scholarly journals Evaluasi Aktivitas Antibakteri Sediaan Plester Ekstrak Daun Petai Cina (Leucaena Leucocephala) Terhadap Bakteri Staphylococcus AureusAttc 25923 Pk/5

2021 ◽  
Vol 1 ◽  
pp. 665-670
Author(s):  
Eka Sri Rahayu ◽  
W Wirasti ◽  
S Slamet ◽  
Dwi Bagus Pambudi
Keyword(s):  
Antibacterial Activity ◽  
Leucaena Leucocephala ◽  
Leaf Extract ◽  
Dosage Form ◽  
Inhibition Zone ◽  
Test Method ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Inhibitory Power

AbstractChinese petai plants contain alkaloids that have antibacterial abilities including Staphylococcus aureusATTC 25923 PK/5 bacteria. Plaster is a dosage form whose active ingredients need to be developed from natural ingredients, especially Chinese petai leaves. The purpose of this study was to optimize the plaster of Chinese petai leaf extract and antibacterial Staphylococcus aureusATTC 25923 PK/5and to determine the antibacterial activity of the extract plaster with various doses. The test method for antibacterial is the well method. The data obtained is the diameter of the inhibitory power of the extract in the plaster. Data analysis to determine antibacterial activity using spss. The results of the phytochemical test showed that the Chinese petai leaf extract contained active compounds, namely tannins, saponins, alkaloids, flavonoids and steroids. Chinese petai leaf extract was also able to inhibit Staphylococcus aureusATTC 25923 PK/5bacteria in the extract obtained an inhibition zone of 5.1 mm at a concentration of 25% and plaster obtained an inhibition zone of 3.46 mm, a concentration of 50% obtained an inhibitory zone diameter of 5.63 mm and at plaster obtained an inhibition zone diameter of 4.5 mm, a concentration of 75% obtained an extract inhibition zone diameter of 6.56 mm on plaster obtained an inhibition zone diameter of 5.53 mm, a concentration of 100% obtained an extract inhibition zone diameter of 7 mm and on plaster The diameter of the inhibition zone was 6.46 mm. This shows that the Chinese petai leaf extract has potential as an antibacterial against the growth of Staphylococcus aureusATTC 25923 PK/5bacteria.Keywords: Chinese Petai, Extract, Plaster, Antibacterial AbstrakTanaman petai cina mengandung alkaloida yang memiliki kemampuan sebagai antibakteri diantaranya bakteri Staphylococcus aureusATTC 25923 PK/5. Plester merupakan bentuk sediaan yang bahan aktifnya perlu dikembangkan dari bahan alam terutama daun petai cina. Tujuan penelitian ini melakukan optimasi plester ekstrak daun petai cina dan antibakteri Staphylococcus aureusATTC 25923 PK/5dan mengetahui aktivitas antibakteri plester ekstrak dengan berbagai dosis. Metode uji terhadap antibakteri adalah metode sumuran. Data yang didapatkan adalah diameter daya hambat ekstrak dalam plester. Analisis data untuk mengetahui aktivitas antibakteri menggunakan ANOVA. Hasil uji fitokimia menunjukkan bahwa ekstrak daun petai cina memiliki kandungan senyawa aktif, yakni tanin, saponin, alkaloid, flavonoid dan steroid. Ekstrak daun petai cina juga mampu menghambat bakteri Staphylococcus aureusATTC 25923 PK/5pada ekstrak didapatkan zona hambat sebesar 5,1 mm pada konsentrasi 25% dan plester didapatkan zona hambat sebesar 3,46 mm, konsentrasi 50%didapatkan diameter zona hambat ekstrak sebesar 5,63 mm dan pada plester didapatkan diameter zona hambat sebesar 4,5 mm, konsentrasi 75% didapatkan diameter zona hambat ekstrak sebesar 6,56 mm pada plester didapatkan diameter zona hambat sebesar 5,53 mm, konsentrasi 100% didapatkan diameter zona hambat ekstrak sebesar 7 mm dan pada plester didapatkan diameter zona hambat sebesar 6,46 mm. Hal ini menunjukkan bahwa ekstrak daun petai cina memiliki potensi sebagai antibakteri terhadap pertumbuhan bakteri Staphylococcus aureusATTC 25923 PK/5.Kata kunci:Petai Cina, Ekstrak, Plester, Antibakteri

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

scholarly journals Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 112
Author(s):  
Theresia H. Tunas ◽  
Hosea Jaya Edy ◽  
Jainer Pasca Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Moringa Oleifera Leaves ◽  
Inhibitory Power ◽  
Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

scholarly journals EFEKTIVITAS BERBAGAI KONSENTRASI EKSTRAK DAUN KAYU MANIS (Cinnamomum burmannii [Ness.] BI) TERHADAP DIAMETER ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Jurnal Biota ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 12-18
Author(s):  
Moh. Syaifuddin Qomar ◽  
Moch. Agus Krisno Budiyanto ◽  
Sukarsono Sukarsono ◽  
Sri Wahyuni ◽  
Husamah Husamah
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Skin Infections ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Cinnamomum Burmannii ◽  
Biology Laboratory

Staphylococcus epidermidis is one of the bacteria that can cause infectious diseases. In general Staphylococcus epidermidis can cause diseases of swelling (abscess) such as skin infections or acne. Cinnamon (Cinnamomum burmannii [Ness.] BI) leaf extract has a substance which gives antimicrobial effects that can be used as an inhibitor of the Staphylococcus epidermidis bacteria’s growth because it contains substances such as essential oils, flavonoids, saponins, tannins, and alkaloids. The aim of this research is to analyse the effect of the concentration of cinnamon leaf extract and to determine the concentration of extract which has the best influence to the inhibition zone diameter of Staphylococcus epidermidis bacteria. This research was conducted in Biology Laboratory of University of Muhammadiyah Malang, on 17-18 May 2017. The results showed that there was an effect of giving various concentrations of cinnamon leaf extract to the inhibition zone diameter of Staphylococcus epidermidis bacteria’s growth. The concentration of cinnamon leaf extract which has the best influence to the inhibitory zone diameter of Staphylococcus epidermidis bacteria is 100% with the mean of inhibitory zone diameter 15.16 mm.

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI

2016 ◽  
Vol 9 (5) ◽  
pp. 69 ◽  
Author(s):  
Jansen Silalahi ◽  
Petrika Situmorang ◽  
Popi Patilaya ◽  
Yosy Ce Silalahi
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Coconut Oil ◽  
Inhibition Zone ◽  
Research Centre ◽  
Virgin Coconut Oil ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®

scholarly journals The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth

2014 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Galih Arif Kusuma ◽  
Sammy N. J. Longdong ◽  
Reiny A. Tumbol
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Bacteria Growth ◽  
Activity Test ◽  
Inhibitory Power

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.

Karakteristik Fisik dan Aktivitas Antibakteri Sabun Cair Minyak Nilam (Pogostemon cablin Benth.) yang Berbasis Surfaktan Sodium Lauril Eter Sulfat

2020 ◽  
pp. 1-10
Author(s):  
Yuni Anggraeni ◽  
Fakhrun Nisa' ◽  
Ofa Suzanti Betha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Foam Height ◽  
Inhibition Zone ◽  
Positive Control ◽  
Centrifuge Test ◽  
Liquid Soap ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pogostemon Cablin

Patchouli oil obtained from patchouli plants (Pogostemon cablin Benth.) has been widely used as an antibacterial agent in liquid and solid soap preparation. In this research, patchouli oil was added to liquid soap formula which use sodium lauryl ether sulphate (SLES) surfactant. The purpose of this study was to determine the physical characteristics and antibacterial activity of patchouli oil liquid soaps based on SLES surfactant. The Liquid soaps were prepared into four formulas with variation of patchouli oil concentration that were F0 (0.00% w/w), F1 (0.05% w/w), F2 (0.50% w/w), and F3 (1.00% w/w). The liquid soaps were evaluated including organoleptic, microscopic property, physical stability by using centrifuge test, pH, foam height and stability, viscosity and rheology, and antibacterial activity against Staphylococcus aureus ATCC 25923 by using disc diffusion method and chloramphenicol as a positive control. The results showed that all liquid soap formulas had following characteristics: translucent except F3 slightly murky, homogen, stable after centrifuge test, pH between 6.55 – 6.66 that were no significantly different and met SNI standards of liquid soap, foam height between 2.67 – 3.23 cm that were no significantly different and stable after 5 minutes of testing, viscosity between 2237 – 2397 cPs relatively equal in all formulas with pseudoplastic rheological properties. The patchouli oil liquid soaps had antibacterial activity against Staphylococcus aureus ATCC 25923, where F0, F1, F2, F3 and positive control result in inhibition zone diameter of 11.26±2.60 mm, 14.60 ± 2.45mm, 15.51 ± 0.44 mm, 17.97 ± 0.71 mm and 19.00 ± 3.92 mm respectively. Based on the ANOVA one way test, the addition of 1% patchouli oil gave a significant effect on the inhibition zone diameter compared to F0 (without patchouli oil)

scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN RACUN AYAM (Brucea javanica [L.] Merr.) ASAL KALIMANTAN SELATAN

2021 ◽  
Vol 6 (1) ◽  
pp. 59-68
Author(s):  
Sutomo Sutomo ◽  
◽  
Arnida Arnida ◽  
Fahriah Fahriah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Zone Diameter ◽  
Brucea Javanica

Racun ayam is one of the plants which has several activities in its fruits, one of which is an antibacterial. This study aims to determine the antibacterial activity of ethanol extract of racun ayam leaves. The extraction was done by maceration method using ethanol as a solvent. Antibacterial activity assay was carried out on S. aureus, E. coli, and P. aeruginosa bacteria by disk diffusion method at the concentrations of 80, 100, and 120 ppm. The results showed that the ethanol extract of B. javanica leaves had the greatest inhibition zone on E. coli with inhibition zone diameter values ​​at 80, 100, and 120 ppm were 11.7 mm, 14.5 mm and 19.67 mm respectively. Inhibition zones in S. aureus could only be seen at 100 and 120 ppm which had inhibition zone diameters at 1.17 mm and 2.17 mm respectively, whereas in P. aeruginosa they did not have any inhibitory zone values. The results of statistical analysis showed a significance of the inhibition zone diameter values ​​of E. coli bacteria starting at 80 ppm with a sig value of 0.034. Due to a sig value was <0.05, it was categorized as significantly different from the negative control. The bacteria of S. aureus which had ​​<0.05 for sig values only found on 100 ppm and 120 ppm. Bacteria P. aeruginosa which had sig value >0.05 was not significantly different at all concentrations

scholarly journals Antibacterial Activities of Perfume: Combination Flower Magnolia alba, Cananga odorata and Mimusops elengi L, Fixed with Pogostemon cablin Oil

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Rosnani Nasution ◽  
Azalia Izdhihar Azwar ◽  
Hira Helwati ◽  
Marianne
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
E Coli ◽  
Pogostemon Cablin ◽  
Inhibitory Power ◽  
Mimusops Elengi ◽  
Cananga Odorata

Abstract: The making of perfume from the combination Magnolia  alba (M. alba) flower oil, Cananga odorata (C. odorata) and Mimusops elengi L (M. elengi) fixed with Pogostemon cablin oil (P. cablin) have been done. Essential oil of M. alba and C. odorata was distilled by water distillation and extraction essential oil of M. elengi flower using n-hexane evaporated solvent method. Composition perfume through organoleptic test obtained with a ratio of 4% v/v, 4% v/v, and 2% v/v for each essential oil M. alba, C. odorata and M. elengi. Antibacterial activity of the perfume and each of the volatile oil was performed by the disc diffusion method. Antibacterial activity test showed that perfumes were able to inhibit the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, but less active. Antibacterial activity of the perfume was more active against E. coli with an inhibitory zone of 8.3 mm and 39.81% inhibitory power  than S. aureus bacterial 7.3 mm inhibition zone with 37.55% inhibitory power. Perfumes after storage for  one, and two months had better antibacterial activity than before storage with an average inhibitory zone of 11-15 mm and inhibitory power of 50- 68,18%.   Keywords: Antibacterial, E. Coli, C. Odorata, M. alba, M. elengi, P. cablin, , S. aureus  

scholarly journals Antibacterial Activities from Gel Hand Sanitizer of Jatropha curcas Leaves Ethanolic Extract Against Staphylococcus aures ATCC 25923

2020 ◽  
Vol 1 (1) ◽  
pp. 6-11
Author(s):  
Erviana Vernanda Margaretha ◽  
Yohanes Juliantoni ◽  
Dyke Gita Wirasisya
Keyword(s):  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Food Contamination ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Hand Sanitizer

Food borne disease is a disease with symptoms such as nausea, diarrhea and vomiting. The main cause of FBD was food contamination of Staphylococcus aureus. Jarak pagar (Jatropha curcas Linn) leave is a plant that have antibacterial activity against S. aureus because it contains saponin, flavonoid, and tannin. This research aimed to determine the antibacterial activity of the ethanolic extract of Jatropha curcas Linn. leaves, formulated them as gel hand sanitizer, and evaluated the antibacterial activity of the formula. The sample was collected, determined, and extracted with ethanol 70% using maceration, and the phytochemical screening conducted to the concentrate extract. The antibacterial activity test conducted by well diffusion method with concetration 50, 100, dan 150 mg/mL. Then the effective extract group has formulated to gel hand sanitizer. The gel hand sanitizer formula tested the antibacterial activity and physical properties by organoleptic, pH, stability, and spreadablility. The result showed that the extract with concentration 50, 100, dan 150 mg/mL has inhibition zone diameter was 9.9, 15.7, and 16.4 mm, respectively. The gel hand sanitizer of Jatropha curcas Linn extract was formulated by using 100 mg/mL concentration of extract. The gel was physically stable and had inhibition zone diameter was 15.6 mm

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