scholarly journals Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans

WARTA FARMASI ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 71-80
Author(s):  
Ummu Kalsum ◽  
Ayu Ayu
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Daucus Carota ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Daucus Carota L ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Activity Test ◽  
Agar Diffusion Method

ABSTRAK Telah dilakukan penelitian Uji Aktivitas Ekstrak Etanol Umbi Wortel (Daucus carota L.) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol umbi wortel sebagai antifungi terhadap pertumbuhan Candida albicans dengan menggunakan variasi konsentrasi yang berbeda yaitu 1% b/v, 3% b/v dan 5% b/v. Ekstrak etanol umbi wortel diperoleh dengan cara maserasi menggunakan pelarut etanol 70%. Pengujian aktivitas antifungi menggunakan metode difusi agar dengan cara sumuran untuk mengetahui aktivitas antifungi dengan mengamati daerah hambatan. Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak 1% b/v, 3% b/v dan 5% b/v dapat memberikan aktivitas yang menghambat pertumbuhan jamur uji. Terdapat penambahan diameter zona hambat pada setiap kenaikan konsentrasi yaitu 1% b/v (15,7 mm), 3% b/v (18,9 mm), dan 5% b/v (19,8 mm) terhadap jamur Candida albicans. Semua variasi konsentrasi ekstrak memiliki aktivitas antifungi yang kuat dalam menghambat pertumbuhan Candida albicans. Kata kunci: Ekstrak, Daucus carota L., Antifungi, Candida albicans ABSTRACT It has conducted a study of activity test of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans. This the study aims at knowing the activity of carrot tuber ethanol extract (Daucus carota L.) as antifungal toward the growth of candida albicans by using different variance concentration they are 1% b/v, 3% b/v and 5% b/v. Carrot tuber ethanol the extract was obtained by maceration by using 70% of ethanol dissolver. Activity test of antifungal by using agar diffusion method with a well method to find out the the activity of antifungal by observing the obstruction zone. The result of this research shows that concentration extract 1% b/v, 3% b/v and 5% b/v can give activity which impedes the growth of fungal test. There is increasing of obstruction zone diameter in every increment of of the concentration they are 1% b/v (15,7 mm), 3% b/v (18,9 mm) and 5% b/v (19,8mm) toward Candida albicans fungi. All extract concentration variation has a strong antifungal activity in impeding the growth of Candida albicans.

scholarly journals Uji aktivitas antifungi ekstrak etanol daun cabai jawa (Piper retrofractum) terhadap pertumbuhan candida albicans

2013 ◽  
Vol 11 (2) ◽  
pp. 36-42
Author(s):  
EVI ROSYIDA SARI ◽  
ESTU RETNANINGTYAS NUGRAHENI
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Activity Test ◽  
Significant Difference ◽  
Piper Retrofractum ◽  
One Way Anova ◽  
Concentration Series ◽  
Selection Of

Sari ER, Nugraheni ER. 2013. Antifungal activity test of Piper retrofractum leaf ethanol extract on Candida albicans growth. Biofarmasi 13: 36-42. Candida albicans is a pathogenic microbe infecting vagina, thrush (fungal infection on mouth cavity) and paronichia (the presence of pus on nail pad). The less effective treatment system, and the incidence of toxicity on several antifungal lead to the selection of alternative medication (treatment) from the secondary metabolite compounds of flavonoid, alkaloid and saponin existing in java chili (Piper retrofractum Vahl) leaf. This research aimed to examine the antifungal activity of javanese chili (Piper retrofractum Vahl) leaf ethanol extract on Candida albicans, as well as to determine the antifungal properties of antifungal activity of javanese chili leaf ethanol extract. The extract was obtained by maceration using 70% ethanol. The ethanol extract obtained was 57.895 gram with the specimen of 7.61% (b/w). The result of phytochemical screening showed the presence of flavonoid, saponin and alkaloid compounds, all of those were antifungal. The examination of antifungal activity of javanese chili leaf ethanol extract was performed by using a diffusion method and the concentration series of 10-100% by adding DMSO as a diluent and it was performed with 3 times of repetition. The result of DDH was analyzed using CRD (Completely Random Design) with one-way ANOVA at a confidence interval of 95% and LSD to find out the significant difference between the concentration series. The result of antifungal activity examination showed that the ethanol extract at 40% concentration provided more effective DDH on C. albicans of 5.54±0,64 mm. The result of examination was processed by using one-way ANOVA and LSD indicating a significant difference (p<0.05) between the concentration series. At 40% concentration, the antifungal examination was performed on C. albicans. The result showed that the javanese chili leaf ethanol extract was a fungiostatic against the tested fungus.

scholarly journals ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

2020 ◽  
Vol 3 (2) ◽  
pp. 76-81
Author(s):  
Rini Lestari
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Average Concentration ◽  
Diffusion Method ◽  
Microsporum Canis ◽  
Malassezia Furfur ◽  
Agar Diffusion Method ◽  
Alpinia Purpurata ◽  
Anti Fungal

Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI SPONS Phyllospongia lamellosa DARI PERAIRAN TUMBAK, MINAHASA TENGGARA TERHADAP PERTUMBUHAN MIKROBA Escherichia coli, Staphylococcus aureus DAN Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 815
Author(s):  
Kartini Ratu ◽  
Herny E. I. Simbala ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Average Value ◽  
Activity Test ◽  
Paper Disk ◽  
Agar Diffusion Method

ABSTRACT Sponges are a component of coral reef biota. These sea animals are known to contain compounds that have the potential to be developed in the field of medicine, including as an antimicrobial. This study aims to determine the antimicrobial activity of Phyllospongia lamellosa sponge against the growth of Staphylococcus aureus, Escherichia coli and Candida albicans collected in the waters of Tumbak, Posumaen District, Southeast Minahasa. The antimicrobial activity test was carried out by agar diffusion method. The results showed that the extract and fraction of Phyllospongia lamellose had antimicrobial activity seen in the inhibition zone formed around the paper disk against the test microbes. Ethanol extrack and fraction from Phyllospongia lamellosa sponge showed the greatest antimicrobial activity against Candida albicans with an average value of 13,33 mm was categorized as strong , than in Staphylococcus aureus with an average value of 13 mm is categorized as strong and on Escherichia coli 11 mm categorized as strong. Keywords  :Phyllospongia lamellosa, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang.  Hewan laut ini diketahui mengandung senyawa- senyawa yang berpotensi untuk dikembangkan dalam bidang pengobatan, diantaranya sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba spons Phyllospongia Lamellosa terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Candida albicans yang diambil pada perairan Tumbak Kecamatan Posumaen, Minahasa Tenggara. Uji aktifitas antimikroba dilakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak dan fraksi spons Phyllospongia lamellose memiliki aktifitas antimikroba dilihat zona hambat yang terbentuk disekitar cakram kertas terhadap mikroba uji. Ekstrak etanol dan fraksi dari Spons Phyllospongia lamellosa menunjukkan aktivitas antimikroba paling kuat terhadap candida albicans dengan nilai rata-rata 13,33 mm dikategorikan kuat, kemudian pada Staphylococcus aureus dengan nilai rata-rata 13 mm dikategorikan kuat, dan pada Escherichia coli 11 mm dikategorikan kuat. Kata Kunci :  Phyllospongia lamellosa, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

scholarly journals Perbandingan Aktivitas Antifungi Ekstrak Etanol 70% Dan 96% Buah Parijoto (Medinilla speciosa) Terhadap Candida albicans

2021 ◽  
Vol 3 (1) ◽  
pp. 32-41
Author(s):  
Puji Astutik ◽  
Richa Yuswantina ◽  
Rissa Laila Vifta
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
T Test ◽  
Diffusion Method ◽  
P Value ◽  
Fruit Extract ◽  
Fungal Activity ◽  
Activity Test ◽  
Ethanol Ethanol ◽  
Anti Fungal

Parijoto fruit (Medinilla speciosa) contains active compounds of flavonoids, tannins, saponins, glycosides. Parijoto fruit (Medinilla speciosa) has many benefits for human health, one of which is as an anti-fungal. In this study, the antifungal activity test of 70% ethanol extract and 96% ethanol extract of parijoto fruit (Medinilla speciosa) was tested against Candida albicans. This research was conducted by maceration using 70% ethanol and 96% ethanol as solvents. While the anti-fungal activity used the disc diffusion method using a concentration variation of 2.5% w / v; 5% w / v and 10% w / v using the ratio of ketoconazole antibiotics. Parijoto fruit extract (Medinilla speciosa) obtained 70% (%) ethanol and 96% (%) ethanol. Ethanol 70% with a concentration of 2.5% w / v of 25.83 mm; 5% w / v was 27.03 mm and 10% w / v was 28.03 mm, while the ethanol extract of 96% parijoto fruit (Medinilla speciosa) at a concentration of 2.5% w / v was 31.59 mm; 5% w / v of 33.24 mm and 10% w / v of 36.11 mm. The statistical results of 70% ethanol and 96% ethanol, both of which have anti-fungal activity, are not much different, as evidenced by the T-Test statistical test with a P-Value of 0.00 <0.05, because the effect of the 96% parijoto fruit compound is more effective. . The 70% ethanol extract and 96% ethanol extract of parijoto fruit (Medinilla speciosa) can inhibit the growth of Candda albicans with a concentration of 10%. ABSTRAK Buah Parijoto (Medinilla speciosa) mengandung senyawa aktif flavonoid, tanin, saponin, glikosida. Buah parijoto (Medinilla speciosa) merupakan yang memiliki banyak manfaat bagi kesehatan manusia, salah satunya adalah sebagai antifungi. Dalam penelitian ini, uji aktivitas antifungi dari ekstrak etanol 70% dan ekstrak etanol 96% buah parijoto (Medinilla speciosa) akan diuji terhadap Candida albicans. Penelitian ini dilakukan dengan maserasi menggunakan pelarut etanol 70% dan etanol 96%. Sedangkan aktivitas antifungi menggunakan metode difusi cakram menggunakan variasi kosentrasi 2,5% b/v; 5% b/v dan 10% b/v dengan menggunakan perbandingan antibiotik ketokonazole. Ekstrak buah parijoto (Medinilla speciosa) diperoleh hasil etanol 70% (%) dan etanol 96% (%). Etanol 70% dengan konsentrasi 2,5% b/v sebesar 25,83 mm; 5% b/v sebesar 27,03 mm dan 10% b/v sebesar 28,03 mm sedangkan ekstrak etanol 96% buah parijoto (Medinilla speciosa) pada konsentrasi 2,5% b/v sebesar 31,59 mm; 5% b/v sebesar 33,24 mm dan 10% b/v sebesar 36,11 mm. Hasil statistik etanol 70% dan etanol 96% aktivitas antifungi keduanya memiliki aktivitas antifungi yang tidak jauh berbeda sebagaimana dibuktikan dari uji statistik T-Test dengan nilai P-Value 0,00 <0,05, karena pengaruh dari senyawa buah parijoto 96% lebih efektif. Ekstrak etanol 70% dan etanol 96% buah parijoto (Medinilla speciosa) dapat menghambat pertumbuhan Candda albicans dengan kosentrasi 10%.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF WHITE AND RED FLESH FROM GUAVA LEAF ( Psidium guajava. L) AGAINTS Staphylococcus aureus AND Escherichia coli

2016 ◽  
Vol 16 (1) ◽  
pp. 51-56
Author(s):  
Hilda Maysarah ◽  
Rika Apriani ◽  
Misrahanum Misrahanum
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Guava Leaf ◽  
Mic Value

An antibacterial activity test of ethanol extract of white and red flesh from guava leaf (Psidium guajava. L) against S.aureus and E.coli; using agar diffusion method was carried out in order to produce the extract. The extract was collected using maceration method. The concentration of extract was 7,8125; 6,1035; 5,00; 4,8828; 4,3944; and 3,90625 mg/mL. The results showed that both of extracts had antibacterial activities. Ethanol extract of white flesh of fruit guava leaf had (Minimum Inhibitory Concentration) MIC value at 5.000 mg/mL against S.aureus and 4.8828 mg/mL against E.coli. Whereas ethanol extract of red flesh of fruit guava leaf had MIC value at 4.3944 mg/mL against S.aureus and E.coli.  MIC value of ethanol extract of white flesh of fruit guava leaf is equal with MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli. The MIC value of red flesh of fruit guava leaf is equal to the MIC value of clindamicin concentration at 3.00 µg/mL against S.aureus, and 1.00 µg/mL against E.coli.

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI ALGA (Halimeda opuntia) TERHADAP Escherichia coli, Staphylococcus aureus dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 178
Author(s):  
Shinta Jolanda ◽  
Defny S Wewengkang ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Green Algae ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

ABSTRACT Algae Halimeda opuntia is one of the green algae type that contains bioactive compounds which can be used for the treatment of various diseases. This study aimed to determine the antimicrobial activity of extracts and fractions of algae Halimeda opuntia obtained from the Lembeh Bitung Strait against Escherichia coli, Staphylococcus aureus and Candida albicans. The samples were extracted by maceration using ethanol solvent and fractionation using methanol, chloroform, and n-hexane solvents. Antimicrobial activity was carried out by the agar diffusion method of paper discs. The results showed that ethanol extract was able to inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria in the medium inhibitory category. While the methanol and chloroform fractions were only able to inhibit the growth of Staphylococcus aureus bacteria. The extract and all fractions did not show activity inhibiting the growth of Candida albicans fungi. Keywords: Algae, Halimeda opuntia, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans ABSTRAKAlga Halimeda opuntia merupakan salah satu jenis alga hijau yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari ekstrak dan fraksi alga Halimeda opuntia yang diperoleh dari Selat Lembeh Bitung terhadap Escherichia coli, Staphylococcus aureus dan Candida albicans. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antimikroba dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol, mampu menghambat pertumbuhan bakteri  Staphylococcus aureus dan Escherichia coli dengan kategori daya hambat sedang. Sedangkan  fraksi metanol dan fraksi kloroform hanya mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Ekstrak dan semua fraksi tidak menunjukkan aktivitas menghambat pertumbuhan jamur Candida albicans. Kata kunci: Alga, Halimeda opuntia, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

scholarly journals Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Muhammad Evy Prastiyanto ◽  
NI’MATUR ROHMAH ◽  
LESITA EFENDI ◽  
RAHMATIA ARIFIN ◽  
FANDHI ADI WARDOYO ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Fungal Infections ◽  
Curcuma Longa ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Antifungal Activities

Abstract. Prastiyanto ME, Rohmah N, Efendi L, Arifin R, Wardoyo FA, Wilson W, Mukaromah AH, Dewi SS, Darmawati S. 2021. Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida albicans and Trichophyton rubrum. Biodiversitas 22: 1509-1513. Fungal infections have now become serious health issues. One of the strategies to avoid the problems of fungal infections is by using natural product from plants that are effective against many human pathogenic fungi. The study portrayed the use of the extracts of plant rhizomes as the alternatives to fight against number of human pathogenic fungi. This research aimed to investigate the antifungal activities of crude ethanol extract of five member of the family Zingiberaceae (Curcuma longa, Alpinia galanga Zingiber officinale. var. rubrum, Zingiber officinale var. officinarum and Zingiber officinale var. amarum), which are widely used as folk medicines against Candida albicans and Trichophyton rubrum. Crude ethanol extracts of five members of Zingiberaceae were evaluated for their antifungal activities and the results were calculated based on the zones of inhibition using the diffusion method. The extract showed antifungal activity against Candida. albicans in the agar well diffusion assay (10.2-27.1 mm inhibition diameter) and against T. rubrum (27.3-44.3 mm inhibition diameter). The data have revealed that all rhizomes have the potential to be developed as antifungal agents, particularly against C. albicans and T. rubrum. Studies on the antifungal activity against yeast-like (C. albicans) and filamentous (T. rubrum) can provide new information about the benefits of members Zingiberaceae as a source of natural antifungal. Researchers can select the type of rhizome that has more potential for further extraction to obtain pure compounds that can be used as antifungals.

scholarly journals Comparative Analysis on the Antimycotic Effect of Azadiracta indica and Jatropha curcas on Curvularia Species

2021 ◽  
pp. 50-57
Author(s):  
E. E. Ameh ◽  
A. A. Orukotan ◽  
A. J. Dadah ◽  
A. A. Abdullahi
Keyword(s):  
Antifungal Activity ◽  
Aqueous Extract ◽  
Jatropha Curcas ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Isolation And Identification ◽  
Extract Concentration ◽  
Significant Difference ◽  
Curvularia Species

This study was carried out to determine the phytochemical properties and the antimycotic effect of Jatropha curcas and Azadiracta indica leaves on Curvularia species. Isolation and identification of the fungal isolates were carried out using standard conventional method. Maceration technique was employed for crude extraction from the plant. The phytochemical tests were carried out using standard conventional methods. Agar well diffusion method was used to determine the susceptibility of the isolates.  Phytochemical tests of A. indica revealed the presence of flavonoids, phenols, saponins, tannins, phenols, and glycosides.  Jatropha curcas showed the presence of glycoside, tannins, saponin steroids and phenols. The antifungal activity of Azadiracta indica and Jatropha curcas on Curvularia species, the ethanol extract of Azadiracta indica showed more antifungal activity with highest percentage inhibition of 50.56±0.84c at 20% extract concentration, and Aqueous extract was 40.00±0.55b at 20%. The aqueous extract of Jatropha curcas showed more antifungal activity of 50.55±0.05c at 20% while that of ethanol extract was 35.55±0.54a at 20% extract concentration. The synergestic antifungal activity of the plants revealed that ethanolic extract had more antifungal effect of 57.54±0.56c at 20% concentration and that of the aqeous extract showed more activity of 45.89±0.50b at 20%. There was significant difference (P<0.05) between the ethanoic and aqueous extracts of the plants. The Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of Jatropha curcas on Curvularia Species showed the MIC at 250 mg/ml extract concentration for both ethanol and aqueous extracts against Curvularia Species. The ethanol and aqueous extract of Jatropha curcas occurred has no fungicidal effect against Curvularia Species.

scholarly journals A comparative evaluation of Kumaun Himalayan Gymnosperms for their Antifungal potential against plant pathogenic fungi

2018 ◽  
Vol 7 (3) ◽  
pp. 230-241
Author(s):  
Savita Joshi ◽  
◽  
Parikshit Kumar ◽  
Prabha Pant ◽  
SC Sati ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Total Activity ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Cedrus Deodara ◽  
Araucaria Cunninghamii

Fungicidal activity of 10 ethnobotanically known Kumaun Himalayan gymnospermous plants namely Araucaria cunninghamii, Biota orientalis, Cedrus deodara, Cephalotaxus griffithi, Cryptomeria japonica Cupressus torulosa, Ginkgo biloba, Juniperus communis, Picea smithiana and Pinus wallichiana were tested against six plant disease causing fungal pathogens by agar well-diffusion method. Forty extracts of these gymnospermic leaves in different organic solvents (methanol, ethanol, chloroform and hexane) were studied by performing the 160 sets of experiments. The MIC values of each extract (where % inhibition ≥ 40%) were also determined. All the plant extracts exhibited strong antifungal activity. Results indicated that all leaves extracts of C. griffithi and G. biloba were found most effective among the tested plants extracts. Hexane extract of C. griffithi was showed highest inhibitory activity against C. falcatum (72%; MIC, 7.81µg/ml) and T. indica (70%; MIC, 15.62µg/ml). On the other hand, ethanol extract of G. biloba also showed remarkable activity against P. oryzae (66% with MIC, 7.81g/ml). While P. wallichiana leave extracts were found less active among the studied plants against all the tested fungal strains. The chloroform extracts were found the most effective against all the tested fungi (10% to 60%), followed by ethanol extract (30-50%), methanol extract (20-40%), while in hexane extracts ranged 10-30% only. The extracts of C. griffithi exhibited superior Relative Antifungal Activity (RAA, 20%), followed by G. biloba and A. cunninghamii (RAA, 19 and 12%, respectively). All data were also analyzed for determination of total activity of plant for each studied species of gymnosperm. C. griffithi had maximum activity i.e. 71 % followed by G. biloba (54%) and A. cunninghamii (33%). C. torulosa showed the least total activity and RAA i.e. 8% and 3%, respectively. All the plant species assayed possess definite antifungal properties and suggested for phytochemical analysis to identify the active principles responsible for their antifungal activity

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF SILVER NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE AND CANDIDA ALBICANS

2020 ◽  
Vol 8 (6) ◽  
pp. 179-187
Author(s):  
Titik Taufikurohmah ◽  
Tasha Anandya Tantyani
Keyword(s):  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Neisseria Gonorrhoeae ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Disk Diffusion Method ◽  
Fungal Inhibition ◽  
Inhibition Response ◽  
Antibacterial And Antifungal

This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.

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