Uji efektivitas gel ekstrak etanol daun kelor (Moringa oleifera L.)  sebagai antijamur Malassezia furfur

Abstrak Kandungan daun kelor (Moringa oleifera L) diketahui dapat berkhasiat sebagai antijamur. Penelitian ini bertujuan untuk memformulasi dan mengetahui aktivitas antijamur gel yang mengandung daun kelor terhadap jamur Malasezia furfur. Ekstraksi daun kelor dilakukan menggunakan etanol 70% dengan metode maserasi. Formulasi gel yang mengandung ekstrak etanol daun kelor dilakukan menggunakan hidroksi propil metil selulosa (HPMC) dengan berbagai konsentrasi, yaitu 2, 3, dan 4%., Evaluasi gel yang dilakukan adalah uji organoleptik, homogenitas, pH, daya sebar, daya lekat, dan viskositas. Kemudian dilakukan uji aktivitas antijamur dengan menggunakan metode difusi padat dengan kertas cakram, dan digunakan gel ketokonazol sebagai pembanding. Parameter yang diukur adalah zona hambat antijamur. Hasil kemudian dianalisis statistik Kruskall Wallis dan Mann Withney. Berdasarkan evaluasi gel, diperoleh bahwa peningkatan konsentrasi basis HPMC 2, 3, dan 4% dalam sediaan gel ekstrak etanol daun kelor dapat meningkatkan viskositas gel, daya lekat gel, menyebabkan penurunan daya sebar gel, dan mempengaruhi peningkatan konsistensi sediaan gelpada uji organoleptik tanpa mempengaruhi uji pH gel dan homogenitas gel. Formula yang memiliki sifat fisik gel yang yang baik jika dilihat dari hasil uji sifat fisik gel yang meliputi uji organoleptik,homogenitas, pH, daya sebar daya lekat dan viskositas yaitu formula gel ekstrak etanol daun kelor dengan konsentrasi basis HPMC 2 % karena hampir dari semua uji memenuhi persyaratan literatur. Dari penelitian ini dapat disimpulkan gel ekstrak etanol daun kelor mempunyai aktivitas antijamur M.furfur dan gel yang mengandung HPMC 2% memiliki sifat fisik gel dan aktivitas antijamur lebih baik jika dibandingkan dengan gel konsentrasi HPMC 3 dan 4%. Kata Kunci: Daun Kelor, Moringa oleifera L, gel, antijamur, Malassezia furfur. The effectiveness test of gel of ethanol extract of Moringa oleifera leaves as antifungal of Malassezia furfur Abstract Moringa leaf content (Moringa oleifera L) is known to be efficacious as an antifungal. The aim of this research is to formulate and to know gel antifungal activity containing moringa leaf against Malasezia furfur fungus. Moringa leaf extraction was done using 70% ethanol by maceration method. The gel formulation containing ethanol extract of moringa was conducted using hydroxy propyl methyl cellulose (HPMC) with various concentrations, ie 2, 3, and 4%. Evaluation of gel performed was organoleptic, homogeneity, pH, spreading, and viscosity. Then the antifungal activity was tested using solid diffusion method with paper disc, and used ketoconazole gel as comparison. The measured parameter was the antifungal block zone. The results were then analyzed statistically using Kruskall Wallis and Mann Withney. Based on the gel evaluation, it was found that the increased concentrations of HPMC 2, 3, and 4% base concentrations in the gel preparation of ethanol extract of moringa leaf can increase gel viscosity, gel adhesiveness, decrease the gel power, and influence the consistency of preparation for organoleptic test without affecting the test pH gel and gel homogeneity. Formula which have good physical properties of gel when viewed from the test results of physical properties of gel which include organoleptic test, homogeneity, pH, viscosity sticky power and viscosity is gel gel ethanol extract leaf kelor with 2% HPMC base concentration because almost of all test meet the literature requirements. From this study, it can be concluded that leaf extract gel ethanol gel has antifungal activity of M.furfur and gel containing HPMC 2% has gel physical properties and antifungal activity is better when compared with HPMC 3 and 4% concentration gel Keywords: Moringa olifera L.,gel, antifungal, Malasezia furfur

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Uji efektivitas gel ekstrak etanol daun kelor (Moringa oleifera L.) sebagai antijamur Malassezia furfur

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.119 ◽

2017 ◽

Vol 5 (2) ◽

pp. 62

Author(s):

Anna L Yusuf ◽

Ecin Nurawaliah ◽

Nurhidayati Harun

Keyword(s):

Antifungal Activity ◽

Physical Properties ◽

Moringa Oleifera ◽

Methyl Cellulose ◽

Ethanol Extract ◽

Diffusion Method ◽

Measured Parameter ◽

Malassezia Furfur ◽

Organoleptic Test ◽

Solid Diffusion

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ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

Collaborative Medical Journal (CMJ) ◽

10.36341/cmj.v3i2.1302 ◽

2020 ◽

Vol 3 (2) ◽

pp. 76-81

Author(s):

Rini Lestari

Keyword(s):

Antifungal Activity ◽

Secondary Metabolites ◽

Ethanol Extract ◽

Average Concentration ◽

Diffusion Method ◽

Microsporum Canis ◽

Malassezia Furfur ◽

Agar Diffusion Method ◽

Alpinia Purpurata ◽

Anti Fungal

Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

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A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7302 ◽

2018 ◽

Vol 7 (3) ◽

pp. 230-241

Author(s):

Savita Joshi ◽

◽

Parikshit Kumar ◽

Prabha Pant ◽

SC Sati ◽

...

Keyword(s):

Antifungal Activity ◽

Fungal Pathogens ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Total Activity ◽

Maximum Activity ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Cedrus Deodara ◽

Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

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OPTIMASI KONSENTRASI POLIVINIL PIROLIDON (PVP) SEBAGAI BAHAN PENGIKAT TEHADAP SIFAT FISIK TABLET EKSTRAK ETANOL RIMPANG BANGLE (Zingiber cassumunar Roxb)

Jurnal Farmasi Udayana ◽

10.24843/jfu.2018.v07.i02.p02 ◽

2018 ◽

pp. 45

Author(s):

Ida ayu Sintha Devi

Keyword(s):

Physical Properties ◽

Bitter Taste ◽

Ethanol Extract ◽

Wet Granulation ◽

Disintegration Time ◽

Organoleptic Test ◽

Size Uniformity ◽

The Right ◽

Test Requirements ◽

Zingiber Cassumunar

Zingiber cassumunar Roxb contains active compounds such as cassumumin AC, cassummarin AC, phenylbutanoid, and (E) -4- (3,4-dimethoxyphenyl) but-3-en-1-ol which has activities as anti-inflammatory, antioxidant and anti-allergenic agents so that they are potentially used as anti-allergies. Zingiber cassumunar Roxb is made into tablet because it has several advantages, which are easy to consume, practical, the right amount, cover the bitter taste and the distinctive smell and its stability is maintained in storage. This study aims to produce Zingiber cassumunar Roxb tablets that have good physical properties by using PVP as a binder. Zingiber cassumunar Roxb extract was made by maceration using 70% ethanol solvent, the tablet was formulated using wet granulation method. Zingiber cassumunar Roxb ethanol extract tablet was formulated in 3 formulas with variations in the PVP FI content of 1%, FII 2.75%, and FIII 4.5%. The resulting granules were tested for physical properties including moisture test, particle size distribution, flow time, stationary angle, bulk density, and compressibility, while the tablets produced were tested for physical properties including organoleptic test, uniformity of size, uniformity of weight, friability, and disintegration time. The results showed that the optimum concentration obtained was at FIII with 4.5% PVP concentration that complied the organoleptic test requirements, uniformity of size, weight uniformity, and fragility of tablets.

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Evaluation of Antibacterial and Antifungal Activity of Moringa Concanensis

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.4.6 ◽

2021 ◽

Vol 14 (4) ◽

pp. 5566-5570

Author(s):

Bindu Singh Yadav ◽

Yeddu Trilochana ◽

Kiran Kumari ◽

Saurabh Singh ◽

Anupam K Sachan

Keyword(s):

Antifungal Activity ◽

Bacterial Infections ◽

Methyl Cellulose ◽

Enterobacter Aerogenes ◽

Chloroform Extract ◽

High Rate ◽

Diffusion Method ◽

Significant Activity ◽

Antibacterial And Antifungal Activity ◽

Antibacterial And Antifungal

In today’s scenario, fungal and bacterial infections are one of the most challenging pestilential diseases for the health-care professional which is increasing with a very high rate in the growing population, especially among the immune compromised people.The present work involved the study to extract active principles of Moringa concanensis and perform antibacterial and antifungal activity for various extracts. The leaves were dried powdered unexpected using solvents petroleum ether, chloroform, ethanol and water. Chemical tests were performed to ascertain presence of various classes of phyto-constituents like alkaloids, glycosides, saponins, carbohydrates, proteins, amino acids tannins, flavonoids and phenolic substances. All four extracts showed the presence of various phytochemicals responsible for multiple pharmacological activities. The extracts were reconstituted using 1% aqueous solution of carboxy methyl cellulose and were tested for antibacterial and antifungal activity against six microorganisms, namely Bacillus subtilis, Enterobacter aerogenes, Bacillus cereus, Aspergillus Flavus, Aspergillus Niger and Helminthosporium, using well diffusion method. The results of the activity are promising with chloroform extract showing highest inhibitory activity against most of the organisms. All the extracts were able to demonstrate significant activity compared to control inhibition of microorganisms tested and hold key to discover lead molecules after further scientific investigation.

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Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans

WARTA FARMASI ◽

10.46356/wfarmasi.v8i2.117 ◽

2019 ◽

Vol 8 (2) ◽

pp. 71-80

Author(s):

Ummu Kalsum ◽

Ayu Ayu

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Daucus Carota ◽

Ethanol Extract ◽

Diffusion Method ◽

Daucus Carota L ◽

Zone Diameter ◽

Extract Concentration ◽

Activity Test ◽

Agar Diffusion Method

ABSTRAK Telah dilakukan penelitian Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol umbi wortel sebagai antifungi terhadap pertumbuhan Candida albicans dengan menggunakan variasi konsentrasi yang berbeda yaitu 1% b/v, 3% b/v dan 5% b/v. Ekstrak etanol umbi wortel diperoleh dengan cara maserasi menggunakan pelarut etanol 70%. Pengujian aktivitas antifungi menggunakan metode difusi agar dengan cara sumuran untuk mengetahui aktivitas antifungi dengan mengamati daerah hambatan. Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak 1% b/v, 3% b/v dan 5% b/v dapat memberikan aktivitas yang menghambat pertumbuhan jamur uji. Terdapat penambahan diameter zona hambat pada setiap kenaikan konsentrasi yaitu 1% b/v (15,7 mm), 3% b/v (18,9 mm), dan 5% b/v (19,8 mm) terhadap jamur Candida albicans. Semua variasi konsentrasi ekstrak memiliki aktivitas antifungi yang kuat dalam menghambat pertumbuhan Candida albicans. Kata kunci: Ekstrak, Daucus carota L., Antifungi, Candida albicans ABSTRACT It has conducted a study of activity test of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans. This the study aims at knowing the activity of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans by using different variance concentration they are 1% b/v, 3% b/v and 5% b/v. Carrot tuber ethanol the extract was obtained by maceration by using 70% of ethanol dissolver. Activity test of antifungal by using agar diffusion method with a well method to find out the the activity of antifungal by observing the obstruction zone. The result of this research shows that concentration extract 1% b/v, 3% b/v and 5% b/v can give activity which impedes the growth of fungal test. There is increasing of obstruction zone diameter in every increment of of the concentration they are 1% b/v (15,7 mm), 3% b/v (18,9 mm) and 5% b/v (19,8mm) toward Candida albicans fungi. All extract concentration variation has a strong antifungal activity in impeding the growth of Candida albicans.

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Antifungal resistance modulation of Aspergillus fumigatus isolates from brooder pneumonia affected birds by Eucalyptus globulus extracts

Abasyn Journal Life Sciences ◽

10.34091/ajls.3.2.14 ◽

2020 ◽

pp. 145-155

Keyword(s):

Antifungal Activity ◽

Aspergillus Fumigatus ◽

Eucalyptus Globulus ◽

Ethanol Extract ◽

Diffusion Method ◽

Broth Microdilution ◽

Diffusion Test ◽

Base Pairs ◽

Microdilution Method ◽

Broth Microdilution Method

Brooder pneumonia is a common respiratory problem during brooding period of poultry. It is most frequently caused by Aspergillus fumigatus. Use of azole drugs for treatment and prophylaxis results in resistance. The aim of this study was to determine whether the phytochemicals of Eucalyptus globulus are modulating resistance in Aspergillus fumigatus. Lung samples n = 50 of dead broiler chicks were collected from different farms of Lahore. Out of 50 lung samples 28 % (14) were considered positive for Aspergillus fumigatus after observing their macroscopic, microscopic characteristics and by performing PCR by amplifying ITS1-ITS4 gene (597 base pairs) and RodA gene (313 base pairs). Kirby-Bauer disc diffusion test was performed 14 isolates (100 %) were resistant towards both Fluconazole and Ketoconazole whereas 11 (78.57 %) were resistant towards Itraconazole. Eucalyptus globulus leaves were collected, and these were identified. Three solvent extracts were prepared. Maximum yield was of ethanolic extract. Antifungal activity was evaluated by agar well diffusion method. Highest antifungal activity was shown by Eucalyptus globulus Ethanol followed by chloroform and hexane extract showed no activity against any isolate then minimum inhibitory concentration of plant extracts that previously showed antifungal activity against isolates were evaluated by broth microdilution method. Modulation effect was checked by combining antifungal drug one by one with subinhibitory concentration of plant extract evaluated previously by broth microdilution method. 5 isolates were subjected into this experiment which are all sensitive towards Eucalyptus globulus ethanol and chloroform extracts tested previously in agar well diffusion test and MIC values were also evaluated. It was concluded that when Itraconazole was combined with E. globulus Ethanol extract then resistance was modified. Data of modulation was analyzed by One-way ANOVA and it reported P value of <0.05. It was synergistic inhibitory effect when Itraconazole was combined with Eucalyptus globulus Ethanol extract.

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Formulasi Sediaan Salep Esktrak Etanol Daun Sirsak (Anonna mucirata L.) Dengan Variasi Basis Salep

GLOBAL HEALTH SCIENCE (GHS) ◽

10.33846/ghs5303 ◽

2020 ◽

Vol 5 (3) ◽

pp. 106

Author(s):

Indian Cahyani ◽

Aulia D. Pelu ◽

Jayanti Djarami ◽

Epi Dusra

Keyword(s):

Physical Properties ◽

Traditional Medicine ◽

Dosage Form ◽

Ethanol Extract ◽

Water Soluble ◽

Homogeneity Test ◽

Organoleptic Test ◽

Ointment Base ◽

Ointment Formulation ◽

Soluble Base

The use of natural ingredients as traditional medicine with better use is now more in demand. This is because traditional medicine is relatively easy to obtain. One type of medicinal plant that is often used by the community is (Annona mucirata L). or better known as soursop. Soursop leaves (Anonna mucirata L.) have been widely used as a medicinal ingredient because it contains tannin and flavanoid compounds which can provide antibacterial effects. The purpose of this study was to determine the content of the compounds contained in soursop leaves (Anonna mucirata L.) and to formulate an ethanol extract ointment of soursop leaves (Anonna mucirata L.) with various ointment bases. This research was conducted using an experimental method in which evaluations of the ointment were carried out including the organoleptic test, pH test and homogeneity test. The results showed that ethanol extract of soursop leaves (Anonna mucirata L.) containing tannins and flavonoids. The results of the ointment formulation on the hydrocarbon base formulation, the absorption base formulation and the water soluble base formulation resulted in physical differences in the organoleptic test, but did not have different results on the homogeneity test and pH test. In conclusion, extract ethanol of soursop (Anonna mucirata L.) leaf contains tannin and flavanoid compounds. The ethanol extract ointment of soursop leaves (Anonna mucirata L.) with variations of FI (hydrocarbon base) and FII (absorption base) ointment has good ointment properties, while FIII (water-soluble base) does not have good physical properties because it has a dosage form which is liquid and smells rancid based on the organoleptic test. The ethanol extract ointment of soursop leaves (Anonna mucirata L.) with variations of FI (hydrocarbon base) and FII (absorption base) ointment has good ointment properties, while FIII (water-soluble base) does not have good physical properties because it has a liquid dosage form and smells rancid based on the organoleptic test. Keywords: ointment extract; soursop plant; ointment base ABSTRAK Pemanfaatan bahan alam sebagai obat tradisional dengan penggunaan yang lebih baik sekarang lebih diminati. Hal ini dikarenkan obat tradisional relatif mudah didapat. Salah satu jenis tanaman obat yang sering dimanfaatkan oleh masyarakat adalah (Annona mucirata L). atau yang lebih di kenal dengan nama sirsak. Tanaman daun sirsak (Anonna mucirata L.) sudah banyak digunakan sebagai bahan obat karena mengandung senyawa tannin dan flavanoid yang mampu memberikan efek antibakteri. Tujuan penelitian ini untuk mengetahui kandungan senyawa yang terdapat pada daun sirsak (Anonna mucirata L.) serta membuat formulasi sediaan salep esktrak etanol daun sirsak (Anonna mucirata L.) dengan variasi basis salep. Penelitian ini dilakukan dengan menggunakan metode experimental dimana dilakukan evaluasi terhadap salep yang dibuat meliputi uji organoleptik, uji pH dan uji homogenitas. Hasil penelitian ini yaitu ekstrak etanol daun sirsak (Anonna mucirata L.) mengandung senyawa tannin dan flavanoid, untuk hasil formulasi sediaan salep pada formulasi basis hidrokarbon, formulasi basis absorbsi dan formulasi basis larut air menghasilkan perbedaan fisik uji organoleptik, namun tidak memiliki hasil yang berbeda pada uji homogenitas dan uji pH. Dari hasil penelitian dapat disimpulkan yaitu ekstrak estanol daun sirsak (Anonna mucirata L.) mengandung senyawa tannin dan flavanoid. Sediaan salep esktrak etanol daun sirsak (Anonna mucirata L.) dengan variasi basis salep FI (basis hidrokarbon) dan FII (basis absorbsi) memiliki sifat salep yang baik, sedangkan FIII (basis larut air) tidak memiliki sifak fisik yang baik karena memiliki bentuk sediaan yang cair dan bau tengik berdasarkan uji organoleptik. Kata kunci: ekstrak salep; tanaman daun sirsak; basis salep

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AKTIVITAS ANTIJAMUR EKSTRAK ETANOL 50% DAN ETANOL 96% DAUN PACAR KUKU Lawsonia inermis L TERHADAP Trichophyton mentagrophytes

EKOLOGIA ◽

10.33751/ekol.v19i1.1657 ◽

2020 ◽

Vol 19 (1) ◽

pp. 12-19

Author(s):

Oom Komala ◽

Yulianita . ◽

Fuji Raka Siwi

Keyword(s):

Antifungal Activity ◽

Ethanol Extract ◽

Trichophyton Mentagrophytes ◽

Inhibition Zone ◽

Diffusion Method ◽

Dilution Method ◽

Disc Diffusion ◽

Lawsonia Inermis ◽

Disc Diffusion Method ◽

Mic Value

Trichopyton mentagrophytes is a type of fungus that belongs to a group of dermatophytes, and a disease that causes dermatophytosis (ringworm), this fungus likes parts that contain keratin substances such as skin, hair / hair, nails or horns. This study aims to determine the antifungal activity of 50% and 96% ethanol extract. KHM testing was made with a concentration of 2.5%, 5%, 7.5% and 10% with the dilution method to determine KHM. Whereas in testing the width of the inhibitory area (LDH) was carried out after getting the MIC value at concentrations of 10%, 25% and 50% with disc diffusion method to determine the effective LDH and qualitative phytochemical testing. The results showed that 96% ethanol solvent at a concentration of 50% could inhibit the fungus Trichopyton mentagrophytes compared to 50% ethanol as seen from the inhibition zone formed in the width of the inhibitory area. Secondary metabolite compounds found in 50% ethanol extract and 96% ethanol are flavonoids, alkaloids, saponins, tannins, quinones and terpenoids.

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Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220355 ◽

2021 ◽

Vol 22 (3) ◽

Author(s):

Muhammad Evy Prastiyanto ◽

NI’MATUR ROHMAH ◽

LESITA EFENDI ◽

RAHMATIA ARIFIN ◽

FANDHI ADI WARDOYO ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Trichophyton Rubrum ◽

Fungal Infections ◽

Curcuma Longa ◽

Ethanol Extract ◽

Pathogenic Fungi ◽

Zingiber Officinale ◽

Diffusion Method ◽

Antifungal Activities

Abstract. Prastiyanto ME, Rohmah N, Efendi L, Arifin R, Wardoyo FA, Wilson W, Mukaromah AH, Dewi SS, Darmawati S. 2021. Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum. Biodiversitas 22: 1509-1513. Fungal infections have now become serious health issues. One of the strategies to avoid the problems of fungal infections is by using natural product from plants that are effective against many human pathogenic fungi. The study portrayed the use of the extracts of plant rhizomes as the alternatives to fight against number of human pathogenic fungi. This research aimed to investigate the antifungal activities of crude ethanol extract of five member of the family Zingiberaceae (Curcuma longa, Alpinia galanga Zingiber officinale. var. rubrum, Zingiber officinale var. officinarum and Zingiber officinale var. amarum), which are widely used as folk medicines against Candida albicans and Trichophyton rubrum. Crude ethanol extracts of five members of Zingiberaceae were evaluated for their antifungal activities and the results were calculated based on the zones of inhibition using the diffusion method. The extract showed antifungal activity against Candida. albicans in the agar well diffusion assay (10.2-27.1 mm inhibition diameter) and against T. rubrum (27.3-44.3 mm inhibition diameter). The data have revealed that all rhizomes have the potential to be developed as antifungal agents, particularly against C. albicans and T. rubrum. Studies on the antifungal activity against yeast-like (C. albicans) and filamentous (T. rubrum) can provide new information about the benefits of members Zingiberaceae as a source of natural antifungal. Researchers can select the type of rhizome that has more potential for further extraction to obtain pure compounds that can be used as antifungals.

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