scholarly journals Antioxidant Activities of Heracleum platytaenium extracts and Essential Oil

2018 ◽  
Vol 6 (9) ◽  
pp. 1274
Author(s):  
Tunay Karan ◽  
Nusret Genc
Keyword(s):  
Essential Oil ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Moderate Activity ◽  
Etoac Extract ◽  
Scavenging Effect ◽  
Octyl Acetate ◽  
Dpph Scavenging

Heracleum platytaenium Boiss. has been used in traditional medicine. Antioxidant effect of essential oil as well as extracts of H. platytaenium was investigated. The essential oil was acquired by hydro distillation using a Clevenger type apparatus and GC-MS was used to analyze the essential oil compounds. Antioxidant capacity including ABTS+•, DPPH• scavenging and reducing power activity tests were carried out for essential oil and extracts. Moreover, total phenolic and total flavonoid contents were investigated. n-Octyl acetate (36.5%), apiole (24.9%), and elemicin (20.8%) were the chief products of essential oil. The essential oil and extracts exhibited from weak to moderate activity. The total phenol varied from 19.01 to 130.99 mg GAE/g extract and total flavonoid was fluctuated from 2.0 to 118.4 mg QE/g extract. The most DPPH• scavenging effect was observed in EtOAc extract (IC50 = 24.09 mg ml –1). The ABTS•+ scavenging effect of EtOAc extract was better than synthetic antioxidants BHA, BHT and Trolox.

Chemical Constituents Isolated from Rhododendron ungernii with Antioxidant Profile

2019 ◽  
Vol 9 (3) ◽  
pp. 238-243 ◽  
Author(s):  
Emine Dede ◽  
Nusret Genc ◽  
Mahfuz Elmastas ◽  
Huseyin Aksit ◽  
Ramazan Erenler
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Isolation And Identification ◽  
Reduced Pressure ◽  
Scavenging Effect ◽  
Pharmaceutical Industries ◽  
Dpph Scavenging

Background: Plant in Rhododendron genus that contains phenolic compounds has been used in traditional medicine and revealed considerable biological activities. Objective: Isolation and identification of antioxidant natural products from Rhododendron ungernii. Methods: Rhododendron ungernii Trautv. flowers were collected and dried in shade. The dried flowers were extracted with methanol for 3 days. The solvent was removed by reduced pressure to yield the extract which was subjected to column chromatography (Sephadex LH-20, C18 reversed phase column) to isolate catechin-7-O-glucoside (1), quercetin-3-O-β-galactoside (2), quercetin-3-O- β-xyloside (3), farrerol (4), myricetin (5), and quercetin (6). The structures of isolated compounds were elucidated by spectroscopic methods such as 1D-NMR, 2D-NMR, and LC-TOF/MS. DPPH scavenging effect, ABTS+ scavenging activity, and reducing power (FRAP) were performed for antioxidant assays of isolated natural compounds. Results: Isolated flavonoids displayed the outstanding antioxidant activities. Catechin-7-O-glucoside (1) and quercetin-3-O-β-galactoside (2) (IC50, 3.66 µg/mL) had the most DPPH• scavenging effect among the compounds. The highest ABTS•+ scavenging activity (IC50, 1.41 µg/mL) and reducing power effect (6.05 mmol TE/g comp) were observed for myricetin (5). Conclusion: R. ungernii extract and isolated compounds could be a promising antioxidant for food and pharmaceutical industries.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals Chemical Composition, Total Phenolic Content, and Antioxidant Activities of the Essential Oils of the Leaves and Fruit Pulp of Annona muricata L. (Soursop) from Ghana

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Joseph Nana Gyesi ◽  
Rita Opoku ◽  
Lawrence Sheringham Borquaye
Keyword(s):  
Hydrogen Peroxide ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Moderate Activity ◽  
Annona Muricata ◽  
Fruit Pulp ◽  
Leaf Essential Oil

Annona muricata, also called soursop, is widespread in many tropical countries, and various parts of the plant have been shown to possess very good pharmacological properties. This work evaluated the chemical composition and antioxidant activities of essential oils obtained from the fruit pulp and leaves of soursop. Essential oils were obtained via hydrodistillation and characterized by gas chromatography-mass spectrometry. Antioxidant potential was evaluated via the phosphomolybdenum, hydrogen peroxide scavenging, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays. In the leaf essential oil, a total of 31 compounds were identified with δ-cadinene (22.58%) and α-muurolene (10.64%) being the most abundant. Thirty-two compounds were identified in the fruit pulp essential oil with Ç-sitosterol (19.82%) and 2-hydroxy-1-(hydroxymethyl) ethyl ester (13.48%) being present in high amounts. Both essential oils showed very good total antioxidant capacities (49.03 gAAE/100 g and 50.88 gAAE/100 g for fruit pulp and leaf essential oils, respectively). The IC50 values from the DPPH assay were 244.8 ± 3.2 μg/mL for leaf essential oil and 512 ± 5.1 μg/mL for the fruit pulp essential oil. At 1 mg/mL, hydrogen peroxide scavenged was below 50% for both leaf and fruit pulp essential oils, indicating moderate activity. These results suggest possible application of the essential oils of Annona muricata in food preservation and processing.

Phenolic Content, Antibacterial and Antioxidant Activities of Moroccan propolis

2020 ◽  
Vol 15 (6) ◽  
pp. 696-705
Author(s):  
Omar Belmehdi ◽  
Badia Douhri ◽  
Abdelhakim Bouyahya ◽  
Yousif Laghmouchi ◽  
Nadia S. Senhaji ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Capacity ◽  
Bleaching Test ◽  
Ethanol Extracts ◽  
Dpph Scavenging

Background:Propolis contains compounds with antibacterial and antioxidant capacities, such as phenolic compounds and flavonoids. The aim of this study was to investigate the antibacterial and antioxidant effects of six propolis samples from northern Morocco and to determine their content of polyphenols and flavonoids.Methods:The total polyphenols and flavonoids of Ethanol Extracts (EEP) were evaluated by colorimetric assays. To test the antibacterial activity, the well diffusion and micro-dilution methods were adopted. The kinetic of bacterial growth in the presence of EEP was tested. The antioxidant activity was evaluated using three techniques: reducing power, β-carotene bleaching test, and DPPH scavenging capacity assay.Results:The EEPs were found to have high total phenolic content (65.09 - 122.00 mg GAE/g) and flavonoids (41.95 - 50.44 mg QE/g). The screening of the antibacterial activity showed that all tested bacteria were sensitive to EEP except Escherichia coli. The inhibitory zones varied from 11.50 ± 0.70 to 32.00 ± 0.70 mm, and MIC and MBC ranged from 0.07 to 5 mg/mL and 0.07 to >5 mg/mL, respectively. The antioxidant activity was proportional to the phenolic content of propolis. The IC50 varied from 48.70 to 156.00 mg/mL and the RAA% ranged from 75.07 to 96.68 %.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Antioxidant and antimicrobial activities of the essential oil and solvent extracts of Mentha pulegium L.

2015 ◽  
Vol 13 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Ivan Palic ◽  
Jovana Ickovski ◽  
Aleksandra Djordjevic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Mentha Pulegium ◽  
Antioxidant Power ◽  
Dry Extract

We report the total phenolic (TPC; expressed as gallic acid equivalents, GAE, per milligram of dry extract weight) and the total flavonoid contents (TFC; expressed as quercetin equivalents, QE, per milligram of dry extract weight) and antimicrobial and antioxidant activities of the essential oil and hexane, diethyl ether, ethyl acetate and methanol extracts of Mentha pulegium L. (Lamiaceae) collected in Serbia. The total phenolic content was in the range of 129.43-388.29 ?g GAE/mg, while TFC ranged from 57.81 to 160.94 QE/mg; the highest TPC and TFC were found in the methanol extract. The antimicrobial activity (against five bacteria and two fungi species) of the essential oil and solvent extracts was assessed using disc-diffusion method. However, the studied samples demonstrated a poor antimicrobial potential. The antioxidant activity was screened using five different tests: 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical cation decolorization assay (ABTS), total reducing power (TRP), ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant capacity assay (CUPRAC); the methanol extract showed the strongest antioxidant potential. The results of the different antioxidant assays were correlated mutually and with the total flavonoid and total phenolic contents (regression analysis and agglomerative hierarchical clustering).

scholarly journals A Study on Organic Tomatoes: Effect of a Biostimulator on Phytochemical and Antioxidant Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Varinder Sidhu ◽  
Dilip Nandwani ◽  
Li Wang ◽  
Ying Wu
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Treatment Groups ◽  
Significant Difference ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The objective of the present study is to investigate nutritional and antioxidant activity of four types of organic tomato cultivars. The differences in tomato quality are also tested between groups with or without treatment using an organic biostimulator, Stimplex. Total phenolic compounds (TPC), lycopene,β-carotene, DPPH free radical scavenging activity, reducing power, and color parameters were investigated in the current study. The results showed that there was no significant difference in TPC among cultivars regardless of Stimplex treatment. Higher lycopene andβ-carotene were obtained in Stimplex treated tomatoes. Lycopene andβ-carotene contents were significantly different among cultivars (P<0.05). DPPH scavenging activity in controlled group was significantly higher than that in the Stimplex treated tomatoes (P<0.05). No significant difference in reducing power was detected among cultivars treatment groups. The study showed that the darker the tomato color, the higher the lycopene andβ-carotene contents and the stronger the reducing power.

scholarly journals Antioxidant activity of rye caryopses and embryos extracts

2011 ◽  
Vol 20 (No. 6) ◽  
pp. 209-214 ◽  
Author(s):  
M. Karamać ◽  
R. Amarowicz ◽  
S. Weidner ◽  
S. Abe ◽  
F. Shahidi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Beta Carotene ◽  
Uv Spectra ◽  
Model System ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Scavenging Effect

Phenolic compounds were extracted with 80% methanol from caryopses and embryos of rye (cv. Dańkowskie Złote and Amilo). In all extracts, reducing power, scavenging effect on DPPH radical, and antioxidant activity in a &beta;-carotene-linoleate model system were examined. The highest content of total phenolic compounds was noted in the extract from caryopses of Amilo (7.93 mg/g of extract). UV spectra of all extracts were characterised by maxima originated from phenolic acids (320, 326 and 328 nm), and by maxima at shorter wavelengths (272 and 274 nm) attributed to other phenolic compounds. All extracts showed a good antioxidant activity in a &beta;-carotene-linoleate model system. This activity was similar to that reported before in leguminous seeds extracts. The antioxidant activities of the extracts from the caryopses of Dańkowskie Złote and the embryos of both cultivars were very similar, especially during the second part of the incubation period. The extract of Amilo embryos showed a slightly weaker antioxidative effect. The weak antiradical effects in the experiments with DPPH radical and a weak reducing power were characteristic for all the extracts investigated. &nbsp;

scholarly journals Effects of Storage Time and Temperature on Antioxidants in Juice from Momordica charantia L. and Momordica charantia L. var. abbreviata Ser.

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3614
Author(s):  
Yung-Sheng Lin ◽  
Wen-Ying Huang ◽  
Pang-Yen Ho ◽  
Shiou-Yih Hu ◽  
Ying-Yi Lin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Storage Time ◽  
Storage Temperature ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Dry Weight ◽  
Momordica Charantia ◽  
Total Phenolic ◽  
High Storage Temperature ◽  
Dpph Scavenging

This study determined the antioxidant activities of juice from Momordica charantia L. (MC) and MC var. abbreviata Ser. (MCVAS) by analyzing 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing power (FRP), and total phenolic content (TPC). The effects of storage time and storage temperature on these antioxidant activities were investigated. Liquid chromatography–mass spectrometry was conducted to identify the major components of MC and MCVAS. The results revealed that the antioxidant activity of MCVAS was better than that of MC, possibly because of richer components of MCVAS. For MC and MCVAS, the scavenging concentrations of 50% DPPH were 3.33 and 1.19 mg/mL, respectively; moreover, the FRP values were 68.93 and 118.14 mg ascorbic acid equivalent/g dry weight, respectively; and the TPC values were 8.15 and 11.47 mg gallic acid equivalent/g dry weight, respectively. The antioxidant activities of MC and MCVAS decreased with storage time. High storage temperature decreased antioxidant activity more quickly than a low temperature. In addition, MC had exhibited a faster decline in DPPH scavenging ability and FRP than MCVAS during 24-day storage, but no difference was observed in TPC.

scholarly journals Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

2021 ◽  
Vol 9 (6) ◽  
pp. 1124-1128
Author(s):  
Fatma Ergün
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phenolic Substance ◽  
Amount Of Substance ◽  
Phenolic And Flavonoid

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

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