scholarly journals Antibacterial and Antifungal Activity of Clove Extract (Syzygium Aromaticum): Review

2021 ◽  
Vol 2 (2) ◽  
pp. 93-103
Author(s):  
Nathaniel Hiwandika ◽  
Susana Elya Sudrajat ◽  
Ika Rahayu

Cloves is one of the native Indonesian plants and is used in many aspects of life. Cloves are used in multiple industries and as detergents, soaps, perfumes, food seasonings, aromatherapy, etc. Cloves are mainly used for ingredients in kretek cigarettes in Indonesia. Various studies of cloves reported they have good pharmacological and therapeutic effects. The main compounds of clove extract are eugenol and β-caryophyllene, which are powerful antibacterial and antifungal agents. The clove ethanolic extract showed the activity to inhibit Gram positive and negative bacteria such as B. cereus, S. aureus, E. coli, P. aeruginosa, S. pneumoniae, S. aureus, S. epidermidis, A. hydrophila, K. pneumoniae, P. gingivalis, and P. mirabilis. Clove essential oil has shown the ability to inhibit the growth of V. inaequalis, C. albicans, C. glabrata, and C. tropicalis. Cloves extract can be used as an essential ingredient of various medicines. However, it requires further research and trials.

Author(s):  
Laila Jarin ◽  
Md Shafiqur Rahman ◽  
MN Anwar

Petroleum ether, carbon tetrachloride, chloroform and ethyl acetate extracts of Plumeria rubra leaves were studied for their antimicrobial activities against eleven human pathogenic bacteria, viz., Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae and four human pathogenic fungi, viz., Aspergillus niger, A. ochraceus, A. ustus and Candida albicans using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extract exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extracts exhibited the largest zone of inhibition (25 mm in diameter with 2000 mg/disc extract) against E. coli. The highest inhibition of fungal radial mycelial growth (62.00% with 100 mg extract/ml medium) was recorded against A. ustus with ethyl acetate extract. The MICs were determined by broth macrodilution technique. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against E. coli. However, for fungi the lowest MIC was 500 mg/ml against A. ustus with the same extract. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13409 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):87-94, 2008


2011 ◽  
Vol 8 (s1) ◽  
pp. S149-S154 ◽  
Author(s):  
P. Panneerselvam ◽  
G. Geete Ganesh

The syntheses of series of 2, 5-disubstituted 1, 3, 4-oxadiazole derivatives are described. A total of twelve new compounds were synthesized and characterized by IR,1H-NMR and Mass spectral data. All newly synthesized compounds were screened for their antimicrobial activityi.e. antibacterial activity againstS. aureusandE. coliand antifungal activity against fungusA. nigar. CompoundsG5andG7exhibited significant both antibacterial and antifungal activity whileG2,G10andG3,G9showed antibacterial and antifungal activity respectively. These compounds were 2, 5-disubstituted 1, 3, 4-oxadiazole moiety at position two and five showed reasonable antibacterial and antifungal activity.


Author(s):  
Neeraj Kumar ◽  
Dhruti Bhatt ◽  
Chandra Shekhar Sharma ◽  
Hamendra Pratap Singh ◽  
Harshda Pandiya ◽  
...  

A series of of Chalconyl Incorporated Schiff’s Bases of Sulphonamides was synthesized by reacting substituted chalcone derivatives with Sulphacetamide sodium in methanol. All the title compounds synthesized (2a-2d) were tested for antibacterial and antifungal activity using E. coli, P. aeruginosa, S. aureus, S. pyogenus and C. albicans, A. niger and A. clavatus respectively as microbial strains and Sulphacetamide sodium as standard. The compound 2d showed significant antibacterial activity and 2a showed moderate antifungal activity as compared with Sulphacetamide Sodium.


2003 ◽  
Vol 58 (9-10) ◽  
pp. 687-690 ◽  
Author(s):  
Ahmet C. Goren ◽  
Gokhan Bilsel ◽  
Mine Bilsel ◽  
Huseyin Demir ◽  
E. Esin Kocabaş

AbstractThe water-distilled essential oil the leaves of Coridothymus capitatus were analyzed by GC/MS and also analyzed by direct thermal desorption GC/MS. Comparison was made between two analyses techniques. The essential oil consisted mainly of monoterpenes 98.9%, while oxygenated hydrocarbons were identified as 55.6% and non-oxygenated hydrocarbons as 43.6%. As major components were found carvacrol (35.6%), p-cymene (21.0%), thymol (18.6%), γ-terpinene (12.3%), α-terpinene (3.2%), β-myrcene (3.0%) and α-thujene (1.3%) by hydrodistillation and by the GC/MS method. The direct thermal desorption GC/MS analysis also showed the same major components, namely carvacrol (51.6%), thymol (21.7%), pcymene (9.7%) γ-terpinene (8.2%), α-terpinene (1.64%). The essential oil of C. capitatus showed strong activity against S. aureus, P. vulgaris, P. aeruginosa, E. coli, K. pneumonia, B. subtilis, E. faecalis, S. epidermidis and C. albicans.


2021 ◽  
pp. 3048-3055
Author(s):  
Tiurma Pasaribu ◽  
Arnold P. Sinurat ◽  
Elizabeth Wina ◽  
Triwardhani Cahyaningsih

Background and Aim: Many plants contain bioactive substances with antibacterial and antifungal properties. The aim of this study was to evaluate the antibacterial and antifungal activity of Cocos nucifera shell liquid smoke (CSL), clove leaf extract (CLE), and mangosteen pericarp extract (MPE) alone and in combination against Escherichia coli and Candida utilis. The antioxidant activity, phenol, saponin, and tannin of CSL, CLE, MPE, and Phyllanthus niruri L. extract were also measured. Materials and Methods: The agar well-diffusion method was used to determine the antimicrobial and antifungal activities of CSL, methanolic MPE, and CLE and their combination CSL+MPE+CLE (COMBI) on bacteria E. coli and fungus (C. utilis). Antioxidant activity was measured by the diphenylpicrylhydrazyl method. Total phenol and total tannin were measured by the Folin–Ciocalteu method and total saponin was measured by the vanillin-sulphate method. Results: The results indicated that phenolic and tannin levels were greater in MPE than in CLE, whereas the saponin content was higher in CLE compared with MPE. Undiluted (100%) MPE exhibited lower antibacterial activity (p<0.05) than chloramphenicol against E. coli, however, undiluted CLE and COMBI showed similar activity compared with chloramphenicol against E. coli. COMBI caused significantly (p<0.05) higher inhibition compared with virginiamycin against E. coli. CSL, MPE, and COMBI exhibited significantly lower antifungal activity (p<0.05) than that of ketoconazole against C. utilis. In contrast, CLE showed improved antifungal activity (p<0.05) compared with ketoconazole. Conclusion: Cocos nucifera liquid smoke, Garcinia mangostana pericarp extract, and Syzygium aromaticum leaf extract, either alone or in combination, have the potential to be used as antibacterial and antifungal agents.


2021 ◽  
Vol 7 (2) ◽  
pp. 79
Author(s):  
Isnaini Isnaini ◽  
Agung Biworo ◽  
Husnul Khatimah ◽  
Khusnan Mustofa Gufron ◽  
Shafa Rahmani Puteri

Galam (Melaleuca cajuputi subsp. Cumingiana (Turcz.) Barlow) is a peat swamp forest plant. This plant is used to treat respiratory diseases, colitis, sore throat, itchy skin, diarrhea, and dizziness. This study aimed to analyze the antibacterial and antifungal activity of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit against Escherichia coli and Candida albicans bacteria. This research is a pretest-posttest research with control group design and consists of 3 stages of research. The first stage is the phytochemical screening test, the second stage determines the minimum inhibitory concentration (MIC) and the third determines the minimum bactericidal concentration (MBC). The results showed that the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flowers and fruit contained terpenoids, flavonoids, phenols, saponins, quinones, steroids, tannins, while alkaloid compounds only found in the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit. The MIC and MBC values ​​of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower methanol extract against E. coli were 1.67 and 2.083 mg/mL, respectively, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow flower against C. albicans were 3.125 and 25 mg/mL. The MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against E. coli have same value  were 3.334 mg/mL, while the MIC and MBC values ​​of the methanol extract of M. cajuputi subsp. Cumingiana (Turcz.) Barlow fruit against C. albicans were 3.125 and 12.5 mg/mL, respectively. Keywords: Galam  antibacterial, antifungal, Escherichia coli, Candida albicans


2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
José Lourenço dos Santos Cunha E Silva ◽  
Bruna de Paula Bicudo ◽  
Allan Belarmino Rodrigues ◽  
Milena Mariano Mendonça ◽  
Rodrigo Raghiant Borges ◽  
...  

2023 ◽  
Vol 83 ◽  
Author(s):  
B. Javed ◽  
F. Farooq ◽  
M. Ibrahim ◽  
H. A. B. Abbas ◽  
H. Jawwad ◽  
...  

Abstract The present study was aimed to manifest the antibacterial and antifungal activity of methanolic extracts of Salix alba L. against seven Gram-positive and Gram-negative bacterial pathogens e.g. Streptococcus pyogenes, Staphylococcus aureus (1), S. aureus (2), Shigella sonnei, Escherichia coli (1), E. coli (2) and Neisseria gonorrhoeae and three fungal isolates from the air such as Aspergillus terreus, A. ornatus, and Rhizopus stolonifer. Two different serotypes of S. aureus and E. coli were used. The agar well-diffusion method results showed the dose-dependent response of plant extracts against bacterial and fungal strains while some organisms were found resistant e.g. E. coli (1), S. sonnei, A. terreus and R. stolonifer. The highest antibacterial activity was recorded at 17.000±1.732 mm from 100 mg/mL of leaves methanolic extracts against S. pyogenes while the activity of most of the pathogens decreased after 24 h of incubation. The highest antifungal activity was reported at 11.833±1.0 mm against A. ornatus at 50 mg/mL after 48 h of the incubation period. These experimental findings endorse the use of S. alba in ethnopharmacological formulations and suggest the use of methanolic extracts of the said plant to develop drugs to control the proliferation of resistant disease causing pathogenic microbes.


Author(s):  
Amita Shobha Rao ◽  
Shobha Kl ◽  
Manjunath S Shetty ◽  
Sreedhara R Pai K

Objective: The present study focuses on in vitro antimicrobial properties of aqueous and ethanol leaf extract of Acacia auriculiformis tested on Gram-positive cocci, Gram-negative bacilli, multidrug-resistant (MDR) Gram-negative bacilli, and fungus.Methods: Ethanol and aqueous extracts of the leaves of A. auriculiformis were prepared. Agar well diffusion was the method for antimicrobial susceptibility. Freshly grown standard strains of Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae (K. pneumoniae) , Escherchia coli (E.coli) and Pseudomonas aeruginosa, clinical strains of Streptococcus pneumoniae, Candida albicans (C. ailbicans), and MDR E. coli, and MDR Klebsiella pnuemoniae were used. Ampicillin disc (10 μg) was used as control.Results: The zone of inhibition was measured to determine the antimicrobial activity. Ethanolic extract of A. auriculiformis exhibited antibacterial activity against all the strains including MDR strains of K. pneumoniae and E. coli. Antifungal activity was exhibited by both aqueous and ethanol leaf extracts of A. auriculiformis.Conclusion: Ethanol extract could be used against MDR K. pneumoniae and MDR E. coli. Similarly, aqueous and ethanol extract can be the drug of choice for C. albicans infection. Further study is necessary to evaluate the accurate compound responsible for antibacterial and antifungal activity for pharmaceutical applications.


Author(s):  
Firza Shafique ◽  
Uzma Naureen ◽  
Annam Zikrea ◽  
Sohail Akhter ◽  
Tanzila Rafique ◽  
...  

Spinach (Spinacia oleracea L. SO) leaves represent an important dietary source, have high nutritional value and antimicrobial properties. Spinach leaves have been used in the treatment of human diseases since ancient times. Here, the aim of this study was to evaluate the antimicrobial and antifungal activities of ethanolic extract of Spinach oleracea leaves by determining the minimum inhibitory concentrations (MICs) using well diffusion method against bacterial        species Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus and Klebseilla pneumonia and fungal species Aspergillus fumigatus, Aspergillus niger,        Candida albicans and Fusarium oxysporum. We evaluated from present data that ethanolic extract of root showed zone of inhibition ranges from 6 mm to 21 mm, ethanolic extract of stem showed zone of inhibition ranges from 8 mm to 21 mm and ethanolic extract of leaf showed zone of inhibition ranges from 9 mm to 22 mm from concentration 25 mg/ml to 100mg/ml. Leaf extract has high antibacterial and antifungal activity against bacterial and fungal species while root extract has low antibacterial and antifungal activity against bacterial and fungal species. Activity of plant extract was increased by the increasing concentration of extracts. Very low zone of inhibition was found at concentration 25 mg/1 ml DMSO which ranges from 6 mm to 14 mm while very high zone of inhibition was found at concentration 100 mg/ml which ranges from 6 mm to 22 mm. So, ethanolic extracts of Spinach oleraceae has good efficiency against bacterial and fungal species.


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