scholarly journals Antioxidant activity of the fractions from water lettuce (Pistia stratiotes) extract

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 451-455
Author(s):  
Herpandi ◽  
S.D. Lestari ◽  
Bastian ◽  
S. Sudirman
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Functional Groups ◽  
Column Chromatography ◽  
Human Body ◽  
Pistia Stratiotes ◽  
Hydroxyl Group ◽  
Water Lettuce ◽  
The Third ◽  
Ft Ir

Free radicals including reactive oxygen species are continuously increasing in the human body. This condition causes the unbalance between free radicals and antioxidants in the human body. An antioxidant is a compound with the ability to reduce the harmfulness of the free radical. This study aimed to determine the antioxidant activity of fractions and analyzed the functional groups of water lettuce (Pistia stratiotes) methanol extract. The separation process was performed by using thin-layer chromatography (TLC) and column chromatography. The separated fractions were measured for their antioxidant activity by using the 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The functional groups of each fraction were determined by using Fourier-transform infrared (FT-IR) spectroscopy. The separation of water lettuce extract by using column chromatography produced seven fractions with different colors and confirmed by using TLC. The antioxidant activity showed the highest activity in the third fraction with a half-maximal inhibitory concentration (IC50) value of 131.66 ppm. The fifth fraction with the IC50 was about 184.62 ppm. Whereas, the first, second, fourth, sixth, and seventh fractions were relatively weak with the IC50 more than 200 ppm. The FT-IR spectrum also showed that the intensity hydroxyl group in the third fraction higher than the seventh fraction.

scholarly journals Antioxidant Activity of Medicinal Plants from Habitats with Different Anthropogenic Loads

2020 ◽  
Vol 6 (2) ◽  
pp. 119-127
Author(s):  
S.A. Kurbatov ◽  
◽  
V.M. Zubkova ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Human Body ◽  
Raw Materials ◽  
Mechanism Of Formation ◽  
Analysis Of Results ◽  
Biochemical Research ◽  
First Time

the analysis of results of biochemical research concerning antioxidant activity of medicinal raw materials, with use of a method of inhibition of autooxidation of adrenaline in vitro is carried out. The mechanism of formation of free radicals and their sources is considered. A brief overview of the action of free radicals on the human body and cells is given. For the first time, depending on the biological characteristics, the antioxidant activity of plants in areas characterized by different anthropogenic loads was studied: an increase in the anthropogenic load was accompanied by a decrease in the antioxidant activity of plants.

Invivo Protective Effect of Indigofera on Hepatic Tissue

2020 ◽  
Vol 8 (1) ◽  
pp. 7-10
Author(s):  
Vishal N Kushare ◽  
Suvarna A Wagh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Body ◽  
Ethanol Extract ◽  
The Body ◽  
Hepatic Tissue ◽  
Cell Organelle ◽  
The World ◽  
Ethanol Extracts

Oxidative Stress is the primary cause of most of the diseases currently in the world. The human body is prone to many illnesses out of which oxidative stress plays a significant role in the causation of those. The Stress in the body releases free radicals and these free radicals react with the cell organelle and cause denaturation and break down. The generated free radicals should be fought to eliminate and prevent toxic effects. Many drugs are known to fight free radicals. Most of them had been scientifically evaluated and published for their activity too. The investigations say that antioxidant activity of the herbs does not similarly reflect . There were differences in activity and significant deviations in the results that are shown in comparison to and . Thus, there is an urgent necessity to investigate the antioxidant activity of herbs to make sure that the activity resembles and to find any variations when compared to activity. This research protocol was framed out to screen for the antioxidant activity of the ethanol extracts of the Indigofera leaves by estimating the serum parameters and correlating the results with the human body. Out of the values overall, the were the ones that are significant participants in fighting the free radicals and in boosting the immune system. The ethanol extract successfully prevented the breakdown of the cellular structure of the liver cells, thereby protecting it and replenishing the enzyme levels by destroying the free radicals that are generated due to DMH.

Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.

2011 ◽  
Vol 236-238 ◽  
pp. 2114-2117
Author(s):  
Ming Ye ◽  
Ru Yue Yuan ◽  
Tao Qiu ◽  
Jing Min Cai
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Column Chromatography ◽  
Raw Material ◽  
Homogeneous Component ◽  
Extraction Time ◽  
Microwave Method ◽  
Optimal Conditions ◽  
Orthogonal Experiments

The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.

scholarly journals Basil – a comparison of the total phenolic content and antioxidant activity in selected cultivars

10.5219/1623 ◽  
2021 ◽  
Vol 15 ◽  
pp. 445-452
Author(s):  
Martin Adámek ◽  
Jiří Mlček ◽  
Anna Adámková ◽  
Vít Guiglielmo Mišurec ◽  
Jana Orsavová ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Body ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Content ◽  
Total Phenolic ◽  
Cold Temperatures ◽  
Significant Difference ◽  
Phenolic Substances

During the lifetime, the human body forms a considerable amount of free radicals damaging DNA, cell membranes, and their components. A wider application of basil (Ocimum basilicum), an aromatic plant and one of the common gastronomic commodities in the human diet could help to prevent the formation of free radicals and to remove them from the human body. Therefore, determination of antioxidant activity and total content of phenolic substances in selected cultivars of basil (Ohře, Sweet green, Salad leaf, Purple opal, Thai) in a fresh and frozen state and the mixture with another plant (garlic, mint, rocket, spinach) in the fresh and refrigerated state was performed. The total content of phenolic substances in basil was established by the spectrophotometric Folin-Ciocalteu method. DPPH spectrophotometric method was used to analyze the antioxidant activity. The results have shown statistically significant differences between basil cultivars under the same growing conditions. The values of antioxidant activity in frozen samples ranged from 5.1 ±0.4 mg.g-1 AA FW to 11.71 ±0.18 mg.g-1 AA FW and the total phenolic content varied between 2.77 ±0.16 mg.g-1 GAE FW in TH and 8.93 ±0.13 mg.g-1 GAE FW. A statistically significant difference between fresh and frozen samples was established only in the “Ohře” cultivar. After the storage in cold temperatures, all mixtures showed a reduction in the antioxidant activity and total content of phenolic substances. The mixture of basil and mint performed the highest values of antioxidant activity and total content of phenolic substances and significantly differed from the other mixtures most often. The addition of basil and its mixtures to food and beverages can substantially increase their biological value and subsequently also the quality of human nutrition.

scholarly journals Use of Yoghurt Enhanced with Volatile Plant Oils Encapsulated in Sodium Alginate to Increase the Human Body’s Immunity in the Present Fight Against Stress

2020 ◽  
Vol 17 (20) ◽  
pp. 7588
Author(s):  
Ovidiu Tița ◽  
Maria Adelina Constantinescu ◽  
Mihaela Adriana Tița ◽  
Cecilia Georgescu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Sodium Alginate ◽  
Human Body ◽  
Raw Materials ◽  
Stress Factors ◽  
Cow Milk ◽  
Aromatic Plants ◽  
Volatile Oils

(1) Background: The COVID–19 pandemic and the imposition of strict but necessary measures to prevent the spread of the new coronavirus have been, and still are, major stress factors for adults, children, and adolescents. Stress harms human health as it creates free radicals in the human body. According to various recent studies, volatile oils from various aromatic plants have a high content of antioxidants and antimicrobial compounds. An external supply of antioxidants is required to destroy these free radicals. The main purpose of this paper is to create a yoghurt with high antioxidant capacity, using only raw materials from Romania; (2) Methods: The bioactive components used to enrich the cow milk yoghurt were extracted as volatile oils out of four aromatic plants: basil, mint, lavender and fennel. Initially, the compounds were extracted to determine the antioxidant capacity, and subsequently, the antioxidant activity of the yoghurt was determined. The 2,2-diphenyl-1-picrylhy-drazyl (DPPH) method was used to determine the antioxidant activity; (3) Results: The results show that cow milk yoghurt enhanced with volatile oils of basil, lavender, mint and fennel, encapsulated in sodium alginate has an antioxidant and antimicrobial effect as a staple food with multiple effects in increasing the body’s immunity. The antioxidant activity proved to be considerably higher than the control sample. The highest antioxidant activity was obtained on the first day of the analysis, decreasing onwards to measurements taken on days 10 and 20. The cow milk yoghurt enriched with volatile basil oil obtained the best results; (4) Conclusions: The paper shows that yoghurts with a high antioxidant capacity were obtained, using only raw materials from Romania. A healthy diet, compliance with safety conditions and finding appropriate and safe methods to increase the body’s immunity is a good alternative to a major transition through harder times, such as pandemics. The creation of food products that include natural antioxidant compounds combines both the current great possibility of developing food production in Romania and the prevention and reduction of the effects caused by pandemic stress in the human body.

scholarly journals Potensi Ekstrak Etanol Herba Apu-Apu (Pistia stratiotes) dan Fraksi-Fraksinya Sebagai Antioksidan Dengan Metode DPPH

2019 ◽  
Vol 16 (2) ◽  
pp. 83-88
Author(s):  
Dewi Dianasari ◽  
Irawati Firdiyansari
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Herbal Extract ◽  
Pistia Stratiotes ◽  
Water Fraction ◽  
Dpph Method

Free radicals play an important role in cell damage and degenerative diseases such as coronary heart disease, stroke, premature aging and others. Compounds that can inhibit free radicals are antioxidants. One of the plants that has a high phenolic compound and can be a source of natural antioxidants, apu-apu or Pistia stratiotes. This research was conducted to scientifically prove the antioxidant activity of the apu-apu herbal extract (Pistia stratiotes) and its fractions by the DPPH method. The sample used in the form of all parts apu-apu (apu-apu herb) obtained from the Sumbersari District, Jember Regency. In this study the DPPH method was used with a DPPH concentration of 0.1 mM, incubation time of ethanol extract 40 minutes, fraction of n-hexane 50 minutes, ethyl acetate fraction 45 minutes, ethanol-water fraction 40 minutes,vitamin C 35 minutes and measured at wavelength 517 nm . The results of the average antioxidant activity (IC50) were apu-apu herbal extract 16.675 µg / mL ± 0.239, n-hexane fraction 29.915 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038, ethanol-water fraction 9.090 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038, ethanol-water fraction 9.090 µg / mL ± 0.156, ethyl acetate fraction 11.875 µg / mL ± 0.038 / mL ± 0.122, and vitamin C 3.263 µg / mL ± 0.032. Vitamin C as a positive control had the highest activity, whereas the apu-apu sample which had the highest antioxidant activity was the ethanol-water fraction, and the lowest was the n-hexane fraction. The conclusion of this study is apu-apu herbal ethanol extract and its fractions have antioxidant activity, from high to low sequentially: ethanol-water fraction, ethyl acetate fraction, apu-apu herbaceous fraction, n- fraction hexane.

scholarly journals Synthesis and primary screening of the antioxidant activity of some 4-thioxo- and 4-imino- thiazolidin-2-ones

2021 ◽  
pp. 17-25
Author(s):  
Z. І. Сhulovska ◽  
І. V. Drapak ◽  
T. I. Chaban ◽  
V. S. Matiychuk ◽  
I. G. Chaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant System ◽  
Human Body ◽  
Structural Modification ◽  
High Efficiency ◽  
Antioxidant Effect ◽  
Primary Screening ◽  
Derivatives Of

As a result of metabolic transformations of substances in the human body in the reactions of lipid peroxidation, reactive oxygen species are formed. Normally, the regulation of the production of activated oxygen metabolites and free radicals in human tissues and organs is carried out by the antioxidant system. Despite its high efficiency, antioxidant system it is not always to protect the human body from the development of oxidative stress. Thiazolidinones are among the most intensively studied heterocyclic systems, since they possess a wide spectrum of biological activity. Based on the above, the synthesis of some 4-thioxo- and 4-iminothiazolidin-2-ones and the study of their antioxidant activity is an topical direction. The aim of our work was to synthesize some C5 substituted derivatives of 4-thioxothiazolidin-2-one and 4-iminothiazolidin-2-one, as well as to conduct a primary screening of their antioxidant activity. The objects of study were C5 substituted 4-thioxo- and 4-iminothiazolidin-2-ones. The composition and structure of the synthesized compounds were confirmed by the data of elemental analysis and 1H NMR spectroscopy. The antioxidant activity of the synthesized compounds was studied in vitro, determining the decrease in the concentration of free radicals. The synthesis of 12 derivatives of 4-thioxothiazolidin-2-one and 4-iminothiazolidin-2-one was carried out. These substances are obtained by structural modification  to the C5 position of the base scaffolds. The antioxidant activity of the synthesized compounds was measured in vitro by the method of scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Ascorbic acid was used as a standard. As a result of screening, 2 highly active compounds with a pronounced antioxidant effect were identified. As a result of structural modification of the basic scafolds, 12 corresponding C5 substituted thiazolidinones were obtained. During the primary pharmacological screening of antioxidant activity, 2 substances with a pronounced antioxidant effect were isolated. Further optimization of the structure is currently in progress.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Antioxidant Activity of the Isoflavonoids from the Roots of Maackia amurensis

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Nadezda I. Kulesh ◽  
Sergey A. Fedoreyev ◽  
Marina V. Veselova ◽  
Natalia P. Mischenko ◽  
Vladimir A. Denisenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Column Chromatography ◽  
Thiobarbituric Acid ◽  
Formalin Injection ◽  
Thiobarbituric Acid Reactive Substances ◽  
H Nmr ◽  
Maackia Amurensis

Seven isoflavonoids, including a new glycoside, (6a R,11a R)-medicarpin-3- O-gentiobioside (6), were isolated from the roots of Maackia amurensis using repeated column chromatography on a Toyopearl HW-50F sorbent and identified by HPLC–PDA–MS, 1H NMR, 13C, 1H–1H COSY, HSQC NMR and HMBC NMR analyses as daidzin (1), genistein-7- O-gentiobioside (2), pseudobaptigenin-7- O-gentiobioside (3), formononetin-7- O-gentiobioside (4), (6a R,11a R)-maackiain-3- O-gentiobioside (5), and 5- O-methylgenistein-7- O-gentiobioside (7). In the model of oxidative stress induced by formalin injection, the isolated isoflavone and pterocarpan glucosides 1-7 were shown to reduce the formation of malondialdehyde (MDA) and other thiobarbituric acid reactive substances (TBARS), as well as glutathione peroxidase (GPO) activity in rats.

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

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