STUDY OF PARSLEY ESSENTIAL OIL

Author(s):  
Т.В. ПЕЛИПЕНКО ◽  
О.Н. СПОДА ◽  
С.К. МУСТАФАЕВ ◽  
В.Е. КРЕПАК ◽  
Э.Э. КОЧИЕВА

Исследованы количественные и качественные изменения эфирного масла из травянистого сырья петрушки огородной (Petroselinum sativum) в фазе технической спелости растения – в период от молочной до молочно-восковой спелости плодов центральных зонтиков. Установлено, что выход эфирного масла увеличен на 50%. Определено, что прирост общего содержания эфирного масла влечет увеличение выхода большинства его компонентов. Наибольший прирост наблюдается для апиола (300%), массовая доля которого достигает 7,8%, что необходимо учитывать при организации уборки и в технологии переработки травянистого сырья P. sativum. Также существенно возрастает содержание в масле 1,3,8-пара-ментатриена – до 28,30%, обусловливающего аромат зелени петрушки. Более 70% общего количества компонентов масла составляют углеводороды и среди них, помимо 1,3,8-пара-ментатриена, альфа- и бета-пинены, лимонен. Рекомендовано проводить уборку травянистого сырья петрушки на стадии молочно-восковой спелости плодов центральных зонтиков. Доказана целесообразность дальнейших исследований содержания эфирного масла в различных органах растения на разных этапах фазы технической спелости для более полной характеристики количественных и качественных изменений и разработки технологии комплексной переработки травянистого сырья петрушки. The study of quantitative and qualitative changes of parsley essential oil Petroselinum sativum based on herbal raw materials in a phase of the technical ripeness of a plant – in the period from milk-ripe stage to milky-waxy stage of fruit ripeness of central parsley umbels allowed us to find out that at this stage the output of essential oils grows by 50%. There has been a steady increase in the total content of essential oils which leads to the increase in the output of most of its components. The biggest growth is observed for parsley camphor (300%), which mass fraction is 7,8%. It is necessary to take this fact into consideration during the harvest season and in the processing of parsley herbal raw materials P. sativum. It should be mentioned that hydrocarbons constitute 70% from the total amount of essential oil components, among them are 1,3,8-p-menthatriene, as well as alpha- and beta-pinenes, limonene. On the base of carried out research it is recommended to harvest parsley herbal raw materials at the milky-waxy stage of fruit ripeness of central parsley umbels. It is expedient to study changes in essential oils of various plants’ organs at various stages of technical ripeness for more complete characteristics of qualitative and quantitative changes and the development of complex technology of processing of parsley herbal raw materials.

2020 ◽  
pp. 39-53
Author(s):  
Tatyana Shcheglova

The article presents results of the study of a comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage (Salvia officinalis L.), growing in the Botanical Garden of the First Moscow State Medical University named after Sechenov. The studies were carried out using gas chromatography– mass spectrometry. Qualitative and quantitative difference in the component composition of the essential oil obtained from fresh and dried raw materials was determined as a result of the study.


Author(s):  
Mansureh Ghavam ◽  
Afsaneh Afzali ◽  
Maria Manconi ◽  
Gianluigi Bacchetta ◽  
Maria Letizia Manca

Abstract Background Essential oil of Rosa × damascena Herrm. is one of the most valuable and important raw materials for the flavor and fragrance industry. The cultivation of this plant has ancient origins, and Kashan was one of the first mountainous regions of Iran dealing with the cultivation of R. × damascena. In this study, both chemical composition and antimicrobial activity of different rose essential oils obtained from five mountainous areas of Kashan region (Maragh, Qamsar, Sadeh, Javinan, and Kamoo) has been investigated along with the influence of the environmental conditions on these properties. Results Results showed that yield and chemical composition of essential oils obtained from Rosa × damascena were significantly affected by the collection area. In particular, the yield of oils varied from ~0.08 to ~0.132% and citronellol (36.70-9.18%), geraniol (12.82-0.47%), nonadecane (22.73-10.36%), heneicosane (31.7-11.43%), and 1-nonadecene (6.03-3.93%) have been detected as main compounds in all the plants collected, but at different concentrations depending on the collection area. The best fragrance and the highest yield were found in the oil from Kamoo area. Similarly to the chemical composition, the antimicrobial activity of the essential oils was affected by their origin, and essential oil obtained from plants collected from Kamoo area disclosed the highest antibacterial and antifungal efficacy. Its inhibition halos were 17.33±0.58 mm against Aspergillus brasiliensis, 15.67±0.58 mm against Staphylococcus aureus, and 12.33±0. 58 mm against Streptococcus pyogenes. Essential oils of R. damascena were also effective against Gram-negative Pseudomonas aeruginosa and they had a MIC value of 62.50 μg/mL irrespective of the collection area (except the oil from Javinan area). On the contrary, the highest antifungal power against Candida albicans yeast was reached using the essential oil obtained from plants collected in Javinan region (MIC and MBC ~62.50 μg/mL). Conclusions Overall results underline the influence of environmental conditions of the different areas of Kashan region, on the chemical composition of and antimicrobial activity of the essential oils of Rosa × damascena. In addition, results disclosed that Kamoo seemed to be the most suitable area for the competitive cultivation of R. × damascena to the intensive production of aromatic flower oil and natural antimicrobial essential oils.


2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Ewa Skała ◽  
Patrícia Rijo ◽  
Catarina Garcia ◽  
Przemysław Sitarek ◽  
Danuta Kalemba ◽  
...  

The essential oils were isolated by hydrodistillation from the hairy roots (HR) and roots of soil-grown plants (SGR) of Rhaponticum carthamoides and were analyzed by GC-MS method. In the both essential oils 62 compounds were identified. The root essential oils showed the differences in the qualitative and quantitative composition. The sesquiterpene hydrocarbons (55–62%) dominated in both essential oils. The major compounds of HR essential oil were cyperene, 13-norcypera-1(5),11(12)-diene, and cadalene while aplotaxene, nardosina-1(10),11-diene, and dauca-4(11),8-diene dominated in SGR essential oil. Both essential oils showed antibacterial activity especially against Enterococcus faecalis (ATCC 29212) and Pseudomonas aeruginosa (ATCC 27853) (MIC value = 125 µg/mL). HR and SGR essential oils also decreased the expression of IL-1β, IL-6, and TNF-α and the ROS level in LPS-treatment astrocytes. This is the first report to describe the chemical composition of R. carthamoides essential oil from hairy roots, its protective effect against LPS-induced inflammation and ROS production in astrocytes, and its antimicrobial potential. The results show that R. carthamoides hairy roots may be a valuable source of the essential oil and may be an alternative to the roots of soil-grown plants.


Author(s):  
Zuriah Ismail ◽  
Azhari Hamid Nour ◽  
Salah Elhussein

Different accessions of basil introduced to Malaysia as seeds and cultivated at University Malaysia Pahang (UMP) farm and their essential oils extracted by steam distillation. Three chemotypes of these essential oils with estragole, linalool and methyl cinnamate as dominant compounds were evaluated against three facultative anaerobic bacteria obtained from the National Pharmaceutical Control Bureau, Ministry of Health Malaysia: namely, Escherichia coli, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) by qualitative and quantitative method. Different concentration ranged from 10 μL/mL to 1000 μL/mL of basil essential oil solutions prepared and tested against bacterial strain using agar well diffusion and quantitative methods. Minimum inhibitory concentration (MIC) was performed using broth microdilution plate. Eight different concentrations of serial two-fold dilutions ranged between 250 μL/mL and 1.95 μL/mL performed using Magellan software of Tecan Infinite Series M200 Pro microplate reader. In the result of agar well diffusion test, the zone of inhibition increased as the concentration of essential oil increased. The MIC was 7.81 μL/mL for all chemotype of the oils against E.coli and S.aureus while 15.63 μL/mL for P.aeruginosa of linalool-rich chemotype and 31.25 μL/mL for estragole and methyl cinnamate-rich chemotypes. The results obtained in this study were considered encouraging the potential of basil essential oil for medicinal uses as antibiotics and hygienic purposes as antibacterial products.


Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 909 ◽  
Author(s):  
Olga Kosakowska ◽  
Katarzyna Bączek ◽  
Jarosław L. Przybył ◽  
Anna Pawełczak ◽  
Katarzyna Rolewska ◽  
...  

Common thyme is regarded as one of the most important culinary plants. The purpose of the work was to determine the intraspecific variability of common thyme with respect to morphological and chemical characters including the content and composition of essential oil and phenolic compounds in the herb. The objects of the study were 12 clones, vegetatively multiplied from randomly selected individual plants of cultivar ‘Standard Winter’. The morphological observations and harvest of raw materials were carried out in the first year plants’ vegetation. The highest differences between clones were on fresh and dry weight of herb (CV = 0.38 and 0.36, respectively), width of leaves (CV = 0.21), and density of glandular trichomes on the abaxial surface of leaves (CV = 0.29). Examined clones were also differentiated as to the chemical features. Essential oil content (performed by hydrodestillation) and composition (by GC-MS and GC-FID) were determined and they ranged from 2.10 to 4.38 g × 100 g−1 DW. Here, thymol, γ-terpinen, and p-cymen were the dominant compounds. Clone no. 4 was distinctive as to the highest content of essential oil followed by the highest share of thymol (54.59%). The total content of phenolic acids and flavonoids (determined according to PPh 6th) also differed among clones (CV = 0.38 and 0.36, respectively). Using a validated HPLC-DAD method, the following compounds were identified: caffeic, rosmarinic, p-coumaric acids, luteolin 7-O-glucoside, naryngenin, and (−)-epicatechin. Here, rosmarinic acid followed by luteolin 7-O-glucoside were present in the highest amounts (611.47–2675.59 and 46.77–325.11 mg × 100 g−1 DW, respectively). The highest differences between clones were the contents of p-coumaric acid (CV = 0.59), luteolin 7-O-glucoside (CV = 0.50) and rosmarinic acid (CV = 0.40). Such a high range of variability can provide problems with raw material standardization. Nevertheless, it opens possibilities for breeders, whereas individual plants/clones may become valuable components for breeding.


2018 ◽  
Vol 1 (1) ◽  
pp. 5
Author(s):  
Malse Anggia ◽  
Sri Mutiar ◽  
Dewi Arziah

Essential oils are used as raw materials in various industries. Plants containing essential oils are flower kenanga and citronella fragrance. This study aims to create prototype of essential oil distillation equipment. Essential oils obtained from the prototype, then tested as an aroma therapy in liquid soap. The result of oil refining has been done several experiments by using citronella fragrance and kenanga flower. Device productivity goes well. This is indicated by the oil obtained from lemongrass and kenanga oil in the process of distillation. The resulting volatile oil is applied to the manufacture of liquid soap. Yield oil yields were 0.22%. and the fragrant lemongrass obtained is 0.12%. Based on the organoletic test on the resulting soap product, the average of the color is 3.7 (likes), the viscosity is 3.55 (likes), the aroma is 3.05 (regular) and the 3.55 foam (likes) on the kenanga aroma soy while the average color is 4.05 (like) viscosity 3.1 (regular), aroma 3.05 (regular) and foam 3.45 (likes) on liquid soap the aroma of citronella perfume. Keywords : Essential oil; Extraction; Lemongrass;  Soap


2020 ◽  
Vol 24 ◽  
pp. 00029
Author(s):  
Akzhunis Imanbayeva ◽  
Meruert Sagyndykova ◽  
Margarita Ishmuratova

For the first time, the dynamics of the accumulation of essential oil in the underground organs of Ferula foetida grown on the territory of the Mangistau region (the Western Kazakhstan), depending on the age of the plant and the place of growth, are investigated. The maximum accumulation of essential oil is noted for the 4th year of vegetation (0.98%), the minimum – for the annual plants (0.14%). The largest quantitative composition of essential oil is noted in the sands of Tuyesu and on the elevation of Tynymbay Shoky, the minimum – in the vicinity of the Karkol wintering. As the results of the study the optimal places of raw materials harvesting and collection dates are determined.


2019 ◽  
pp. 173-180
Author(s):  
Юрий (Yuriy) Алексеевич (Alekseevich) Морозов (Morozov) ◽  
Ифрат (Ifrat) Назимович (Nazimovich) Зилфикаров (Zilfikarov) ◽  
Елизавета (Elizaveta) Владимировна (Vladimirovna) Морозова (Morozova) ◽  
Аслан (Aslan) Мурадалиевич (Muradalievich) Алиев (Aliev) ◽  
Тимур (Timur) Алгасанович (Algasanovich) Ибрагимов (Ibragimov)

This paper presents the results of an experimental study of the component composition of essential oil samples obtained from various vegetable raw materials of Schisandra chinensis (Schisandra chinensis (Turcz.) Baill.): branches (woody stems), rhizomes with roots and leaves. The extraction of essential oils was carried out by distillation with water vapor followed by extraction from the distillate with ethoxyethane. The largest yield of essential oil in terms of completely dry raw material is registered from rhizomes with roots – 0.99%; the yield from leaves and branches was approximately the same: 0.51% and 0.52%, respectively. A comparative analysis of the component composition of the obtained essential oils was carried out using the gas chromatography / mass spectrometry method. In the essential oil of the leaves 29 detected compounds (identified by 21 connection; dominant components: Cyclohexylmethyl ether sulphurous acid, (+)-trans-nerolidol, δ-cadinene, Tridec-(2E)-EN-1-ol), woody stems 80 connections (identified 65 compounds; dominant components: ß-pinene, Camphene, Bornylacetate, (+)-trans-nerolidol, p-tsimen, Tridecan-2-it, δ-cadinene), rhizomes with roots 78 of the compounds (60 compounds identified; dominant components: δ-cadinene, Bornylacetate, β-pinene, Camphene, Nerolidol-(E), Tridecan-2-it, γ-cadinene, Borneol). For each sample of the studied essential oils selectivity and marker substances were established.


2021 ◽  
Vol 285 ◽  
pp. 01007
Author(s):  
R.R. Timirgaleeva ◽  
M.V. Verdysh ◽  
A.A. Popova ◽  
N.Yu. Polyakova

This article reveals that essential oil production is a complex system with a large number of economic ties arising in the process of growing and processing as well as distribution of products made from essential oils raw materials. Multileveled manufacturing process of essential oils products requires coordination of all the elements to reduce costs in the entire supply chain. It is proposed to use mathematical modeling of the supply chain based on the method of main faces, which allows to take into account the specifics of the problem and makes it possible to use a variety of combinatorial and heuristic algorithms.


Author(s):  
I.V. Belova ◽  
◽  
E.N. Grunina ◽  
N.V. Glumova ◽  
◽  
...  

A complex technology for processing raw rosemary is proposed. This technological scheme allows making maximum use of the phytopotential of Rosmarinus officinalis L. raw materials and expanding the range of essential oil products. As a result of complex processing, it is possible to obtain not only rosemary essential oil but also such additional products as natural fragrant water, aqueous and alcoholic extracts of rosemary, etc. These products will allow the processing company to gain extra income and will be in demand in various industries.


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