scholarly journals Quality assessment of pea (Pisum sativum L.) seeds using the controlled deterioration technique

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Md. Abdullahil Baque 2 ◽  
Md. Lutfor Rahman 1 ◽  
Md. Mahfuzar Rahman 1 ◽  
Md. Mahmudul Hasan 1 ◽  
Md. Ahsan Habib 2 ◽  
...  

A three-factor experiment was set at the Horticulture Laboratory, Hajee Mohammad Danesh Science and Technology University, Dinajpur to study the effects of the controlled deterioration (CD) on the pea seeds at the constant temperature of 350C. The 3 factors considered were: 3 pea seed sources (Rangpur Local/RL, Dinajpur Local/DL and Thakurgaon Local/TL); 3 ageing periods (0, 8 and 16 days); and 3 seed moisture contents (12, 16 and 20% MC). The 27 treatment combinations compared in the CRD with the 3 repetitions for the 8 arenas were: % germination, % abnormal seedlings, % dead seeds, % soil emergence and seedling evaluation test for the root and shoot lengths as well as their dry matter contents. Identical prototypes of notable (5-1% level) degradations were recorded everywhere. But the disparities were lucid under the extreme stresses. Moreover, highly noteworthy (1% level) relations were traced amid all the traits ranging from -0.9847 (soil emergence × abnormal seedling) to 0.9623 (soil emergence × normal seedling). So, the CD technique was very effectual in judging the physiological statuses of the seed sources studied. Thus, the germination test might be add-on by a vigor test, the latter of which could be assessed by quantifying the seedlings’ root and shoot lengths and/or their dry matter accumulations. Moreover, in the seed quality certification, the suitable limits of vigor for the chosen traits could also be got by this technique. But the seeds of several pea varieties should be exploited to fix-up the agreeable limits of the traits. Furthermore, to save time, the ageing period could be squeezed by raising the seed MC.       

Author(s):  
Luo Qinjuan ◽  
Wang Lianming ◽  
Zhao Xiaoqing ◽  
Qian Hua ◽  
Yan Lei

Rapid and noninvasive detection methods of seed vigor, an important index to evaluate seed quality, have been the research focus in recent years. In this paper, the detection method of pea seed vigor based on hyperspectral imaging technology was researched. First, the spectral images of different vigor grade samples with artificial aging were captured, and the original spectrum was pretreated with multiple scattering correction. Secondly, SPA and PCA were used to select respective bands. Finally, PLS-DA and LS-SVM model were established to identify the seed vigor of the pea seed, based on the whole band spectrum, the characteristic bands extracted by SPA and PCA respectively. The results showed that PLS-DA and LS-SVM models are effective, but LS-SVM had better performance. Through comparison, the method using full band spectrum was more accurate, the efficiency of method using 5 characteristic bands extracted by PCA was the highest while the way of extracting the representative band by SPA was the most meaningful to this study which achieved similar accuracy to the full band with only 20 bands. The SPA-LS-SVM method afforded the recognition accuracy (100%) for modeling set and validation set used to determine the vigor of pea seeds. The overall results suggest that hyperspectral imaging technology is useful for classification of different vitality pea seeds with non-destructive manner, which can provide a basis for further development of online scoring devices


2013 ◽  
Vol 35 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Patrícia Pereira da Silva ◽  
Raquel Alves de Freitas ◽  
Warley Marcos Nascimento

The objectives of this study were to evaluate the efficiency of fungicides for pea seed treatment against damping-off caused by Rhizoctonia solani and to verify their effects on physiological seed quality. 'Mikado' pea seeds were treated with the following fungicides: Carbendazim, Carbendazim + Thiram, Captan, Iprodione, Iprodione + Thiram, Metalaxyl-M + Fludioxonil, Pencycuron, Procymidone and Tolyfluanid. Control seeds were treated with deionized water. Physiological seed quality was evaluated with the following tests: germination, first count, accelerated aging and electrical conductivity. Seeds were sown in soil inoculated and no inoculated with R. solani. The experimental design was completely random with four replications. Seedling emergence was reduced in inoculated soil and the best treatments for R. solani control were Carbendazim, Pencycuron, Iprodione and Carbendazim + Thiram. Captan reduced seed physiological quality in both the laboratory and field.


1992 ◽  
Vol 2 (1) ◽  
pp. 9-15 ◽  
Author(s):  
R. H. Ellis ◽  
C. Pieta Filho

AbstractSeed development and changes in germination ability and longevity were monitored in two spring and two winter cultivars of each of barley (Hordeum vulgare L.) and wheat(Triticum aestivum L.). Physiological maturity (end of the seed-filling period) occurred between 31 and 41 d after 50% anthesis in the eight cultivars, at which time seed moisture contents had declined naturally to 40–49% (wet basis). Most seeds were able to germinate (in 28 d tests at 10°C) following enforced desiccation at this time (normal germination varied from 83% in one cultivar of winter wheat to 97% in one of spring wheat).Potential seed longevity continued to increase after physiological maturity. The intervalbetween physiological maturity and the time when seeds attained maximum potential longevity varied between 3 and 21 d; maturation drying had reduced seed moisture contents to 16–28% (wb) at this time. The results for all eight cultivars contradict the hypothesis that maximum seed quality is attained at physiological maturity and thereafter declines. The term physiological maturity is potentially misleading, therefore; mass maturity is more appropriate to describe this developmental stage. It is shown that the results of accelerated ageing or controlled deterioration tests may provide misleading conclusions in seed quality development studies if the single storage treatment chosen is inappropriate.


1998 ◽  
Vol 8 (4) ◽  
pp. 437-443 ◽  
Author(s):  
Henk Jalink ◽  
Rob van der Schoor ◽  
Angela Frandas ◽  
Jaap G. van Pijlen ◽  
Raoul J. Bino

AbstractA method is proposed for assessing the maturity and quality of seeds, based on measuring the amount of chlorophyll fluorescence (CF) signals of intact seeds. In general, the amount of chlorophyll is directly related to the degreening process and thus the maturity. Cabbage seeds (Brassica oleracea var. capitata) were separated into three subsamples based on the CF signals of the individual intact seeds. Seeds with the lowest amount of CF had the highest percentage of germination and normal seedlings. In a controlled deterioration test, the subsample with the lowest CF signal had slightly lower germination and normal seedling percentages than the non-treated seeds, whereas the seeds with the highest CF signals had much lower germination and normal seedling percentages. Advantages of the CF method for determining seed maturity and seed quality are its high sensitivity and fully non-destructive nature and the high speed at which the fluorescence is generated and measured.


Author(s):  
Vanessa Vernoud ◽  
Ludivine Lebeigle ◽  
Jocelyn Munier ◽  
Julie Marais ◽  
Myriam Sanchez ◽  
...  

Abstract The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart an undesirable bitter taste when used as an ingredient in foodstuffs. In this paper, we describe the identification and characterization of a gene involved in saponin biosynthesis during pea seed development, by screening mutants obtained from two Pisum sativum TILLING (Targeting Induced Local Lesions in Genomes) populations in two different genetic backgrounds. The mutations studied are located in a gene designated PsBAS1 (β-amyrin synthase1) which is highly expressed in maturing pea seeds and which encodes a protein previously shown to correspond to an active β-amyrin synthase. The first allele is a nonsense mutation, while the second mutation is located in a splice site and gives rise to a mis-spliced transcript encoding a truncated, non-functional protein. The homozygous mutant seeds accumulated virtually no saponin without affecting seed nutritional or physiological quality. Interestingly, BAS1 appears to control saponin accumulation in all other tissues of the plant examined. These lines represent a first step in the development of pea varieties lacking bitterness off-flavours in their seeds. Our work also shows that TILLING populations in different genetic backgrounds represent valuable genetic resources for both crop improvement and functional genomics.


1992 ◽  
Vol 72 (3) ◽  
pp. 635-641 ◽  
Author(s):  
A. E. Van Deynze ◽  
P. B. E. McVetty ◽  
R. Scarth ◽  
S. R. Rimmer

To compare the effects of varying seeding rate on the agronomic performance, phenology and seed quality of hybrid and conventional summer rape cultivars, four hybrid and two conventional summer rape cultivars were seeded at 1.5, 3.0, 4.5, 6.0 and 9.0 kg ha−1 at two locations for 3 yr. The hybrid cultivars were, very importantly, 24% higher yielding and produced 50% more total dry matter than the conventional cultivars. The hybrid cultivars were, on average, 1.3% lower in seed oil content, 1.0% higher in seed protein content and equal in sum of oil and protein in the seed compared with the conventional cultivars. The hybrid cultivars were on average, 1.3 d later to 50% flowering and 1.1 d later to maturity than the average for the conventional cultivars, (i.e., equal or earlier to flowering and maturity than Regent). The hybrid cultivars were also 3.9% lower in harvest index and 1.3 ppm lower in chlorophyll content than the conventional cultivars. In spite of these differences, there were no significant cultivar-by-seeding-rate interactions, indicating that the hybrid and conventional cultivars responded similarly to varying seeding rate. Lodging, days to 50% flowering, days to maturity, harvest index, survival, oil content and protein content displayed significant linear responses to varying seeding rate. Stand at maturity, seed yield and total dry matter production displayed significant linear and quadratic responses to varying seeding rate. Varying seeding rate had no effect on seed formation period, the sum of oil and protein content, or chlorophyll content. A seeding rate of 6 kg ha−1 maximized seed yield for both hybrid and conventional summer rape cultivars.Key words: Brassica napus, canola, seed quality, agronomy, phenology


1989 ◽  
Vol 69 (3) ◽  
pp. 749-761 ◽  
Author(s):  
ULRICH HERTER ◽  
JOSEPH S. BURRIS

Mechanical drying has frequently caused injury in corn seed. Changes in seed moisture, temperature, and quality were determined for inbred lines A632, B73 and Mo17 to define the relationship between these variables. Ears harvested at ca. 48 and 38% seed moisture could be dried at 50 °C for 4–15 h and 18–24 h, respectively, before germination started to decline linearly with prolonged 50 °C drying. Drying time at 50 °C, seed moisture, or embryo moisture after 50 °C drying could be used equally well for prediction of seed quality. Seedling dry weights often declined even when seed was dried for only a few hours at 50 °C. Temperature measurements within seeds indicated that evaporation cooled the seed no more than 5 °C. Drying susceptibility of seed parents varied greatly between years.Key words: Moisture, temperature changes, seed corn, drying


2015 ◽  
Vol 37 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Priscilla Brites Xavier ◽  
Henrique Duarte Vieira ◽  
Cynthia Pires Guimarães

The aim of this study was to assess the effect of different coatings on the physiological potential of stylosanthes cv. Campo Grande seeds. The treatments were: uncoated seeds; limestone + PVA glue; limestone + sand + PVA glue; limestone + activated carbon + PVA glue; calcium silicate + PVA glue; calcium silicate + sand + PVA glue; calcium silicate + activated carbon + PVA glue. Posteriorly, the seeds were analyzed for water content (WC), maximum diameter (MAD) and minimum diameter (MID), thousand seed weight (TSW), germination test, germination speed index (GSI), mean germination time (MGT), emergence, emergence speed index (ESI), mean emergence time (MET), shoot and root length, fresh and dry matter of shoot and root. The coating increased the TSW, MAD and MID and decreased its WC. The treatments comprising limestone + PVA glue and limestone + sand + PVA glue increased the germination time, but none of the treatments negatively affected the physiological seed quality. Treatment with calcium silicate + PVA glue was outstanding for germination speed index and fresh and dry matter of shoot and root in the stylosanthes cv. Campo Grande seeds coating.


1987 ◽  
Vol 27 (1) ◽  
pp. 179 ◽  
Author(s):  
MA Siddique ◽  
G Somerset ◽  
PB Goodwin

Trials on the cultivars Canyon and Gallatin 50 in 1978 and Cascade in 1979 were run in North Queensland to examine ways of improving seed quality of snap beans. The trials concentrated on the maturation period, since this is a critical period for the development of seed quality. We found that seed quality was poor when the crop was cut at the stage when the leaves had fallen and all the pods were dry, or if the plants were cut at any stage and allowed to dry on the ground in single rows. This poor seed quality was associated with high pod temperatures during seed maturation. Cutting the crop before leaf fall, at a seed moisture content close to 50% (20-40% of pods dry) and windrowing immediately in 5 or 10 rows to 1 windrow gave low pod temperatures during seed maturation and high seed quality. Seed harvested and threshed directly off the crop was of good quality provided the seed moisture content in the crop had fallen to less than 25%.


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