scholarly journals Purification and Identification of Total Flavonoids Extracted from Moringa oleifera Leaves in Iraq

2016 ◽  
Vol 10 (2) ◽  
pp. 73-80
Author(s):  
Nuha B. Khudaer ◽  
Zaynab Y. Muhammed Hassn ◽  
Khlood W. AL-Sammarrae ◽  
Nabeel K. Ibraheem
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Moringa Oleifera ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Plant Leaves ◽  
Leaf Extracts ◽  
Moringa Oleifera Leaves ◽  
Layer Chromatography ◽  
Important Medicinal Plant

Moringa oleifera is an important medicinal plant, which contains a lot of bioactive compounds. The dried plant leaves were extracted in 70% methanol by maceration using shaker incubator at 40◦C. Chemical detection of bioactive compounds in crud plant extracts was performed. The total flavonoid was isolated from the extract using reflux, The use of thin layer chromatography technique that assisted  in the detection of Qurciten, Rutin and Luteoline of flavonoids isolated from the extract, the existence of qurciten and rutin confirmed by the use of high performance liquid chromatography technique.  The calculation of total flavonoid of M. oleifera leaf extracts by using Spectrophotometric technique was done.  The aglycon moiety was extracted by ethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol.

scholarly journals Purification and Identification of Flavonoids Extracted from Loranthus Eurpaeus Fruits

2016 ◽  
Vol 10 (1) ◽  
pp. 10-15
Author(s):  
Eslah S. Rajab ◽  
Khlood W. Abood ◽  
Zaynab Y. Muhammed Hassn
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plant ◽  
High Performance ◽  
Methanolic Extract ◽  
Mobile Systems ◽  
Total Flavonoids ◽  
Layer Chromatography ◽  
Important Medicinal Plant ◽  
Qualitative Estimation

Loranthus eurpaeusis an important medicinal plant, which contains a lot of bioactive compounds. The dried plantfruits were extracted overnight with 80% methanol by maceration using shaker incubator 25c◦. Chemicaldetection of crud plant extracts was performed. The total flavonoids was isolated from the extract using reflux,and subjected to thin layer chromatography (TLC) using different mobile systems. The purified material wasaugmented by using high performance liquid chromatography (HPLC). The aglycon moiety was extracted byethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol. Purificationprocedures of flavonoid were fully described in this study. The aims of this study was to detect the activecompounds present in L. eurpaeus methanolic extract then quantitative and qualitative estimation of the totalflavonoids isolated from the plant fruits.

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 67-71
Author(s):  
R. C. Sutar ◽  
◽  
D. S Musmade
Keyword(s):  
High Performance ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

scholarly journals Evaluation of nutrient and antioxidant activity on steam blanching of Moringa oleifera leaves

2021 ◽  
Vol 306 ◽  
pp. 04016
Author(s):  
Ryzal Satria Aditama ◽  
Heri Ahmad Sukria ◽  
Rita Mutia
Keyword(s):  
Antioxidant Activity ◽  
Product Quality ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Moringa Oleifera ◽  
Total Flavonoid ◽  
Nutritional Content ◽  
Post Harvest ◽  
Moringa Oleifera Leaves

Moringa oleifera is a plant that has high nutritional content. Post-harvest treatment take effect in improving product quality. This study was carried out to evaluate nutrient and antioxidant activity of steam blanched moringa oleifera leaves. Sample of moringa oleifera were prepared where the temperature 80°C in ±5 minutes for the steam blanched. The fresh M. oleifera and steam blanched M. oleifera leaves dried at 60°C were analyzed for their proximate, mineral, total phenol, total flavonoid, and antioxidant activity contents. The analysis revealed that steam blanched leaves contained more dry matter, crude protein, crude fat, phenolic total, and flavonoid than unblanched leaves. Therefore, its less fibre and mineral. This study showed steam blanching can serve as alternative post-harvest treatment to increase nutritional value of moringa oleifera leaves.

scholarly journals Determination of flavones in species of Thymus L. (Lamiaceae) from Macedonian flora

2001 ◽  
Vol 47 ◽  
pp. 9-14
Author(s):  
Svetlana Kulevanova ◽  
Marina Stefova ◽  
Tatjana Kadifkova Panovska ◽  
Jasmina Tonic ◽  
Trajce Stafilov
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Gradient Elution ◽  
Hplc Method ◽  
Total Flavonoids ◽  
Column Temperature ◽  
Plant Samples ◽  
Layer Chromatography ◽  
Hydrolysis Of

Assay of flavonoids in extracts of seven Thymus L. (Lamiaceae) species from Macedonia including identification and quantification was performed. Extracts obtained after hydrolysis of air dried samples (A1) were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Luteolin and apigenin were identified in comparison to authentic standard substances. The content of total flavonoids in plant samples determined by UV-Vis spectrometry (with AlCl3) ranged from 0.05-0.13 %. Two other extracts were prepared by extraction with a mixture of ethanol:water (7:3, V/V), evaporation until only water remained and extraction first with diethylether (A2) and secondly with ethyl acetate (A3). The content of flavonoids in diethyl-ether and ethyl acetate extracts ranged from 52.5-244.4 mg·ml-1 and 48.7 -117.5 mg·ml-1, respectively. For quantification of luteolin and total flavonoids the HPLC method was applied, using reverse phase column C18, mobile phase consisting of 5% acetic acid and methanol in gradient elution mode and column temperature set to 40 o C. The content of luteolin in the plant samples ranged from 0.23-0.48 % (m/m), while the content of total flavonoids was found to be 0.26-0.52 %.

scholarly journals A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves

2021 ◽  
Vol 20 (11) ◽  
pp. 2371-2379
Author(s):  
Yanqin Zhu ◽  
Qinhong Yin ◽  
Yaling Yang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Moringa Oleifera ◽  
Accurate Determination ◽  
Gradient Elution ◽  
Hplc Method ◽  
Array Detector ◽  
Moringa Oleifera Leaves

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) and high performance liquid chromatography with ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD and UPLC-UV methods were applied for the accurate determination of eleven major polyphenols in Moringa oleifera leaves. The chromatographic conditions of the eleven polyphenols was determined on two C18 column by gradient elution with 0.5 % phosphoric acid solution -acetonitrile as the eluate, and at a flow rate of 1.0 and 0.5 mL/min for HPLC-DAD and UPLC-UV methods, respectively. Detector parameter of UPLC-UV was fixed at 203 nm. The assay methods were validated systematically.Results: The instrumental methods (HPLC-DAD and UPLC-UV) had good linearity, precision,repeatability and recovery. For both methods, quantification limits of UPLC-UV (0.057 - 0.363 μg/mL) were lower than those of UPLC-UV (0.094 - 1.532 μg/mL). The UPLC method with a shorter running time and more sensitive detection was applied in comparing to the HPLC method. After optimization and evaluation, the baseline of 11 compounds was separated effectively within 68 and 34 min, respectively.Conclusion: The developed HPLC-DAD and UPLC-UV assays were successfully utilized for thesimultaneous analysis of eleven major polyphenols and can readily be utilized as quality control tools for Moringa oleifera leaves in China, with UPLC-UV method showing better separation, lower organic solvent usage and shorter analytical period.

scholarly journals Evaluation of the Nematicidal and Antifungal Activity of Aqueous Extracts of Moringa oleifera Leaves and Seed in Cucumber Field

2018 ◽  
Vol 51 (4) ◽  
pp. 47-59
Author(s):  
M.C. Olajide ◽  
N.B. Izuogu ◽  
R.A. Apalowo ◽  
H.S. Baba
Keyword(s):  
Antifungal Activity ◽  
Meloidogyne Incognita ◽  
Moringa Oleifera ◽  
Organic Amendments ◽  
Fruit Weight ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Moringa Oleifera Leaves ◽  
Number Of Leaves

Abstract This aim of the two-year study was to evaluate the nematicidal and antifungal activity of Moringa oleifera extracts against Meloidogyne incognita and fungi infestation in cucumber field. The aqueous extracts of leaves and seeds of M. oleifera were used to treat the plants. The findings of the present study revealed that the plant extracts were active against the test pathogens. All treated plants were significantly higher than the control with respect to number of leaves and branches, vine length, fruit weight, and yield. Of the two varieties of cucumber used, combination of cucumber market with moringa aqueous leaf extracts gave higher results. The phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, saponins, and tannins. These possess nematicidal and antifungal activities. Combination of variety 2, Market More with Moringa leaves aqueous extract is being recommended to farmers for management of nematode and fungal diseases. Organic amendments have the advantage of controlling environmental effluence.

scholarly journals The Purification effect on Organoleptic Profile, Yield, Total Phenol and Total Flavonoids from 96% Ethanol Extract of Moringa (Moringa oleifera. L) leaves.

2020 ◽  
Vol 17 (2) ◽  
pp. 188-198
Author(s):  
Bayu Herdi Al Huda ◽  
Hari Susanti ◽  
Nining Sugihartini
Keyword(s):  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
One Way Anova

Purifikasi ekstrak etanol 96% daun kelor dilakukan untuk meningkatkan kandungan zat aktif serta untuk memperoleh warna yang lebih menarik. Tujuan penelitian ini adalah mengetahui jenis pelarut dalam proses fraksinasi yang memberikan kandungan zat aktif lebih tinggi dibandingkan ekstrak. Penelitian ini diawali dengan maserasi daun kelor kering dengan etanol 96%. Filtrat yang telah di keringkan di atas waterbath kemudian di purifikasi. Purifikasi diawali dengan melarutkan ekstrak ke aquades panas dengan perbandingan 1:10, setelah itu ditambahkan etil asetat atau n-heksana sejumlah sama dengan aquades panas dalam corong pisah, dikocok hingga terjadi pemisahan fase. Fase etil asetat atau n-heksana diambil kemudian filtrat yang diperoleh digabungkan. Ekstrak etanol 96% (E1), fraksi etil asetat (E2) dan fraksi n-heksana (E3) kemudian dievaluasi dengan parameter organoleptis, % rendemen, total fenol, dan total flavonoid. Data yang diperoleh dianalisis statistik one way ANOVA dengan taraf kepercayaan 95%. Hasil pengukuran terhadap E1, E2, dan E3 pada setiap parameter secara berturut-turut pada parameter rendemen adalah 15,81%; 22,18%; 20%, total fenol yaitu 28,94±0,54 mg GAE/g; 29,44 ± 0,93 mg GAE/g; 11,41±0,12 mg GAE/g, dan total flavonoid yaitu 3,19±0,13%; 9,92 ±0,06%; 5,81±0,18%. Secara organoleptis dari segi konsistensi, bau, dan rasa ketiganya adalah identik, sedangkan dari segi warna E2 lebih hijau kekuningan dan cerah, sehingga lebih menarik. Secara statistik, ada perbedaan bermakna di antara E1, E2, dan E3 pada total fenol dan flavonoid. Dapat disimpulkan bahwa E2 memiliki total fenol dan flavonoid lebih besar secara signifikan dibanding E1 dan E3.

scholarly journals Puree and Juice of Thai Mango and Pineapple Analyzed by High-Performance Thin-Layer Chromatography Hyphenated with Effect-Directed Assays

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7683
Author(s):  
Gertrud E. Morlock ◽  
Newitchaya Wutthinithisanand ◽  
Doris Rauhut
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
High Performance ◽  
Mangifera Indica ◽  
Quality Criterion ◽  
Health Claims ◽  
Ananas Comosus ◽  
Production Chain ◽  
Layer Chromatography

The requirements for analytical tools are changing due to the global production chain, the increasing cases of adulteration, and the growing trend towards consumption of plant-based food products worldwide. The assessment of bioactivity of natural foods is currently not a quality criterion, and a paradigm shift is postulated. A non-targeted effect-directed profiling by high-performance thin-layer chromatography hyphenated with five different effect-directed assays was developed exemplarily for the puree and juice products of mango Mangifera indica L. (Anacardiaceae) and pineapple Ananas comosus (L.) Merr. (Bromeliaceae). Several bioactive compounds were detected in each sample. The additional bioactivity information obtained through effect-directed profiles improves, expands and modernizes product control. Non-target effect-directed profiling adds a new perspective to previous target analysis results that can be used not only to ensure health claims based on bioactive compounds, but also to detect unknown bioactive compounds coming from contamination or residues or changes caused by food processing.

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