Evaluation and Assessment of Efficient Psidium Guajava and Mentha Piperita Fresh Leaves Juice for Perio Pathogens

The research was assessed to evaluate the efficacy of fresh juice of Psidium guajava and Mentha piperita against selected aerobic and anaerobic bacterial strain. The fresh juice was extracted and evaluated for its antimicrobial activity on anaerobic pathogens like Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi). Antimicrobial activity was evaluated by the disc diffusion method and minimum inhibitory concentration. Long before since from the ancient time, humankind depended on the trees and herbs for medicines to alleviate ailments, search for better health, fragrance, flavours and food. In ancient time human beings depended on animals and plants for his food, shelter demand etc. Over 5000 years, peoples of India and China kept on the use of plants for food as well as to protect from disease. Most of the dental problems are due to microorganism (Oral Pathogens) like Fusobacterium, Actinomyces, Porphyromonas, Prevotella, Eubacterium, Bacteroids, Staphylococcus, Streptococcus, Enterococcus etc. Microbial debridement might uproot pathogenic living being due to their area. Previously, subepithelial gingival tissues, crevicular epithelial cells, other anatolian dialect features entangling sufficient defiant. The results indicate that Psidium guajava and Mentha piperita leaves shows significant Antimicrobial activity due to the presence of flavonoids. This work is more useful in health Mentha Piperita Fresh Leaves Juice for Perio Pathogens.

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ANTIMICROBIAL ACTIVITY OF SOME THAI AROMATIC PLANTS AGAINST ORAL PATHOGENS INDUCING HALITOSIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.28404 ◽

2019 ◽

Vol 12 (1) ◽

pp. 465 ◽

Cited By ~ 1

Author(s):

Plernpit Yasin ◽

Phenphicha Wanachantararuk ◽

Jidaphatinoi Jidaphatinoi ◽

Kanchana Dumri

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Agents ◽

Fusobacterium Nucleatum ◽

Diffusion Method ◽

Piper Betle ◽

Aromatic Plants ◽

Disk Diffusion Method ◽

Strong Activity ◽

Potential Source ◽

Oral Pathogens

Objective: The objective of the present work is to analyze that the extracts of 25 Thai aromatic plants used in Thai food recipes were subjected to evaluated antimicrobial activity against Fusobacterium nucleatum and Streptococcus mutans which have been known as bacterial inducing halitosis.Methods: Disk diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) by broth microdilution were used to determine the antibacterial activity.Results: The results revealed that three plant extracts, namely Piper betle Linn., Eupatorium stoechadosmum Hance, and Alpinia galangal (L.) Wild can inhibit the growth of both halitosis inducing bacteria with strong activity. Among these, the extract of P. betle Linn. is the most effective in inhibiting both bacteria followed by E. stoechadosmum Hance and A. galangal (L.) Wild, respectively. The MIC (mg/ml) values of the extracts which can inhibit F. nucleatum were ranging from 1.56 to 12.50, and the MBCs (mg/ml) values were ranging from 3.125 to 25.00. In addition, the MICs and MBCs of the extracts which can inhibit S. mutans were found that 1.56–25.00 and 3.125–50.00, respectively.Conclusion: The extracts obtained from P. betle, A. galangal, and E. stoechadosmum displayed good activity against F. nucleatum and S. mutans and these extracts could be a potential source of new antimicrobial agents.

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ANTIMICROBIAL ACTIVITY OF SOME THAI AROMATIC PLANTS AGAINST ORAL PATHOGENS INDUCING HALITOSIS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.28404 ◽

2019 ◽

Vol 12 (1) ◽

pp. 465

Author(s):

Plernpit Yasin ◽

Phenphicha Wanachantararuk ◽

Jidaphatinoi Jidaphatinoi ◽

Kanchana Dumri

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Agents ◽

Fusobacterium Nucleatum ◽

Diffusion Method ◽

Piper Betle ◽

Aromatic Plants ◽

Disk Diffusion Method ◽

Strong Activity ◽

Potential Source ◽

Oral Pathogens

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An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽

10.3390/ma13194383 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4383

Author(s):

Barbara Lapinska ◽

Aleksandra Szram ◽

Beata Zarzycka ◽

Janina Grzegorczyk ◽

Louis Hardan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Antimicrobial Agents ◽

Resin Composite ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Diffusion Method ◽

Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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Antimicrobial activity of Guava tree (Psidium Guajava) leaf extract and selected Commercial Antibiotics on Bacterial isolates from Kisubi Hospital

10.51168/sjhrafrica.v1i12.1. ◽

2020 ◽

Author(s):

◽

David Serunjogi ◽

Kizito Muwonge

Keyword(s):

Antimicrobial Activity ◽

Psidium Guajava ◽

Diffusion Method ◽

Resistant Bacteria ◽

Drug Resistant ◽

Leaf Extracts ◽

Drug Resistant Bacteria ◽

The World ◽

Guava Leaf ◽

Tree Leaf

Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.

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Antimicrobial effect of natural kinds of toothpaste on oral pathogenic bacteria

The Journal of Infection in Developing Countries ◽

10.3855/jidc.14966 ◽

2021 ◽

Vol 15 (10) ◽

pp. 1436-1442

Author(s):

Serdar Demir ◽

Gul Keskin ◽

Nese Akal ◽

Yasemin Zer

Keyword(s):

Antimicrobial Activity ◽

Adverse Effects ◽

Pathogenic Bacteria ◽

Natural Kinds ◽

Aloe Vera ◽

Antimicrobial Effect ◽

Diffusion Method ◽

Oral Pathogens ◽

Inhibition Zones ◽

Agar Well Diffusion Method

Introduction: Because of the adverse effects on human health of some antimicrobial ingredients in traditional toothpaste, consumers are increasingly turning to toothpastes with natural ingredients. This study evaluates the antimicrobial effect of toothpastes containing different natural active agents against three oral pathogens: Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis. Methodology: This study tested one traditional toothpaste and seven different natural toothpastes containing theobromine, aloe vera, miswak, propolis, chitosan, enzymes and probiotics. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analyzed. Results: Toothpastes containing theobromine and chitosan and the traditional toothpaste showed antimicrobial efficacy for all tested bacteria. Toothpastes containing aloe vera, miswak, and propolis were only effective on S. mutans, while toothpastes containing probiotics and enzymes did not show any antimicrobial effect on the bacteria. Among toothpastes with natural ingredients, the theobromine-containing toothpaste showed the highest efficacy on S. mutans, while the aloe vera- and propolis-containing toothpastes had the lowest efficacy (p < 0.05). Conclusions: Theobromine- and chitosan-containing toothpastes, which showed antimicrobial activity against all bacteria, can be recommended as alternatives to traditional toothpastes.

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AKTIVITAS ANTIMIKROBA EKSTRAK DAUN JAMBU BIJI DARI BEBERAPA KULTIVAR TERHADAP Staphylococcus aureus ATCC 25923 DENGAN HOLE-PLATE DIFFUSION METHOD

Journal of Biological Researches ◽

10.23869/bphjbr.9.1.200310 ◽

2003 ◽

Vol 9 (1) ◽

pp. 49-51

Author(s):

Farida Lanawati Darsono ◽

Stephanie Devi Artemisia

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Traditional Medicine ◽

Tween 80 ◽

Antibacterial Activities ◽

Psidium Guajava ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Gram Positive Bacteria ◽

Activity Test

A study has been performed on the antimicrobial activities of jambu biji (Psidium guajava Linn) leaves from several cultivars (red, white and yellow cultivar) against Staphylococcus aureus ATCC 25923 representing the Gram positive bacteria. The reason for conducting this research is that the leaves of jambu biji are frequently used in traditional medicine as a remedy against diarrhoea. The hole-plate diffusion method was used for conducting the antimicrobial activity test with antibiotics (Ampicilline trihidrat) as reference standards. The extracts of jambu biji for each cultivar were obtained by reflux with ethanol 96 percent. The concentrations of the extracts applied to the holes were 10 percent, 20 percent, and 30 percent w/v, the extracts were reconstituted with tween 80 and ethanol 96 percent. Based on the result of the study, it can be concluded that the extract of jambu biji from each cultivar with the concentration of 10 percent, 20 percent, and 30 percent w/v showed antibacterial activities against Staphylococcus aureus ATCC 25923. The result obtained statictically evaluated using Anava Factorial 3x3 and furthery tested for significancy (a = 0.05). Based on the results of study, it can be concluded that the extract of jambu biji leaves from red cultivar, white cultivar and yellow cultivar showed antibacterial activities against Staphylococcus aureus ATCC 25923.

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Antimicrobial activity of Punica grantum leaves

International Journal of Science and Research Archive ◽

10.30574/ijsra.2021.3.2.0162 ◽

2021 ◽

Vol 3 (2) ◽

pp. 214-217

Author(s):

Thammanna Gowda SS ◽

Parimala B Hanumesh ◽

Manjula RV

Keyword(s):

Antimicrobial Activity ◽

Aqueous Ethanol ◽

Total Phenolics ◽

Ethanol Extract ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Human Beings ◽

Disc Diffusion Method ◽

Contagious Diseases ◽

Varied Concentration

Natural phytochemicals isolated from medicinal plants acts as inhibitors for pathogenic microorganisms which causes contagious diseases to human beings. The present study was done to explore the antimicrobial activity of aqueous and aqueous-ethanol extract of Punica grantum leaves. The phytochemical analysis of both the extracts revealed the presence of total phenolics & flavonoids at varied concentration. The antimicrobial activity of the extracts was carried out by disc diffusion method. Both the aqueous and aqueous-ethanol extract showed the antimicrobial activity against S. aureus, Shi. dysentriae & Shi. flexineri. The antimicrobial activity of the Punica grantum leaves extracts could be attributed by the presence of phenolics and flavonoids.

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Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

Scientia Pharmaceutica ◽

10.3390/scipharm87010002 ◽

2019 ◽

Vol 87 (1) ◽

pp. 2 ◽

Cited By ~ 1

Author(s):

Luis Sequeda-Castañeda ◽

Camila Muñoz-Realpe ◽

Crispín Celis-Zambrano ◽

Sandra Gutiérrez-Prieto ◽

Pilar Luengas-Caicedo ◽

...

Keyword(s):

Antimicrobial Activity ◽

Streptococcus Mutans ◽

Porphyromonas Gingivalis ◽

Fusobacterium Nucleatum ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Prevotella Intermedia ◽

Streptococcus Sobrinus ◽

Total Extract ◽

Liquid Fractionation

Berberis goudotii is an endemic Colombian plant found in the paramo ecosystem. It has been used in food preparation and as a medicinal plant for diverse treatments. Additionally, it is used as a mouthwash to strengthen the gums and combat throat irritations and periodontitis. The present research evaluated Berberis goudotii aerial parts extract and fractions antimicrobial activities. Ultrasonic-assisted extraction was used to attain total ethanol-water extract. Solid-liquid fractionation was used to obtain hexane fraction. The residue was dispersed in water and liquid-liquid fractionation was carried-out to acquire dichloromethane, butanol and water fractions. Preliminary phytochemical analysis was performed on total extract and phenol, polyphenol, flavonoid, and proanthocyanidin, while tannin content was quantified. Antimicrobial activity assessment was performed by agar diffusion method using disks and wells employing Ceftazidime as a positive control against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Antimicrobial activity was determined as relative percentage inhibition (RPI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phenols (92.5 ± 7.7 mg GA/10 g), polyphenols (87.7 ± 8.1 mg PG/10 g) and tannins (44.1 ± 4.3 mg PG/10 g) were among the highest secondary metabolites observed. Total extract presented an MBC of 1.0 µg/µL against cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus) and 0.12 µg/µL against bacteria associated with periodontal disease (Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum). Butanol and hexane fractions showed antiperiodontal activity with MBC of 0.12 and 1.0 µg/µL, respectively. In conclusion, Berberis goudotii total extract demonstrated antimicrobial activity against cariogenic and periodontal microorganisms, on the other hand, hexane and butanol fractions displayed antiperiodontal activity.

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Herbal mouthwash formulated with the leaf extract of Jatropha gossypiifolia Linn. (Euphorbiaceae) exhibited in vitro antimicrobial activity against selected oral pathogens

Journal of Pharmacy & Bioresources ◽

10.4314/jpb.v18i3.5 ◽

2021 ◽

Vol 18 (3) ◽

pp. 207-214

Author(s):

Abel Olusola Idowu ◽

Nwamaka Henrietta Igbokwe ◽

Oreoluwa Ayomide Abiodun ◽

Chijioke Ofomata

Keyword(s):

Antimicrobial Activity ◽

Leaf Extract ◽

Antimicrobial Activities ◽

Oral Disease ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Root Bark ◽

Oral Pathogens ◽

Jatropha Gossypiifolia

Increase in the incidence of oral disease, resistance to antibiotics and adverse effect to synthetic medicines has made search for alternative safe, effective and cheaper treatment options imperative, preferably from plant sources. This study evaluated the antimicrobial profile of ethanol extracts of leaf and root bark of Jatropha gossypiifolia and mouthwash formulation containing the leaf extract. Extraction of plant parts was done by cold maceration with 70% ethanol. The antimicrobial activities of the extracts and the formulated mouthwash were evaluated againstStaphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Bacillus subtilis and Candida albicans using Agar well diffusion method. The minimum inhibitory concentration of the extracts on susceptible organisms was determined according to the Clinical Laboratory Standards Institute protocol. The physicochemical parameters of the formulated mouthwash were assessed using standard methods. J. gossypiifolia leaf and root bark extracts exhibited a concentration dependent antimicrobial activity and MIC ranging from 50 to 260 mg/ml against test organisms. The formulated herbal mouthwash showed effective antimicrobial activity and suitable cosmetics properties. This study indicates that the leaf extracts of J. gossypiifolia possess bioactive metabolites with strong antimicrobial activities and its herbal mouthwash formulation has potential in the treatment of various diseases caused by oral pathogens.

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Antimicrobial activity of Guava tree (Psidium Guajava) leaf extract and selected Commercial Antibiotics on Bacterial isolates from Kisubi Hospital

10.51168/sjhrafrica.v1i12.1 ◽

2020 ◽

Author(s):

◽

David Serunjogi

Keyword(s):

Antimicrobial Activity ◽

Psidium Guajava ◽

Diffusion Method ◽

Resistant Bacteria ◽

Drug Resistant ◽

Leaf Extracts ◽

Drug Resistant Bacteria ◽

The World ◽

Guava Leaf ◽

Tree Leaf

Abstract Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.

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