scholarly journals Anti-diabetic activity of Erythrina subumbrans (Hassk.) Merr.

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1826-1831
Author(s):  
Ganesh S ◽  
Vijey Aanandhi M
Keyword(s):  
Inhibitory Concentration ◽  
Standard Sample ◽  
Ethanolic Extract ◽  
Operative Strategy ◽  
Ic50 Value ◽  
Aqueous Ethanolic Extract ◽  
Amylase Inhibitors ◽  
Herbal Plant ◽  
Leaf Part ◽  
Vegetative Leaf

α-amylase inhibitors present in pancreatic region has an operative strategy by controlling the breakdown of starch and helps to minimize the post-prandial hyperglycemia levels. In this study, vegetative (leaf) part of herbal plant Erythrina subumbrans (Hassk.) was assessed for anti-diabetic activity. Aqueous ethanol (80 %) extract was prepared in the different concentration (10, 20, 40, 80, 160, and 320 µg/ml). Acarbose was used as a standard and treated in similar way as that of sample. Control samples were also prepared without standard and sample solutions. A known volume of α-amylase solution was added (0.1mg/mL) was added to standard, sample, control solutions which were preincubated at 37 °C for 15 minutes. Further, known volume of starch solution was added and incubated for 60 min to initiate the reaction. Hydrochloric acid (HCl) and iodine reagent was added to the test tubes and absorbance was measured at 580 nm in UV-Vis spectrophotometer. A strong pancreatic amylase inhibitory activity (>50 %) was obtained from aqueous ethanolic extract with IC50 (half maximal inhibitory concentration) value of 23 µg/ml against standard acarbose with IC50 value of 27 µg/ml. The values endorse Erythrina subumbrans (Hassk.) for further experiments on their potential for managing Diabetes.

scholarly journals Anthelmintic and Antioxidant activity of Aqueous Ethanolic Extract of Erythrina subumbrans (Hassk.) Merr.

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2549-2555
Author(s):  
Ganesh S ◽  
Vijey Aanandhi M
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Crude Extract ◽  
Anthelmintic Activity ◽  
Ethanolic Extract ◽  
Death Time ◽  
Ic50 Value ◽  
Aqueous Ethanolic Extract ◽  
Standard Solution ◽  
Leaf Part

Aqueous Ethanolic extract of vegetative (leaf) part of herbal plant Erythrina subumbrans (Hassk.) was assessed for Anthelmintic and Antioxidant activity. The obtained crude extract was prepared in different concentration, i.e., 50 and 100 mg/mL, against the standard Piperazine citrate, i.e., 10mg/mL for anthelmintic activity. Pheretima posthuma test worms were used during the study and the anthelmintic activity was evaluated based on Paralysis and Death time. Three groups of the test specimen and freshly prepared solutions were used during the study. The average paralysis and death time of standard is 1.4 & 39.59 min (10mg/mL) compared to sample 5.3 & 65.09 min (50mg/mL); 2.5 & 54.08 min (100mg/mL). From the data, aqueous ethanolic extract possesses promising anthelmintic activity compared to piperazine citrate standard. Similarly, antioxidant activity was evaluated for sample at different concentration ranging from 5, 10, 20, 40, 80, 160, and 320 µg/ml premixed with 2.5mL of 0.0135mM DPPH solution. Ascorbic acid was used as a standard and treated in a similar way as that of a sample. Control samples were also prepared without standard and sample solutions. Prepared solutions were vortexed and kept at benchtop condition for 30 minutes and absorbance was measured at 517 nm in a UV-Vis spectrophotometer. The absorbance pattern of the sample is not comparable with that of the standard solution at the same concentration. Aqueous ethanolic extract with IC50 (half maximal inhibitory concentration) value is >320 µg/mL against standard Ascorbic acid with an IC50 value of 15.5 µg/ml. Which witness the crude extract possess less antioxidant activity. 

scholarly journals In-vitro Cytotoxic activity of Papaverine compound isolated from Aqueous Ethanolic leaf extract of Erythrina subumbrans (Hassk.) Merr.

2021 ◽  
Vol 12 (2) ◽  
pp. 1396-1403
Author(s):  
Ganesh S ◽  
Vijey Aanandhi M
Keyword(s):  
Cytotoxic Activity ◽  
Ethanolic Extract ◽  
Chemical Diversity ◽  
Resonance Spectroscopy ◽  
13C Nuclear Magnetic Resonance ◽  
Ic50 Value ◽  
Microplate Reader ◽  
Aqueous Ethanolic Extract ◽  
Isolated Compound

Natural compounds that occur either in pure form or as extract after standardization from medicinal plants, provide a limitless chance for new discovery leads because of consummate chemical diversity. The rising demand for compounds that occur naturally during screening studies, that has therapeutic action predominantly on the vegetative part of the plant has grown across the world. In this study, Papaverine compound was isolated from aqueous Ethanolic extract (80%) from vegetative (leaf) part of herbal plant Erythrina subumbrans (Hassk.)  Merr. Isolated compound was subjected for characterization studies like Thin layer chromatography (TLC), Mass spectrophotometry (MS), Fourier Transform Infra-Red (FTIR), 1H and 13C Nuclear magnetic resonance spectroscopy. Confirmatory study using the aqueous ethanolic crude extract by MS technique and mass library match confirms the presence of Papaverine compound. Further, in-vitro cytotoxic activity using Human Melanoma cell line (A375) was carried out using the isolated compound (papaverine), Aqueous ethanolic extract and standard Cisplatin. Six different Concentration ranging from 3.125 to 100 µg/ml were prepared. Control samples were prepared without standard and samples solution. Absorbance measured at 570 nm in UV-Vis spectrophotometer using a microplate reader and the % of viability was calculated. IC50 value of Aqueous ethanolic extract of Erythrina subumbrans, isolated compound-Papaverine and standard Cisplatin exhibits value of 82.14µg, 23.43 µg & 4.02 µg respectively. 

scholarly journals Cytotoxicity of Mangrove Leaves (Rhizophora) Ethanolic Extract on Cancer Cells

2021 ◽  
Vol 4 (1) ◽  
pp. 43-52
Author(s):  
Haryoto Saroyo ◽  
Nur Fajariyah Maulidah Saputri
Keyword(s):  
Cancer Cells ◽  
Ethanolic Extract ◽  
The Body ◽  
Original Research ◽  
Cytotoxic Test ◽  
Plant Parts ◽  
Ic50 Value ◽  
Abnormal Cells ◽  
Cytotoxicity Effects ◽  
Herbal Plant

Cancer is a disease that can attack any part of the body when the abnormal cells begin to grow  uncontrollably beyond the limit, then attack the connected parts of the body and  spread to other organs. The mangrove (Rhizophora) is a herbal plant that can be used as a treatment for various diseases, one of which is cancer. The mangrove (Rhizophora) plant contains phytochemicals  ranging from fruit, seeds, leaves and roots. This review article aims to examine the cytotoxicity effects of mangrove (Rhizophora)  plants on cancer cells. The library sources in this article review use the Google Scholar and PubMed databases. The inclusion criteria used were articles containing the cytotoxic test of mangrove (Rhizophora) with the last 10 years of publication (2011-2020), original research, there were results of anticancer activity in the form of IC50 and research using   mangrove (Rhizophora).  plant extracts. The exclusion criteria used were articles that did not contain a full text, used plants with different genus, did not have an IC50 value and were not original research. From the journals that have been analyzed, it can be said that the extract of the mangrove (Rhizophora) plant can be developed for cancer treatment. The mangrove (Rhizophora) plant has cytotoxic activity because it contains active compounds in the form of phenolic, flavonoids and terpenoids. Based on the results of the cytotoxic test of mangrove (Rhizophora) extract, the plant parts that have the strongest cytotoxic effect are fruit.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals Phytochemical Analysis, Pharmacological and Safety Evaluations of Halophytic Plant, Salsola cyclophylla

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2384
Author(s):  
Hamdoon A. Mohammed ◽  
Mohsen S. Al-Omar ◽  
Salman A. A. Mohammed ◽  
Ahmad H. Alhowail ◽  
Hussein M. Eldeeb ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
High Concentration ◽  
Halophytic Plant ◽  
Anti Oxidant ◽  
Aqueous Ethanolic Extract ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
Dpph Scavenging ◽  
Anti Inflammation

Salsola cyclophylla, an edible halophyte, is traditionally used for inflammation and pain. To confirm the claimed anti-inflammatory and analgesic properties, a detailed study on respective pharmacological actions was undertaken. The activities are contemplated to arise from its phytoconstituents. The LC-MS analysis of S. cyclophylla 95% aqueous-ethanolic extract revealed the presence of 52 compounds belonging to phenols, flavonoids, coumarins, and aliphatics class. A high concentration of Mn, Fe, and Zn was detected by atomic absorption spectroscopic analysis. The ethyl acetate extract showed the highest flavonoid contents (5.94 ± 0.04 mg/g, Quercetin Equivalents) and Fe2+-chelation (52%) potential with DPPH radicals-quenching IC50 at 1.35 ± 0.16 mg/mL, while the aqueous ethanolic extract exhibited maximum phenolics contents (136.08 ± 0.12 mg/g, gallic acid equivalents) with DPPH scavenging potential at IC50 0.615 ± 0.06 mg/mL. Aqueous ethanolic extract and standard quercetin DPPH radicals scavenging’s were equal potent at 10 mg/mL concentrations. The aqueous ethanolic extract showed highest analgesic effect with pain reduction rates 89.86% (p = 0.03), 87.50% (p < 0.01), and 99.66% (p = 0.0004) after 60, 90, and 120 min, respectively. Additionally, aqueous ethanolic extract exhibited the highest anti-inflammation capacity at 41.07% (p < 0.0001), 34.51% (p < 0.0001), and 24.82% (p < 0.0001) after 2, 3, and 6 h of extract’s administration, respectively. The phytochemical constituents, significant anti-oxidant potential, remarkable analgesic, and anti-inflammatory bioactivities of extracts supported the traditionally claimed anti-inflammatory and analgesic plant activities.

scholarly journals Antimicrobial Activity of Leaves and Flowers of Cassia auriculata linn

1970 ◽  
Vol 46 (4) ◽  
pp. 513-518 ◽  
Author(s):  
V Subhadradevi ◽  
K Asokkumar ◽  
M Umamaheswari ◽  
AT Sivashanmugam ◽  
JR Ushanandhini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Dominant Role ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Cassia Auriculata ◽  
Potential Source ◽  
Wide Range ◽  
Ancient Times

Since ancient times plant as sources of medicinal compounds have continued to play a dominant role in the maintenance of human health. To treat chronic and infectious diseases plants used in traditional medicine contain a wide range of ingredients. In this regard, Cassia auriculata L. (Caesalpiniaceae) is widely used in Ayurvedic medicine as a tonic, astringent and as a remedy for diabetes, conjunctivitis, ulcers, leprosy, skin and liver diseases. The aim of present study was to evaluate the antimicrobial activity of ethanolic extract of Cassia auriculata leaves and flowers (CALE & CAFE). CALE and CAFE exhibited broad spectrum antimicrobial activity against standard strains of Staphylococcus aureus, Escherichia coli and Bacillus subtilis and exhibited no antifungal activity against standard strains of Candida albicans and Aspergillus niger. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) was carried out for CALE and CAFE. The results obtained in the present study indicate that the CALE and CAFE can be a potential source of natural antimicrobial agents. Key words: Cassia auriculata; Antimicrobial activity; Agar well diffusion method. DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9600 BJSIR 2011; 46(4): 513-518

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants

2011 ◽  
Vol 83 (3) ◽  
pp. 1045-1058 ◽  
Author(s):  
Paulo Michel P. Ferreira ◽  
Davi F. Farias ◽  
Martônio P. Viana ◽  
Terezinha M. Souza ◽  
Ilka M. Vasconcelos ◽  
...  
Keyword(s):  
Human Cancer ◽  
Ethanolic Extract ◽  
Cell Number ◽  
Northeastern Brazil ◽  
Human Cancer Cells ◽  
Ic50 Value ◽  
Underlying Mechanisms ◽  
Apoptotic Pathways ◽  
Seed Extracts

This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

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