scholarly journals Pola Kepekaan Bakteri Penyebab Pneumonia terhadap Antibiotika di Laboratorium Mikrobiologi RSUP Dr. M. Djamil Padang Periode Januari sampai Desember 2011

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Jaka Kurniawan ◽  
Erly Erly ◽  
Rima Semiarty
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Research Method ◽  
Antibiotic Sensitivity ◽  
Secondary Data ◽  
Empiric Therapy ◽  
Test Results ◽  
Microbiology Laboratory ◽  
Sensitivity Pattern ◽  
Antibiotic Sensitivity Test

AbstrakPeningkatan resistensi bakteri penyebab pneumonia terhadap beberapa antibiotika yang lazim digunakan oleh klinisi dalam pemberian terapi empirik akan menyebabkan berkurangnya keefektifan terhadap terapi pneumonia. Hal ini akan berdampak semakin tingginya morbiditas dan mortalitas yang disebabkan oleh infeksi pneumonia. Tujuan penelitian ini adalah untuk mengetahui pola kepekaan bakteri penyebab pneumonia terhadap antibiotika di Laboratorium Mikrobiologi RSUP. Dr. M. Djamil Padang periode Januari 2011 - Desember 2011. Metode penelitian yang digunakan adalah deskriptif retrospektif terhadap data sekunder hasil uji kepekaan antibiotika dan jenis kuman dari 432 pasien, menunjukkan jenis bakteri penyebab pneumonia adalah Klebsiella sp, Streptococcus α haemolyticus, Staphylococcus aureus, Pseudomonas aeuruginosa, proteus sp, Basillus, Escherichia coli dan Enterobacter. Pola kepekaannya menunjukkan bahwa rata-rata semua bakteri tersebut mempunyai sensitivitas tertinggi terhadap Netilmicin, Meropenem, dan Sulbactam + Cefoperazone. Resistensi tertinggi ditunjukkan oleh Erithromycin, Ampicillin, Sulfamethroxazole + Trimethroprime,Tetracyclin, dan Choramphenicol.Kata kunci: antibiotika, bakteri penyebab pneumonia, pola kepekaan AbstractIncreased resistance of bacteria that cause pneumonia to some antibiotics that are commonly used by clinicians in the provision of empiric therapy, it will cause a reduction in the effectiveness of the treatment of pneumonia. This will lead to increasing morbidity and mortality caused by pneumonia infection. The objective of this study wass to determine the sensitivity pattern of bacteria that cause pneumonia to antibiotics in the Department of Microbiology Laboratory. Dr. M. Djamil Hospital Padang period from January 2011 to December 2011. The research method used was a descriptive and retrospective review of secondary data and antibiotic sensitivity test results of 432 patients types of germs. The results indicated the type of bacteria that cause pneumonia are Klebsiella sp, Streptococcus α haemolyticus, Staphylococcus aureus, Pseudomonas aeuruginosa, Proteus sp, Basillus, Escherichia coli, and Enterobacter. Sensitivity pattern showed that the average of all the bacteria has the highest sensitivity to Netilmicin, Meropenem, and Sulbactam + Cefoperazone, Highest resistance shown by Erithromycin, Ampicillin, Sulfamethroxazole Trimethroprime +, Tetracycline, and Choramphenicol.Keywords: antibiotics, the bacteria that caused pneumonia, sensitivity pattern

scholarly journals Bacteria Sensitivity Pattern of Caesarean Section Wound on Selected Antibiotics in Ulin General Hospital Banjarmasin

2020 ◽  
Vol 16 (2) ◽  
pp. 73
Author(s):  
Nurhafizah Rafiani ◽  
Renny Aditya ◽  
Noor Muthmainah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Caesarean Section ◽  
Staphylococcus Epidermidis ◽  
Antibiotic Sensitivity ◽  
Cross Sectional ◽  
Surgical Wounds ◽  
Sensitivity Pattern ◽  
Antibiotic Sensitivity Test ◽  
Bacterial Sensitivity

Abstract: A cesarean delivery increases the risk of wound infection it should be prevented by using antibiotics. This study was to determine the pattern of bacterial sensitivity in surgical wounds of cesarean section patients for selected antibiotics, i.e ceftriaxone, cefixime, sulbactam ampicillin, ciprofloxacin, clindamycin and gentamicin.This observational study was conducted at the Ulin Public Hospital in Banjarmasin from July to September 2019. Using a cross sectional approach Samples of bacteria were identidied from 36 that were pusposively sampled, i.e., Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli. Antibiotic sensitivity test showed that Staphylococcus aureus was sensitive against gentamicin (100%),whereas Staphylococcus epidermidis and Escherichia coli. Were intermediately sensitive towards gentamicin (62.5%) and ceftriaxone (80%) Keywords: Antibiotic susceptibility, caesarean section, surgical wound

scholarly journals Pola Kepekaan Bakteri terhadap Antibiotik di Ruang Rawat Intensif RSPI Prof. Dr. Sulianti Saroso Jakarta

2017 ◽  
Vol 1 (2) ◽  
pp. 23
Author(s):  
Putriana Indah Lestari ◽  
Ika Susanti ◽  
Huda Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Antibiotic Sensitivity ◽  
Secondary Data ◽  
Sensitivity Pattern ◽  
Important Health ◽  
Antibiotic Sensitivity Test ◽  
Antibiotics Sensitivity

Abstrak : Penyakit infeksi merupakan salah satu masalah kesehatan yang penting. Penggunaan antibiotik yang tidak rasional dan tepat guna pada pasien penyakit infeksi beresiko menyebabkan terjadinya resistensi. Tujuan dari penelitian ini yaitu untuk mengetahui pola kepekaan bakteri terhadap antibiotik pada pasien Ruang Rawat Intensif (ICU) RSPI Prof. Dr. Sulianti Saroso (RSPI-SS) Jakarta. Penelitian dilakukan deskriptif dan retrospektif terhadap data sekunder hasil uji kepekaan antibiotik dan jenis bakteri dari 107 pasien dalam kurun waktu 2011. Hasil menunjukkan 68 (65,4%) pasien mendapatkan hasil kultur positif dan uji kepekaan bakteri terhadap antibiotik. Jenis bakteri patogen yang dominan yaitu Acinetobacter baumannii (29,4%), disusul oleh Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) dan Escherichia coli (8,8%). Sebagian besar bakteri pada pasien ICU RSPISS telah berkurang kepekaannya (resisten) terhadap beberapa antibiotik. A. baumannii dan P. aeruginosa merupakan bakteri yang paling resisten terhadap antibiotik uji. Pola kepekaannya menunjukkan bahwa bakteri patogen mempunyai resistensi tertinggi terhadap erythromycin dan terendah terhadap amikasin.Infectious diseases is an important health problem. Irrational antibiotics usage is a leading cause in initiating drugs resistances. A preliminary study was conducted on the sensitivity pattern of microorganisms against antibiotics at the intensive care unit of Sulianti Infectious Diseases Hospital Jakarta. Retrospective. Secondary data were collected on the results of antibiotics sensitivity tests and species of microorganisms of 107 patients during the year 2011. Sixty eight (65,4%) patients were positive on microorganism culture test and tested on antibiotic sensitivity test. Predominance pathogenic species found were Acinetobacter baumannii (29,4%), followed by Pseudomonas aeruginosa (27,9%), Klebsiella pneumoniae (13,2%) and Escherichia coli (8,8%). Most species were less sensitive (resistant) to several antibiotics. The pattern of sensitivity showed that pathogenic microorganisms were the most resistant against erythromycin and the most sensitive antibiotics was amikacin.

Antibiotic Sensitivity Pattern of Bacterial Isolates from Different Clinical Specimens: Experience at NICVD, Dhaka

2012 ◽  
Vol 5 (1) ◽  
pp. 67-72 ◽  
Author(s):  
MS Shahidullah ◽  
MA Yusuf ◽  
Z Khatun ◽  
UKMN Ara ◽  
MT Mitul
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Antibiotic Sensitivity ◽  
Number Of Patients ◽  
Sensitivity Pattern ◽  
Pseudomonas Species ◽  
Severe Infections ◽  
Medical Wards ◽  
And Staphylococcus Aureus

Background: Infectious diseases are among the leading causes of death and sometimes curable.  Bacteria are the most common etiology in hospitalized patients. The objective of this study was to  evaluate the incidence of bacterial infections and their pattern of susceptibility to antibiotics in  moderate and severe infections in patients admitted at NICVD hospital.Methodology: The study was performed in the apical teaching hospital of Bangladesh situated at  Dhaka in the first half of 2012. Patients admitted in medical wards and medical ICU, suffering from  moderate and severe infections were studied. Clinical evaluation, routine and specific investigations  were done in each case. Microbiological samplings were tried on day 1, after completion of antibiotic  therapy or in between as required. Aerobic bacterial culture and sensitivity tests were done.  Result: A total of 274 cases were studied of which male (67.5%) were predominant than female  (32.5%). The highest number of patients were in the age group of 30-60 years (53.6%) followed by 10-  30 years (24.5%) and more than 60 years (12.8%). The mean age with standard deviation was  39.15±19.07 years (range 1-90 years). Most common isolated bacteria was the Pseudomonas species  (14.2%) followed by Escherichia coli (13.5%), Staphylococcus saprophyticus (6.9%) and Staphylococcus  aureus (2.2%). From these specimens Pseudomonas species (31.5%) was isolated mostly from pus. E.  coli was found most commonly in pus (16.3%) and urine (14.1%). Staph saprophyticus (13.0%) and  Staphylococcus aureus (4.3%) were both found most commonly in pus. Pseudomonas species was  100% resistant to Penicillin, Amoxycillin and Vancomycin. It was found that Pseudomonas species  was still more than 90% sensitive only to Imipenem. Escherichia coli was more than 80% sensitive to  only Imipenem and Amikacin. Staphylococcus aureus was sensitive to only Imipenem and Cephalexin.  Conclusion: In this study Pseudomonas species and Escherichia coli are the most common isolated  bacteria in this Institution. Most of the antibiotics are resistant to these two bacteria. DOI: http://dx.doi.org/10.3329/cardio.v5i1.12276 Cardiovasc. j. 2012; 5(1): 67-72

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

scholarly journals Bacteriological Profile and Antibiotic Sensitivity Pattern in Infected Diabetic Foot Ulcers

Esculapio ◽  
2020 ◽  
Vol 16 (03, july 2020-Septmber 2020) ◽  
Author(s):  
Afshan Zia
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Antibiotic Sensitivity ◽  
Diabetic Foot Ulcer ◽  
Empiric Therapy ◽  
Sensitivity Pattern ◽  
Pseudomonas Species ◽  
Third Generation Cephalosporins ◽  
Proteus Species

Objectives: To identify the common bacterial pathogens responsible for infection in diabetic foot ulcer and their sensitivity pattern to different antibiotics. Methods: This prospective observational study was conducted from 24th June 2019 to 27th December 2019 at Services Institute of Medical Sciences Lahore. Specimens of discharge from diabetic foot ulcers (DFU) were received from Diabetes Management Centre (DMC). Cultures were put up and bacteria isolated were identified by standard methods. Antibiotic sensitivity was determined by Kirby-Bauer disc diffusion method. Results: Samples from DFU of 50 patients were processed. Thirty-six (72%) samples were from males and 14(28%) were from females; mean age of patients was 53±9.5 years. Forty-nine (98%) patients had unsatisfactory glycemic control. Forty three (86%) samples were growth positive while 07(14%) were bacteriologically sterile. Monomicrobial infection was observed in 38 (76%) cases while polymicrobial infection was seen in 12 cases (24%). The most common isolates were Staphylococcus aureus 14(28%), Pseudomonas species 11 (22%) and Proteus species 10 (20%). Forty three percent of Staphylococcus aureus, were methicillin resistant (MRSA). All MRSA remained sensitive to vancomycin and linezolid. In Pseudomonas species, resistance to third generation cephalosporins, ceftazidime , was 27%, while resistance to imipenem was seen in 3 (9%) of isolates. In Proteus species, resistance to third generation cephalosporins was 90% while to imipenem resistance was 60%. Resistance to commonly prescribed quinolones was more than 70% among all the bacterial isolates. Conclusions: Common Gram positive and Gram negative organisms responsible for infection in DFU were Staphylococcus aureus, Pseudomonas species and Proteus species. The isolates were multi-drug resistant (MDR). Resistance to antibiotics used as empiric therapy was high. Keywords: Diabetic foot ulcer, antibiotic resistance, empiric therapy

scholarly journals Isolation and Identification and Antibiotics resistance of some Pathogenic bacteria from ants

2019 ◽  
Vol 17 (72) ◽  
pp. 55-60
Author(s):  
Sawsan Hassan Authman ◽  
Nibras Nazar Mahmood ◽  
Zaid Ra'ad Abas
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
External Surface ◽  
Antibiotic Sensitivity ◽  
Antibiotics Resistance ◽  
Isolation And Identification ◽  
Antibiotic Sensitivity Test ◽  
Internal Component ◽  
Klebsiella Spp

This study was carried out on 60 ants samples collected from different places included: Gardens House, Food, died Cockroaches and W.C. Two methods were used: Isolation of bacteria from external surface and internal component, that to show the importance of this insect and their role in transferring of pathogenic bacteria. The results showed isolation of 30 isolates of different bacterial genus at 50%, included Staphylococcus aureus at highest ratio 26.23% then Escherichia coli, Bacillus subtilis, Klebsiella spp., Proteus spp., Pseudomonas areuginosa and Sarcinia at (23.34, 13.34, 13.34, 10, 6.67, 6.67)% respectively. The study revealed that the ants ply an important role in transferring these microbes. The majority of that isolates were on their external surface 63.33% in comparison to that isolated from internal component. The antibiotic sensitivity test for isolates against (7) antibiotics were resistance to multi antibiotics specially Vancomycin, whilst most isolates were sensitive to Nitrofurantin.

scholarly journals Evaluation of Bacterial Species in Patients with Skin Infection and their Antibiogram

2021 ◽  
pp. 10-16
Author(s):  
Immaculata U. Nwankwo ◽  
Kelechi C. Edward ◽  
Chinedu N. Nwoba ◽  
Chinyere V. Okwudiri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Skin Infection ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Percentage Occurrence ◽  
Antibiotic Sensitivity Test ◽  
Microbiological Criteria

Introduction: The skin is a barrier that limits invasion and growth of pathogenic bacteria. Bacterial species in patients with skin infection and their antibiogram were evaluated for the presence of inhabiting bacteria. Method: The sample obtained with swab sticks were streaked on their respective culture plates containing nutrient agar, MacConkey agar and blood agar, incubated 370 for 24h. The recovered bacteria after incubation were characterized and identified according to standard microbiological criteria. Results: Five bacteria species were isolated which includes Staphylococus aureus; Escherichia coli, Klebsiella specie, Proteus sp and Pseudomonas aeruginosa. Staphylococus aureus (33.03%) had the highest percentage occurrence while Proteus Sp (9.17%) had the lowest percentage occurrence. The highest number of isolates were recovered from male patients (61.47%). Age wise the least percentage of isolates were gotten from age group 31-40 (1.83%) while the highest was obtained from those within the age range of 11-20 (40.37%) followed by 1-10 years of age (25.69%). More isolates were recovered from in-patients (68.81%) than from Out-patients (31.19%). The antibiotic sensitivity test showed that Klebsiella sp, Proteus spp and Staphylococcus aureus were sensitivity to all the antibiotics tested while pseudomonas aeruginosa had 30% activity (sensitivity to only 3 drugs) for the antibiotics used in this study. Conclusion: From this study, it can be said that Staphylococcus aureus, Escherichia coli, Klebsiella sp, Proteus sp and Pseudomonas aeruginosa are implicated in the skin infections in humans and their presence if not treated will lead to long term disease in human affected.

scholarly journals Microbiological Profile of Severe Lower Respiratory Tract Infection in Intensive Care Unit of a Tertiary Care Center of Dhaka, Bangladesh

2015 ◽  
Vol 2 (2) ◽  
pp. 53-56
Author(s):  
M Abdul Mannan ◽  
M Abul Kashem ◽  
Fazle Rabbi Mohammed ◽  
Raihan Rabbani ◽  
M Motiul Islam
Keyword(s):  
Escherichia Coli ◽  
Intensive Care Unit ◽  
Staphylococcus Aureus ◽  
Intensive Care ◽  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
Sensitivity Pattern

Background: Microbial resistance to antibiotics is nowadays a great threat to intensive care unit patients especially in severe pneumonia leading to high morbidity and mortality. Bacterial profile and antibiotic sensitivity pattern would help clinician in selecting more proper empirical therapy before isolation of microbes.Materials and Methods: This retrospective study was performed among 210 patients of lower respiratory tract infection in intensive care unit in Square Hospitals Ltd, a tertiary care hospital in Dhaka. Sputum, tracheal aspirates or broncho alveolar lavage were cultured, identified and antibiotic sensitivity pattern performed by standard methods.Results: Of 210 specimens, 150 (71%) were culture positive and 60 (29%) showed no growth. From 150 culture positives, 216 isolates were recovered, of which 7 specimen shows more than 2 organisms each, 52 specimen shows 2 isolates per specimen remaining 91 specimen with single isolates were recovered. The most common organisms in order of frequency were, Acinetobacter baumannii (24.0%), Staphylococcus aureus (20.6%), Klebsiella pneumoniae (19.3%), Pseudomonas aeruginisa (19.3%), Escherichia coli (14%) and Candida albicans (13%). A very high rate (83-93%) of resistance was observed among Acinetobactor baumanni to Beta lactum and clavulanate, Cephalosporin, Quinolones and Carbapenem. No resistant was observed with Colistin. Linezolid and Vancomycin are most effective against methicillin resistant Staphylococcus aureus, Coagulase negative Staphylococcus aureus and Escherichia coli, where no resistance was found.Conclusion: Farmenter and nonfermenters are the both common etiological agents of lower respiratory tract infections in this intensive care unit. There is high rate of resistance to commonly used cephalosporin and ?-lactam-?-lactamase inhibitors and quinolone group of drugs. Acinetobacter baumannii, Pseudomonas aeruginisa and Klebsiella showed most sensitivity to Colistin and actually no resistance was found.Bangladesh Crit Care J September 2014; 2 (2): 53-56

scholarly journals Bacteriophage-derived CHAP domain protein, P128, kills Staphylococcus cells by cleaving interpeptide cross-bridge of peptidoglycan

Microbiology ◽  
2014 ◽  
Vol 160 (10) ◽  
pp. 2157-2169 ◽  
Author(s):  
Sudarson Sundarrajan ◽  
Junjappa Raghupatil ◽  
Aradhana Vipra ◽  
Nagalakshmi Narasimhaswamy ◽  
Sanjeev Saravanan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mechanism Of Action ◽  
Catalytic Domain ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Common Mechanism ◽  
Cross Bridge ◽  
Sensitivity Pattern

P128 is an anti-staphylococcal protein consisting of the Staphylococcus aureus phage-K-derived tail-associated muralytic enzyme (TAME) catalytic domain (Lys16) fused with the cell-wall-binding SH3b domain of lysostaphin. In order to understand the mechanism of action and emergence of resistance to P128, we isolated mutants of Staphylococcus spp., including meticillin-resistant Staphylococcus aureus (MRSA), resistant to P128. In addition to P128, the mutants also showed resistance to Lys16, the catalytic domain of P128. The mutants showed loss of fitness as shown by reduced rate of growth in vitro. One of the mutants tested was found to show reduced virulence in animal models of S. aureus septicaemia suggesting loss of fitness in vivo as well. Analysis of the antibiotic sensitivity pattern showed that the mutants derived from MRSA strains had become sensitive to meticillin and other β-lactams. Interestingly, the mutant cells were resistant to the lytic action of phage K, although the phage was able to adsorb to these cells. Sequencing of the femA gene of three P128-resistant mutants showed either a truncation or deletion in femA, suggesting that improper cross-bridge formation in S. aureus could be causing resistance to P128. Using glutathione S-transferase (GST) fusion peptides as substrates it was found that both P128 and Lys16 were capable of cleaving a pentaglycine sequence, suggesting that P128 might be killing S. aureus by cleaving the pentaglycine cross-bridge of peptidoglycan. Moreover, peptides corresponding to the reported cross-bridge of Staphylococcus haemolyticus (GGSGG, AGSGG), which were not cleaved by lysostaphin, were cleaved efficiently by P128. This was also reflected in high sensitivity of S. haemolyticus to P128. This showed that in spite of sharing a common mechanism of action with lysostaphin, P128 has unique properties, which allow it to act on certain lysostaphin-resistant Staphylococcus strains.

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