scholarly journals The results of immunohistochemical study of signaling pathways’ markers of proliferation in uterine leiomyoma in women using hormonal contraception.

Morphologia ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 67-72
Author(s):  
E.P. Finkova
Keyword(s):  
Uterine Leiomyoma ◽  
Ethinyl Estradiol ◽  
Hormonal Contraception ◽  
Group Iv ◽  
Group Viii ◽  
Group Vi ◽  
Ki 67 ◽  
Group V ◽  
Group Iii ◽  
Group I

Objective. To elucidate the influence of various components of hormonal contraception in women with uterine leiomyoma (UL) on the key molecular and cellular mechanisms of its proliferation. Methods. Antigen Ki-67, estrogen receptors (ER) and progesterone receptors (PR) were determined by immunohistochemical methods in 230 samples of UL preparations obtained during myomectomy. Depending on the composition of the components of hormonal contraceptives that women used for 12 months before the operation, 8 study groups were created: Group I - control, without the use of any hormonal contraception; Group II - the use of COCs containing 20 μg of ethinylestradiol and 0.075 mg of gestaden; Group III - COCs (30 μg ethinylestradiol and 0.075 mg gestaden) Group IV - COCs (30 μg ethinylestradiol and 0.15 mg desogestrel) Group V - COCs (30 μg ethinyl estradiol and 0.15 mg levonorgestrel) Group VI - COCs (30 μg ethinyl estradiol and 2 mg dienogest) Group VII - COCs (30 μg ethinylestradiol and 3 mg drospirenone) Group VIII - (intrauterine levonorgestrel releasing system (IUD-LNG). Results. In UL samples from group I, an increase of Ki-67 positive cells in 3.4 times was observed (3.1 ± 0.03%; p <0.04) in comparison with intact myometrium (IM) (0.9 ± 0.06%), which is evidence of a higher cell proliferation in the UL, a 3.1-fold increase in the H-index of ER expression - 39.4 ± 4.3 (p <0.05) versus 12.9 ± 1.6 in the group with IM I and in 2.6 times of PR expression - 21.1 ± 1.7 (p <0.05) compared to IM - 8.2 ± 1.4, which may indicate a greater sensitivity of UL to sex hormones and their promoter role in UL proliferation. Expression of Ki-67 in UL samples in women taking COCs, which included dienogest (1.8 ± 0.03%, p <0.05) - group VI and desogestrel (1.9 ± 0.03%, p <0.05) - group IV, was, 42.0% and 38.8% respectively, what ois less than in group I UL, which can be regarded as the cytoprotective effect of the progestogen component of COC on the mitotic activity of UL cells. A positive trend in the expression of Ki-67 persisted when women used COCs containing gestodene (2.1 ± 0.02%; p <0.05) - group III and levonorgestrel (2.2 ± 0.04%, p <0.05) - group V, in which the expression of Ki-67 was shown by a smaller number of PM cells, respectively, by 32.3% and 25.8% than in group I PM, and also to a lesser extent - in group VIII (COC with droperidone), where the mean value of Ki-67 expression in LM samples was 2.6 ± 0.02% and was 16.9% less than in LM group I. An increase in the dose of ethinyl estradiol in COCs from 20 μg (group II) to 30 μg (group III) did not significantly affect the expression of Ki-67, therefore, the content of estrogens in modern low-dose COCs does not contribute to an increase in proliferation in the LM, and the non-contraceptive antiproliferative effect is associated exclusively with biological and the pharmacological properties of individual gestagens in the composition of COCs. It was proved that the studied COCs did not significantly affect the expression of ER and PGR. There was no significant difference in the expression of the Ki-67 marker (2.9 ± 0.04%, p <0.05) in UL cells in women using LNG-IUD for contraception, compared with group I. Conclusion. The results of the study have shown that when choosing a drug for hormonal contraception in women with UL, preference should be given to combined hormonal drugs that contain progestogens with the most pronounced antiproliferative properties (dienogest, desogestrel and levonorgestrel).

scholarly journals Effect of a Diet Composed of Genetically Modified Feed Components on the Selected Immune Parameters in Pigs, Cattle, and Poultry

2013 ◽  
Vol 57 (2) ◽  
pp. 209-217 ◽  
Author(s):  
Dariusz Bednarek ◽  
Katarzyna Dudek ◽  
Krzysztof Kwiatek ◽  
Małgorzata Świątkiewicz ◽  
Sylwester Świątkiewicz ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Genetically Modified ◽  
Group Iv ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Immune Parameters ◽  
Group I ◽  
Toxin Protein ◽  
Gm Soybean

Abstract The aim of the study was to evaluate the immune effects of genetically modified (GM), insect resistant corn (MON810) expressing toxin protein of Bacillus thuringiensis, and glyphosate-tolerant soybean meal (Roundup Ready MON-40-30-2), which are used as the feed mixture components in domestic animals. The study was conducted on 60 pigs (36 fatteners and 24 sows), 20 calves, 40 broilers, and 40 laying hens. Each species was divided into four basic nutritional groups: group I (control) - conventional feed, group II - feed consisted of GM soybean meal and non-modified corn, group III - non-modified soybean meal and GM corn, group IV - GM soybean meal and GM corn. Moreover, in the experiment on fatteners two additional groups were formed: group V - animals fed both conventional soybean meal and bruised grain, and group VI - GM soybean meal and conventional bruised grain. The results of study did not reveal any significant effect of feed mixtures containing GM components on the immune response in all animals regardless of their species and technological producing groups.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

2012 ◽  
Vol 27 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Conceição Aparecida Dornelas ◽  
Francisco Vagnaldo Fechine-Jamacaru ◽  
Irineu Lima Albuquerque ◽  
Hemerson Iury Ferreira Magalhães ◽  
Adjair Jairo Silva de Souza ◽  
...  
Keyword(s):  
Group Iv ◽  
The Other ◽  
Control Group ◽  
Group Viii ◽  
Group Vi ◽  
Group V ◽  
Induction Group ◽  
Rat Bladder ◽  
Bladder Carcinogenesis ◽  
Group I

PURPOSE: To determine the effects of green propolis extracted in L-lysine (WSDP) and of L- lysine for 40 weeks on induced rat bladder carcinogenesis. METHODS: The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous; and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water. RESULTS: The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions. CONCLUSION: The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

ВЗАИМОСВЯЗЬ ПРОДУКТИВНЫХ ПОКАЗАТЕЛЕЙ КОРОВ ЧЕРНО-ПЕСТРОЙ ПОРОДЫ С ВОСПРОИЗВОДИТЕЛЬНЫМИ КАЧЕСТВАМИ

2019 ◽  
pp. 39-42
Author(s):  
G.Yu. BEREZKINA ◽  
S.L. VOROBYEVA ◽  
E.M. KISLYAKOVA ◽  
A.A. KOREPANOVA
Keyword(s):  
Experimental Studies ◽  
Live Weight ◽  
Group Iv ◽  
Reproductive Capacity ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
The Republic ◽  
Breeding Farms

Изучено влияние возраста и живой массы при первом осеменении на воспроизводительные качества коровпервотелок чернопестрой породы в племенных заводах Удмуртской Республики. Исследования проводились в период с 2015 по 2018 гг. Для проведения исследований были сформированы 6 групп животных в зависимости от возраста первого осеменения. В I группу вошли коровы с возрастом первого осеменения до 13 мес, во II от 13,1 до 15 мес, в III от 15,1 до 17, в IV от 17,1 до 19, в V от 19,1 до 21 и в VI группу от 21 мес и старше. При этом живая масса телок в I группе в среднем составила 349,5 кг, во II 370,9, в III 387,7, в IV 400,2, в V 420,7 и в VI группе 440,2 кг. Анализ возраста первого осеменения в племенных хозяйствах республики показал, что средний возраст плодотворного осеменения в 2018 году составил 17,0 мес. с живой массой 394 кг. По результатам экспериментальных исследований нами получено, что лучшие воспроизводительные показатели и молочная продуктивность выявлена у коровпервотелок, которых осеменяли в возрасте 14,6 мес. В данной группе продолжительность сервиспериода составила 127,3 дня, коэффициент воспроизводительной способности 0,90, выход телят на 100 коров составил более 85,3. Удой за 305 дней лактации, у коровпервотелок второй опытной группы составил 6332,3 кг с массовой долей жира 3,65 и белка 3,15 .The influence of age and live weight at the first insemination on reproductive qualities of cowsborn black and white breed in breeding farms of the Udmurt Republic was studied. The studies were conducted between 2015 and 2018. For the research 6 groups of animals were formed depending on the age of the first insemination. Group I included cows with the age of first insemination up to 13 months, in group II from 13.1 to 15 months, in III group from 15.1 to 17 months, in group IV from 17.1 to 19 months, in group V from 19.1 to 21 months and in VI group from 21 months and more. At the same time, the live weight of heifers in group I averaged 349.5 kg, in group II370.9 kg, in group III 387.7 kg, in group IV 400.2 kg, in group V 420.7 kg and in group VI 440.2 kg. The analysis of the age of the first insemination in breeding farms of the Republic showed that the average age of fruitful insemination in 2018 was 17.0 months with a live weight of 394 kg. According to the results of experimental studies, we obtained that the best reproductive performance and milk productivity was revealed in cows, which were inseminated at the age of 14.6 months. In this group, the duration of the service period was 127.3 days, the coefficient of reproductive capacity 0.90, yield of calves per 100 cows. Milk yield for 305 days of lactation, in cows of the second experimental group was 6332.3 kg with a mass fraction of fat 3.65 and protein 3.15.

scholarly journals The The Influence of Wharton Jelly Mesenchymal Stem Cell toward Matrix Metalloproteinase-13 and RELA Synoviocyte Gene Expression on Osteoarthritis

2019 ◽  
Vol 7 (5) ◽  
pp. 701-706 ◽  
Author(s):  
Vivi Sofia ◽  
Ellyza Nasrul ◽  
Menkher Manjas ◽  
Gusti Revilla
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Inflammatory Responses ◽  
Group Iv ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

BACKGROUND: Therapy for osteoarthritis (OA) with satisfactory results has not been found to date. In OA pathogenesis, RELA gene involved in cartilage degradation and MMP-13 in degrade cartilage, as a member family of NF-ĸβ genes, RELA serves to modulate inflammatory responses and activates pro-inflammatory cytokines. AIM: This study aims to identify the influence of Wharton Jelly Mesenchymal Stem Cell (MSC-WJ) on MMP-13 and RELA expression gene in synoviocyte by in vitro. MATERIAL AND METHODS: This research is pure experimental research. The sample used derived from synovial tissue of OA patients who underwent Total Knee Replacement (TKR) surgery. This study was divided into six groups treated with 4 replications. Group I and II (control groups) were synoviocyte of OA incubated for 24 and 48 hours, respectively. Group III and IV were MSC-WJ incubated for 24 and 48 hours, respectively. Group V and VI were Synoviocyte-MSC-WJ co-culture group incubated for 24 and 48 hours, respectively. Identification of MMP-13 and RELA gene expression in each group was performed by using qPCR. RESULT: The results showed that MSC-WJ reduced MMP-13 gene expression after co-culture for 24 and 48 hours in OA synoviocyte. The highest gene expression of MMP-13 was in Group I and II (1.00 ng/μl), followed by Group III (0.41 ng/μl), Group IV (0.24 ng/μl), Group V (0.13 ng/μl), and Group VI (0.04 ng/μl). MSC-WJ administration also decreased RELA gene expression. The highest gene expression of RELA gene was in Group I and II (1.00 ng/μl), Group V (0.67 ng/μl), Group III (0.58 ng/μl), Group IV (0.16 ng/μl), and Group VI (0.16 ng/μl). CONCLUSION: This study concluded that MSC-WJ in OA synoviocyte significantly reduced the expression of MMP-13 and RELA gene (p <0.05).

scholarly journals Treatment of Bothrops alternatus envenomation by Curcuma longa and Calendula officinalis extracts and ar-turmerone

2005 ◽  
Vol 57 (1) ◽  
pp. 7-17 ◽  
Author(s):  
M.M. Melo ◽  
G.G. Habermehl ◽  
N.J.F. Oliveira ◽  
E.F. Nascimento ◽  
M.M.B. Santos ◽  
...  
Keyword(s):  
Curcuma Longa ◽  
Group Iv ◽  
Calendula Officinalis ◽  
Group Vi ◽  
Group V ◽  
Efficient Treatment ◽  
Sterile Saline ◽  
Group Iii ◽  
Group I ◽  
Bothrops Alternatus

It was investigated the efficiency of two extracts of plants and one fraction of their properties against the local effects of bothropic envenomation. Bothrops alternatus venom (1.25µg) diluted in 100µl of sterile saline solution was inoculated (intradermally) into the shaved dorsal back skin of 30 New Zealand rabbits. The animals were divided in six groups receiving the following treatments: group I: subcutaneous application of Curcuma longa extract (1.0ml); group II: topic treatment of Curcuma longa hydroalcoholic extract (1.0ml); group III: topic application of ar-turmerone in vaseline (1.0g); group IV: topic application of Curcuma longa methanolic extract (1.0ml); group V: topic application of Calendula officinalis ointment (1.0g); group VI: topic application of saline (1.0ml). These treatments were done at 30 minutes, and at 2, 4, 24 and 72 hours after venom inoculation. Intensity of local edema, hemorrhagic halo and necrosis were evaluated until 168h after that. Additionally, seven days after the Bothrops venom inoculation, blood was collected from heart with and without EDTA (10%) for hemogram and biochemical parameters (total protein, blood urea nitrogen, creatinine, and fibrinogen) and all the animals were anesthetized, sacrificed by ether inhalation and submitted to necropsy. Fragments of tissues were taken for histopathological evaluation. The most efficient treatment for inhibition of edema, necrosis and local hemorrhage after Bothrops alternatus venom was the topic application of ar-turmerone.

scholarly journals Shear Bond Strength of Orthodontic Brackets Cured with Different Light Sources under Thermocycling

2010 ◽  
Vol 04 (03) ◽  
pp. 257-262 ◽  
Author(s):  
Oral Sokucu ◽  
Seyda Herguner Siso ◽  
Firat Ozturk ◽  
Ruhi Nalcaci
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Group Iv ◽  
Light Sources ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Light Curing

Objectives: The aim of this study was to compare the shear bond strength (SBS) of two different light sources under different thermal cycle frequencies.Methods: Ninety human premolar teeth were divided into six groups, in which three groups consisted of halogen groups – Group I was cured with halogen without thermocycle, Group II cured with halogen with 500 thermocycles, and Group III cured with halogen with 10,000 thermocycles. The other three groups consisted of Plasma arc (PAC) group – Group IV was cured with PAC without thermocycle, Group V cured with PAC thermocycled 500 cycles, and Group VI cured with PAC thermocycled 10,000 cycles. Two way ANOVA was used to compare the SBS between the groups, and chi squaretest was used to compare the bond failure of ARI scores among the groups.Results: A significant difference was found for curing lights (P<.001) and thermocycling (P<.01). However, there was no interaction between light curing and thermocycling (P=.177). The halogen groups demonstrated higher mean SBS than the PAC groups. Both groups showed a significant reduction between no cycles and 10,000 cycles (P<.05).Conclusions: Both light sources showed favorable SBS performance without the thermocycle procedure. Thermocycling significantly decreased the SBS values of both groups. (Eur J Dent 2010;4:257- 262)

Assessment of Capability of Lithium Disilicate Glass Ceramics to Reproduce the A2 Shade and to Mask A4 Substrates

2021 ◽  
Vol 9 (1) ◽  
pp. 5-9
Author(s):  
Samarla Aruna Kumari ◽  
Averneni Prema Latha Prema Latha ◽  
Naveen Reddy ◽  
Rohit Sharma ◽  
Mahesh Melkundi ◽  
...  
Keyword(s):  
Glass Ceramics ◽  
Lithium Disilicate ◽  
Group Iv ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Color Differences ◽  
Shade Guide

Aim: Capability of lithium disilicate glass ceramics to reproduce the A2 shade and to mask A4 substrates.Materials and Method: 32 lithium disilicate ceramic specimens were classified materials into 8 groupsof 4 each. Group I- III high translucency (HT) monolayer discs, group IV- VI had low translucency (LT)monolayer discs, group VII- VIII had medium translucency (MO) bilayer discs. A2 shade tab shade guide(VITA classical A1-D4) was used for CIE L*a*b* measurements of control. Color differences using thresholdswas as follows: for imperceptible (?E < 1.0), perceptible (1.0 < ?E < 3.3), and clinically unacceptable (?E> 3.3) differences.Results: ?E in group IV exhibited highest (9.92±0.21) and group III lowest (8.08± 0.25) value. When thecomplex was compared to the A4 substrate, significant difference among all groups (p < 0.05) was found.?E values when the complex was compared to the A2 shade tab, group III exhibited highest (4.82±0.16)and group V lowest (0.72±0.06) value. In groups I specimens had acceptable differences, group II hadperceptible differences, group III had perceptible differences, group IV had acceptable differences, group Vhad imperceptible differences, group VI and VII had acceptable differences and group VIII had imperceptibledifferences.Conclusion: Authors suggested to use LT ceramics with less thickness in the range of 0.5 mm- 0.7 mm tomask discoloration.

scholarly journals Helicobacter Pylori Eradication with Beta Carotene, Ascorbic Acid and Allicin

2001 ◽  
Vol 44 (3) ◽  
pp. 97-100 ◽  
Author(s):  
Cem Koçkar ◽  
Mustafa Öztürk ◽  
Nüket Bavbek
Keyword(s):  
Ascorbic Acid ◽  
Beta Carotene ◽  
Group Iv ◽  
Group Vi ◽  
Group V ◽  
Helicobacter Pylori Eradication ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I

In this study, in vivo effectiveness of ascorbic acid (AA), beta carotene (BC) and allicin in HP eradication were evaluated. 210 patients who are HP positive in biopsy were involved in this study. The patients randomised to seven treatment groups (each group consisting of 30 patients). The first group was given standard eradication treatment (lansaprasol 30 mg bid, clarithromycin 500 mg bid, amoxicillin 1 g bid for 14 days). Second group received AA 1000 mg/day in addition to the standard treatment. Third group received only AA 1000 mg/day for 14 days. Fourth group was treated with standard regiment plus 120 mg/day BC. Fifth group was given only BC 120 mg/day for 14 days. Sixth group was given standard regiment and allicin 4200 μg/day. Seventh group received only Allicin 1200 μg/day for 14 days. The eradication was achieved in 20 (66.6 %) in group I, 15 (50 %) in group II, 3 (10 %) in group III, 15 (50 %) in group IV, 0 (0 %) in group V, 27 (90 %) in group VI and 7 (23.3 %) in group VII. Allicin seemed to be potentially effective agent for HP eradication but ascorbic acid, beta caroten was found to be ineffective.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

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