scholarly journals KAJIAN POTENSI EKSTRAK BUAH MENGKUDU (Morinda citrifolia) SEBAGAI DESINFEKTAN ALAMI TERHADAP JAMUR PADA RUANG PENYIMPANAN SUSU

2020 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Ratna Hadiyanti ◽  
Deden Zamzam Badruzzaman ◽  
Wowon Juanda ◽  
Yuli Astuti Hidayati
Keyword(s):  
Penicillium Digitatum ◽  
Morinda Citrifolia ◽  
Aspergillus Ochraceus ◽  
Noticeable Effect ◽  
Penicillium Purpurogenum ◽  
Storage Chamber ◽  
Storage Room ◽  
Before And After ◽  
Identification Of Fungi ◽  
Milk Storage

Morinda citrifolia can be used as a natural disinfectant on the milk storage space because it has an anti-fungal compound. The purpose of this study is to determine the potential of Morinda citrifolia extract as a natural disinfectant to fungi in the milk storage room, is reviewed from a decrease in the number of mushrooms and identification of the type of fungi before and after the use of Morinda citrifolia extracts. The study was conducted experimentally using a complete random design (CRD) with four treatments of P0 (without using Morinda citrifolia), P1 (15% concentration of Morinda citrifolia extract), P2 (20% concentration of Morinda citrifolia extract), P3 (concentration 25% of Morinda citrifolia extracts) and six times the replay. The observed is a decrease in the number of fungi, identifying the type of fungi before the use of Morinda citrifolia extract, identification of the type of fungi after the use of Morinda citrifolia extract. The results showed that Morinda citrifolia extract had a noticeable effect on decreasing the number of fungi in the milk storage room, with a consecutive drop percentage of P0 = 25,12%, P1 = 58,12%, P2 = 61,89%, P3 = 70,59%. The result of identification of fungi type on milk storage Chamber is Cephalosporium sp, Aspergillus ochraceus, Aspergillus flavus, Penicillium purpurogenum, Penicillium digitatum, Mucor sp. The decrease in the amount of the type of each fungus is Cephalosporium sp (68,13%), Aspergillus ochraceus (88%), Aspergillus flavu (87,94%), Penicillium purpurogenum (85,71%), Penicillium digitatum (95.73%), Mucor sp (59,57%).

scholarly journals Study of Noni (Morinda citrifolia) Extract Addition to The Quality of Catfish (Pangasius hypopthalmus) By Smoked Fish During Storage

Marinade ◽  
2018 ◽  
Vol 1 (01) ◽  
pp. 17-26
Author(s):  
Aidil Fadli Ilhamdy ◽  
Edison Edison ◽  
Sumarto Sumarto
Keyword(s):  
Water Content ◽  
Peroxide Value ◽  
Morinda Citrifolia ◽  
Value Analysis ◽  
Smoked Fish ◽  
Extract Concentration ◽  
Peroxide Values ◽  
Identification Of Fungi ◽  
Organoleptic Score

The experiment was conducted from to May-June 2010. Research purposes were to determine the effect of noni extract to improve the storability of catfish (Pangasius hypopthalmus) smoked fish during storage. Noni extract concentration used 0 kg, 1 kg, 2 kg and 3 kg. While groups of storage 0 days, 7 days, 14 days, 21 days and 28 days. Parameters measured were an organoleptic score, peroxide value, analysis of water content and identification of fungi. The results, from the identification of fungi using the parameter of noni extract 3 kg can be maintained for 28 days, with high organoleptic value and the low moisture and peroxide values.

scholarly journals Morinda citrifolia(Noni) as an Anti-Inflammatory Treatment in Women with Primary Dysmenorrhoea: A Randomised Double-Blind Placebo-Controlled Trial

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
H. M. Fletcher ◽  
J. Dawkins ◽  
C. Rattray ◽  
G. Wharfe ◽  
M. Reid ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Morinda Citrifolia ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Menstrual Cycles ◽  
Primary Dysmenorrhoea ◽  
Before And After ◽  
Laboratory Variables ◽  
Anti Inflammatory ◽  
Bleeding Score

Introduction. Noni (Morinda citrifolia) has been used for many years as an anti-inflammatory agent. We tested the efficacy of Noni in women with dysmenorrhea.Method. We did a prospective randomized double-blind placebo-controlled trial in 100 university students of 18 years and older over three menstrual cycles. Patients were invited to participate and randomly assigned to receive 400 mg Noni capsules or placebo. They were assessed for baseline demographic variables such as age, parity, and BMI. They were also assessed before and after treatment, for pain, menstrual blood loss, and laboratory variables: ESR, hemoglobin, and packed cell volume.Results. Of the 1027 women screened, 100 eligible women were randomized. Of the women completing the study, 42 women were randomized to Noni and 38 to placebo. There were no significant differences in any of the variables at randomization. There were also no significant differences in mean bleeding score or pain score at randomization. Both bleeding and pain scores gradually improved in both groups as the women were observed over three menstrual cycles; however, the improvement was not significantly different in the Noni group when compared to the controls.Conclusion. Noni did not show a reduction in menstrual pain or bleeding when compared to placebo.

scholarly journals An Engineering Approach to Storage and Access in High Priority Scenarios

2019 ◽  
Vol 63 (1) ◽  
pp. 1349-1353
Author(s):  
FNU Varun Ananthasivan Srikrishnan ◽  
Richard T. Stone ◽  
Cong Xu
Keyword(s):  
Human Factors ◽  
Human Performance ◽  
Normal Operation ◽  
Aviation Industry ◽  
Critical Systems ◽  
Safety Critical ◽  
Work Spaces ◽  
Storage Room ◽  
Before And After ◽  
Time Critical

Over the past few years, an extensive amount of research has been done in the field of Human Factors. Applications range from the design of day-to-day products like cell phones to the design and development of safety-critical systems like flight displays. The highly critical aviation industry has shown time and again the importance of human-centered approach in developing systems for the safety of those operating it and the passengers. Similarly, other safety-critical industries like law enforcement have been seen to incorporate human factors in the design of weapons and exoskeletons aimed at adapting to humans and making their unit stronger. Many manufacturing firms have begun to see the importance of proper work postures for their employees to avoid musculoskeletal disorders and the financial and regulatory implications of not following proper work ethics that take care of employees’ health. Further, many organizations have started to consider team dynamics in their operations understanding the importance of healthy interaction among the employees and between employees and the management. However, there are a very few references to any studies or organizational practices that draw a connection between human performance and human-centric re-design of work places, with most designs being limited to work desks and activity-based working (ABW) work spaces. This paper focuses on the organizational engineering of storage spaces to enable easy location and retrieval of equipment, thus supporting the time-critical nature of operations at a miscellaneous storage room at the Story County Sheriff’s Office. Experiments were carried out using two familiar scenarios both before and after the redesign of the storage room. A significant improvement in the performance of the operator was observed after the redesign, as could be seen by the reduction in time taken to identify and retrieve equipment and the qualitative survey that was obtained at the end of the experiment. The wasted time was translated to a cost and the newly designed storage design saved a significant amount of money spent on actions that precluded efficient accomplishment of tasks, something that could have been used by the Sheriff’s office to purchase equipment for normal operation of the office. The results suggested such interventions in different sectors that have similar high-priority operations. The results of the study indicates that there is a need for the industry to extend research towards this field that we name “organization engineering”.

scholarly journals IDENTIFICATION OF FUNGI ISOLATED FROM BATHROOMS IN FEMALE STUDENTS’ HOSTEL, UNIVERSITY OF BENIN, BENIN CITY

2021 ◽  
Vol 2 (2) ◽  
pp. 25-35
Author(s):  
C. L. Ozoaduche ◽  
I. B. Idemudia
Keyword(s):  
Candida Albicans ◽  
Aspergillus Nidulans ◽  
Environmental Changes ◽  
Public Health Importance ◽  
Benin City ◽  
Significant Difference ◽  
Mucor Mucedo ◽  
Before And After ◽  
Identification Of Fungi ◽  
Microbiological Techniques

Fungi are specifically dangerous as they exhibit a significant tolerance to environmental changes and can proliferate under low relative humidity, unlike bacteria. They produce spores that are easily dispersed by air hence they are ubiquitous. The study aimed at identifying the fungal isolates present in the bathrooms located on the three floors of the hostel, University of Benin, Benin City. Samples were collected from the walls of the bathrooms using sterile swab sticks and were identified using standard microbiological techniques. The isolated fungi were Aspergillus nidulans, A. niger, A. tamarii, A.  flavus, Candida albicans, Penicillium cyclopium, P. oxalicum, Mucor mucedo, Trichophyton rubrum and Rhodotorula species. From the ground floor bathrooms, Candida albicans (23.40%) were most frequently isolated, Aspergillus nidulans (55.56%) were mostly isolated from the first floor and Mucor mucedo (56.00%) were the most isolated from the second floor.  After washing the bathrooms, Mucor mucedo was scarcely isolated from the walls of the bathrooms. The findings were processed statistically using the two-tailed test to detect the significant difference between the groups of means for the fungal counts from each floor. A significant difference (p<0.05) in the fungi isolated before and after washing was found. Isolated fungi from this study are known to be of public health importance hence, strict hygiene practices should be observed by those using the bathrooms.

scholarly journals Exploring the potential of fungal-bacterial consortium for low-cost biodegradation and detoxification of textile effluent

2016 ◽  
Vol 42 (4) ◽  
pp. 12-21 ◽  
Author(s):  
Harshad Lade ◽  
Avinash Kadam ◽  
Diby Paul ◽  
Sanjay Govindwar
Keyword(s):  
Hplc Analysis ◽  
Low Cost ◽  
Bacterial Consortium ◽  
Textile Effluent ◽  
Aspergillus Ochraceus ◽  
Time Period ◽  
Before And After ◽  
Cost Treatment ◽  
Treatment Technologies ◽  
Reduced Toxicity

AbstractIn the present study, the enrichment and isolation of textile effluent decolorizing bacteria were carried out in wheat bran (WB) medium. The isolated bacterium Providencia rettgeri strain HSL1 was then tested for decolorization of textile effluent in consortium with a dyestuff degrading fungus Aspergillus ochraceus NCIM 1146. Decolorization study suggests that A. ochraceus NCIM 1146 and P. rettgeri strain HSL1 alone re moves only 6 and 32% of textile effluent American Dye Manufacturing Institute respectively in 30 h at 30 ±0.2°C of microaerophilic incubation, while the fungal-bacterial consortium does 92% ADMI removal within the same time period. The fungal-bacterial consortium exhibited enhanced decolorization rate due to the induction in activities of catalytic enzymes laccase (196%), lignin peroxidase (77%), azoreductase (80%) and NADH-DCIP reductase (84%). The HPLC analysis confirmed the biodegradation of textile effluent into various metabolites. Detoxification studies of textile effluent before and after treatment with fungal-bacterial consortium revealed reduced toxicity of degradation metabolites. The efficient degradation and detoxification by fungal-bacterial consortium pre-grown in agricultural based medium thus suggest a promising approach in designing low-cost treatment technologies for textile effluent.

LITERATUR REVIEW : EFEKTIVITAS BUAH MENGKUDU (Morinda Citrifolia L.) TERHADAP PENURUNAN HIPERTENSI

2021 ◽  
Vol 13 (02) ◽  
pp. 128
Author(s):  
Roikatul Miskiyah ◽  
Friska Realita
Keyword(s):  
Blood Pressure ◽  
Treatment Group ◽  
Systolic Blood Pressure ◽  
Statistical Test ◽  
Morinda Citrifolia ◽  
Control Group ◽  
P Value ◽  
Fruit Extract ◽  
Before And After ◽  
Interaction Factors

ABSTRAKLatar Belakang : Hipertensi adalah The Silent Disease sehingga seseorang tidak akan mengetahui jika dirinya menderita hipertensi atau tekanan darah tinggi. Meningkatnya tekanan darah sistolik lebih besar dari 120 mmHg dan diastolik lebih besar dari 80 mmHg pada dua kali pengukuran dengan selang waktu 5 menit dalam keadaan cukup istirahat disebut dengan Hipertensi, Hal tersebut timbul karena adanya banyak faktor interaksi resiko yang dimiliki seseorang. Tujuan : Penelitian ini bertujuan untuk menganalisis efektivitas buah mengkudu dalam menurunkan hipertensi. Metode : Pencarian artikel nasional ini dilakukan dengan basis data seperti google scohlar. Dengan kata kunci: “hipertensi”.”ekstrak buah mengkudu”. Dan penulis menemukan 7 artikel yang relevan 2014-2018. masing-masing mewakili efektivitas buah mengkudu terhadap penurunan hipertensi terhadap semua sampel.  Hasil  : Tujuh artikel yang di dapat, Menunjukkan hasil uji statistik  p Value 0,015 (p<0,05). berarti ada pengaruh antara tekanan darah siastolik maupun diastolik penderita hipertensi sebelum dan sesudah diberikan air perasan buah mengkudu. Kesimpulan : tekanan darah pada kelompok perlakuan sesudah intervensi buah mengkudu mengalami penurunan tekanan darah dibandingkan dengan kelompok control, efektivitas buah mengkudu ialah salah satu pengobatan non farmakologis.Kata kunci: Hipertensi, Ekstrak Buah MengkuduTHE EFFECTIVENESS OF NONI FRUIT (MORINDA CITRIFOLIA L.)    TO REDUCE HYPERTENSIONABSTRACTBackground : Hypertension is The Silent Disease so that a person will not know if he has hypertension or high blood pressure. Increased systolic blood pressure greater than 120 mmHg and diastolic greater than 80 mmHg in two measurements with an interval of 5 minutes in a state of sufficient rest is called Hypertension. This arises because there are many risk interaction factors that a person has. Objective : This study aims to analyze the effectiveness of noni fruit in reducing hypertension. Methods : This national article search was carried out using databases such as Google Scohlar. With keywords: "hypertension", "noni fruit extract". And the author found 7 relevant articles 2014-2018. each represents the effectiveness of noni fruit in reducing hypertension in all samples. Results : Seven articles were obtained, showing the results of the statistical test p Value of 0.015 (p <0.05). This means that there is an influence between systolic and diastolic blood pressure in patients with hypertension before and after being given noni juice. Conclusion : blood pressure in the treatment group after the noni fruit intervention decreased blood pressure compared to the control group, the effectiveness of noni fruit is one of the non-pharmacological treatments.Keywords: Hypertension, Mengkudu Fruit Extract.

scholarly journals ISOLASI DAN IDENTIFIKASI CENDAWAN TERBAWA BENIH KAKAO HIBRIDA

2020 ◽  
Vol 18 (1) ◽  
pp. 40
Author(s):  
BAHARUDIN BAHARUDIN ◽  
AGUS PURWANTARA ◽  
SATRIYAS ILYAS ◽  
MOHAMAD RAHMAD SUHARTANTO
Keyword(s):  
Fusarium Oxysporum ◽  
Theobroma Cacao ◽  
Potato Dextrose Agar ◽  
Aspergillus Ochraceus ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Randomized Design ◽  
Seedborne Fungi ◽  
Identification Of Fungi ◽  
Fungi Infection

<p>ABSTRAK<br />Benih merupakan komponen dasar dalam menentukan produktivitas<br />tanaman kakao. Benih yang sehat dapat merupakan faktor penting dalam<br />menentukan keberhasilan produktivitas kakao. Benih kakao mempunyai<br />kadar air cukup tinggi sehingga berpotensi terinfeksi cendawan, yang<br />dapat menurunkan mutu benih dan produksi kakao. Penelitian bertujuan<br />untuk mengisolasi dan mengidentifikasi beberapa cendawan terbawa benih<br />pada kakao hibrida. Penelitian dilakukan di Kebun Induk Benih Pusat<br />Penelitian Kopi dan Kakao Indonesia, Jember, Laboratorium Mikro-<br />biologi, Balai Penelitian Bioteknologi Perkebunan Indonesia, dan<br />Laboratorium Pengendalian Hayati IPB Bogor pada bulan Juni sampai<br />Oktober 2008. Penelitian menggunakan benih kakao hibrida dari hasil<br />persilangan buatan antar TSH 858 dengan Sca 6, dan percobaan disusun<br />dengan rancangan acak lengkap dengan 3 ulangan. Benih ditumbuhkan<br />pada 3 media, yaitu water agar (WA), potato dextrose agar (PDA), dan<br />kertas saring (KS). Tingkat infeksi pada benih diamati setiap hari dan<br />dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji selang<br />berganda Duncan. Cendawan diisolasi, dibiakkan, dimurnikan, dan<br />diidentifikasi dengan menggunakan buku kunci identifikasi. Tingkat<br />infeksi cendawan terbawa benih kakao hibrida tertinggi terdapat pada hari<br />keempat (35,00%) dan kelima (51,67%) pada media PDA. Sebanyak 13<br />spesies cendawan terbawa benih kakao hibrida berhasil diidentifikasi<br />dengan menggunakan media WA dan PDA, serta 8 spesies cendawan<br />dengan media KS. Ke-13 cendawan terbawa benih yang ditemukan sangat<br />berpotensi menurunkan mutu fisiologis benih dan produktivitas kakao.<br />Cendawan tersebut perlu diuji lebih lanjut karena masing-masing memiliki<br />sifat-sifat patogenik, saprofitik, atau antagonistis terhadap cendawan lain<br />pada benih kakao. Cendawan terbawa benih kakao hibrida paling dominan<br />adalah Aspergillus spp., Penicillium chrysogenium, Coletotrichum<br />acutatum, Curvularia geniculata, dan Fusarium spp. Cendawan-cendawan<br />yang diduga berbahaya adalah Aspergillus spp., Coletotrichum acutatum,<br />Curvularia  geniculata,  Fusarium  spp.,  Phoma  glomerata,  dan<br />Macrophoma sp., dan yang diduga bersifat patogenik adalah Aspergillus<br />flavus, Aspergillus ochraceus, Cladosporium herbanum, Curvularia<br />geniculata, Fusarium oxysporum, Phoma glomerata, dan Macrophoma sp.</p><p>Kata kunci : Theobroma cacao, benih hibrida, infeksi cendawan, media<br />tanam</p><p>Isolation and Identification of Fungi on Hybrid Cacao Seeds</p><p>ABSTRACT<br />Seed is the basic component influencing the productivity of cacao<br />plantation. Healthy seed is the most important factor in determining the<br />success of cacao productivity. Moisture content of cacao seeds is quite<br />high potentially to cause fungi infection, which can further reduce seed<br />quality and cacao production. The research aimed at isolating and<br />identifying several seedborne fungi on hybrid cacao. The study was<br />conducted at main nursery of Indonesian Coffee and Cocoa Research<br />Institute Jember, Laboratory of Microbiology, Indonesian Biotechnology<br />Research Institute for Estate Crops, and the Laboratory for Biological<br />Control of IPB Bogor from June to October 2008. Research used hybrid<br />cacao seeds derived from crossing between TSH 858 x SCA 6, and the<br />experiment was arranged using completely randomized design with three<br />replicates. Cacao seeds were grown on three media, i.e. water agar (WA),<br />potato dextrose agar (PDA), and filter paper (KS). Infection rates on the<br />seedlings were observed every day and analyzed using ANOVA followed<br />by Duncan's multiple regression test (DMRT). Fungi were isolated,<br />cultured, purified, and identified using the identification keys. The highest<br />rate of seedborn fungal infection occured on fourth (35.00%) and fifth<br />(51.67%) days on PDA media. A total of 13 species of seedborn fungi on<br />hybrid cocoa were identified by using WA and PDA media, as well as 8<br />other species by using KS. The 13 seedborne fungi potentially reduce seed<br />physiological quality and cacao productivity. These fungi need to be<br />further tested because each has its own pathogenic, saprophytic, or<br />antagonistic properties towards other fungi on cacao seeds. Predominant<br />seedborn fungi on hybrid cacao were Aspergillus spp., Penicillium<br />chrysogenium, Coletotrichum acutatum, Curvularia geniculata, and<br />Fusarium spp. The fungi suspected harmful were Aspergillus spp.,<br />Coletotrichum acutatum, Curvularia geniculata, Fusarium spp., Phoma<br />glomerata, and Macrophoma sp., and those suspected pathogenic were<br />Aspergillus flavus, Aspergillus ochraceus, Cladosporium herbanum,<br />Curvularia geniculata, Fusarium oxysporum, Phoma glomerata, and<br />Macrophoma sp.<br />Key words : Theobroma cacao, fungi infection, hybrid seed, growing<br />media</p>

scholarly journals Formulasi Salep Ekstrak Buah Mengkudu (Morinda citrifolia L.) Kombinasi Zeolit Alam Lampung (Zal) Sebagai Penstabil Sediaan Antibakteri Staphylococcus aureus

2020 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Laila Susanti ◽  
Lilik Koernia Wahidah ◽  
Pratika Viogenta
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Natural Zeolite ◽  
Morphological Structure ◽  
Morinda Citrifolia ◽  
P Value ◽  
Synthetic Drugs ◽  
Before And After ◽  
Alternative Drug ◽  
Inhibition Zones

ABSTRAK Sediaan salep esktrak buah mengkudu telah terbukti sebagai antibakteri, namun esktrak tanaman pada umumnya sangat mudah terdegradasi oleh pH, cahaya dan suhu, oleh karena itu pemanfaatan Zeolit Alam Lampung (ZAL) dengan keistimewaan struktur morfologinya diharapkan mampu mempertahankan kestabilan sediaan salep hingga suhu tinggi. Penelitian ini bertujuan untuk mengetahui potensi ZAL sebagai penstabil sediaan salep dengan menghitung perubahan prosentase zona hambat bakteri pada sediaan salep sebelum dan sesudah dilakukan penyimpanan pada suhu 40oC. Penelitian ini meliputi tiga tahap utama yakni pertama formulasi salep ekstrak buah mengkudu kombinasi Zeolit Alam Lampung (ZAL) dengan variasi konsentrasi ekstrak 10%+ZAL, 15%+ZAL, 30%+ZAL, KZ (Kontrol Zeolit tanpa esktrak) dan KE (Kontrol Ekstrak tanpa zeolit) yang kemudian dilakukan evaluasi sediaan salep. Tahap kedua seluruh sampel dilakukan uji aktivitas antibakteri terhadap bakteri Staphylococcus aureus pada sediaan salep, dan tahap ketiga seluruh sampel disimpan pada suhu 40oC selama 24 jam, kemudian diuji aktivitas antibakteri kembali terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa seluruh sampel sebelum dilakukan penyimpanan pada suhu 40oC menunjukkan respon zona hambat bakteri yang kuat kecuali KZ. Setelah dilakukan penyimpanan pada suhu 40oC, terjadi penurunan zona hambat pada KE dan Ekstrak 10%+ZAL sebesar 100% dan 36,81%. Hal yang menarik terjadi pada Esktrak 15%+ZAL dan 30%+ZAL, yakni terjadi kenaikan zona hambat sebesar 35,20 % dan 31,32%. Hasil uji statistik menunjukkan nilai Asymp. Sig (0,016) <  nilai p (0,01) maka H0 ditolak sehingga dilakukan uji lanjut Tukey yang menunjukkan terjadi perbedaan yang signifikan antar kelompok uji. Dari penjelasan tersebut maka dapat disimpulkan bahwa ZAL mampu meningkatkan kinerja sediaan salep ekstrak buah mengkudu terutama pada konsentrasi 15% dan 30% dalam menghambat bakteri Staphylococcus aureus sehingga sediaan salep esktrak buah mengkudu kombinasi ZAL dapat digunakan sebagai alternatif obat pengganti obat sintetik kimiawi.  Kata kunci : Zeolit, Salep, M.citrifolia L, Staphylococcus aureus ABSTRACT             Noni extract ointment has been proven to be antibacterial, but plant extracts are generally very easily degraded by pH, light and temperature, therefore the use of Lampung Natural Zeolite (ZAL) with its special morphological structure is expected to be able to maintain the stability of ointment preparations to high temperatures. This study aims to determine the potential of ZAL as a stabilizer for ointment preparations by calculating the change in the percentage of bacterial inhibition zones in ointment preparations before and after storage at 40oC. This study includes three main stages, namely the first formulation of noni extracts from Lampung Natural Zeolite extract (ZAL) with variations in extract concentration of 10% + ZAL, 15% + ZAL, 30% + ZAL, KZ (Zeolite control without extract) and KE (Control Extract without zeolite) which then evaluated the ointment preparation. The second stage of all samples was tested for antibacterial activity against Staphylococcus aureus bacteria in ointment preparations, and the third stage all samples were stored at 40oC for 24 hours, then tested for antibacterial activity against Staphylococcus aureus. The results showed that all samples before storage at 40oC showed a strong inhibitory zone response except KZ. After storage at 40oC, there was a decrease in inhibition zones at KE and Extract 10% + ZAL by 100% and 36.81%. An interesting thing happened at the Extract 15% + ZAL and 30% + ZAL, namely an increase in inhibition zones of 35.20% and 31.32%. Statistical test results indicate the Asymp value. Sig (0.016) >p value (0.01) then H0 is accepted, so Tukey's further tests show that there are significant differences between the test groups. From this explanation it can be concluded that ZAL can improve the performance of noni fruit extract ointment especially at concentrations of 15% and 30% in inhibiting Staphylococcus aureus bacteria so that the ZAL noni extract extract can be used as an alternative drug for chemical synthetic drugs. Keywords : Zeolite, Ointment, M.citrifolia L, Staphylococcus aureus

scholarly journals Enhancement Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression of Macrobrachium rosenbergii Fed With Aqueous Morinda citrifolia Leaves Extract-Supplemented Diet

2018 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Atika Marisa Halim ◽  
Arief Prajitno ◽  
Chin Chyuan Chang
Keyword(s):  
Gene Expression ◽  
Glutathione Peroxidase ◽  
Antioxidant Properties ◽  
Macrobrachium Rosenbergii ◽  
Morinda Citrifolia ◽  
Immune Gene ◽  
Gene Expressions ◽  
Immune Gene Expression ◽  
Before And After ◽  
Gpx Activity

Morinda citrifolia, known commercially as noni is often used for enhance immunity, these plant rich of phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed with diets containing of aqueous M. citrifolia leaves extract (AMLE) at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx) activity was conducted to measure immune parameter, these parameter was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. Immune gene expression of α2-macroglubulin (α2-M) was evaluated in this study. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Gene expressions of α2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii. Keyword: Morinda citrifolia, Macrobrachium rosenbergii, GPx, α2-M

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