Antibiofilm potential of Lactobacillus plantarum spp. cell free supernatant (CFS) against multidrug resistant bacterial pathogens

ABSTRACT The pleuromutilin antibiotic lefamulin demonstrated in vitro activity against the most relevant bacterial pathogens causing sexually transmitted infections (STI), including Chlamydia trachomatis (MIC 50/90 , 0.02/0.04 mg/liter; n = 15), susceptible and multidrug-resistant Mycoplasma genitalium (MIC range, 0.002 to 0.063 mg/liter; n = 6), and susceptible and resistant Neisseria gonorrhoeae (MIC 50/90 , 0.12/0.5 mg/liter; n = 25). The results suggest that lefamulin could be a promising first-line antibiotic for the treatment of STI, particularly in populations with high rates of resistance to standard-of-care antibiotics.

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Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants

Pharmaceuticals ◽

10.3390/ph13100266 ◽

2020 ◽

Vol 13 (10) ◽

pp. 266

Author(s):

Sam Woong Kim ◽

Song I. Kang ◽

Da Hye Shin ◽

Se Yun Oh ◽

Chae Won Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Influenza A ◽

Antibacterial Activities ◽

Proteinase K ◽

Bacterial Membranes ◽

Cell Free Supernatant ◽

Antiviral Activities ◽

Viral Envelopes ◽

Proteinase K Treatment ◽

Cellular Lysis

The recent pandemic of coronavirus disease 2019 (COVID-19) has increased demand for chemical disinfectants, which can be potentially hazardous to users. Here, we suggest that the cell-free supernatant from Lactobacillus plantarum NIBR97, including novel bacteriocins, has potential as a natural alternative to chemical disinfectants. It exhibits significant antibacterial activities against a broad range of pathogens, and was observed by scanning electron microscopy (SEM) to cause cellular lysis through pore formation in bacterial membranes, implying that its antibacterial activity may be mediated by peptides or proteins and supported by proteinase K treatment. It also showed significant antiviral activities against HIV-based lentivirus and influenza A/H3N2, causing lentiviral lysis through envelope collapse. Furthermore, whole-genome sequencing revealed that NIBR97 has diverse antimicrobial peptides, and among them are five novel bacteriocins, designated as plantaricin 1 to 5. Plantaricin 3 and 5 in particular showed both antibacterial and antiviral activities. SEM revealed that plantaricin 3 causes direct damage to both bacterial membranes and viral envelopes, while plantaricin 5 damaged only bacterial membranes, implying different antiviral mechanisms. Our data suggest that the cell-free supernatant from L. plantarum NIBR97, including novel bacteriocins, is potentially useful as a natural alternative to chemical disinfectants.

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Alkoxyphenylthiazoles with broad-spectrum activity against multidrug-resistant gram-positive bacterial pathogens

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2018.04.049 ◽

2018 ◽

Vol 152 ◽

pp. 318-328 ◽

Cited By ~ 14

Author(s):

Moustafa ElAwamy ◽

Haroon Mohammad ◽

Abdelrahman Hussien ◽

Nader S. Abutaleb ◽

Mohamed Hagras ◽

...

Keyword(s):

Broad Spectrum ◽

Bacterial Pathogens ◽

Multidrug Resistant ◽

Gram Positive ◽

Spectrum Activity ◽

Broad Spectrum Activity

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Potential bactericidal activity of S. nux-vomica–ZnO nanocomposite against multidrug-resistant bacterial pathogens and wound-healing properties

Journal of Trace Elements in Medicine and Biology ◽

10.1016/j.jtemb.2018.07.009 ◽

2018 ◽

Vol 50 ◽

pp. 229-239 ◽

Cited By ~ 5

Author(s):

Katherin Steffy ◽

G. Shanthi ◽

Anson. S. Maroky ◽

S. Selvakumar

Keyword(s):

Wound Healing ◽

Bactericidal Activity ◽

Bacterial Pathogens ◽

Multidrug Resistant

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Burden of multidrug resistant respiratory pathogens in intensive care units of tertiary care hospital

Asian Journal of Medical Sciences ◽

10.3126/ajms.v10i2.21098 ◽

2019 ◽

Vol 10 (2) ◽

pp. 14-19 ◽

Cited By ~ 1

Author(s):

Dharm Raj Bhatta ◽

Deependra Hamal ◽

Rajani Shrestha ◽

Supram HS ◽

Pushpanjali Joshi ◽

...

Keyword(s):

Intensive Care ◽

Respiratory Tract ◽

Intensive Care Units ◽

Lower Respiratory Tract ◽

Respiratory Tract Infections ◽

Bacterial Pathogens ◽

Multidrug Resistant ◽

Lower Respiratory Tract Infections ◽

Acinetobacter Species ◽

Tract Infections

Background: Lower respiratory tract infections are one of the most common infections among the patients in Intensive Care Units (ICUs). Admission in ICUs and use of life supporting devices increase the risk of infection with multidrug resistant pathogens. Aims and Objectives: This study was aimed to determine the prevalence and antibiograms ofthe bacterial pathogens causing lower respiratory tract infectionsamong patients of ICUs. Materials and Methods: A total of 184 specimens from patients admitted in ICUswith lower respiratory tract infections were included in this study. Isolation, identification and antibiotic susceptibility testing of the isolates was performed by standard microbiological techniques. Carbapenamase detection was performed by modified Hodge test method.Detection of metallo beta lactamase (MBL) was tested by imipenem and imipenem/EDTA disc. Detection of Klebsiellapneumoniaecarbapenamase (KPC) was performed by imipenem and imipenem/phenyl boronic acid. Results: Out of 184 samples, 131 showed significant growth of bacterial pathogens. Acinetobacter species (42.6%), Staphylococcus aureus (16.9%) and Pseudomonasaeruginosa(13.9%)were the three most common isolates. Out of 22 imipenem resistant isolates of Acientobacter species, 9 were KPC producer, 4 were MBL producers and 3 isolates were positive for MBL and KPC both. Among the Acinetobacter species, 5.1% isolates were resistant to tigecycline and colistin. One isolate of Pseudomonas aeruginosa was positive for MBL. Conclusions:High prevalence of multidrug resistant bacteria in ICUs was recorded. Gram negative bacilli were predominantly associated with LRTI among ICU patients;Acinetobacterspecies being most common isolate. Detection of carbapenamase among the Acinetobacterand emergence of tigecycline resistancelimits the therapeutic options.Regular monitoring of such resistant isolates would be important for managing infection control in critical units.

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2665. Intestinal Microbiome of Patients Submitted to Hematopoietic Stem Cell Transplantation Using Lactobacillus plantarum to Decolonized Multidrug-Resistant Bacteria

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.2343 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S933-S934

Author(s):

Bruna G C Moraes ◽

Roberta C R Martins ◽

Lucas A M Franco ◽

Victor A C C Lima ◽

Gaspar C O Pereira ◽

...

Keyword(s):

Stem Cell ◽

Hematopoietic Stem Cell Transplantation ◽

Stem Cell Transplantation ◽

Lactobacillus Plantarum ◽

Cell Transplantation ◽

Multidrug Resistant ◽

Intestinal Microbiome ◽

Resistant Bacteria ◽

Multidrug Resistant Bacteria ◽

Hematopoietic Stem

Abstract Background Patients colonized by multidrug-resistant bacteria (MDR) have high risk for infection after hematopoietic stem cell transplantation (HSCT). Probiotic is a strategy that can be used to decolonize patients. Our aim was to describe the impact of use of Lactobacillus plantarum (LP) on decolonization, MDR infections and intestinal microbiome (IM) of autologous HSCT patients colonized by vancomycin-resistant Enterococcus (VRE) and carbapenem-resistant Gram-negative (CRGN). Methods A prospective study was conducted at Hospital das Clinicas of University of Sao Paulo Brazil from 2017 to 2018. Candidates for auto-HSCT colonized by MDR received LP in capsules, 5 × 109 CFU twice daily until neutropenia (NP). Rectal swabs were performed and cultured using selective media as well as PCR for carbapenemases, vanA and vanB. Stool samples for IM analysis were collected weekly as baseline (before LB use) until the NP. The V4 region of 16S rDNA gene were sequenced by Ion Torrent PGM and analyzed using alpha and β diversities by Qiime. Demographic and clinical data including previous antibiotics use were evaluated; CDC criteria was applied for colonization and infection. Results All of seven patients were colonized by VRE and CRGN (Table 1). Only one patient remained colonized by CRGN after 61 days of LP. Four patients developed seven bloodstream infections (BSI) during the NP, two of them by CRGN. There was no infection caused by VRE neither by LP. One patient, with low adherence to LP use (66%), died due to MDR K. pneumoniae BSI. We observed a decrease of Clostridia, Verrucomicrobiae, Blautia and an increase of Enterobacteriaceae. Baseline samples from patients who used TMP/SMX had higher concentrations of Bacteroidetes when compared with those who had not use it. The Shannon index in controls ranged 1.98–5.55 and during NP 1.15–5.99. The β diversity analysis showed no clear patterns between patients. Conclusion We observed a heterogeneity among IM of auto-HSCT patients prior and after LP. It was not possible to establish an IM pattern, probably, because of small number of patients. Although, clinical infections by CRGN occurred despite of LP use, no cases of colonization and infection by VRE were identified. Thus, it seems that LP is a good and safe strategy to decolonized HSCT. Disclosures All authors: No reported disclosures.

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The biosynthesis of C55 polyprenols by a cell-free preparation of Lactobacillus plantarum

Biochemical Journal ◽

10.1042/bj1270345 ◽

1972 ◽

Vol 127 (2) ◽

pp. 345-349 ◽

Cited By ~ 9

Author(s):

I. F. Durr ◽

M. Z. Habbal

Keyword(s):

Mass Spectrometry ◽

Lactobacillus Plantarum ◽

De Novo ◽

Cell Free Supernatant ◽

Isoprene Unit ◽

A Cell

A cell-free supernatant of lysates of Lactobacillus plantarum catalyses the synthesis of lipids from [2-14C]mevalonate. Of the added mevalonate, 7.5% is incorporated into lipids, which were fractionated into five components. About 4% of the radioactivity in these lipids co-chromatographs with compounds shown by mass spectrometry, n.m.r. and i.r. spectroscopy to be C55 polyprenols, and about 2% co-chromatographs with a hexamer. The rest of the radioactivity is in more complex fractions. Analysis by mass spectrometry, n.m.r. and i.r. spectroscopy shows that the major C55 polyprenol is undecaprenol, accompanied by an isomer containing one reduced isoprene unit. A Kuhn–Roth degradation of [14C]polyprenols indicates that the supernatant catalyses synthesis of these compounds de novo.

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