Efek Ekstrak Metanol dan Partisi dari Kulit Batang Kayu Jawa (Lannea coromandelica Houtt. Merr.) terhadap Pertumbuhan Sel HeLa dan MCF-7

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Nadyah Haruna ◽  
Zakiah Anugerah Hamzah ◽  
Syamsuri Syakri ◽  
Isriany Ismail ◽  
Nursalam Hamzah
Keyword(s):  
Mtt Assay ◽  
Lannea Coromandelica ◽  
Mcf 7

Tujuan penelitian ini adalah menentukan efek ekstrak metanol dan partisi kulit batang kayu jawa dalam menghambat pertumbuhan sel kanker HeLa dan sel kanker MCF-7. Simplisia kulit kayu dimaserasi menggunakan pelarut metanol. Ekstrak kemudian dipartisi secara partisi cair padat menggunakan pelarut heksan. Pemisahan menghasilkan partisi larut heksan dan partisi tidak larut heksan. Pengukuran aktivitas inhibisi pertumbuhan sel dilakukan menggunakan metode MTT Assay terhadap sel HeLa, sel MCF-7 dan sel Vero. Senyawa yang dikandung diidentifikasi dengan beberapa pereaksi golongan. Hasil penelitian menunjukkan bahwa ekstrak metanol, partisi tidak larut heksan dan partisi larut heksan dapat menghambat pertumbuhan sel kanker Hela dengan nilai indeks selektivitas, berturut-turut, 2,04; 1,85 dam 4,19. Sedangkan untuk sel MCF-7, indeks selektivitas, berturut-turut, 1,91; 1,44 dan 2,13. Partisi larut heksan dapat memiliki potensi sebagai antikanker dengan selektivitas yang baik.

scholarly journals Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity

Crystals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 571
Author(s):  
Ahmed Gaber ◽  
Walaa F. Alsanie ◽  
Majid Alhomrani ◽  
Abdulhakeem S. Alamri ◽  
Ibrahim M. El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carboxylic Acid ◽  
Anticancer Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Anticancer Effect ◽  
Reference Compound ◽  
Anticancer Effects ◽  
Mcf 7

This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b, 7c, 8a, 8b, and 8c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b, 7c, 8a, 8b, and 8c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.

Biological Effects of p53 Mutation W248 and H175 on MCF-7 Cells

2013 ◽  
Vol 790 ◽  
pp. 550-554
Author(s):  
Xiang Yu Zhou ◽  
Ya Jun Liu ◽  
Dan Li
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Cell Migration ◽  
Tumor Cell ◽  
Mtt Assay ◽  
Biological Effects ◽  
Scratch Test ◽  
Control Group ◽  
Tumor Cell Migration ◽  
Mcf 7

Objective: p53, a tumor suppressor gene, is one of the hotspots in the world of the biomedical field. Mutation of p53 gene, which is found in approximately 50% of human cancers, is a key event in carcinogenesis. This project aims to investigate the new characteristics of two p53 mutants, p53-W248 and p53-H175, in MCF-7 cells, so as to provide the experimental basis for understanding the functional alternations of mutant p53. Methods: In this study, MCF-7 cells transfected with p53-H175 or p53-W248 plasmids were used as experimental group and the MCF-7 cells transfected wild type p53 plasmid were used as control group. Then the biological effects at the cellular level were investigated using 3-(4.5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, flow cytometry analysis and cell scratch test. Results: MTT assay showed that p53-W248 might promote cell proliferation in MCF-7 cells. The results of flow cytometry indicated that no significant effect on cell cycle progression and cell apoptosis by p53-H175 or p53-W248 in cells. The cell scratch test showed that p53-H175 could increase the ability of cell migration. Conclusion: p53-H175 could lead to the promotion of tumor cell migration, while p53-W248 may promote tumor cell proliferation. p53-H175 and p53-W248 might have acquired some new characteristics of oncogenes.

scholarly journals Cytotoxic Constituents of Pachyrhizus Tuberosus from Peruvian Amazon

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Olga Leuner ◽  
Jaroslav Havlik ◽  
Milos Budesinsky ◽  
Vladimir Vrkoslav ◽  
Jessica Chu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Human Cancer ◽  
Chemical Constituents ◽  
Human Fibroblasts ◽  
Cancer Cell Lines ◽  
Cytotoxic Effects ◽  
Hct 116 ◽  
Mcf 7

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 μg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 μg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 μg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 μg/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

scholarly journals High performance thin-layer chromatography and in vitro cytotoxic studies on ethanol extract of Matricaria chamomilla L (Asteraceae) flowers

2021 ◽  
Vol 18 (9) ◽  
pp. 1969-1976
Author(s):  
Mohammed Al Bratty ◽  
Lalitha K. Govindaram ◽  
Lalitha K. Govindaram ◽  
Neelaveni Thangavel ◽  
Hassan A. Alhazmi ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mtt Assay ◽  
High Performance ◽  
Cytotoxic Effect ◽  
Ethanol Extract ◽  
Matricaria Chamomilla ◽  
Layer Chromatography ◽  
Mcf 7

Purpose: To develop a high performance thin-layer chromatography (HPTLC procedure for quantitation of apigenin in ethanol extract of Matricaria chamomilla (Babunaj) flowers, and to evaluate the extract for in vitro cytotoxic effect on MCF-7 cell lines. Methods: Quantification of apigenin was carried out using a CAMAG TLC system. A combination of toluene, ethyl acetate and formic acid (4.5:3.5:0.2 v/v/v) was used as mobile phase, with densitometry detection at 336 nm. The HPTLC procedure was subjected to validation as per ICH guidelines. The cytotoxicity of the extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: A sharp apigenin band at Rf of 0.51 was obtained, and the content of apigenin in the extract was 0.062 % w/w. The detection limit (LOD) and quantification limit (LOQ) were 0.19 and 0.57 ng/band, respectively. MTT assay results indicate that M. chamomilla was cytotoxic to Michigan Cancer Foundation-7 (MCF-7) cells, with half-maximal concentration (IC50) of 74 µg/mL. Conclusion: The developed HPTLC method is linear, precise, accurate and specific for the determination of apigenin. M. chamomilla exerts cytotoxic effect on MCF-7 cell line via induction of apoptosis.

scholarly journals Triterpenoids with Cytotoxic Potential from the Leaves of Tridax procumbens L.

2017 ◽  
Vol 9 (4) ◽  
pp. 597
Author(s):  
Vaishali Rai ◽  
Vinitha Ramanath Pai ◽  
Surya Ram Duwal
Keyword(s):  
Breast Cancer ◽  
Molecular Weight ◽  
Mtt Assay ◽  
Crude Extract ◽  
Breast Cancer Cell Lines ◽  
Phytochemical Analysis ◽  
Major Peak ◽  
Cytotoxic Potential ◽  
Tridax Procumbens ◽  
Mcf 7

<p align="left">The anticancer activity of crude extracts of the leaves of <em>Tridax procumbens, </em>against two breast cancer cell lines-MCF-7 (benign) and MDA-MB-231 (metastatic) were investigated and an attempt was made to identify the anticancer principle. The extracts with methanol (TPM), ethanol (TPE) and chloroform (TPC) as solvents were screened for cytotoxicity by MTT assay against MCF-7 cells. The effective extract was further evaluated on MDA-MB-231 cells. Among the three extracts, TPC was effective at an IC<sub>50</sub> value of 136 µg/ml and 129 µg/ml on MCF-7 and MDA-MB-231 cells respectively. Phytochemical analysis of the extract showed the presence of only steroids and terpenoids and their concentration was high (77.4% w/w). HPLC-MS of the chloroform soluble crude extract revealed a major peak (57.59% concentration) at a retention time of 4.78 min and MS data of this peak revealed presence of two fragments of molecular weight 475.80 and 701.80. The compounds were identified to be 3β, 9β-Dihydroxy-18α-oleanan-28-oic acid, a derivative of oleanolic acid and 3β, 28-Bis (cinnamoyl) betulin respectively, both from the triterpenoid family. </p>

scholarly journals Development, Characterization Optimization, and Assessment of Curcumin-Loaded Bioactive Self-Nanoemulsifying Formulations and Their Inhibitory Effects on Human Breast Cancer MCF-7 Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1107
Author(s):  
Mohsin Kazi ◽  
Fahd A. Nasr ◽  
Omar Noman ◽  
Abdulrahman Alharbi ◽  
Mohammed S. Alqahtani ◽  
...  
Keyword(s):  
Droplet Size ◽  
Mtt Assay ◽  
Oral Delivery ◽  
Seed Oil ◽  
Drug Loading ◽  
Drug Candidate ◽  
Lipid Digestion ◽  
Black Seed ◽  
Black Seed Oil ◽  
Mcf 7

Curcumin (CUR) is an attractive polyphenol for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties. Poor solubility in water and sensitivity against sunlight are the most challenging characteristics in the development of CUR for clinical use. The aim is to develop oral lipid-based bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs) for curcumin as a candidate for cancer therapy. Bio-SNEDDSs containing black seed oil, medium-chain mono- and diglycerides, and surfactants were prepared as CUR delivery vehicles. The morphology, droplet size, physical stability, encapsulation efficiency, risk of precipitation, lipid digestion, antioxidant activity, and antimicrobial activity were evaluated for the representative formulations. Finally, an MTT assay was performed on MCF-7 cells to determine the cytotoxic effect of the different formulations. The results showed lower droplet size (28.53 nm) and higher drug-loading (CUR 20 mg, thymoquinone 1.2 mg) for the representative Bio-SNEDDS (black seed oil/Imwitor 988/KolliphorEL (35/15/50) % w/w), along with a transparent appearance upon aqueous dilution. The dynamic dispersion and in-vitro lipolysis data proved that the Bio-SNEDDS was able to keep the CUR in a solubilized form in the gastrointestinal tract. From the antioxidant and antimicrobial studies, it was suggested that the Bio-SNEDDS had the highest activity for disease control. The MTT assay showed that the representative Bio-SNEDDS treatment led to a reduction of cell viability of MCF-7 cells compared to pure CUR and conventional SNEDDSs. A Bio-SNEDDS with elevated entrapment efficiency, antioxidant/antimicrobial activities, and an antiproliferative effect could be the best anticancer drug candidate for potential oral delivery.

scholarly journals Sixteen New Prenylated Flavonoids from the Fruit of Sinopodophyllum hexandrum

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3196 ◽  
Author(s):  
Yanjun Sun ◽  
Haojie Chen ◽  
Junmin Wang ◽  
Meiling Gao ◽  
Chen Zhao ◽  
...  
Keyword(s):  
Hepg2 Cell ◽  
Mtt Assay ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
Cytotoxic Activities ◽  
Prenylated Flavonoids ◽  
Ic50 Values ◽  
Sinopodophyllum Hexandrum ◽  
Hepg2 Cell Lines ◽  
Mcf 7

Sixteen new prenylated flavonoids, sinoflavonoids P–Z (1–11) and sinoflavonoids NA–NE (12–16), were isolated from the fruit of Sinopodophyllum hexandrum, along with eight known analogues (17–24). Their structures were elucidated on the basis of extensive spectroscopic data (HR-ESI-MS, 1H-NMR, 13C-NMR, HSQC, HMBC). The cytotoxic activities of compounds 1–18, 20, and 22 were evaluated by MTT assay. Compound 6 showed the most potent cytotoxicity in MCF-7, and HepG2 cell lines, with IC50 values of 6.25 and 3.83 μM, respectively.

scholarly journals CYTOTOXIC ACTIVITY OF BRAZILEIN ISOLATED FROM SECANG (CAESALPINIA SAPPAN L.) AGAINST MCF7/DOX CELLS BY INHIBITION OF P-GLYCOPROTEIN

2017 ◽  
Vol 9 (12) ◽  
pp. 124 ◽  
Author(s):  
N. P. Linda Laksmiani ◽  
E. D. Y. Meiyanto ◽  
R. Asmah Susidarti
Keyword(s):  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
In Silico ◽  
Cancer Resistance ◽  
Caesalpinia Sappan ◽  
P Glycoprotein ◽  
13C Nuclear Magnetic Resonance ◽  
Mcf 7

Objective: This study was focused on isolation of brazilein from the dried heartwood of Secang (Caesalpinia sappan L.) followed by its characterization using Infrared (IR) spectroscopy, liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI), proton (1H), carbon-13 (13C) nuclear magnetic resonance (NMR) and two dimensional (2D)-NMR, evaluation the cytotoxic activity of brazilein in MCF-7 resistant doxorubicin (MCF-7/DOX) cells and evaluate the interaction between brazilein and ATP with P-glycoprotein (Pgp) in silico using molecular docking.Methods: Brazilein was isolated and purified from ethyl acetate fraction by flash silica gel column chromatography, eluting with chloroform, ethyl acetate and methanol in gradient concentration. In the cytotoxicity assay, MCF-7/DOX cells were cultured in the presence of brazilein for 24 hour (h) and cell viability was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Interactions between brazilein and the target proteins were evaluated and calculated in silico by molecular docking using PLANTS.Results: The infrared, mass spectra with a molecular weight of 284 and NMR signal confirmed that was brazilein. MTT assay showed a dose-dependent inhibition of MCF-7/DOX cell proliferation with brazilein IC50 value of 43 µM. The docking score of brazilein was-71,45 kcal/mol and ATP value-96,23 kcal/mol.Conclusion: Brazilein has a potent cytotoxic value on MCF-7/DOX and high affinity in Pgp protein target. Brazilein can be developed as anticancer especially in cancer resistance incidence. Further study must be established to evaluate the molecular mechanism of brazilein inhibit MCF-7/DOX cell proliferation in vitro.

scholarly journals ANTIOXIDANT AND ANTICANCER ACTIVITY OF METHANOLIC EXTRACT FROM STEPHANIA ELEGANS

2017 ◽  
Vol 9 (2) ◽  
pp. 245 ◽  
Author(s):  
Ravin Sharma ◽  
Gourav Chandan ◽  
Anterpreet Chahal ◽  
Reena V. Saini
Keyword(s):  
Mtt Assay ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Isolation And Characterization ◽  
Mcf 7

<p class="Default"><strong>Objective: </strong>The present work was aimed to investigate the <em>in vitro </em>antioxidant and anti-cancer activities of methanolic extract of <em>Stephania elegans</em>, an unexplored species from <em>Menispermaceae</em> family.</p><p class="Default"><strong>Methods: </strong>The methanolic extract of <em>S. elegans </em>tubers was prepared and phytochemical screening and total phenolic content were analyzed by using standard methods. <em>In </em><em>vitro,</em><em> </em>antioxidant potential of methanolic extract was determined by 2-2’-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) assays. Cytotoxicity against human breast cancer cell line, Michigan Cancer Foundation-7 type (MCF-7) was evaluated by 3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) (MTT) assay.</p><p class="Default"><strong>Results: </strong>Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, carbohydrates, tannins, terpenoids, steroids, and saponins in the methanolic extract of <em>S. elegans </em>tubers. The total phenolic content in the methanolic extract was 23.0±0.06 mg GAE/g (dry mass). As revealed by ABTS assay, the methanolic extract of plant tubers showed significant radical scavenging activity (IC<sub>50</sub> 41.66±0.015 μg/ml). The reducing power activity of the extract increased with the concentration of the extract. MTT assay indicated that <em>S. elegans </em>has potent cytotoxic activity towards MCF-7 cells (IC<sub>50 </sub>158.7±0.13 μg/ml).</p><p class="Default"><strong>Conclusion: </strong>This is the first study demonstrating the antioxidant and anticancer capabilities of the methanolic extract of <em>S. elegans</em>. This study also provides a significant basis for further isolation and characterization of bioactive compounds from <em>S. elegans</em>.</p>

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