Green Synthesis of Silver Nanoparticles for Antibacterial Application

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Lena Fadhil AL-Jibouri ◽  
Abdulhussien Mahdi Aljebory
Keyword(s):  
Antibacterial Activity ◽  
Tomato Fruit ◽  
Agar Plate ◽  
Spherical Shape ◽  
Luria Broth ◽  
Nano Particles ◽  
Zone Of Inhibition ◽  
High Concentration ◽  
E Coli ◽  
Bacterial Growth Curve

Tomato, as a fresh or processed product,has a high nutritional value due to its content of bioactive components such as phenolic compounds. Tomato fruit juice is used as a reducing and capping agents for the biosynthesis of silver nano particles (AgNPs). Ultraviolet spectroscopic analysis offered peaks in the range of 396‒420 nm that indicate the production of AgNPs. Silver in ionic or nanoparticle forms has a high antimicrobial activity and is therefore widely used for various sterilization purposes including materials of medical devices and water sanitization. The Scanning electron microscopy images showed that the particles were of mostly spherical shape and confirms the size of NPs that varies from 10 to 30 nm. the bacteriological tests of AgNPs also showed good antibacterial activity against(Escherichia coli)The efficacious bioactivities indicate that the tomato AgNPs can be used efficiently in pharmaceutical and medical industries. The aqueous extract of Lycopersicon esculentum (red tomato) was used for the rapid synthesis of AgNP,which is very simple and eco-friendly in nature. The UV-visible spectroscopy technique was employed to establish the formation of AgNP. For the bacteriological tests,the microorganism E. coli,were inoculated on Luria broth (LB) agar plate in the presence of varied amounts of AgNP. The antibacterial activity was obvious from the zone of inhibition. At concentration 50 μg/ml and above,the AgNP showed a clear zone of inhibition and the minimum inhibitory concentration of AgNP was 20 μg/ml. From the slope of the bacterial growth curve,it has been concluded that the nanoparticles are bacteriostatic at low concentration and bactericidal at high concentration. Therefore,these nanoparticles are believed to act as preventive for bacterial contamination.

Electrospun PU/MgO/Ag Nanofibers for Antibacterial Activity and Flame Retardency

2021 ◽  
pp. 0887302X2110094
Author(s):  
V. Mamtha ◽  
H. N. Narasimha Murthy ◽  
V. Pujith Raj ◽  
Prashantha Tejas ◽  
C. S. Puneet ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Melting Point ◽  
Protective Clothing ◽  
Electrospun Nanofibers ◽  
Synthesis And Characterization ◽  
Zone Of Inhibition ◽  
Solution Combustion ◽  
E Coli ◽  
Afterglow Time

Antibacterial activity and fire retardation are equally desired for protective clothing. For achieving this, AgNP and MgO are independently researched as nanofillers in Polyurethane based electrospun nanofibers and their synergistic effect is scarcely addressed. This article reports synthesis and characterization of MgO of 70.01 nm and AgNP of 51 to 76 nm by solution combustion and hydrothermal routes respectively and their incorporation in electrospinning of Polyurethane. Flow rate 1 ml/hr, applied voltage 13 kV, tip to collector distance 15 cm were adopted for the electrospinning. Nanofibers of 65 nm were obtained for PU/MgO (3 wt. %) and 106 nm for PU/MgO (3 wt. %)/Ag (1 wt. %). Addition of MgO increased the melting point, after flame time and afterglow time. Incorporation of AgNP improved antibacterial activity. PU/MgO/Ag (2 wt. %) exhibited zone of inhibition of 2.1 cm and 3 cm against E. Coli and S. Aureus, respectively.

Antibacterial Function of Chromium Nanoparticles Against K. Pneumonia, E. coli and P. typhus

2020 ◽  
Author(s):  
Giriraj Tailor ◽  
Jyoti Chaudhary ◽  
Ajit Joshi ◽  
Deepshikha Verma ◽  
Osahon Michael
Keyword(s):  
Antibacterial Activity ◽  
Decomposition Method ◽  
Clinical Importance ◽  
Gram Negative Bacteria ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Plate Method ◽  
E Coli ◽  
Thermal Decomposition Method ◽  
Chromium Nanoparticles

Abstract The bioactive chromium nanoparticles were synthesized by calcination followed by thermal decomposition method. The antibacterial activity of chromium nanoparticles diffused in Dimethyl sulphoxide (DMSO). The antibacterial activity of chromium nanoparticles carried out against significant human pathogens (gram negative bacteria) viz, K. pneumonia, E. coli and P. typhus using agar diffusion cup plate method at 100 µg/ml concentration. The highest zone of inhibition was observed (12.0 mm) against K. pneumonia and lowest zone of inhibition (7.0 mm) E. coli. Thus, the outcomes of these studies suggest that synthesized chromium nanoparticles are of clinical importance.

scholarly journals Investigating In Vitro Antibacterial Activities of Medicinal Plants Having Folkloric Repute in Ethiopian Traditional Medicine

2019 ◽  
Vol 24 ◽  
pp. 2515690X1988627 ◽  
Author(s):  
Mekonnen Sisay ◽  
Negussie Bussa ◽  
Tigist Gashaw ◽  
Getnet Mengistu
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Antimicrobial Agents ◽  
Bacterial Isolates ◽  
Zone Of Inhibition ◽  
Traditional Use ◽  
Gram Negative ◽  
E Coli ◽  
Slow Development

Medicinal plants are targeted in the search for new antimicrobial agents. Nowadays, there is an alarmingly increasing antimicrobial resistance to available agents with a very slow development of new antimicrobials. It is, therefore, necessary to extensively search for new agents based on the traditional use of herbal medicines as potential source. The antibacterial activity of 80% methanol extracts of the leaves of Verbena officinalis (Vo-80ME), Myrtus communis (Mc-80ME), and Melilotus elegans (Me-80ME) was tested against 6 bacterial isolates using agar well diffusion technique. In each extract, 3 concentrations of 10, 20, and 40 mg/well were tested for each bacterium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Vo-80ME and Mc-80ME exhibited promising antibacterial activity against Staphylococcus aureus with the highest zone of inhibition being 18.67 and 26.16 mm, respectively at concentration of 40 mg/well. Regarding gram-negative bacteria, Vo-80ME exhibited an appreciable activity against Escherichia coli and Salmonella typhi. Mc-80ME displayed remarkable activity against all isolates including Pseudomonas aeruginosa with the maximum zone of inhibition being 22.83 mm. Me-80ME exhibited better antibacterial activity against E coli, but its secondary metabolites had little or no activity against other gram-negative isolates. The MIC values of Vo-80ME ranged from 0.16 to 4.00 mg/mL. The lowest MIC was observed in Mc-80ME, with the value being 0.032 mg/mL. Mc-80ME had bactericidal activity against all tested bacterial isolates. Mc-80ME showed remarkable zone of inhibitions in all tested bacterial isolates. Besides, Vo-80ME showed good antibacterial activity against S aureus, E coli, and S typhi. Conversely, Me-80ME has shown good activity against E coli only. Generally, M communis L and V officinalis have good MIC and MBC results.

scholarly journals Study of Antibacterial Activity of Chenopodium album Leaves Extract

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Kaur M. ◽  
Sharma S. ◽  
Garg S. ◽  
Arora M.
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Chenopodium Album ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
Zone Of Inhibition ◽  
Plate Method ◽  
E Coli ◽  
Test Organisms

This study describes the antibacterial activities of three different solvent extracts of leaves of Chenopodium album. Methanol, acetone and chloroform extracts of C. album were prepared. The antibacterial activity was assessed using well plate method and were examined for the size of zone of inhibition. Different extracts were investigated against the test organisms namely Lactobacillus, Bacillus subtilis and Escherichia coli. The maximum activity was observed at 100% concentration of different extracts of leaves. The maximum zone of inhibition for 100% concentration were observed as E. coli (19 mm) and Lactobacillus (19 mm) in diameter respectively. C. album did not show any antibacterial activity against B. subtilis. Antibacterial activity was compared with standard Amoxicillin and it was found to be 23 mm diameter for Lactobacillus and 25 mm for both E. coli and B. subtilis in terms of zone of inhibition.

Synthesis of Silver Nanoparticle Using Bioactive Phenolic Compound Extracts of Leucas aspera and Leucas cephalotes and Evaluation of its Antibacterial Activity

2021 ◽  
Vol 6 (4) ◽  
pp. 310-314
Author(s):  
M. Salma Kausar ◽  
B.K. Manjunatha ◽  
C. Purvika ◽  
Mizba Farkana
Keyword(s):  
Antibacterial Activity ◽  
Silver Nanoparticle ◽  
Crude Extract ◽  
Bioactive Compound ◽  
Xrd Analysis ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Leucas Aspera ◽  
Phenolic Extract

The present study was carried out to investigate the antibacterial activity of the bioactive phenolic extract from Leucas aspera and Leucas cephalotes. The phenolic compounds were extracted using water: ethanol (1:3, v/v) by hydroethanolic extraction method. The hydroethanolic extracts were subjected to qualitative and FTIR analysis as a confirmatory step for the presence of phenolics. Synthesis of silver nanoparticle from both plants was carried out by acid hydrolysis method and subjected to UV-visible spectrophotometry, SEM, TEM and XRD analysis, for confirmation of tagged bioactive compound to AgNO3. The nanoparticle size distribution ranged between 50-94 nm in L. aspera and 40-67 nm in L. cephalotes. The antibacterial study was carried out using both crude phenolic extract and synthesized nanoparticles and tested against 5 pathogens namely Escherichia coli (ATCC® 8739™), Pseudomonas aeruginosa (ATCC® 25619™), Staphylococcus aureus (ATCC® 6538™), Bacillus subtilis (ATCC® 11774™) and Klebsiella pneumonia (ATCC® 13882™) for their antibacterial activity. From present study, the crude extract of L. cephalotes showed good antibacterial effect against test pathogen species wherein highest inhibition was observed in, P. aeruginosa, followed by B. subtilis and S. aureus with an average zone of inhibition of 23, 14 and 12 mm, E. coli and K. pneumonia measured 9 and 7 mm. The crude extract of L. aspera showed the highest inhibition in P. aeruginosa followed by S. aureus and E. coli with an average zone of inhibition of 12,11 and 10 mm B. subtilis and K. pneumonia measured 8 and 7 mm. Statistical analysis was calculated using One way ANOVA and was found to be statistically significant with p < 0.05.

scholarly journals Phytochemical And Antibacterial Activityof Leave Extracts Of Guiera Senegalensis Lam On Selected Species Of Gram Positive And Gram Negative Bacteria

2013 ◽  
Vol 2 (1) ◽  
pp. 262-268 ◽  
Author(s):  
IA Mamman ◽  
MA Isa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Clinical Isolates ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Klebsiella Species ◽  
E Coli ◽  
Guiera Senegalensis

The aim of this study was to determine the preliminary phytochemical component and antibacterial activity of the leaves extract of Guiera senegalensis lam (Combretaceae) against three clinical isolates (Staphylococcus aureus, E. coli and Klebsiella species) using standard method of analysis. The test for phytochemical component revealed the presence of alkaloid, anthraquinolones, tannin and phlobatanins. The result of antibacterial activity showed that the ethanolic extract exhibit higher zone of inhibition against all the clinical isolates, with E. coli and Klebsiella species showed zone of inhibition of 35mm followed Staphylococcus aureus 30mm. Similarly, both aqueous and methanolic extract were sensitive to all the clinical isolate except Klebsiella species which showed resistance to the methanolic extract of the leaves. The results obtained in this research imply that the leaves extract of Guiera senegalensis lam could be useful in the treatment of infections caused by Staphylococcus aureus, E. coli and Klebsiella species. International Journal of Environment, Volume-2, Issue-1, Sep-Nov 2013, Pages 262-268 DOI: http://dx.doi.org/10.3126/ije.v2i1.9226

scholarly journals Comparative Study of Antibacterial Activity of Juice and Peel Extract of Citrus Fruits

2019 ◽  
Vol 6 ◽  
pp. 82-88 ◽  
Author(s):  
Alisha Shakya ◽  
Bhawana Luitel ◽  
Pragati Kumari ◽  
Ritu Devkota ◽  
Puspa Raj Dahal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Diffusion Method ◽  
Citrus Limon ◽  
Citrus Fruits ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Gram Positive ◽  
E Coli ◽  
Bacillus Spp ◽  
Peel Extract

Objectives: This study was aimed to compare the antibacterial activity of peel and juice extracts of citrus fruits. Methods: The antibacterial activity of juice and peel extract of five selected citrus fruits [C1- Citrus reticulata (mandarin orange), C2- Citrus limetta (sweet lime), C3- Citrus aurantifolia (lime), C4- Citrus limon (lemon) and C5- Citrus maxima (pommelo)] on three Gram positive and five Gram negative bacterial strains were examined. The juice and peel extract of citrus fruits was obtained by using absolute ethanol. The obtained extracts were dissolved in 1ml 10% v/v DMSO which was taken to carry out the antibacterial susceptibility assay using agar well diffusion method. Results: The peel extracts of C1 and C2 were found to be more effective against the given Gram-positive bacteria than the juice extracts with maximum zone of inhibition (20.33±1.527) and (15.33±0.577) against Bacillus spp respectively. While the juice extracts of C3 and C4 were more effective against both Gram positive and negative bacteria than their peel extracts with maximum zone of inhibition (26.66±1.15) against S. aureus ATCC 25923 and (20±1) against E. coli ATCC 25922 respectively. The juice extract of C5 was found to be more effective against both Gram positive and negative bacteria than its peel extract with maximum zone of inhibition (18.66±1.154) against E. coli ATCC 25922 except for Bacillus spp in which peel extract showed maximum zone of inhibition i.e. (15±1) Conclusion: This study suggests that these juice and peel extracts may have beneficial antibacterial roles that can be exploited in controlling unwanted bacterial growth.

scholarly journals Phytochemical and Antimicrobial Studies of the Crude Extracts of the Leaves of Carica papaya Linn (Pawpaw) and Psidium guajava Linn (Guava)

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Priscilla Alexander ◽  
Ismaila Yada Sudi ◽  
Martin Tizhe
Keyword(s):  
Antibacterial Activity ◽  
Carica Papaya ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Psidium Guajava ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Active Inhibition ◽  
Antimicrobial Studies ◽  
Alternative Antibiotic

Psidium Guajava (Guava) and Carica Papaya leaves which have some ethnomedicinal applications were investigated. Phytochemical screening of their leaves revealed the presence of flavonoids, saponins, terpenoids, steroids, tannins and glycosides. Antimicrobial screening of the crude ethanolic extracts showed activity against Staphylococcus aureus, Streptococcus faecalis, and Escherichia coli. The minimum inhibitory concentration (MIC) for P. gujava on the organism was found to be 5.00 mg/ml against S. aureus, E. coli and S. faecalis, while that of C. papaya leaves is 10.00 mg/ml against S. aureus, E. coli and 8.00 mg/ml against S. faecalis respectively. C. papaya ethanolic extract showed more active inhibition against S. aureus with mean zone inhibition of 9.54 ± 0.03.  P.   gujava ethanolic extract has more active inhibition against E. coli with antibacterial activity with mean zone of inhibition of 10.44±0.02 and S. faecalis with mean zone of inhibition of 6.72 ± 0.01 respectively.  This study showed that the leaves extract of these plants are good sources of bioactive compounds. Demonstration of antibacterial activity against the test isolates is an indication that there is possibility of sourcing alternative antibiotic substances in these plants for the production of newer antibacterial agents. These plants therefore, could be an important source of medicine for the treatment of various diseases.

Phytochemical characterization and antibacterial activity of Eclipta alba (L.) L. (Asteraceae) against some dental micro flora from Mewar region of Rajasthan, India

2018 ◽  
Vol 7 (3) ◽  
pp. 140-146
Author(s):  
N. R. Keer ◽  
J. Singh ◽  
K. L. Meena
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Micrococcus Luteus ◽  
Bioactive Components ◽  
Zone Of Inhibition ◽  
Standard Methods ◽  
E Coli ◽  
Eclipta Alba ◽  
Phytochemical Investigation

In the current study, phytochemical characterization and antibacterial activi-ty of Eclipta alba (L.) L. leaf extract against some dental micro flora have been investigated. Plant species was collected from Chittorgarh district Mewar region of Rajasthan. Dried leaves were subjected to methanol extrac-tion. Phytochemical investigation was carried out to determine the possible bioactive components in the methanol extract of E. alba leaves using standard methods. All concentrations of the extract exhibited considerable antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Micrococcus luteus. During the study 100 mg/ml extract showed maximum zone of inhibition 13 mm against E. coli, 12 mm against P. aeruginosa and 14 mm zone of inhibition against B. subtilis, 12 mm zone of inhibition against M. luteus.

scholarly journals Antibacterial Activity of Two Flavans from the Stem Bark of Embelia schimperi

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Babe Guyasa ◽  
Yadessa Melaku ◽  
Milkyas Endale
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Fungal Infections ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Techniques ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
E Coli

Embelia schemperi Vatke is one of the medicinal plants used traditionally for treatment of intestinal tape worm, dysmenorrheal, bacterial, and fungal infections. Phytochemical screening test of the dichloromethane/methanol (1 : 1) and methanol extracts revealed the presence of phenols, alkaloids, tannins, and flavonoids whereas terpenoids, glycoside, and phytosterols were absent. Silica gel column chromatographic separation of the methanol extract afforded 3,5,7,3′,4′-pentahydroxyflavan, named epicatechin (1), along with a close flavan derivative (2). Structures of the compounds were elucidated by spectroscopic techniques (1D and 2D NMR, FTIR, and UV-Vis). The crude extracts and isolated compounds were screened for in vitro antibacterial activity against strains of Klebsiella pneumoniae, Escherichia coli, Cryptococcus neoformans, Shigella dysentriae, and Staphylococcus aureus. Epicatechin (1) exhibited comparable antibacterial activity against S. aureus and E. coli (15 and 12 mm zone of inhibition, resp.) to that of the control antibiotic gentamicin, with zone of inhibition of 15 and 12 mm, respectively, at a concentration of 20 µg/mL.

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