scholarly journals Microclimate-based mortality analysis of the Cibodas Botanic Gardens plant collection

2021 ◽  
Vol 21 (3) ◽  
pp. 142-149
Author(s):  
IMAWAN WAHYU HIDAYAT ◽  
DIDIK WIDYATMOKO
Keyword(s):  
Wind Velocity ◽  
Pearson Correlation ◽  
Heavy Rain ◽  
P Value ◽  
Strong Wind ◽  
Botanic Gardens ◽  
Plant Collections ◽  
Pearson Correlation Test ◽  
Plant Collection ◽  
Multiple Regression Modeling

Despite the routinely conducted treatment and maintenance of garden plant collection, the monthly mortality figures of the Cibodas Botanic Gardens (CBG) plant collections remain significant. It is presumed that the microclimate has a crucial influence on plant survivorship in the field. This study aimed to analyze the effect of microclimate conditions on the mortality rate of the CBG plant collection. The study was conducted by correlating the number of mortal plants with CBG monthly microclimatic conditions from 2017 to 2018. The analyzed microclimate parameters were temperature, relative humidity, precipitation, wind velocity, and solar radiation. The multiple regression modeling, t-test, and Pearson correlation test (r) were utilized to measure the level of significance of the correlation (α = 0.05). The findings were shown that the maximum wind velocity was the primary unit correlated to the number of mortality. The correlation was strong positive (r = 68.8%), and significant (p-value α). We suggested anticipating the disadvantages influences of strong wind supported by other extreme microclimate units, such as heavy rain. These events frequently caused high damage to the tree and other plant collections. These are expected to be taken into a consideration by the CBG operator and management in order to forecast and mitigate the risks of future plant collection losses.

scholarly journals Hubungan kadar HbA1C dengan estimasi filtrasi glomerulus pada pasien DM tipe 2

e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Rixi Gahung ◽  
Karel Pandelaki ◽  
Emma Sy. Moeis
Keyword(s):  
Diabetes Mellitus ◽  
Hba1c Level ◽  
Pearson Correlation ◽  
P Value ◽  
Type 2 Dm ◽  
High Hba1c ◽  
Pearson Correlation Test ◽  
Relationship Of ◽  
The Relationship

Abstract: Diabetes mellitus is still increasing in number in the future. Type 2 diabetes mellitus type 2 (T2DM) often appears in adults and the cause is due to a shortage of the hormone insulin on a relative basis, generally appears slowly and gradually worsens. One of the parameters to control blood sugar level is HbA1c. The high HbA1c increases the risks and complications in T2DM patients. This study aimed to obtain the relationship between HbA1c level and GFR among T2DM patients. This was an analytical study using the patient's medical record data. This study was conducted at the Endocrine Clinic and Heart and Hypertension Clinic with a total samples of 65 patients. The results showed that of the 65 subjects, there were 40 male patients and 25 female patients. The Pearson correlation test showed that the relationship of the HbA1c level and GFR had a p value = 0.05 > 0.462 and the correlation r = 0.093. Conclusion: There was no significant relationship between HbA1c level and GFR in type 2 DM patients. Keywords: HbA1c, GFR, type 2 DM Abtsrak: Diabetes merupakan suatu penyakit tidak menular yang akan terus meningkat jumlahnya di masa datang. Diabetes Melitus tipe 2 (DMT2) sering muncul pada orang dewasa dan penyebabnya oleh karena kekurangan hormon insulin secara relatif , umumnya muncul perlahan dan secara bertahap akan bertambah berat. Salah satu parameter kontrol kadar gula darah ialah HbA1c. Jika HbA1c meningkat terjadi pula peningkatan resiko komplikasi. Penelitian ini bertujuan untuk mengetahui hubungan kadar HbA1c dengan eLFG pada pasien DMT2. Jenis penelitian ini analitik dengan mengevaluasi data rekam medik pasien. Penelitian dilakukan di Poliklinik Endokrin dan Poliklinik Jantung dan Hipertensi dengan jumlah subyek penelitian 65 pasien DMT2. Hasil penelitian ini menunjukkan bahwa dari 65 orang subjek terdapat 40 laki-laki dan 25 perempuan. Untuk mengetahui adanya hubungan kadar HbA1c dengan LFG digunakan analisis bivariat Pearson correlation yang mendapatkan nilai p hitung = 0,462> 0,05 dengan kekuatan korelasi r = 0,093. Simpulan: Tidak terdapat hubungan bermakna antara kadar HbA1c dengan eLFG pada pasien DM tipe 2.Kata kunci: HbA1c, eLFG, DM tipe 2

scholarly journals Anode heel effect application with step wedge against effect of signal to noise ratio in computed radiography

2020 ◽  
Vol 4 (3) ◽  
pp. 75-82
Author(s):  
Ni Nyoman Ratini ◽  
I Made Yuliara ◽  
Windaryoto Windaryoto
Keyword(s):  
Computed Radiography ◽  
Signal To Noise Ratio ◽  
Pearson Correlation ◽  
P Value ◽  
Signal To Noise ◽  
Correlation Test ◽  
Pearson Correlation Test ◽  
R Value ◽  
Noise Ratio ◽  
Heel Effect

It was researched on the application of Anoda Heel Effect (AHE) with a step wedge on the effect of Signal To Noise Ratio (SNR) on Computed Radiography (CR) has been carried out. This research was conducted on a 21 step wedge with two treatments, namely the application of AHE and without the application of AHE. This measurement is repeated three times on radiographs to obtain a total image of six images (without the application of AHE as many as three images and with the application of AHE as many as three images). The results of taking radiographs using AHE and without AHE were measured using the RadiAnt Dicom Viewer program. The SNR value on the step wedge image without the AHE application has an average of 26.89. The SNR value on the step wedge image using AHE is 60.54. The results of the correlation test (Pearson correlation test) on the SNR showed that there was a significant and very strong effect of the application of AHE on the step wedge on the SNR in CR (p-value <0.001 and the R-value ranging from 0.600 to 0.799).

scholarly journals HUBUNGAN TINGKAT PENGETAHUAN ORANG TUA DENGAN KEBERSIHAN GIGI DAN MULUT ANAK DI TK TUNAS BHAKTI MANADO

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Triska Yolanda Worang ◽  
Damajanti H. C. Pangemanan ◽  
Dinar A. Wicaksono
Keyword(s):  
Oral Hygiene ◽  
Domain Knowledge ◽  
Pearson Correlation ◽  
Sampling Technique ◽  
Parental Knowledge ◽  
The Elderly ◽  
P Value ◽  
Cross Sectional ◽  
Pearson Correlation Test ◽  
Level Of Knowledge

Abstract: Domain knowledge is very important for the formation of one's actions. Knowledge of parents is very important in the formation of the underlying behaviors that support or do not support the oral hygiene of children. Good oral hygiene will make healthy teeth and surrounding tissues. This study aims to analyze the relationship between the level of knowledge of parent swith achild's dental and oral hygiene in kinder garten Tunas Bhakti Manado. The research used the descriptive analytical study with cross sectional approach. The experiment was conducted in a kinder garten classroom Shoots Bhakti Manado. The sample in this study all children in kinder garten preschool Tunas Bhakti Manado as many as 70 children examined OHI-S and as many as 12 questions questionnaire for the elderly. Sampling technique with a total sampling method.The results of this study indicate that parental knowledge about dental and oral hygiene in either category by 45.7% with oral hygiene status of children included in the medium category at 65.7%. Based on the results obtained Pearson correlation test p value of 0.020 (p <0.05). It can be concluded that there is a relationship between the level of knowledge of parents with children's dental and oral hygiene in kindergarten Tunas Bhakti Manado. Keywords: knowledge, OHI-S, preschoolers.   Abstrak: Pengetahuan merupakan domain yang sangat penting untuk terbentuknya tindakan seseorang.Pengetahuan orang tua sangat penting dalam mendasari terbentuknya perilaku yang mendukung atau tidak mendukung kebersihan gigi dan mulut anak. Kebersihan mulut yang baik akan membuat gigi dan jaringan sekitarnya sehat. Penelitian ini bertujuan untuk menganalisis hubungan antara tingkat pengetahuan orang tua dengan kebersihan gigi dan mulut anak di TK Tunas Bhakti Manado.Jenis penelitian yang digunakan yaitu penelitian deskriptif analitik dengan pendekatan cross sectional study.Penelitian dilaksanakan di ruangan kelas TK Tunas Bhakti Manado. Sampel dalam penelitian ini seluruh anak prasekolah di TK Tunas Bhakti Manado sebanyak 70 anak diperiksa OHI-S dan kuesioner sebanyak 12 pertanyaan untuk orang tua. Teknik pengambilan sampel dengan metode total sampling. Hasil penelitian ini menunjukkan bahwa pengetahuan orang tua mengenai kebersihan gigi dan mulut dalam kategori baik sebesar 45,7% dengan status kebersihan gigi dan mulut anak termasuk dalam kategori sedang sebesar 65,7%. Berdasarkan hasil uji korelasi pearson didapatkan p value 0,020 (p<0,05). Dapat disimpulkan bahwa terdapat hubungan antara tingkat pengetahuan orang tua dengan kebersihan gigi dan mulut anak di TK Tunas Bhakti Manado. Kata kunci: pengetahuan, OHI-S, anak prasekolah.  

scholarly journals Hubungan Pola Asuh Orang Tua terhadap Perkembangan Moral Siswa Kelas Tinggi Di SD Negeri Cimpon Desa Tirtosari Kecamatan Kretek Kabupaten Bantul Tahun Ajaran 2014/2015

2018 ◽  
Vol 8 (2) ◽  
pp. 75
Author(s):  
Khanif Maksum ◽  
Shofia Khusni Winasih
Keyword(s):  
Elementary School ◽  
Data Collection ◽  
Moral Development ◽  
Pearson Correlation ◽  
Simple Random Sampling ◽  
P Value ◽  
Pearson Correlation Test ◽  
Strongly Agree ◽  
Academic Year ◽  
Parenting Pattern

<p>Shofia Khusni Winasih: "The Relationship between Parenting Paterns and Moral Development of Upper Grades Students at Cimpon State Elementary School Tirtosari Village, Kretek District, Bantul Regency in Academic Year of 2014-2015". Essay. Yogyakarta: Bachelor Program Madrasah Ibtidaiyah Teacher Education, ALMA ATA Islamic Higher Education, 2015.</p><p><em>            </em>This research is a survey research that takes sample from one population and uses questionnaire as the main data collection tool. The population in this study are 46 upper grades students and their parents Cimpon State Elementary School in Academic Year of 2014-2015. Samples are taken by using simple random sampling is which sample members of the population are taken randomly without any regard to the stratas in the population. Variables in this study consists of variable X (Parenting pattern from upper grades students’ parents) and variable Y (Moral Development the upper grades students). The data collection techniques used in this research are obsevation, questionnaires, interviews, and documentation. Meanwhile, questionnaire were taken by using likert scale instrument: Strongly Agree (SS), Agree (S), Neutral / Doubt (N), Disagree (TS), Strongly Disagree (STS).</p><p>The results of this research shows that 3 parents (6.52%) used uthoritarian parenting patern and all of their children, three children (100%), showed less moral development. Parents who adopted democratic parenting were 43 parents (93.48%) and most of their children, 23 children (53.5%), showed adequate moral development and the rest of the children, 20 children (46.5%), showed good moral development. Most of the upper grades students showed adequate moral development (23 children or 50%); incontrast, children with less moral development were found as the smallest number (3 children or 6.5%).</p><p>The result of Pearson correlation test obtained the value of X2 -0.380 and were significant at 0.009 (p-value &lt;0.05). This means that there is a relationship between parental patterns to children moral development in upper grades students in Cimpon Elementary School, Tirtosari Village, Kretek District, Bantul Regency in Academic Year of 2014/2015. In other words, this research hypothesis can be proven or accepted.</p><p>Keyword: <em>Parenting Pattern, moral development.</em></p>

scholarly journals Gambaran Tingkat Kecemasan dan Kadar Hormon Kortisol Ibu Nifas

2021 ◽  
Vol 3 (2) ◽  
pp. 55-63
Author(s):  
Ni Putu Dian Ayu Anggraeni ◽  
Baiq Eka Putri Saudia
Keyword(s):  
Pearson Correlation ◽  
Simple Random Sampling ◽  
Elisa Test ◽  
P Value ◽  
Depression Symptoms ◽  
Cross Sectional ◽  
Multiparous Women ◽  
Pearson Correlation Test ◽  
Cortisol Levels ◽  
Postpartum Mothers

Introduction: Physical, social, mental, and psychological issues are common in the first few days after giving birth. According to some research, postpartum depression symptoms are linked to high cortisol levels. Postpartum psychological stress causes higher cortisol levels. Objective: The aim of this study was to look at postpartum mother's anxiety and cortisol levels. Methods: An Analytical Survey with a Cross-Sectional Approach is used in this analysis. With simple random sampling, there were 38 samples that met the inclusion criteria. Data was collected using the PSAS questionnaire for anxiety and an ELISA test for cortisol levels. Univariate and Pearson correlation test is used in the data analysis. Results: The majority of postpartum mothers reported mild anxiety in the first 24 hours after childbirth, with an average cortisol level of 10.034ng/ml 47.4%. The average level of the puerperal hormone cortisol in primiparous women was 14.60ng/ml higher than in multiparous women 7.42 ng/dl. Conclusion: With a p-value of 0.017, there was a substantial difference in cortisol levels between primiparous and multiparous postpartum mothers. With a p-value <0.001, there was a close connection between anxiety scores and cortisol levels. Future studies would need to focus on making it easier for postpartum mothers to feel less anxious.

scholarly journals HUBUNGAN KADAR ZINK DALAM ASI DENGAN BERAT BADAN DAN PANJANG BADAN BAYI USIA 4-6 BULAN DI KOTA PADANG SUMATERA BARAT

2019 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
Ratnadewi Ratnadewi
Keyword(s):  
Public Health ◽  
Zinc Deficiency ◽  
Pearson Correlation ◽  
Public Health Problem ◽  
Simple Random Sampling ◽  
Zinc Level ◽  
P Value ◽  
Cross Sectional ◽  
Correlation Test ◽  
Pearson Correlation Test

Zinc deficiency is public health problem. Almost two million people in developing country suffered zinc deficiency. Zinc deficiency in Indonesia, 25% in breastfeeding mothers and 17% in babies. The purpose of this study was to determine the correlation of zinc level in breastmilk with 4-6 months old baby weight and length in Padang City West Sumatera. This study was done in working area of Lubuk Buaya, Padang Pasir, and Nanggalo Public Health Center. This study was done from January 2016 until January 2017. The design of this study was cross sectional, number of sample were 108 people. Samples were selected by simple random sampling. Zinc level in breastmilk was checked by AAS (Atomic Absorption Spectrophotometer). Data were analyzed by using univariate and bivariate with pearson correlation test. The correlation test, correlation was positive and patterned between log zinc level in breastmilk with 4-6 months old baby weight (r=0,272), while the length (r=0,216), correlation was positive and weak with p value <0,05. That there were significant correlation between log zinc level in breastmilk and 4-6 months old baby weight and length.

scholarly journals HUBUNGAN PEMANFAATAN DANA BANTUAN OPERASIONAL KESEHATAN TERHADAP PENINGKATAN CAKUPAN PEMERIKSAAN KEHAMILAN DAN PERSALINAN DI FASILITAS KESEHATAN

2020 ◽  
Vol 11 (1) ◽  
pp. 79-88
Author(s):  
Suparmi Suparmi ◽  
Iram Barida Maisya ◽  
Anissa Rizkianti ◽  
Ika Saptarini ◽  
Ario Baskoro
Keyword(s):  
Public Health ◽  
Maternal Health ◽  
Linear Regression ◽  
Antenatal Care ◽  
Health Workers ◽  
Pearson Correlation ◽  
Health Facilities ◽  
P Value ◽  
Correlation Test ◽  
Pearson Correlation Test

Abstract Background: Health Operational Assistance (BOK) is a central government fund allocation to support the operation of Health Center (Puskesmas) to increase promotive and preventive activities related to public health, including maternal health. Objective: This study aims to determine the relationship of BOK utilization on maternal health service coverage. Method: The study used secondary data from the Ministry of Health e-Renggar Planning and Budget Bureau, Statistics Indonesia, the Ministry of Finance and the 2018 Public Health Development Index (IPKM) report. The unit of analysis is districts. Bivariate analysis was performed by Pearson correlation test, while multivariate analysis was performed by linear regression using SPSS software version 15. Results: The result of Pearson correlation test showed that BOK utilization was positively correlated with an increase in the antenatal care coverage (r = 0.294; p-value = 0,000) and coverage of deliveries by health workers in health facilities (r = 0.227; p-value = 0,000). The results of linear regression analysis showed BOK utilization, the percentage of poverty and the adequacy of posyandu have a significant relationship with antenatal care coverage and coverage of delivery assisted by health workers in health facilities. Conclusion: The health operational fund utilization increase antenatal care coverage and coverage of delivery assisted by health workers in health facilities. Therefore, it is necessary to optimize the use of BOK for the achievement of priority public health programs, especially for innovative activities that have a high degree of leverage towards the achievement of SDGs so that the target in reducing Maternal Mortality Rate (MMR) can be achieved. Keywords: health operational fund, maternal health, coverage, services   Abstrak Latar Belakang: Bantuan Operasional Kesehatan (BOK) merupakan salah satu sumber pembiayaan dari pemerintah pusat untuk menunjang operasional Puskesmas dalam meningkatkan upaya promotif dan preventif terkait kesehatan masyarakat, salah satunya kesehatan ibu. Tujuan: Studi ini bertujuan untuk mengetahui hubungan pemanfaatan BOK terhadap cakupan pelayanan kesehatan ibu. Metode: Studi ini menggunakan data sekunder dari laporan e-renggar Biro Perencanaan dan Anggaran Kementerian Kesehatan, Badan Pusat Statistik, Kementerian Keuangan dan laporan Indeks Pembangunan Kesehatan Masyarakat (IPKM) tahun 2018. Unit analisis adalah Kabupaten/Kota. Analisis bivariate dilakukan dengan uji korelasi pearson, sedangkan analisis multivariate dilakukan dengan regresi linier menggunakan software SPSS versi 15. Hasil: Hasil analisis uji korelasi pearson menunjukkan bahwa persentase realisasi BOK berkorelasi positif dengan peningkatan cakupan pemeriksaan kehamilan K4 (r=0,294; p-value=0,000) dan cakupan persalinan oleh tenaga kesehatan di fasilitas kesehatan (r=0,227; p-value=0,000). Hasil analisis regresi linier menunjukkan pemanfaatan BOK, persentase penduduk miskin dan kecukupan posyandu mempunyai hubungan bermakna dengan cakupan K4 dan cakupan persalinan ditolong oleh tenaga kesehatan di fasilitas kesehatan. Kesimpulan: Pemanfaatan BOK berhubungan dengan peningkatan cakupan K4 dan cakupan persalinan ditolong oleh tenaga kesehatan di fasilitas kesehatan. Optimalisasi pemanfaatan BOK untuk pencapaian program prioritas kesehatan masyarakat diperlukan, terutama untuk kegiatan-kegiatan inovatif yang memiliki daya ungkit tinggi terhadap pencapaian SDGs sehingga target dalam menurunkan Angka Kematian Ibu (AKI) dapat tercapai. Kata kunci: bantuan operasional kesehatan, kesehatan ibu, cakupan, pelayanan

scholarly journals A Correlation Study of the Effectiveness of Teaching Practice between Taught Programs and Personal In-Built Performance Talents

2021 ◽  
Vol 3 (3) ◽  
pp. 41-50
Author(s):  
Rozhgar Jalal Khidhir
Keyword(s):  
Classroom Management ◽  
Linear Relationship ◽  
Teaching Practice ◽  
Pearson Correlation ◽  
Correlation Study ◽  
P Value ◽  
Pearson Correlation Test ◽  
Teaching Programs ◽  
The University ◽  
The Relationship

The study aims to identify the types of relationships related to the conversation, teaching methods, language testing, syntax, micro-teaching, classroom management, and teaching practice.  It is hypothesized that there is no linear relationship between the two variables, r= 0. In other words, no connection is there between the teaching programs at the university and performing well at schools during practising. Thus, and by contrast, there is a linear relationship between the two variables, r ≠0.  In other words, the teaching programs at the university and teaching practice are related. However, there is an uncertainty that performing well at schools could be solely due to the effectiveness of taught programs and personal in-built talents.  Specifically, the study tries to answer these questions: 1. Is there any relationship between the teaching programs and teaching well at schools during practising? If yes, how strong is that? 2. Which teaching program is relatively strongly related to the teaching practice module? And 3. Does personal in-built talent bring about an escalation in performing well at schools during practicing? Also, the study aims to figure out any connection between programs at university and teaching performance at schools by identifying the type and the magnitude of the relationship available. Also, it attempts to highlight the most influential positive or negative connection between the taught program variables. Further, it reveals the rationale beyond teaching practice achievements and, consequently, relates them to the actual causes. The study revealed that there is a positive relationship between all the selected modules and teaching practice based on the Pearson Correlation test calculating the coefficient value output at 0.494 with a p. value of 0.000. Since correlation does not mean causation, the findings report a kind of confusion about whether teaching programs are beyond teaching well at schools.

scholarly journals Hubungan Cakupan Tablet Fe3 dengan BBLBR di Provinsi Jawa Timur tahun 2017

2019 ◽  
Vol 3 (4) ◽  
pp. 291
Author(s):  
Irohatul A'ila ◽  
Sri Sumarmi
Keyword(s):  
Quantitative Research ◽  
Pearson Correlation ◽  
Secondary Data ◽  
Health Indicators ◽  
P Value ◽  
Health Profile ◽  
Chi Square ◽  
High Prevalence ◽  
Pearson Correlation Test ◽  
Chi Square Test

Background: Low birth weight (LBW) is one of the children's health indicators. Based on the health profile of East Java province in 2017, the prevalence of LBW increased from 3.6% in 2016 to 3.8%. Besides increasing the prevalence of LBW, there was a decrease in Fe3 tablet coverage. In 2016 Fe3 tablets decreased by 88.2% and decreased in 2017 to 87.4%. A decrease in Fe3 tablet coverage is likely to cause a high prevalence of LBW.Objective: This study aimed to know the relationship of Fe3 tablets with the prevalence of LBW in East Java province in 2017. Methods: The type of this research was quantitative research using secondary data on the health profile of East Java province in 2017. The observed variables was Fe3 tablet coverage and LBW prevalence. Data analysis using a Pearson correlation and Chi-square test.Result: The regency/city included in the category of low Fe3 tablet coverage was 55.3% and as many as 21.1% regency/city with LBW prevalence were included in the category of not public health problems. Pearson correlation test results (p-value=0.270) and Chi-square test (p-value=0.206) showed there was no relationship between the coverage of Fe3 tablets with prevalence of LBW in East Java Province in 2017.Conclusion: The regency/city that has low Fe3 tablet coverage doesn’t necessarily have a high LBW prevalence. Further research is needed to find out other factors related to the high prevalence of LBW in East Java Province in 2017.ABSTRAKLatar Belakang: Berat badan lahir rendah (BBLR) merupakan salah satu indikator kesehatan anak. Berdasar profil kesehatan Provinsi Jawa Timur tahun 2017, kejadian BBLR mengalami peningkatan dari 3,6% pada tahun 2016 menjadi 3,8%. Selain peningkatan prevalensi BBLR, terjadi penurunan cakupan tablet Fe3 (90 tablet Fe) yang diberikan kepada ibu hamil. Pada tahun 2016 cakupan tablet Fe3 sebesar 88,2% dan menurun pada tahun 2017 menjadi 87,4%. Cakupan pemberian tablet Fe kepada ibu hamil merupakan salah satu faktor yang dapat berhubungan dengan kejadian BBLR.Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan cakupan tablet Fe3 untuk ibu hamil dengan kejadian BBLR di Provinsi Jawa Timur pada tahun 2017.Metode: Jenis Penelitian ini adalah penelitian kuantitatif menggunakan data sekunder profil kesehatan Provinsi Jawa Timur tahun 2017. Data sekunder yang digunakan berupa data cakupan tablet Fe3 dan kejadian BBLR. Analisis data menggunakan uji korelasi perason.Hasil: Hasil uji korelasi pearson antara cakupan tablet Fe3 dengan kejadian BBLR di Provinsi Jawa Timur pada tahun 2017 menunjukkan nilai p-value <0,05 (p=0,000). Hal ini menunjukkan adanya hubungan antara cakupan tablet Fe3 dengan kejadian BBLR di Provinsi Jawa Timur tahun 2017 dengan koefisiensi korelasi r=0,706.Kesimpulan: erdapat hubungan antara cakupan tablet Fe3 dengan kejadian BBLR di Provinsi Jawa Timur pada tahun 2017.

scholarly journals PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Diana Chusna Mufida ◽  
Yuna Annisa Salsabila ◽  
Enny Suswati ◽  
Bagus Hermansyah ◽  
Dini Agustina
Keyword(s):  
Streptococcus Pneumoniae ◽  
Epithelial Cells ◽  
Immune Responses ◽  
Western Blotting ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Pearson Correlation ◽  
P Value ◽  
Sds Page ◽  
Pearson Correlation Test

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 

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