scholarly journals POLIETILENA GLIKOL (PEG) DALAM MEDIA IN VITRO MENYEBABKAN KONDISI CEKAMAN YANG MENGHAMBAT TUNAS KACANG TANAH (Arachis hypogaea L.)

2005 ◽  
Vol 11 (1) ◽  
pp. 39-48
Author(s):  
Enni Suwarsi Rahayu ◽  
Edi Guhardja ◽  
Satriyas Ilyas ◽  
Sudarsono Sudarsono
Keyword(s):  
Drought Stress ◽  
Tissue Damage ◽  
Growth And Development ◽  
Total Content ◽  
Proline Content ◽  
Alternative Methods ◽  
Leaf Number ◽  
Peg 6000 ◽  
Peg Stress

The objectives of this experiment were to determine response of epycotyl of nine peanut cultivars against polyethylene glycol (PEG 6000) induced stress under in vitro conditions, effective concentration of PEG to inhibit growth and development of epycotyl, evaluate tolerance of the cultivars against PEG stress, and evaluate changes in total proline content due to PEG stress. Growing epycotyls from peanut seeds (TDK) or from embryo axis (TTK) were planted on liquid MS-0 medium containing PEG 6000 (0, 5, 10, 15, and 20 percent). Growth, development, and the tissue damage score of the epycotyl were observed after six weeks. Total content of proline were analyzed for stressed and non stressed epycotyl to determine effect of PEG stress on proline accumulation. Results of the experiment indicated that addition of PEG 6000 in to MS-0 medium inhibited growth and development of peanut epycotyls and increased its total proline content. Addition of PEG 6000 might be used to simulate drought stress under in vitro condition. PEG at 15% concentration was effective for inhibiting growth and development of epycotyl explant. The response of peanut epycotyls against medium containing 15% PEG 6000 might be used as alternative methods for screening peanut tolerance against drought stress. The TDK and TTK might be used as explant, while increased in shoot length (TTK), in leaf number (TDK and TTK), in milted leaf number (TDK and TTK), in root number (TDK) and score of tissue damage (TDK) might be used as criteria for drought tolerance.

scholarly journals Effect of Drought-Stress Conditions in Chlorophyll Content of Dendrobium sp. Planlets

2019 ◽  
Vol 6 (1) ◽  
pp. 20-26
Author(s):  
Fesya Salma Putri ◽  
Endang Nurcahyani ◽  
Yulianty Yulianty ◽  
Bambang Irawan
Keyword(s):  
Drought Stress ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Total Content ◽  
Stress Conditions ◽  
Design Factor ◽  
Peg 6000 ◽  
Low Humidity

Dendrobium sp. was a popular ornamental plant and has high economic value. One of many difficulties in the growth of orchids in Indonesia is low humidity and lack of water availability. Regulatory Substance Growth in the form of an atonic solution can increase the growth of orchid plants. The purpose of this study was to determine the effect of atonic solutions on orchid chlorophyll content in drought stress conditions. This study used a 3x3 factorial design. Factor A is the atonic solution with 3 levels of concentration: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v and 25% b/v. The parameters tested were chlorophyll a, b and total Dendrobium orchid plantlets. The results showed that atonic solution and PEG 6000 significantly affected the chlorophyll a, b and total content of chlorophyll. The higher concentration of the atonic solution, the more content of chlorophyll a, b and total plantlets of decreased in drought stress conditions in vitro.

scholarly journals Stomata Index of Cattleya sp. Lindl., Planlet in Drought-Stress Conditions

2019 ◽  
Vol 6 (1) ◽  
pp. 15-19
Author(s):  
Tara Sesafia Paletri ◽  
Endang Nurcahyani ◽  
Yulianty Yulianty ◽  
Rochmah Agustrina
Keyword(s):  
Drought Stress ◽  
Ethylene Glycol ◽  
Water Availability ◽  
Stress Conditions ◽  
Poly Ethylene Glycol ◽  
Factor B ◽  
Peg 6000 ◽  
Poly Ethylene ◽  
Concentration Levels

Cattleya was one of the most popular orchids by Indonesian. Constraints in cultivating Cattleya included the lack of water availability in an area, causing drought stress. Stress in drought in vitro can be simulated by adding Poly Ethylene Glycol 6000 and growth regulators in atonic form. The purpose was to determine the effect of atonic solution and PEG 6000 on the stomata index toward in vitro drought stress conditions. This study was carried out in a 3 x 3 factorial experiment. Factor A was an atonic solution with 3 concentration levels: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v, 25% b/v. The results showed that the higher the concentration of PEG 6000 and atonic concentrations caused a decrease in the stomata index of Cattleya orchid plantlets under drought stress conditions in vitro.

scholarly journals Growth response of diploid and tetraploid taro (Colocasia esculenta (L.) Schott) shoot culture to drought stress using polyethylene glycol

2021 ◽  
Vol 913 (1) ◽  
pp. 012016
Author(s):  
A Wulansari ◽  
A Purwito ◽  
D Sukma ◽  
TM Ermayanti
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Growth Response ◽  
Shoot Culture ◽  
Proline Content ◽  
Plant Tolerance ◽  
Two Factors ◽  
Number Of Leaves ◽  
Mortality Percentage

Abstract Taro genetic improvement through polyploidy induction is expected to be tolerant to abiotic stress. Several studies have shown that polyploidy plants have a higher adaptability to dry environments. In vitro selection technique for evaluation of plant tolerance to drought stress can be done by applying polyethylene glycol (PEG) as a selection agent. The aim of the research was to investigate the growth response of diploid and tetraploid taro shoot culture to drought stress using PEG. The experiment was conducted using a completely randomized design with two factors. The first factor was the concentration of PEG at 0, 5, 10, 15 and 20%. The second factor was Bentul taro clones which were 1 diploid clone and 2 tetraploid clones (clones 4.6.3 and 5.4.4). PEG was added to liquid MS medium containing 2 mg/l BAP. Observations of growth variables were carried out every week until the six weeks of culture. Fresh and dry weights, mortality percentage and proline content were determined at six weeks of culture. The results showed that the addition of PEG in liquid medium significantly affected the number of leaves, petiole length, number of roots, fresh and dry weights, as well as shoot mortality percentage. Differences in clones significantly affected the number of leaves, fresh and dry weights. The two factors tested (PEG and clones), gave an interaction on the number of leaves, fresh and dry weights. The proline content in all diploid and tetraploid clones showed an increase with increasing PEG concentration. The LC50 value in diploid clones was 9.82%, in tetraploid clones 4.6.3 and 5.4.4 were 14.14 and 15.45%, respectively. The results showed that PEG at 10% and 15% could be used for in vitro selection to drought stress both for diploid and tetraploid taro.

scholarly journals Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress

2020 ◽  
Vol 88 (2) ◽  
Author(s):  
. TURHADI ◽  
Hayati MINARSIH ◽  
Imron RIYADI ◽  
. PRIYONO ◽  
Asmini BUDIANI
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Proline Content ◽  
Limiting Factors ◽  
Total Chlorophyll ◽  
Peg 6000 ◽  
P5cs Gene ◽  
Transgenic Plantlets

Drought is one of the limiting factors in crop cultivation, such as in oil palm (Elaeis guineensis Jacq.). The transgenic approaches are expected to increase plant tolerance to drought stress and minimize low productivity when drought occurs. Proline is an osmoprotectant compound in plants which its biosynthesis involved the P5CS gene. The objective of this study was to evaluate the tolerance level of P5CS-transgenic oil palm to drought stress induced by polyethylene glycol 6000 (PEG-6000). In this present study, the transgenic and non-transgenic oil palms were treated by  0, 2, and 4% PEG-6000 under in vitro conditions. The experiment was arranged as a factorial completely randomized design with three replications. The drought level score, total chlorophyll content, carotenoids, and proline content, as well as P5CS gene expression in leaf tissues were observed at 7 and 14 days after stress treatments. The result showed that transgenic plantlets had a lower drought level score than those of non-transgenic lines. A concentration of 4% PEG-6000 treatment reduced the total chlorophyll and carotenoids contents than that of 2% concentration in non-transgenic plantlets at 7 and 14 day after treatments (DAT). In addition, proline content and P5CS gene expression level in transgenic had been significantly increased during stress treatment. Based on these results, it can be concluded that the P5CS transgene increased the drought stress tolerance of oil palm.

Seed Germination and Physiological Characteristics of Amaranthus mangostanus L. under Drought Stress

2011 ◽  
Vol 183-185 ◽  
pp. 1071-1074
Author(s):  
Yong Dong Sun ◽  
Xiao Hua Du ◽  
Wen Jie Zhang ◽  
Li Sun ◽  
Ran Li
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Proline Content ◽  
Shoot Length ◽  
Physiological Characteristics ◽  
Sod Activity ◽  
Peg 6000 ◽  
Mda Content ◽  
Effects Of Drought

Effects of drought stress on the seed germination and physiological characteristics of amaranth were investigated. The results were as follows: the germination rate and germination potential of amaranth decreased with the increasing of PEG-6000 concentrations. Meanwhile, the root length, shoot length and peroxidase (POD) activity were significantly increased at lower PEG-6000 concentrations, but then decreased with the increasing of PEG-6000. Malondialdehyde (MDA) content, proline content and superoxide dismutase (SOD) activity were all significantly increased under drought stress, and reached the top at 20% PEG-6000. These findings indicated that amaranth tolerates drought stress through increasing the activities of SOD and POD and accumulating proline content.

Results of potato varietieis for drought tolerance in vitro conditoin

2018 ◽  
Vol 22 (03) ◽  
pp. 72-76
Author(s):  
Enkhbold B ◽  
Ninjmaa O ◽  
Nyamgerel Kh
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Drought Tolerance ◽  
High Performance ◽  
Stressed Condition ◽  
Peg 6000 ◽  
Screening Experiment ◽  
Early Maturing ◽  
Potato Plantlet

The laboratory’s experiment conducted at IPAS in 2017. The goal of this study was to evaluate drought stress tolerance of released potato varieties using PEG-6000 in-vitro condition. Middle maturing variety Gala, early maturing Solist and Quarta were used experiment. The objectives were to determine how osmosis pressure affected potato plantlet height, root length, total weight of biomass, and stem diameter using their index comparing to normal condition. The screening experiment used three different treatments. The water stress treatments 0 MPa (control), -0.05 MPa and -0,15 MPa were created by PEG-6000. 6 weeks’ year’s old plantlets were measured by 5 features. The variety Gala showed significant high index by 5 performances among the stressed condition compared to Quarta and Solist. The variety Quarta also showed high performance compared Solist. As a result of this study found out that cultivar Gala more tolerant to drought than Quarta and Solist.

scholarly journals Regenerasi Tanaman dari Kalus Tebu yang Diiradiasi Sinar Gamma pada Medium dengan PEG 6000

2017 ◽  
Vol 44 (3) ◽  
pp. 322
Author(s):  
Agus Purwito ◽  
Nidya Ravenska ◽  
Dan Awang Maharijaya
Keyword(s):  
Drought Stress ◽  
In Vitro Selection ◽  
Gamma Ray ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Two Factors ◽  
Selection Medium ◽  
Gamma Ray Irradiation

ABSTRACT<br /><br />Sugarcane cultivars tolerant to drought stress are needed for specific location. In vitro selection can be used to obtain plants tolerant to drought stress through regeneration of callus in the culture medium. The purpose of this study was to obtain regenerants from irradiated callus on the stress medium. The experimental design was a completely randomized design with two factors. The first factor was the regeneration medium (RG) added with PEG 6000, i.e., 0%, 5%, 10% and 15%, while the second factor was the dose of gamma ray irradiation i.e. 0 krad, 5 and 10 krad, and 15 krad. There were 12 treatments, each treatment was repeated 18 times and each repetition was a culture bottles planted with three clums of callus 10 mm diameter. RG medium was the MS medium added with 0.5 mg L-1 BAP, Kinetin 0.1 mg L-1 and IBA 1.0 mg L-1. The higher the concentration of PEG, the less regenerants were produced. Regenerants could be generated from the selection medium PEG up to 15%. Several shoots still produced from callus irradiated with 10 krad. Selection medium with PEG up to 10% decreased the precentage of albino regenerants. The gamma ray irradiation dose and the concentration of PEG significanly affected the number of  roots produced.<br /><br />Keywords: drought tolerant, in vitro, polyethylene glycol, plantlets, albino<br /><br />

scholarly journals Physio-biochemical Responses of In Vitro Cooking Banana Musa paradisiaca cv Lang towards Pseudo Induced Drought Stress by Polyethylene Glycol (PEG)

2021 ◽  
Vol 17 (6) ◽  
pp. 805-817
Author(s):  
Sharifah Zafierah Syed Badrulzaman ◽  
Nurhana Nadia Ramlan ◽  
Munirah Adibah Kamarul Zaman ◽  
Azzreena Mohamad Azzeme
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Soluble Protein ◽  
Proline Content ◽  
Total Soluble Protein ◽  
Soluble Protein Content ◽  
Musa Paradisiaca ◽  
Cooking Banana ◽  
Total Soluble Protein Content

Musa paradisiaca cv Lang belongs to cooking banana group, and it has high potential to be used in banana chips production. Like other cultivars, M. paradisiaca cv Lang is susceptible towards water shortage, therefore affecting banana growth and productivity. In this study, to mimic the drought condition, pseudo-drought stress was given to in vitro Lang banana seedlings by adding polyethylene glycol (PEG). Overall, decrement of roots length and chlorophyll (Chl) content was displayed by the seedlings exposed to 1%, 2%, 3%, 4%, and 5% (w/v) of PEG after three weeks of exposure. The proline content, total soluble protein content, and antioxidant capacity in leaf and roots, however, countered differently towards different levels of drought. Proline content showed the highest in leaf of 2% (w/v) PEG-treated seedling (12.66±0.38 µmoles/g) while the total soluble protein content showed the highest in roots of 5% (w/v) of PEG-treated seedling (30.65±1.07 mg/g FW). Antioxidant capacity of stressed seedlings revealed the catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) activities were the highest in the leaf of 1% (w/v) (10.69±5.06 µmol/min/mg), 4% (w/v), (0.079±0.03 µmol/min/mg), and 5% (w/v) (9.11±8.47 µmol/min/mg) of PEG- treated seedlings, respectively. Meanwhile, the highest CAT, POD, and APX activities in the roots were determined in 3% (w/v) (0.49±0.04 µmol/min/mg), 2% (w/v) (0.03±0.02 µmol/min/mg), and 3% (w/v) (16.69±0.5 µmol/min/mg) of PEG-treated seedlings, respectively. These data show that PEG can be a priming agent to induce defense system at seedling stage of banana, which could enhance their survivability during ex vitro acclimatization.

scholarly journals Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
SYARIFAH AINI PASARIBU ◽  
Mohammad Basyuni ◽  
EDISON PURBA ◽  
YAYA HASANAH
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Morphological Characters ◽  
Sensitivity Index ◽  
Shoot Height ◽  
Peg 6000 ◽  
Rate Of Increase ◽  
Osmotic Solution

Abstract. Pasaribu SA, Basyuni M, Purba E, Hasanah Y. 2021. Drought tolerance selection of GT1 rubber seedlings with the addition of polyethylene glycol (PEG) 6000. Biodiversitas 22: 394-400. Tolerance of the rootstock of rubber to drought stress is not optimal. The root system can therefore be used as an indicator of drought tolerance to inhibit the growth of the plant. A good root system of rubber rootstocks is thought to help the plants acquire greater resistance to drought stress. Polyethylene glycol 6000 osmotic solution controls the water potential in the growing medium. This study aimed to analyze the effect of PEG 6000 osmotic solution (0%; 7.5%; and 15% concentration) on rubber seedlings leading to the development of morphological characters and sensitivity index. GT1 (Gondang Tapen 1) rubber seeds to be used as planting material were collected from the seed source garden of PT Socfin Indonesia. The study was carried out from January to February 2019, using a non-factorial randomized block design. The morphological characters observed were tapped root length, shoot height, the increased rate of taproot length, shoot height increase rate, ratio of taproot length, and shoot height. Data were analyzed using analysis of variance, discriminant, and drought stress sensitivity index. The results showed that the addition of PEG 6000 in planting media in vitro significantly influenced the character of the rate of increase of taproot length. The sensitivity index of taproot increase rate and the ratio of taproot length and shoot height was moderate at 7.5% levely contrast, the addition of PEG 6000 in planting media in vitro did not significantly impact it. The present study suggested that the rate of increase of taproot length was a distinguishing character showing the initial tolerance level of the GT1 seedlings to drought.

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