scholarly journals State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol

2021 ◽  
Vol 26 (2) ◽  
pp. 26-32
Author(s):  
A.Ye. Demkovych ◽  
Yu.I. Bondarenko ◽  
O.O. Fastovets ◽  
A.O. Hrad ◽  
P.A. Hasiuk ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Soft Tissues ◽  
Pathological Process ◽  
Calibration Graph ◽  
The State ◽  
Experimental Animals ◽  
Periodontal Tissues ◽  
Tissue Homogenates ◽  
Experimental Periodontitis

The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7th, 14th and 30th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7th to the 14th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30th day, increase in bone resorption continued  as compared to the 7th and 14th day. During the correction of disorders resulted from  the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.

scholarly journals REACTIVE OXYGEN AND NITROGEN SPECIES ROLE IN EXPERIMENTAL PERIODONTITIS DEVELOPMENT

2018 ◽  
Author(s):  
A. Ye. Demkovych
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Blood Serum ◽  
Inflammatory Process ◽  
Reactive Oxygen ◽  
Nitrogen Species ◽  
Initial Development ◽  
Periodontal Tissues ◽  
Experimental Periodontitis ◽  
Nitric Oxide Metabolites

Introduction. Activation of lipid peroxidation is one of the trigger mechanisms of periodontium injury, which is primary caused by cellular damage. Reactive oxygen and nitrogen species (RONS) are able to cause damage to a cell as well as final products of lipid peroxidation, including unsaturated aldehydes and other metabolites. Objective. The aim of the research was to determine the role of RONS and accumulation of lipid peroxidation derivatives in initial development and formation of chronical inflammatory process in periodontium. Methods. Experimental periodontitis was modeled in animals by injection of complex mixtures of microorganisms diluted in egg protein into periodontal tissues. The results of biochemical studies of free radical processes activity in blood serum were evaluated by content of diene, triene conjugates, TBA-active products and total quantity of metabolites of nitric oxide (NO2–+NO3–), which were determined on the 7th, 14th and 30th days of the experiment. Results. Generation of active forms of oxygen is more influential, providing longevity of inflammatory process. This pays attention to typical dynamics of changes in active processes of lipid peroxidation in the development and course of experimental periodontitis. The study of inflammatory process with a bacterial-immune component in the rats’ periodontal complex proved accumulation of lipid peroxidation and nitric oxide metabolites in blood serum.Conclusions. The preservation of increased lipid peroxidation and nitric oxide metabolites in blood serum of the experimental animals with acute periodontitis conduce enhance of alteration and delayed healing that result in its sequel into chronical periodontitis.

scholarly journals Indicators of antioxidant protection of blood in necrotizing ulcerative gingivitis in experimental animals

2021 ◽  
Vol 14 (1) ◽  
pp. 68-74
Author(s):  
Yuliia Hafizivna Kilmukhametova ◽  
◽  
◽  
◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Catalase Activity ◽  
Local Treatment ◽  
Malonic Dialdehyde ◽  
Blood Parameters ◽  
Pathological Process ◽  
The State ◽  
Antioxidant Protection ◽  
Experimental Animals ◽  
Work Tasks

This article highlights the results of a study of blood parameters in animals with simulated necrotizing ulcerative gingivitis and compares them, under the same conditions, with animals that received local treatment with a developed complex of antioxidant drugs. Following the work tasks, the nature of changes in the state of the antioxidant - prooxidant system and their influence on quantitative and functional indicators of markers of inflammatory intensity was analyzed and investigated during the pathological process in the background and without treatment with a developed complex. This work shows the changes of malonic dialdehyde concentration as an indicator of lipid peroxidation intensity in experimental animals, the level of catalase activity in the blood of animals, and antioxidant-prooxidant balance in the dynamics of necrotizing ulcerative gingivitis.

scholarly journals Особливості клінічних проявів ураження тканин пародонта у підлітків на тлі аліментарно-конституціонального ожиріння

2017 ◽  
Author(s):  
O. I. Lebid ◽  
K. M. Duda
Keyword(s):  
Body Weight ◽  
Statistical Processing ◽  
Pathological Process ◽  
The State ◽  
Index Score ◽  
The Body ◽  
Control Group ◽  
Excess Body Weight ◽  
Clinical Indicators ◽  
Periodontal Tissues

The article presents the results of clinical examination of periodontal tissues in adolescents against the background of alimentary-constitutional obesity.The aim of the study – to investigate the features of the course of diseases of the periodontal tissue according to clinical indices in persons with alimentary-constitutional obesity.Materials and Methods. As a result of the survey, 95 adolescents aged 16–18 years old were included to study the features of the course of infl ammatory diseases of periodontal tissues in adolescents with alimentary-constitutional obesity 76 adolescents and 19 adolescents with harmonious physical development. To clarify the diagnosis, «excessive body weight» was determined by weight and height of the patient, and measured the circumference of the waist and hips. In the future, the body mass index (BMI) was calculated as the ratio of body weight in kg to square of growth in m2. Diagnosis of excess body weight was confi rmed with BMI less than 30kg/m2. The evaluation of the condition of periodontal tissues was carried out according to subjective and objective criteria (clinical parameters, index score). For the verifi cation of the pathological process in the periodontium, the classifi cation of M. F. Danilevskyi (1994) was used. The state of periodontal tissues was described using a traditional index score. Statistical processing of the results was carried out using commonly used methods of variation statistics with a help of personal computer using a statistical software package «Statistica 8.0» («Statsoft», USA). The probability level was estimated at 95 % (p<0.05) using Student’s t criterion.Conclusions. Consequently, the clinical indicators of the state of periodontal tissues in the context of alimentaryconstitutional obesity (Fedorov-Volodkin’s index, Green-Vermillion index, Sillness-Loe index, CPITN index) signifi cantly deteriorate compared with the corresponding indicators in the control group of adolescents without AKO, indicating the effect of alimentary-constitutional obesity on the clinical course, the severity and severity of theinfl ammatory process and the feasibility of timely and effective pharmacotherapy.

scholarly journals Make-Up Removers and Eye Surface

2021 ◽  
Vol 18 (1) ◽  
pp. 143-151
Author(s):  
V. N. Trubilin ◽  
E. G. Poluninа ◽  
K. V. Chinenova ◽  
V. V. Kurenkov ◽  
S. G. Kapkova
Keyword(s):  
Inflammatory Process ◽  
Clinical Signs ◽  
Subjective Assessment ◽  
Pathological Process ◽  
The State ◽  
Skin Type ◽  
Cosmetic Products ◽  
Schirmer Test ◽  
Chronic Inflammatory Process ◽  
Test Parameters

Purpose: to evaluate the influence on the state of the eye surface and the tear-producing system of decorative cosmetics and cosmetic products with different chemical bases for removing makeup.Patients and methods. The study included 45 (90 eyes) persons without clinical signs of an acute inflammatory process of the eye surface, they were prescribed the use of one of the Biore products: 15 people — makeup removal wipes, 15-micellar water, 15-makeup removal serum. The results of the objective (biomicroscopy, tests for lacrimal production) and subjective examinations are included in the questionnaires developed and standardized for this study (3 visits after the start of the use of cosmetics, 14, 30 days after the start of their use).Results. There were no significant differences (p > 0.5) between the groups that used makeup remover products on different bases — makeup remover serum, micellar water, makeup remover wipes — in all the patients included in the study. Patients with signs of a chronic inflammatory process on the eye surface and dry eye syndrome are less tolerant of makeup remover, which is manifested in a lower subjective assessment of these products after their use compared to patients without signs of an inflammatory process (p < 0.5). A correlation was determined at r = 0.3085, p = 0.0392 between the skin type and the Schirmer test parameters, exactly, the more oily the skin, the higher the Schirmer test parameters, it indicates a possible relationship between the skin type and the functional activity of the tear-forming glands, which indicates the need for further research in this area. The absence of a correlation (p > 0.5) between objective and subjective parameters that characterize the state of the eye surface and the tear-producing system, as well as the number of hours per day when decorative cosmetics are used, indicates that prolonged use of this type of exposure does not lead to the development of a pathological process on the eye surface.

scholarly journals Investigation of relationship between the level of cytokines and clinical parameters of patients with generalized periodontitis under the influence of Zinaxin

2014 ◽  
Vol 18 (3 (71)) ◽  
Author(s):  
S. S. Romanyshyn ◽  
V. I. Gereluk ◽  
O. P. Kobryn ◽  
N. I. Kukurudz ◽  
L. I. Kurbatova ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Pathological Process ◽  
Laboratory Research ◽  
Clinical Parameters ◽  
Clinical Indicators ◽  
Periodontal Tissues ◽  
Process Formation ◽  
Cytokine Imbalance ◽  
Pro Inflammatory Cytokines

As a result of a comprehensive clinical and laboratory research the pathogenetic role of cytokine imbalance in the pathological process formation in the periodontal tissues was established. Correlation analysis of cytokines’ content in gingival and oral fluid of patients with generalized periodontitis and data of clinical indicators of periodontal tissues showed that there is a strong direct connection between pro-inflammatory cytokines and clinical parameters.

scholarly journals Role of Lactate in Inflammatory Processes: Friend or Foe

2022 ◽  
Vol 12 ◽  
Author(s):  
Carolina Manosalva ◽  
John Quiroga ◽  
Alejandra I. Hidalgo ◽  
Pablo Alarcón ◽  
Nicolás Anseoleaga ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Inflammatory Mediator ◽  
Waste Product ◽  
Pathological Process ◽  
Extracellular Acidification ◽  
Post Translational Modifications ◽  
Transcription Factor Activation ◽  
Inflammatory Processes ◽  
G Protein Coupled

During an inflammatory process, shift in the cellular metabolism associated with an increase in extracellular acidification are well-known features. This pH drop in the inflamed tissue is largely attributed to the presence of lactate by an increase in glycolysis. In recent years, evidence has accumulated describing the role of lactate in inflammatory processes; however, there are differences as to whether lactate can currently be considered a pro- or anti-inflammatory mediator. Herein, we review these recent advances on the pleiotropic effects of lactate on the inflammatory process. Taken together, the evidence suggests that lactate could exert differential effects depending on the metabolic status, cell type in which the effects of lactate are studied, and the pathological process analyzed. Additionally, various targets, including post-translational modifications, G-protein coupled receptor and transcription factor activation such as NF-κB and HIF-1, allow lactate to modulate signaling pathways that control the expression of cytokines, chemokines, adhesion molecules, and several enzymes associated with immune response and metabolism. Altogether, this would explain its varied effects on inflammatory processes beyond its well-known role as a waste product of metabolism.

scholarly journals THE ROLE OF LEUKOCYTE PROTEINASES IN THE PATHOGENESIS OF INFLAMMATORY PERIODONTAL DISEASES IN PATIENTS WITH METABOLIC SYNDROME SIGNS

2020 ◽  
Vol 16 (1) ◽  
pp. 70-74
Author(s):  
Viktoriya Miroshnichenko ◽  
Alena Kokoreva ◽  
Anastasiya Safonova
Keyword(s):  
Pathogenic Bacteria ◽  
Inflammatory Process ◽  
Periodontal Diseases ◽  
Capillary Endothelium ◽  
Elastic Fibers ◽  
Bacterial Invasion ◽  
Tissue Destruction ◽  
Destructive Effect ◽  
Periodontal Tissues

Subject. Despite the constant efforts of practicing physicians and scientists, the prevalence proportion of inflammatory and destructive periodontal diseases in the world is not subsiding. Previously scientists have examined several different theories on the development of pathological conditions in periodontal tissues, ranging problems from the neurotrophic theory of occurrence to the effect of occlusion injury on periodontal tissues. Theories dealing with the influence of specific and non-specific bacterial plaque have also been studied. None of the theories has become reasonable and single valued. It is believed that the mechanism of the development of the disease is complex, autoimmune, damaging primarily collagen, elastic fibers of the capillary endothelium, gingival ligament and bone tissue. Over the past decade, it has been proven that inflammation in periodontal tissues is initiated and proceeds in response to the introduction of pathogenic bacteria. However, it can be said that not all patients develop an inflammatory response to bacterial invasion. The second important factor in the development and progression of the inflammatory-destructive process is the immune response of a particular individual. Scientists have noted the influence of the non-microbial factor and have come to the conclusion that in the surrounding tissues of the inflamed area there are not only enzymes secreted by bacteria, but also leukocyte proteinases, which in turn are mediators of the tissue destruction. Purpose ― to examine the role of leukocyte proteinases in the pathogenesis of inflammatory periodontal diseases. Methodology. The number of indigenous and foreign literature sources dealing with the study of the role of leukocyte proteinases in the development and maintenance of a destructive inflammatory process has been reviewed by us. Conclusion. The role of the aggressive destructive effect of leukocyte proteinases on periodontal tissues has been analyzed. Leukocyte proteinases are mediators of progressive destruction of connective tissue and can be used as markers to assess the severity of the course and predict the development of the inflammatory process in periodontal tissues.

scholarly journals CYTOKINE MECHANISMS FOR EARLY PERIOD OF EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS DEVELOPMENT

2018 ◽  
pp. 130-133
Author(s):  
A. Ye. Demkovych ◽  
Yu. I. Bondarenko ◽  
K. Ye. Yuriyiv
Keyword(s):  
Blood Serum ◽  
Inflammatory Reaction ◽  
Early Stage ◽  
Early Period ◽  
Pathological Process ◽  
Male Rats ◽  
Characteristic Change ◽  
Experimental Animals ◽  
Tnf Α ◽  
Experimental Periodontitis

The article presents the results of research on the parameters of cytokine profile as mediator of immune defense, which was determined by the level of proinflammatory and anti-inflammatory cytokines in blood serum of intact animals and on the 7th day of the experimental bacterial-immune periodontitis development. Attention is drawn to the characteristic change in the indicators of the cytokine link of immunity at the early stage of the development of the experimental periodontitis. Based on these results, it was proved that the formation of the pathological process in the periodontal complex is accompanied by an imbalance in the system of cytokinogenesis for the early period of the inflammatory reaction development, indicating their modulating role in the regulation of the immune-inflammatory response in this modeled pathological process. The aim of the study – to determine pathogenetic role of cytokinogenesis for early stages of the experimental bacterial-immune periodontitis development. Materials and Methods. The investigations was performed with use of white, non-breeding, clinically healthy male rats 150–200 g  of weight in conditions of vivarium. Experimental animals were randomly selected and divided into two experimental groups: the group 1 – intact animals, control; the group 2 – animals with an experimental periodontitis on the 7th day. Experimental bacterial-immune periodontitis in experimental animals was caused by introducing into the tissue of the periodontal complex a mixture of microorga­nisms diluted with egg protein [10]. In order to strengthen the immune response, an injection into the rat's foot of the full Freund's adjuvant was performed at the same time. For further research the blood serum was selected, in that was determined the content of tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-4 (IL-4), interleukin-10 (IL-10). Conclusion. Formation of experimental periodontitis is accompanied by a complex of pathological changes characterized by reliable progressive increase of proinflammatory cytokines for the early stage of inflammatory reaction development, that prove participation of TNF-α and IL-1β in the periodontal diseases pathogenesis.

scholarly journals Emerging Role of PPAR-β/δin Inflammatory Process Associated to Experimental Periodontitis

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Rosanna Di Paola ◽  
Francesco Briguglio ◽  
Irene Paterniti ◽  
Emanuela Mazzon ◽  
Giacomo Oteri ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Inos Expression ◽  
Peroxisome Proliferator ◽  
Sprague Dawley ◽  
Silk Thread ◽  
Peroxisome Proliferator Activated Receptor ◽  
Sprague Dawley Rats ◽  
Experimental Periodontitis ◽  
Mandibular First Molar

The aim of the present study was to evaluate the contribution of peroxisome proliferator-activated receptor (PPAR-β/δ) in animal model of periodontitis. Male Sprague-Dawley rats were lightly anaesthetized with pentobarbitone (35 mg/kg). Sterile, 2-0 black braided silk thread was placed around the cervix of the lower left first molar and knotted medially. Animals received GW0742 (0.3 mg/kg, 10% DMSO, i.p. after the ligature placement and daily for eight days). At day 8, the gingivomucosal tissue encircling the mandibular first molar was removed. One the eighth day after placement of the ligature, we evaluated (1) NF-κB expression, (2) cytokines expression, (3) iNOS expression, (5) the nitration of tyrosine, (6) apoptosis, and (8) the degree of gingivomucosal tissues injury. Administration of GW0742 significantly decreased all of the parameters of inflammation as described above. Taken together, these results demonstrate that GW0742 exerts an anti-inflammatory role during experimental periodontitis and is able to ameliorate the tissue damage.

scholarly journals REGULATORY INFLUENCE OF BLOOD MONOCYTES ON THE POPULATION COMPOSITION OF GRANULOCYTES AND THE STATE OF THEIR RESPIRATORY BURST IN THE WIDESPREAD PURULENT PERITONITIS

2018 ◽  
Vol 8 (2) ◽  
pp. 201-210
Author(s):  
A. A. Savchenko ◽  
A. G. Borisov ◽  
D. V. Cherdancev ◽  
O. V. Pervova ◽  
I. V. Kudryavcev ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Peripheral Blood ◽  
Inflammatory Process ◽  
The State ◽  
Regulatory Effect ◽  
Population Composition ◽  
Hla Dr ◽  
Purulent Peritonitis ◽  
Classical Monocytes

The aim of the study was to investigate the regulatory effect of monocytes and their subpopulations on the population composition  of granulocyte leukocytes and the state of their respiratory burst in  widespread purulent peritonitis (WPP). The study involved 24  patients aged 30-65 with acute surgical diseases and injuries of  abdominal organs complicated by WPP. As a control 25 relatively  healthy people of the same age range were examined. A study of the population composition of monocytes and granulocyte leukocytes in  blood was performed using a two-platform technology on the  hematological analyzer Sysmex XE-5000 (Sysmex Inc., USA) and  FC-500 flow cytometer (Beckman Coulter, USA) using the Cytodiff antibody kit (Beckman Coulter, USA). A study of the  monocytes number expressing HLA-DR- and CD64-receptor was performed by flow cytometry using direct immunofluorescence  of whole peripheral blood. The respiratory burst state of neutrophilic granulocytes was studied by chemiluminescence analysis on a 36-channel chemiluminescence analyzer BLM-3607 (MedBioTech, Russia). As indicators of chemiluminescence  were used luminol and lucigenin. The enhancement of  chemiluminescence induced by zymosan was evaluated by the ratio  of the area of the induced chemiluminescence to the spontaneous area and was defined as the activation index. It has  been established that the immune-inflammatory process in WPP is  characterized by a decrease in the number of classical monocytes in  the peripheral blood and an increase in the content of non-classical  monocytes. In WPP in peripheral blood the level of monocytes  expressing HLA-DR receptors decreases. The change in the ratio of  monocytes subpopulations characterizes the increase in the role of  the proinflammatory fraction in the WPP pathogenesis. Changes in  the population composition of granulocytes in the blood in patients  with WPP also characterize the development of an acute  inflammatory process. In this case, there is a decrease in the  number of basophils in the peripheral blood, which, apparently, is  determined by the presence of an allergic component in WPP and,  accordingly, their migration to the inflammation area. In patients  with WPP activation of a respiratory burst of granulocytes of blood  was detected, the intensity of which is determined by the synthesis  of primary and secondary active oxygen species. The results of the  correlation analysis made it possible to establish that in WPP the  regulatory role of non-classical monocytes increases aimed at  stimulating the inflammatory processes (an increase in the number  of mature and immature forms of neutrophils and stimulation of the activity of a respiratory explosion of granulocytes). The revealed features of the regulatory effect of monocytes on the population  composition and the intensity of the respiratory burst of granulocytes can be used in the development of immunotherapeutic methods aimed at reducing the activity of the inflammatory process in WPP.

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