scholarly journals Aktivitas Antibakteri Ekstrak Metanol Daun Turi (Sesbania grandiflora L.) Terhadap Pertumbuhan Klebseilla pneumonia

2020 ◽  
Vol 20 (2) ◽  
pp. 219
Author(s):  
Huurun Iien ◽  
Lalu Zulkifli ◽  
Prapti Sedijani
Keyword(s):  
Secondary Metabolites ◽  
Klebsiella Pneumoniae ◽  
Bacterial Growth ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Clear Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Sesbania Grandiflora ◽  
Activity Test

Abstrak: Turi merupakan tanaman yang memiliki berbagai khasiat diantaranya sebagai anti bakteri. Turi mengandung golongan senyawa tanin, saponin dan terpenoid yang dapat digunakan sebagai bahan dasar obat-obatan modern. Penelitian ini bertujuan untuk menentukan aktivitas ekstrak daun turi terhadap pertumbuhan Klebseilla pneumoniae berdasarkan diameter zona hambat. Pengujian dilakukan dengan mengekstraksi daun Turi dengan metode maserasi menggunakan pelarut metanol. Uji daya hambat antibakteri dilakukan dengan metode difusi sumuran. Hasil penelitian menunjukkan bahwa ekstrak daun turi memiliki daya hambat terhadap bakteri Klebsiella pneumoniae dengan konsentrasi 10%, 25%, 40% dan 55% dengan diameter zona hambatnya ialah 7.2mm, 14.4mm, 17.9mm, dan 22.5mm. Kemampuan penghambatan pertumbuhan bakteri pada metode sumuran untuk konsentrasi 55%, 40% dan 25% dikategorikan sangat kuat karena memiliki diameter zona hambat rata-rata >12 mm dan konsentrasi 10% di kategorikan lemah karena memiliki diameter zona hambat rata-rata <8 berdasarkan  kategori Vasanthakumari. Uji aktivitas antibakteri dalam menghambat pertumbuhan Klebsiella pneumoniae mempunyai nilai tertinggi pada konsentrasi 55% dibandingkan konsentrasi lainnya.Kata kunci: Ekstrak Daun Turi, maserasi, antibakteri, Klebseilla pneumoniae, Metabolit SekunderAbstract: Turi is a plant that has various of benefits include as anti-bacterial. Turi contains some compounds include thanin, saponins and terpenoids which can be used for modern medicines. This study aims to determine the activity of turi leaf extract on the growth of Klebseilla pneumoniae based on the inhibition zone diameter. The test was carried out by extracting Turi leaves by maceration method using methanol as a solvent. The antibacterial inhibition test was carried out by the wells method. The results showed that turi leaf extract has inhibitory properties against Klebsiella pneumoniae with concentrations of 10%, 25%, 40% and 55% with clear zone diameters of 7.2mm, 14.4mm, 17.9mm, and 22. 5mm. The ability to inhibit bacterial growth in methods of 55%, 40% and 25% is categorized as sangat kuat because it has an average inhibition zone diameter> 12 mm and for a concentration of 10% is categorized lemah because the average inhibition zone diameter is <8 based on the Vasanthakumari theory. Antibacteria activity test in inhibiting the growth of Klebsiella pneumoniae has the highest value at a concentration of 55% compared to other concentrations.Keywords: Turi Leaf Extract, maceration, antimicrobial, Klebseilla pneumoniae, Secondary Metabolites

scholarly journals UJI DAYA HAMBAT EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia purpurata K.Schum) TERHADAP PERTUMBUHAN BAKTERI Klebsiella pneumoniae ISOLAT SPUTUM PADA PENDERITA PNEUMONIA RESISTEN ANTIBIOTIK SEFTRIAKSON

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Poetry Melinda Abubakar ◽  
Fatimawali Fatimawali ◽  
Paulina Yamlean
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Growth ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Alpinia Purpurata ◽  
Inhibition Ability

ABSTRACT Red galangal rhizome is a plant that has various properties including antifungal and antibacterial. Red galangal rhizomes contain a class of flavonoids, phenols and terpenoids, which can be, used as basic ingredients of modern medicines. Flavonoids and phenols are also known to inhibit microbes. Flavonoids can inhibit microbes that have been resistant to antibiotics. This study aims to determine the antibacterial activity of red galangal rhizome extract (Alpinia purpurata K.Schum) and test its inhibitory effect on the growth of Klebsiella pneumoniae bacteria in sputum isolates in patients with pneumonia resistant ceftriaxone antibiotics. Red galangal rhizome plant was extracted by maceration method using 96% ethanol. Antibacterial inhibitory tests were carried out using the disc and well method. The results showed that the red galangal extract of rhizome had a inhibitory effect on the bacterium Klebsiella pneumoniae. On the disc method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone is 9.6 mm, 8.68 mm, 8.5 mm, 8.5 mm and 7.6 mm. The inhibition ability of bacterial growth on the disc method is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Whereas in the well method for concentrations of 100%, 75%, 50%, 25% and 12.5% the diameter of the inhibition zone was 25.8 mm, 20 mm, 15.6 mm, 12.7 mm and 9.7 mm, respectively. The inhibition ability of bacterial growth in the well method for 100% concentration is categorized as very strong because it has an average inhibition zone diameter of >20 mm, for concentrations of 75%, 50% and 25% it is categorized as strong because it has an average inhibition zone of 10- 20 mm and for a concentration of 12.5% it is categorized as moderate because it has an average inhibition zone diameter of 5-10 mm. Keywords: Red galangal rhizome, Alpinia purpurata K.Schum, Klebsiella pneumonia  ABSTRAK Rimpang Lengkuas merah merupakan tanaman yang memiliki berbagai khasiat di antaranya sebagai antijamur dan antibakteri. Rimpang Lengkuas merah mengandung golongan senyawa flavonoid, fenol dan terpenoid yang dapat digunakan sebagai bahan dasar obat-obatan moderen. Senyawa flavonoid dan fenol juga diketahui dapat menghambat mikroba. Flavonoid dapat menghambat mikroba yang telah resisten terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak rimpang Lengkuas merah (Alpinia purpurata K.Schum) dan menguji daya hambatnya terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat sputum pada penderita pneumonia resisten antibiotik seftriakson. Tanaman rimpang Lengkuas merah di ekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Uji daya hambat antibakteri dilakukan dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak rimpang Lengkuas merah memiliki daya hambat terhadap bakteri Klebsiella pneumoniae. Pada metode cakram untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 9,6 mm, 8,68 mm, 8,5 mm, 8,5 mm dan 7,6 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode cakram di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 100%, 75%, 50%, 25% dan 12,5% diameter zona hambatnya ialah 25,8 mm, 20 mm, 15,6 mm, 12,7 mm dan 9,7 mm. Kemampuan penghambatan pertumbuhan bakteri pada metode sumuran untuk konsentrasi 100% di kategorikan sangat kuat karena memiliki diameter zona hambat rata-rata >20 mm, untuk konsentrasi 75%, 50% dan 25% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm dan untuk konsentrasi 12,5% di kategorikan sedang karena memiliki diameter zona hambat rata-rata 5-10 mm. Kata Kunci:Rimpang Lengkuas merah,  Alpinia purpurata K.Schum, Klebsiella pneumonia

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals POTENCY AND ACTIVITY OF SECONDARY METABOLITE OF Trichoderma harzianum AC1(b) J2 INHIBITOR GROWTH Colletotrichum capsici IPBCC 13.1098

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Nurkayah Nurkayah ◽  
Elisa Nurnawati ◽  
Hary Widjajanti
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Trichoderma Harzianum ◽  
Inhibitory Concentration ◽  
Pathogenic Fungus ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Colletotrichum Capsici ◽  
Layer Chromatography

Trichoderma harzianum is a fungus that can produce secondary metabolites which able to inhibit the growth of Colletotrichum capsici. a pathogenic fungus causing anthracnose in plants. the Aims of this research were to obtain and analyze the ability of secondary metabolites of Trichoderma harzianum AC1 (b) J2 isolated from Scleria poaeformis. to inhibit the growth of Colletotrichum capsici IPBCC13.1098. The secondary metabolite eluat of Trichoderma harzianum were tested for the antifungal activity toward Colletotrichum capsici IPBCC 13.1098. Fractionation was carried out by Column Chromatography and produced 31 eluat. The secondary metabolite eluat of Trichoderma harzianum with the highest inhibition zone diameter was eluat 1 with 8.4 mm in diameter. The secondary metabolite eluat with the highest value of inhibition zone was carried out by MIC test and thin layer chromatography (TLC). Based on the MIC results, the minimum inhibitory concentration of the secondary metabolite of Trichoderma harzianum to fungus Colletotrichum capsici was 250 ppm with inhibition zone diameter was 0.10 mm. The results of TLC showed orange spots on the TLC plate that indicated alkaloite compounds.

scholarly journals Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 101
Author(s):  
Esterlina Aldora Puluh ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Persea Americana ◽  
Inhibition Zone Diameter ◽  
Inhibitory Ability ◽  
Activity Test

Daun Alpukat memiliki kandungan antara lain saponin, alkaloid, flavonoid, polifenol, quersetin yang bersifat sebagai antibakteri.  Penelitian ini bertujuan untuk mendapatkan konsentrasi terbaik dari ekstrak etanol daun alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off berdasarkan uji aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan masker gel peel off ekstrak daun Alpukat dibuat dengan variasi konsentrasi 0.1%; 0.15%; 0.2%; 0.25% dan 0.3%. Ekstrak tanaman daun alpukat diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian antibakteri dengan metode sumuran diameter zona hambat yang paling besar yaitu pada konsentrasi 0,3% dengan diameter zona hambat 3 mm ± 0,32 dan kemampuan daya hambat dikategorikan lemah. Dapat disimpulkan bahwa konsentrasi terbaik dari ekstrak etanol daun Alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off yaitu pada konsentrasi 0.3% dan dikategorikan kemampuan daya hambat lemahAvocado leaves contains saponins, alkaloids, flavonoids, polyphenols, quercetin which are as antibacterial properties. This study aims to obtain the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) which is formulated as a gel peel off mask preparation based on antibacterial activity test against Staphylococcus epidermidis bacteria. This study uses a laboratory experimental method. Avocado leaf extract peel off mask gel formula is made with a concentration variation of 0.1%; 0.15%; 0.2%; 0.25% and 0.3%. Avocado leaf extract is obtained by maceration using 96% ethanol solvent. Antibacterial testing with the largest inhibition zone diameter wells method is at a concentration of 0.3% with a diameter of inhibition zone 3 mm ± 0.32 and the ability of inhibition is categorized as weak. It can be concluded that the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) Which is formulated as a gel peel off mask preparation is at a concentration of 0.3% and categorized as weak inhibitory ability

scholarly journals EFFECTIVENESS OF UTILIZING MIANA LEAVES (COLEUS SCUTTELARIOIDES (L) BENTH) ON THE GROWTH OF ESCHERICHIA BACTERIA (E. COLI) AS A CAUSE OF DIARRHEA AT DRINKING WATER REFILL CENTER

2021 ◽  
Vol 56 (5) ◽  
pp. 494-503
Author(s):  
Nani Yuniar ◽  
Yusuf Sabilu ◽  
Juminten ◽  
Suryani ◽  
Ruslan Madjid ◽  
...  
Keyword(s):  
Drinking Water ◽  
Experimental Research ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Effective Concentration ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Extract Concentration ◽  
Significant Difference

This research aims to determine the inhibition and effectiveness of using miana leaf extract against the growth of E. coli bacteria that causes diarrhea. This study uses experimental research with samples of miana leaves extracted and E. coli bacteria and then uses the Kruskal-Wallis test with Mann-Whitney analysis to test the hypothesis. The results showed differences in the inhibition between miana leaves extract concentrations of 15%, 30%, 50%, and 90% of the growth of E. coli bacteria with Asymp values. Sig = 0.017. The most effective concentration of miana leaf extract in influencing E. coli bacteria was an extract concentration of 90% with an inhibition zone diameter of 31.27 mm. There was a significant difference in the inhibition between miana leaf extract (Coleus scutellarioides (L) Benth) on the growth of E. coli bacteria at 12, 15 & 18 hours, 20 hours, and 24 hours, with Asymp values. Sig = 0.000. Results an increase in the inhibition zone, and the highest inhibition occurred during the 24 hours, namely at a very strong 90% extract concentration. This finding proves that the compounds contained in miana leaf extract can inhibit the growth of E. coli bacteria. This finding has implications for refill drinking water managers to utilize miana leaf extract to avoid E. coli bacteria as the main cause of diarrhea.

scholarly journals Evaluasi Aktivitas Antibakteri Sediaan Plester Ekstrak Daun Petai Cina (Leucaena Leucocephala) Terhadap Bakteri Staphylococcus AureusAttc 25923 Pk/5

2021 ◽  
Vol 1 ◽  
pp. 665-670
Author(s):  
Eka Sri Rahayu ◽  
W Wirasti ◽  
S Slamet ◽  
Dwi Bagus Pambudi
Keyword(s):  
Antibacterial Activity ◽  
Leucaena Leucocephala ◽  
Leaf Extract ◽  
Dosage Form ◽  
Inhibition Zone ◽  
Test Method ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Inhibitory Power

AbstractChinese petai plants contain alkaloids that have antibacterial abilities including Staphylococcus aureusATTC 25923 PK/5 bacteria. Plaster is a dosage form whose active ingredients need to be developed from natural ingredients, especially Chinese petai leaves. The purpose of this study was to optimize the plaster of Chinese petai leaf extract and antibacterial Staphylococcus aureusATTC 25923 PK/5and to determine the antibacterial activity of the extract plaster with various doses. The test method for antibacterial is the well method. The data obtained is the diameter of the inhibitory power of the extract in the plaster. Data analysis to determine antibacterial activity using spss. The results of the phytochemical test showed that the Chinese petai leaf extract contained active compounds, namely tannins, saponins, alkaloids, flavonoids and steroids. Chinese petai leaf extract was also able to inhibit Staphylococcus aureusATTC 25923 PK/5bacteria in the extract obtained an inhibition zone of 5.1 mm at a concentration of 25% and plaster obtained an inhibition zone of 3.46 mm, a concentration of 50% obtained an inhibitory zone diameter of 5.63 mm and at plaster obtained an inhibition zone diameter of 4.5 mm, a concentration of 75% obtained an extract inhibition zone diameter of 6.56 mm on plaster obtained an inhibition zone diameter of 5.53 mm, a concentration of 100% obtained an extract inhibition zone diameter of 7 mm and on plaster The diameter of the inhibition zone was 6.46 mm. This shows that the Chinese petai leaf extract has potential as an antibacterial against the growth of Staphylococcus aureusATTC 25923 PK/5bacteria.Keywords: Chinese Petai, Extract, Plaster, Antibacterial AbstrakTanaman petai cina mengandung alkaloida yang memiliki kemampuan sebagai antibakteri diantaranya bakteri Staphylococcus aureusATTC 25923 PK/5. Plester merupakan bentuk sediaan yang bahan aktifnya perlu dikembangkan dari bahan alam terutama daun petai cina. Tujuan penelitian ini melakukan optimasi plester ekstrak daun petai cina dan antibakteri Staphylococcus aureusATTC 25923 PK/5dan mengetahui aktivitas antibakteri plester ekstrak dengan berbagai dosis. Metode uji terhadap antibakteri adalah metode sumuran. Data yang didapatkan adalah diameter daya hambat ekstrak dalam plester. Analisis data untuk mengetahui aktivitas antibakteri menggunakan ANOVA. Hasil uji fitokimia menunjukkan bahwa ekstrak daun petai cina memiliki kandungan senyawa aktif, yakni tanin, saponin, alkaloid, flavonoid dan steroid. Ekstrak daun petai cina juga mampu menghambat bakteri Staphylococcus aureusATTC 25923 PK/5pada ekstrak didapatkan zona hambat sebesar 5,1 mm pada konsentrasi 25% dan plester didapatkan zona hambat sebesar 3,46 mm, konsentrasi 50%didapatkan diameter zona hambat ekstrak sebesar 5,63 mm dan pada plester didapatkan diameter zona hambat sebesar 4,5 mm, konsentrasi 75% didapatkan diameter zona hambat ekstrak sebesar 6,56 mm pada plester didapatkan diameter zona hambat sebesar 5,53 mm, konsentrasi 100% didapatkan diameter zona hambat ekstrak sebesar 7 mm dan pada plester didapatkan diameter zona hambat sebesar 6,46 mm. Hal ini menunjukkan bahwa ekstrak daun petai cina memiliki potensi sebagai antibakteri terhadap pertumbuhan bakteri Staphylococcus aureusATTC 25923 PK/5.Kata kunci:Petai Cina, Ekstrak, Plester, Antibakteri

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL RIMPANGLENGKUAS PUTIH (Alpinia galanga L. Willd) TERHADAPBAKTERI Klebsiella pneumoniae ISOLAT URIN PADA PENDERITA INFEKSI SALURAN KEMIH

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 975
Author(s):  
Arini Shintia ◽  
Fatimawali Fatimawali ◽  
Jainer P. Siampa
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Inhibition Zone Diameter ◽  
Alpinia Galanga ◽  
Inhibitory Ability ◽  
Activity Test ◽  
Ethanol Extracts ◽  
Tract Infections

ABSTRACTWhite galangal rhizome is a plant that has properties such as antifungal and antibacterial. White galangal rhizome contains of active compounds, namely flavonoid, phenol and terpenoid compounds which can inhibit microbes. This study aimed to determine the antibacterial activity of ethanol extracts of white galangal rhizome against Klebsiella pneumoniae urine isolate in patients with urinary tract infections. White galangal rhizome plants were extracted using 96% ethanol solvent. Antibacterial activity test was performed using the disc and wells method. The result showed that ethanol extracts of white galangal rhizome has antibacterial activity against the bacteria Klebsiella pneumoniae. In the disc method with concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone formed are 9,8 mm; 9 mm; 7,8 mm; 7,9 mm and 7,7 mm. Inhibition of the disc method is categorized as medium because the diameter of the zone formed is only around an average of 5-10 mm. Whereas in the wells method for concentrations of 80%, 60%, 40%, 20% and 10% the diameter of the inhibition zone are 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm and 2,6 mm. The inhibitory ability at concentrations of 80% and 60% is categorized as strong because it has an average of 10-20 mm, for concentrations of 40% and 20% are categorized as medium, because it has an average inhibition zone diameter of 5-10 mm, and for concentration of 10% is categorized as weak because it has a inhibition diameter <5 mm.Keywords: White Galangal Rhizome (Alpinia galanga L. Willd), Klebsiella pneumonia ABSTRAKRimpang lengkuas putih merupakan tanaman yang memiliki khasiat di antaranya sebagai antifungi dan antibakteri. Rimpang Lengkuas putih mengandung golongan senyawa aktif yaitu golongan senyawa flavonoid, fenol dan terpenoid yang dapat menghambat mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol rimpang Lengkuas putih terhadap bakteri Klebisella pneumoniae isolat urin pada penderita infeksi saluran kemih. Tanaman rimpang Lengkuas putih di ekstraksi dengan menggunakan pelarut etanol 96%. Uji aktivitas antibakteri dengan metode cakram dan sumuran. Hasil penelitian menunjukkan bahwa ekstrak etanol rimpang Lengkuas putih memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae. Pada metode cakram dengan konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambat yang terbentuk ialah 9,8 mm; 9 mm; 7,8 mm; 7,9 mm dan 7,7 mm. Penghambatan pada metode cakram dikategorikan sedang karena diameter zona yang terbentuk hanya berkisar pada rata-rata 5-10 mm. Sedangkan pada metode sumuran untuk konsentrasi 80%, 60%, 40%, 20% dan 10% diameter zona hambatnya ialah 11,3 mm; 10,3 mm; 9,3 mm; 6,3 mm dan 2,6 mm. Kemampuan penghambatan pada konsentrasi 80% dan 60% di kategorikan kuat karena memiliki diameter zona hambat rata-rata 10-20 mm, untuk konsentrasi 40% dan 20% di kategorikan sedang, karena memiliki diameter zona hambat rata-rata 5-10 mm, dan untuk konsentrasi 10% di kategorikan lemah karena memiliki diameter zona hambat <5 mm. Kata Kunci: Rimpang lengkuas putih (Alpinia galanga L. Willd), Klebsiella pneumoniae

scholarly journals Antibacterial Effect of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 ON Klebsiella pneumoniae Producting Extended-Spectrum β-Lactamase (ESBL)

2019 ◽  
Vol 1 (1) ◽  
pp. 16-27
Author(s):  
Yuani Setiawati ◽  
Wiwin Retnowati ◽  
Achmad Basori
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Main Concern ◽  
Peak Activity ◽  
Streptomyces Sp ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Extended Spectrum

Klebsiella pneumoniae as an opportunistic pathogen can cause nosocomial infection.  The main concern on this bacterium is directed on the extended-spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae. The therapy of ESBL-producing Klebsiella pneumoniae infections is very limited because of its multidrug resistance. It had been found new local isolates Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 in mangrove East Coast of Surabaya. These isolates have potential to produce antibiotics (Retnowati, 2008). This study was aimed to prove these isolates may inhibit the growth of ESBL-producing Klebsiella pneumonia. The test of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 using the modification Agar print method against clinical isolates of ESBL-producing Klebsiella pneumoniae. The diameter of inhibition zone (mm) formed shows activity of these isolates. The profiles of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 were different in terms of periode required to reach peak activity, duration of activity and inhibition zone diameter produced during 10 days of observation. Streptomyces sp-MWS3 reached the peak activity most rapidly on day 3 with the largest inhibition zone diameter of 9 mm in ESBL-producing Klebsiella pneumoniae. There were significant differences in inhibition zone diameter between Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 against ESBL-producing Klebsiella pneumoniae.

scholarly journals EFEKTIVITAS BERBAGAI KONSENTRASI EKSTRAK DAUN KAYU MANIS (Cinnamomum burmannii [Ness.] BI) TERHADAP DIAMETER ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Jurnal Biota ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 12-18
Author(s):  
Moh. Syaifuddin Qomar ◽  
Moch. Agus Krisno Budiyanto ◽  
Sukarsono Sukarsono ◽  
Sri Wahyuni ◽  
Husamah Husamah
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Skin Infections ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Cinnamomum Burmannii ◽  
Biology Laboratory

Staphylococcus epidermidis is one of the bacteria that can cause infectious diseases. In general Staphylococcus epidermidis can cause diseases of swelling (abscess) such as skin infections or acne. Cinnamon (Cinnamomum burmannii [Ness.] BI) leaf extract has a substance which gives antimicrobial effects that can be used as an inhibitor of the Staphylococcus epidermidis bacteria’s growth because it contains substances such as essential oils, flavonoids, saponins, tannins, and alkaloids. The aim of this research is to analyse the effect of the concentration of cinnamon leaf extract and to determine the concentration of extract which has the best influence to the inhibition zone diameter of Staphylococcus epidermidis bacteria. This research was conducted in Biology Laboratory of University of Muhammadiyah Malang, on 17-18 May 2017. The results showed that there was an effect of giving various concentrations of cinnamon leaf extract to the inhibition zone diameter of Staphylococcus epidermidis bacteria’s growth. The concentration of cinnamon leaf extract which has the best influence to the inhibitory zone diameter of Staphylococcus epidermidis bacteria is 100% with the mean of inhibitory zone diameter 15.16 mm.

scholarly journals Uji antibakteri kombinasi ekstrak daun Biduri (Calotropis gigantea L.) dan daun Bandotan (ageratum conyzoides L.)

2020 ◽  
Vol 1 (2) ◽  
pp. 165
Author(s):  
Munira Munira ◽  
Fina Rodisa ◽  
Muhammad Nasir
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Ageratum Conyzoides ◽  
Inhibition Zone Diameter ◽  
Calotropis Gigantea ◽  
Microbiological Test ◽  
Zone Diameter

Background: Biduri leaves (Calotropis gigantea (L.) W.T. Aiton and Bandotan leaves (Ageratum conyzoides L.) are wild growing plants that are believed to be used as an antibacterial.Objectives: This research was conducted to determine the effect of combination ethanolic extract of Biduri and Bandotan leaves against Staphylococcus aureus and Staphylococcus epidermidis.Methods: This research is an experimental by using Completely Randomized Design (CRD) with 4 treatments which is P0 (aquadest), P1 (Biduri leaf extract), P2 (Bandotan leaf extract), and P3 (combination of Biduri and Bandotan leaf extract) with 5 replications. Microbiological test used was the diffusion method.Results: The results of Anova showed that combination of Biduri and Bandotan leaf extract very influential in the growth of Staphylococcus aureus dan Staphylococcus epidermidis. Duncan further test results showed that the largest average inhibition zone diameter in inhibiting Staphylococcus aureus was formed by P2 which is 14.14 mm, significantly different from P1 (9.46 mm) and P3 (11.20 mm), but P1 is not significantly different from P3. Toward Staphylococcus epidermidis, the largest average inhibition zone diameter also formed by P2 which is 17.06 mm that is also significantly different from P1 (10.14 mm) and P3 (12.66 mm), and P1 is not significantly different from P3.Conclusion: The combination of Biduri and Bandotan leaf extract very influential against Staphylococcus aureus and Staphylococcus epidermidis.

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