Dpph Scavenging ◽  
Dried Extract

Considering that mushrooms synthesize different kinds of compounds with antioxidative activity and that search for natural antioxidants is a topical study area, testing of unstudied species is fully justified. The aim of the study was to evaluate antioxidative capacity of Lenzites warnieri basidiocarps using different solvents. Antioxidative potential of 96% ethanolic, 70% ethanolic and methanolic extracts was evaluated by 2,2?-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) bleaching assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. Additionally, total content of phenols and flavonoids in extracts was determined as galic acid equivalent (GAE) and quercetin equivalent (QE), respectively. Presented as EC50, 70% ethanolic extracts showed the highest antioxidative capacity by DPPH assay (3.08 ? 0.49 mg/mL) and 96% ethanolic extract by ABTS assay (3.08 ? 0.24 mg/mL). Methanolic extract exhibited the lowest antioxidative activity in both assays (6.02 ? 0.99 mg/mL and 4.92 ? 0.38 mg/mL, respectively). Results showed that antioxidative capacity of extracts depended on solvents and assay used, indicating that ethanolic extracts were with higher capacity in free radicals neutralization. The highest content of total phenols was detected in 70% ethanolic extract (37.45 ? 0.36 ?g GAE/mg of dried extract) while the lowest amount was noted in methanolic extract (22.73 ? 0.05 ?g GAE/mg of dried extract). Total flavonoid contents were negligible and ranged between 1.91 ? 0.10 and 2.24 ? 0.13 ?g QE/mg of dried extract. The obtained results indicate that Lenzites warnieri possess significant antioxidative capacity which is mainly correlated to phenols present in the extracts.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT PROPERTIES OF LEAF EXTRACTS OF CARICA PAPAYA

2020 ◽  
pp. 58-62
Author(s):  
NANDINI G ◽  
GOPENATH TS ◽  
NAGALAMBIKA PRASAD ◽  
MURUGESAN KARTHIKEYAN ◽  
ASHOK GNANASEKARAN ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Isolation And Purification ◽  
Chain Reactions ◽  
Methanolic Extracts

Objective: The objective of the present study aimed at investigating the phytochemical and antioxidant properties of Carica papaya leaf extracts. Methods: As phytochemicals are biologically active compounds and a powerful group of plant chemicals, believed to stimulate the immune system along with antioxidants, the molecules which hinder oxidation of other molecules by the process of inhibiting or by generating the oxidizing chain reactions and preventing diseases. The total phenolic content (TPC) was determined by Folin-Ciocalteu method and total flavonoid contents (TFC) were determined aluminum chloride method and antioxidant by 2,2,1-diphenyl-1-picrylhydrazyl method. Results: The results of phytochemical screening revealed the presence of bioactive compounds such as alkaloid, carbohydrates, and amino acids and TPC and TFC varied among the different solvent extracts, in which methanolic extracts showed highest amount of phytochemicals and TPC and TFC and antioxidants compared to other solvents. Conclusion: The isolation and purification of specific bioactive compound may account as natural and promising medicines in exploration of new drug.

scholarly journals Evaluation of antioxidant and antimicrobial activities of chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran

2020 ◽  
Author(s):  
Somayeh Ghahari ◽  
Zahra Hadadi ◽  
Ghorban Ali Nematzadeh
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Guaiacol Peroxidase ◽  
Phytochemical Analysis ◽  
Methanolic Extracts

Abstract Background: as possible sources of natural bioactive molecules, the plant essential oils and extracts have been used globally in new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease.Methods: In this research, the antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea), through disc diffusion method. Furthermore, the essential oils of plants with the highest antibacterial activity were analyzed utilizing GC/MS. Moreover, the tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among the tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against the tested bacteria. Besides, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic and flavonoid compositions in J. conglomeratus and P. harmala, respectively.Conclusion: The results indicated that these plants could act as a promising antimicrobial agent, due to their short killing time.

scholarly journals FUNCTIONAL POTENTIAL OF UNCONVENTIONAL PLANT SOURCES

2021 ◽  
pp. 53-59
Author(s):  
SANCHITA CHANDRAKANT SANGLE ◽  
NIKITA MANOHARAN ◽  
KIRTI LIMGAOKAR ◽  
ANUKRITI NIGAM
Keyword(s):  
Cocos Nucifera ◽  
Reducing Power ◽  
Punica Granatum ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Therapeutic Effects ◽  
Methanolic Extracts

Objective: Plant phenols are extensively studied, thanks to their many prophylactic (anti-tumoural, ulcer, inflammatory) and therapeutic effects. Their many antioxidants may protect against diabetes, cancer, autoimmune diseases. Our objective was to evaluate antioxidant and antimicrobial activities of our samples: Cocos nucifera (shell), Punica granatum (peel), Citrus limonum (rind), and Ocimum sanctum (stem); in conjugation with phytochemical analysis. Methods: The hydro-methanolic extracts of the selected plant parts were assessed for polyphenols. Antioxidant (ferric reducing power assay) and antibacterial (Kirby Bauer disc diffusion) assays were run for varying concentrations against-Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi. Results: The results showed that the samples all contained polyphenols with high total phenolic activity in pomegranate and high antibacterial activity in coconut. A more comprehensive study could lead to their emphatic incorporation into mainstream medicine and pharmaceuticals–furnishing natural alternatives to their chemical counterparts. Conclusion: The present study concludes that hydro-methanolic extracts of the samples contain phytochemicals in high concentrations, conferring upon them promising antioxidant and antimicrobial activity.

scholarly journals Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Sabine Adeline Fanta Yadang ◽  
Germain Taiwe Sotoing ◽  
Katoucha Sidoine Ngatcha Zouakeu ◽  
Muhammad Ahsan Khan ◽  
Gabriel Agbor Agbor ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Hplc Analysis ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Methanolic Extracts

Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. edulis (HEC, MEC, AEC, and PC). An HPLC analysis determined the different compounds present in the extracts. High concentration of total phenolic content was observed in aqueous and methanolic extracts more than in the hydroethanolic extract though not significantly different. Flavonoids were higher in the hydroethanolic and methanolic extracts, respectively, with 14.84 mg RE/g extract and 12.02 mg RE/g extract. Tannins were also found in large amounts in the same two extracts with 26.76 mg TAE/g extract and 34.67 mg TEE/g extract. The percentage radical scavenging activity DPPH ranged between 58.63% and 94.67% for aqueous extract and for ABTS between 51.39% and 94.12% for the methanolic extract. The highest FRAP was obtained in the methanolic extract (6.73 g AAE/100 g extract). HPLC analysis revealed the presence of quercetin, rutin, and gallic acid in the different extracts. C. edulis represents a potential source of bioactive components with antioxidant capacity.

Total Phenolic Content, Anti-inflammatory, Analgesic, and Antipyretic Activities of Some Extracts of Inula viscosa (L.) from Algeria

2019 ◽  
Vol 18 (2) ◽  
pp. 81-91
Author(s):  
C. Ouahchia ◽  
F. Hamaidi-Chergui ◽  
H.-S. Cherif ◽  
R. Hemma ◽  
I. Negab ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Paw Edema ◽  
Phenol Content ◽  
Spectrophotometric Methods ◽  
Methanolic Extracts ◽  
Long Time ◽  
Therapeutic Properties ◽  
Anti Inflammatory

Inula viscosa (L.) is a medicinal plant that has been used for a long time for its many therapeutic properties, especially to treat pain and inflammation. The aim of this study was to evaluate the anti-inflammatory, analgesic, and antipyretic activities of methanolic extracts and decoctions of Inula viscosa leaves and flowers. The phenol content of the extracts was determined by spectrophotometric methods. The extracts were administered orally (400, 600, and 800 mg/kg) to mice and/or rats in the study of antiinflammatory (carrageenan-induced paw edema test), analgesic (acetic acid-induced writhes test), and antipyretic (Brewer’s yeast-induced pyrexia method) activities. The highest phenolic content was found in leaf methanolic extract. Methanolic extracts and decoctions of leaves and flowers showed significant decrease in mice paw edema after 4 h (P < 0.01; P < 0.001), and an appreciable analgesic effect at the tested doses with a maximum of writhing inhibition observed with leaf methanolic extract at the dose of 800 mg/kg (93.39%; P < 0.001). The rectal temperature of the rats decreased significantly (P < 0.01; P < 0.001) after 4 h in groups treated with leaves and flowers extracts at the doses of 600 and 800 mg/kg. The obtained results confirm the traditional uses of Inula viscosa.

Evaluation of antioxidant and antimicrobial activities of chloroformic and methanolic extracts of 6 important medicinal plants collected from Northern of Iran

2019 ◽  
Author(s):  
Somayeh Ghahari ◽  
Zahra Hadadi ◽  
Ghorban Ali Nematzadeh
Keyword(s):  
Antibacterial Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Melia Azedarach ◽  
Total Phenolic ◽  
Guaiacol Peroxidase ◽  
Phytochemical Analysis ◽  
Methanolic Extracts

Abstract Background: The plant essential oils and extracts are possible sources of natural bioactive molecules and have been selected globally for new antimicrobial compounds, food preservatives, and alternatives to treat infectious disease.Methods: In this research, antimicrobial activities of chloroformic and methanolic extracts of Sophora flavescens, Rhaponticum repens, Alhagi maurorum, Melia azedarach, Peganum harmala, and Juncus conglomeratus were evaluated against 8 bacteria (S. aureus, B. subtilis, R. toxicus, P. aeruginosa, E. coli, P. syringae, X. campestris, P. viridiflava) and 3 fungi (Pyricularia oryzae, Fusarium oxysporum and Botrytis cinerea) through disc diffusion method. Furthermore, the essential oil of plants with the highest antibacterial activity was analyzed utilizing GC/MS. Moreover, tested plants were exposed to screening for possible antioxidant effect utilizing DPPH test, guaiacol peroxidas, and catalase enzymes. Besides, the amount of total phenol and flavonoid of these plants was measured. Results: Among tested plants, methanolic and chloroformic extracts of P. harmala fruits showed the highest antibacterial activity against tested bacteria. Also, the investigation of free radical scavenging effects of the tested plants indicated the highest DPPH, protein, guaiacol peroxidase, and catalase in P. harmala, M. azedarach, J. conglomeratus fruits, and J. conglomeratus fruits, respectively. In addition, the phytochemical analysis demonstrated the greatest amounts of total phenolic, and flavonoid compositions in J. conglomeratus and P. harmala. Conclusion: The results indicated that these plants can act as a promising antimicrobial agent due to its short killing time.

scholarly journals Photoprotective Activity of Buddleja cordata Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 266
Author(s):  
Milton Abraham Gómez-Hernández ◽  
Miriam V. Flores-Merino ◽  
Jesús Enrique Sánchez-Flores ◽  
Cristina Burrola-Aguilar ◽  
Carmen Zepeda-Gómez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Methanolic Extract ◽  
Uv Light ◽  
Biological Activities ◽  
Positive Control ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Buddleja Cordata ◽  
High Concentrations

The research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of Buddleja cordata has potential photoprotective effects as these cells produce phenolic secondary metabolites (SMs). These metabolites are attributed with biological activities capable of counteracting the harmful effects caused by UVR on skin. In the present work, the methanolic extract (310–2500 µg/mL) of B. cordata cell culture showed a photoprotective effect on UVB-irradiated 3T3-Swiss albino fibroblasts with a significant increase in cell viability. The greatest photoprotective effect (75%) of the extract was observed at 2500 µg/mL, which was statistically comparable with that of 250 µg/mL verbascoside, used as positive control. In addition, concentrations of the extract higher than 2500 µg/mL resulted in decreased cell viability (≤83%) after 24 h of exposure. Phytochemical analysis of the extract allowed us to determine that it was characterized by high concentrations of total phenol and total phenolic acid contents (138 ± 4.7 mg gallic acid equivalents and 44.01 ± 1.33 mg verbascoside equivalents per gram of extract, respectively) as well as absorption of UV light (first and second bands peaking at 294 and 330 nm, respectively). Some phenylethanoid glycosides were identified from the extract.

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