STUDY OF PROTEIN AND FATTY ACID COMPOSITION OF LUPINE SEEDS OF DIFFERENT SPECIES

Author(s):  
М.Л. ДОМОРОЩЕНКОВА ◽  
Т.Ф. ДЕМЬЯНЕНКО ◽  
И.М. КАМЫШЕВА ◽  
И.В. КРЫЛОВА

Исследования фракционного состава белков и жирнокислотного состава масел безалкалоидных и малоалкалоидных сортов люпина для производства кормов и продуктов питания являются актуальными в современных социально-экономических условиях, когда требуется найти альтернативу соевым продуктам. В данной работе изучались фракционный состав белков и жирнокислотный состав масла семян 11 образцов малоалкалоидных сортов люпина отечественной селекции трех видов: белого, желтого и узколистного. Самое высокое содержание сырого протеина выявлено в образцах семян желтого люпина, в семенах белого люпина содержание сырого протеина было немного ниже, а все исследованные образцы сортов узколистного люпина характеризовались самым низким содержанием сырого протеина. Суммарное количество растворимых белков в семенах люпина было высоким и изменялось от 84,21 до 92,45 %. Во всех исследованных образцах сортов белого люпина преобладала альбуминовая фракция, в семенах сортов узколистного и желтого люпина – глобулиновая фракция. Содержание сырого жира в семенах разных сортов изменялось от 4,62 до 9,28%. Обнаружены различия в жирнокислотном составе масла семян люпина разной видовой принадлежности. Жирнокислотный состав семян характеризовался преобладанием олеиновой кислоты в масле семян сортов белого люпина, при явном преобладании линолевой кислоты в сортах желтого и узколистного люпина. Researches of protein fractional composition and fatty-acid composition of nonalkaloid and low-alkaloid lupine varieties oils for food production are actual in modern social and economic conditions, when it is required to find an alternative for soybean products. In the present work, the protein fractional composition and fatty acid composition of seeds of 11 low-alkaloid lupine varieties of domestic selection of three species: white, yellow and narrow-leaved lupine were investigated. The highest content of crude protein was detected in seed samples of yellow lupine, the crude protein content in white lupine seeds was slightly lower, and all the studied samples of narrow-leaved lupine were characterized by the lowest crude protein content. The total amount of soluble proteins in lupine seeds was high and varied from 84,21 to 92,45 %. In all examined samples of white lupine varieties the albumin fraction dominated, while in seeds of narrow-leaved and yellow lupine varieties the globulin fraction dominated. The crude fat content in the seeds of the different varieties varied from 4,62 to 9,28%. Differences were found in the fatty acid composition of lupin seed oil of different species. The fatty-acid composition of seeds was characterized by the predominance of oleic acid in the oil of white lupine varieties, with a clear predominance of linoleic acid in yellow and narrow-leaved lupine varieties.

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184279 ◽  
Author(s):  
Shahla Hosseini Bai ◽  
Ian Darby ◽  
Tio Nevenimo ◽  
Godfrey Hannet ◽  
Dalsie Hannet ◽  
...  

2017 ◽  
Vol 71 (2) ◽  
pp. 111-118
Author(s):  
Radoslav Sevic ◽  
Dragomir Lukac ◽  
Vitomir Vidivic ◽  
Nikola Puvaca ◽  
Bozidar Savic ◽  
...  

The aim of this study was to provide a comparative analysis of chemical and fatty acid composition, as well as of the connective tissue proteins in pigs of different genotypes, Mangalitsa and Landrace. Both pig genotypes were fed with the same feed of standard composition and quality. At the end of the fattening period, in total 24 pigs of both genotypes were slaughtered. Based on the analysis of the chemical composition we came to the conclusion that the protein content in both genotypes was similar. Moisture and ash content in the Landrace pig genotype differed significantly (P < 0.01) compared with genotype of Mangalitsa breed. Statistically significant differences (P < 0.01) were established in the fat content, which was 7.95 g/100 g, in pigs of Mangalitsa breed and 1.59 g/100 g in the Landrace pigs breed. Content of hydroxyproline, non-proteinogenic amino acids, in meat of Landrace was significantly higher (P < 0.01) compared to the content in the Mangalitsa breed. The same tendency was observed with regard to the connective tissue protein content, as well as with the relative connective tissue protein content. The fatty acid composition of the meat indicated that the most common saturated fatty acid (SFA) in both tested breeds was palmitic fatty acid (C16), whose content was significantly higher in Landrace (P < 0.01) compared with its content in Mangalitsa breed. In addition, the share of stearic acid (C18) was significantly higher (P < 0.01) in Landrace compared to Mangalitsa pig breed, what significantly contributed to the increase of the SFA share in Landrace compared to Mangalitsa breed. The most common monounsaturated fatty acid in both pig breeds was the oleic fatty acid (C18:1), whose share was significantly higher in Mangalitsa compared to the Landrace breed (P < 0.01). Out of the polyunsaturated fatty acids (PUFA), linoleic fatty acid (C18:2) was the most predominant in both pig breeds, with no statistically significant differences (P > 0.05). The content of PUFA was not statistically significantly different between the tested breeds, as well as the content of n-3 and n-6 fatty acids, which caused no statistically significant differences in the n-6/n-3 PUFA ratio. Ratio of the unsaturated fatty acids, i.e., of the sum of MUFA and PUFA, and of the saturated fatty acids was significantly higher in Mangalitsa compared to Landrace breed (1.86 vs. 1.4), and the same was observed when it comes to the relationship MUFA/SFA (1.51 in Mangalitsa vs. 1.08 in Landrace breed) and MUFA/PUFA (4.35 vs. 3.38).


2013 ◽  
Vol 58 (No. 7) ◽  
pp. 304-312 ◽  
Author(s):  
M. Johansson ◽  
O. Placha ◽  
J. Pickova ◽  
A. Andrén ◽  
G. Zamaratskaia ◽  
...  

Two concentrates, one protein-rich and one based on cereals, were combined with two silages with a crude protein content of 17 and 13% of dry matter (DM), respectively to give four different diets for dairy cows. Milk content of caseins (&alpha;<sub>S1</sub>-, &alpha;<sub>S2</sub>-, &beta;-, and &kappa;-casein) and whey proteins (&alpha;-lactalbumin (&alpha;-LA) and &beta;-lactoglobulin (&beta;-LG)) and the fatty acid profile of milk were analyzed before the start and on four occasions during the experiment. Milk analyses showed that diet had no influence on the protein profile of the milk. However, a significant increase of &alpha;-linolenic acid, 13 and 39%, was obtained on the high protein concentrate feed and on the silage higher in crude protein, respectively. Cows on the protein-rich concentrate diet increased the proportion of conjugated linoleic acid by 53%. Linoleic acid was not affected by the diet. &nbsp;


1979 ◽  
Vol 59 (3) ◽  
pp. 819-830 ◽  
Author(s):  
W. DIEPENBROCK ◽  
G. GEISLER

Plants of rape (Brassica napus L. ’Erglu’) were grown under controlled greenhouse conditions. The growth and composition of pods (hulls) and seeds was followed from the day when flowering took place to maturity. Pod development could be divided into three stages. In the first stage hull growth was more rapid than seed growth. Nitrogen accumulation in hulls occurred at a maximum and seeds were characterized by a high protein content but a low oil content and a fatty acid composition similar to that of photosynthetic tissue. During the second phase dry matter of hulls began to decrease, nitrogen was exported and the rates of seed dry matter accumulation grew to a maximum. At the same time the protein content of seeds declined while oil content increased rapidly. The fatty acid composition had reached the final condition. In the third stage hulls continued to lose dry matter and the rates of seed dry matter accumulation decreased dramatically. Pod growth was affected by the location of the pod on the plant. The individual seed weight and the fatty acid compvsition at 16 days after flowering indicated an accelerated development of seeds from axillary branches. Pods from the main branch generally produced the largest amount of seed dry matter. From these results the ideal high-yielding rape plant was characterized.


2007 ◽  
Vol 55 (1) ◽  
pp. 99-114 ◽  
Author(s):  
Z. Izsáki

In a long-term mineral fertilisation experiment with 64 treatments representing all possible combinations of four rates each of N, P and K, set up on chernozem meadow soil in Szarvas in 1989, the protein and oil contents and the amino acid and fatty acid compositions of the maize grain yield were analysed between 1997 and 2004.The protein content of the maize kernels increased by 1.1–1.5 percentage points up to a NO 3 -N level of 80–100 kg ha −1 in the 0–60 cm soil layer prior to sowing. The year had a greater influence on the protein content than the N supplies. No consistent effect of N on the amino acid composition, detectable as a change in the ratio of any amino acid in the majority of experimental years, was observed. In the AL-P 2 O 5 range of 120–362 mg kg −1 in the ploughed layer, the soil P supplies had no statistically significant effect on the kernel protein content. In most years the P supplies had little effect on the amino acid composition of the protein. The oil content and fatty acid composition of maize kernels was extremely stable, and was very little affected by the nutrient supplies or the year. During the experimental period excessive N supplies were only found to reduce the oil content and modify the fatty acid composition on one occasion. The oil content and fatty acid composition were not substantially affected by the P supplies.


1962 ◽  
Vol 59 (1) ◽  
pp. 93-94 ◽  
Author(s):  
T. Moran ◽  
J. Pace

The composition and feeding value of the common heather has been extensively studied by Thomas et al. (1934, 1935, 1953, 1955, 1956). He has shown that as the heather ages the protein content declines. Thus, samples from 2-year-old growths had a crude protein content (on a dry-matter basis) of 11·9% while that of growths 8 years old was 6·5–6·9%. Little or no information, however, appears to be available on the quality of heather protein, as judged by its amino acid composition.


2020 ◽  
Vol 50 (2) ◽  
pp. 222-231
Author(s):  
Anna Mukhortova ◽  
Olga Uzbekova ◽  
Ivan Lyzhov

Introduction. Deep-sea fauna of the North Atlantic has a great potential for the development of food industry. Rabbitfish (Chimaera monstrosa) is a hydrobionts that makes up a significant proportion of bycatch in the traditional trawl and longline fishery. The research objective was to determine the technochemical composition and biochemical properties of organs and tissues of rabbitfish. Study objects and methods. The size-mass and total chemical composition was performed by standard methods. The amino acid composition of proteins was determined by chromatographic separation of amino acid derivatives obtained by reaction with orthophthalic aldehyde and β-mercaptoethanol. The method of one-dimensional thin-layer chromatography made it possible to determine the fractional composition of lipids. The fatty acid composition was determined using an S-180 gas-liquid chromatograph (Yanaco, Japan). The fat-soluble vitamins were determined by the saponification of samples with alkali, extraction, and separation of the unsaponifiable part. The fractional composition of proteins became clear after a planar polyacrylamide gel electrophoresis on (MultiPhor II, Sweden). Results and discussion. The research featured the size-mass and chemical composition of body parts, the fractional and amino acid composition of proteins, as well as the fractional and fatty acid composition of lipids, vitamins, heavy metals, and organochlorine compounds in the tissues and organs of the rabbitfish. The sensory properties of the samples proved quite high. Hot smoked rabbitfish meat can be recommended for snack foods. Waste (heads, entrails, skin, cartilage, fins) makes up more than 50% of total body weight and can be used in feed production or as a protein-containing raw material for hydrolysates in microbiology, medicine, and food industry. Conclusion. The research involved a complex biochemical study of the rabbitfish organs and tissues. The performed technochemical studies made it possible to give preliminary recommendations on the directions of its most rational integrated use.


2021 ◽  
Vol 3 (27) ◽  
pp. 155-163
Author(s):  
O.P. Ptashnik ◽  

Due to the lack of irrigation, the question of replacing soybean with an equivalent crop in the context of nutritional value and adaptation to the weather conditions of Crimea arises. One of such crops can be white lupine (Lupinus albus L.). The purpose of the research was twofold: assess L. albus productivity and crude protein content in grain; identify the most promising varieties and breeding numbers of white lupine under conditions of the steppe Crimea. In 2016-2018, the research was carried out on the experimental field of the Research Institute of Agriculture of Crimea according to the B.A. Dospekhov methods of field research and methodology of the State variety testing of agricultural crops. We studied the influence of bioclimatic conditions of the steppe Crimea on the growth, development, grain yield formation and protein content of four varieties (‘Michurinsky’, ‘Alyi Parus’ (standard), ‘Dega’ and ‘Desnyansky 2’) and four promising breeding numbers (‘CH-1022-09’, ‘CH-1677-10’, ‘CH-1397-10’ and ‘CH-6-11’). A wide diversity of weather conditions during the years of research made it possible to evaluate the samples in favorable and stressful conditions. In drier years, the growing season lasted 93–97 days because of the reduction in interphase periods; in milder ones it was101 days. The results in grain yield have been mixed: from 0.48 to 1.45 t/ha in 2016; from 0.35 to 0.56 t/ha in 2017; from 0.39 to 0.54 t/ha in 2018. On average, over a three-year study, breeding numbers ‘CH-1022-09’ and ‘CH-1677-10’ were the most productive. Their yield was 0.65 and 0.81 t/ha, respectively. In 2017, crude protein content varied from 24.02 to 28.49%; in 2018 – from 26.05 to 31.1%. On average, over the years of study, the highest protein content was typical for the varieties ‘Alyi Parus’ (29.30%) and ‘Michurinsky’ (29.03%). Breeding numbers ‘CH-1022-09’ and ‘CH-1677-10’ were distinguished by the highest indicators of productivity and yield; in terms of crude protein content, varieties ‘Alyi Parus’ and ‘Michurinsky’ were the best. Consequently, they are the ones that are recommended for cultivation.


2008 ◽  
Vol 13 (1-2) ◽  
pp. 198 ◽  
Author(s):  
A-M. KIRKKARI ◽  
P. PELTONEN-SAINIO ◽  
P. LEHTINEN

Harvested naked oat is not completely hulless. Hull content of various cultivars ranged between one and six percent. Genotype and environment control expression of nakedness. Using different threshing settings at different grain moisture contents, it was investigated whether grain moisture at threshing and combine harvester settings affected hull content and its relationship to germination capacity. Naked groats were stored at room temperature and analysed for protein content and fatty acid composition to determine storability. Grain moisture content at threshing had contrary effects on hull content and degree of hull retention in different years. Small grains tended to retain hulls more tightly during threshing. Grain filling capacity appears to be the dominant factor determining degree of nakedness rather than stage of maturity. The postulated protective nature of hulls was confirmed only for cultivar Lisbeth. Highly viable samples of grain of cv. Lisbeth, threshed at normal settings, contained a higher percentage of hulls than those with low germination capacity, while for cv. Bullion, a protective effect of the hulls was not evident. Grain moisture content at threshing did not affect protein content of naked cultivars, but some differences in fatty acid composition were recorded. Changes in lipid composition and volatile oxidation products during storage of groats were relatively moderate, indicating no major problems related to storage when naked oat was dried well.;


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