scholarly journals Uji antibakteri kombinasi ekstrak daun Biduri (Calotropis gigantea L.) dan daun Bandotan (ageratum conyzoides L.)

2020 ◽  
Vol 1 (2) ◽  
pp. 165
Author(s):  
Munira Munira ◽  
Fina Rodisa ◽  
Muhammad Nasir
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Ageratum Conyzoides ◽  
Inhibition Zone Diameter ◽  
Calotropis Gigantea ◽  
Microbiological Test ◽  
Zone Diameter

Background: Biduri leaves (Calotropis gigantea (L.) W.T. Aiton and Bandotan leaves (Ageratum conyzoides L.) are wild growing plants that are believed to be used as an antibacterial.Objectives: This research was conducted to determine the effect of combination ethanolic extract of Biduri and Bandotan leaves against Staphylococcus aureus and Staphylococcus epidermidis.Methods: This research is an experimental by using Completely Randomized Design (CRD) with 4 treatments which is P0 (aquadest), P1 (Biduri leaf extract), P2 (Bandotan leaf extract), and P3 (combination of Biduri and Bandotan leaf extract) with 5 replications. Microbiological test used was the diffusion method.Results: The results of Anova showed that combination of Biduri and Bandotan leaf extract very influential in the growth of Staphylococcus aureus dan Staphylococcus epidermidis. Duncan further test results showed that the largest average inhibition zone diameter in inhibiting Staphylococcus aureus was formed by P2 which is 14.14 mm, significantly different from P1 (9.46 mm) and P3 (11.20 mm), but P1 is not significantly different from P3. Toward Staphylococcus epidermidis, the largest average inhibition zone diameter also formed by P2 which is 17.06 mm that is also significantly different from P1 (10.14 mm) and P3 (12.66 mm), and P1 is not significantly different from P3.Conclusion: The combination of Biduri and Bandotan leaf extract very influential against Staphylococcus aureus and Staphylococcus epidermidis.

scholarly journals EFEKTIVITAS BERBAGAI KONSENTRASI EKSTRAK DAUN KAYU MANIS (Cinnamomum burmannii [Ness.] BI) TERHADAP DIAMETER ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis

Jurnal Biota ◽  
2018 ◽  
Vol 4 (1) ◽  
pp. 12-18
Author(s):  
Moh. Syaifuddin Qomar ◽  
Moch. Agus Krisno Budiyanto ◽  
Sukarsono Sukarsono ◽  
Sri Wahyuni ◽  
Husamah Husamah
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Skin Infections ◽  
Inhibitory Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Cinnamomum Burmannii ◽  
Biology Laboratory

Staphylococcus epidermidis is one of the bacteria that can cause infectious diseases. In general Staphylococcus epidermidis can cause diseases of swelling (abscess) such as skin infections or acne. Cinnamon (Cinnamomum burmannii [Ness.] BI) leaf extract has a substance which gives antimicrobial effects that can be used as an inhibitor of the Staphylococcus epidermidis bacteria’s growth because it contains substances such as essential oils, flavonoids, saponins, tannins, and alkaloids. The aim of this research is to analyse the effect of the concentration of cinnamon leaf extract and to determine the concentration of extract which has the best influence to the inhibition zone diameter of Staphylococcus epidermidis bacteria. This research was conducted in Biology Laboratory of University of Muhammadiyah Malang, on 17-18 May 2017. The results showed that there was an effect of giving various concentrations of cinnamon leaf extract to the inhibition zone diameter of Staphylococcus epidermidis bacteria’s growth. The concentration of cinnamon leaf extract which has the best influence to the inhibitory zone diameter of Staphylococcus epidermidis bacteria is 100% with the mean of inhibitory zone diameter 15.16 mm.

scholarly journals Pengaruh lama maserasi daun ketapang merah (Terminalia Catappa L.) terhadap daya hambat Staphylococcus aureus dan Escherichia coli

2021 ◽  
Vol 2 (2) ◽  
pp. 158
Author(s):  
Berwi Fazri Pamudi ◽  
Munira Munira ◽  
Rizky Amalia Saha ◽  
Muhammad Nasir
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Terminalia Catappa ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Background: Bengal Almond leaves (Terminalia catappa L.) contain alkaloids, saponins, tannins, polyphenols, quinones, flavonoids and triterpenoids that can function as antibacterial.Objectives: This research was conducted to determine the effect of maceration time of red ketapang leaves (Terminalia catappa L.) on the growth of Staphylococcus aureus and Escherichia coli. Methods: This study was experimental using a completely randomized design (CRD) consisting of 6 treatments, that P0 (aquades), P1 (red ketapang leaf extract with 1 day maceration time), P2 (2 days maceration) P3 (3 days maceration) , P4 (4 days maceration) and P5 (5 days maceration) with 4 replications.Results: Anova test showed that macerated red ketapang leaf extract significantly affected the growth of Staphylococcus aureus and Escherichia coli (p= 0.000). Duncan's further test showed that the largest inhibition zone diameter in Staphylococcus aureus was formed by P2 (2 days maceration time) which was 18.62 mm and there was no significant difference between treatments. Meanwhile, the largest inhibition zone diameter against Escherichia coli was formed by P1 (1 day maceration time) which was 20.25 mm and there was a significant difference between treatments. Conclusion: Ketapang red leaf extract with different maceration times can inhibit the growth of Staphylococcus aureus and Escherichia coli.

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals AKTIVITAS ANTIBAKTERI ISOLAT KAPANG ENDOFIT DARI KULIT NANAS (Ananas comosus (L.) Meer)

2021 ◽  
Vol 4 (02) ◽  
pp. 20-30
Author(s):  
Siti Hamidatul Aliyah ◽  
Musfirotun Musfirotun ◽  
Nur Antriana
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Ananas Comosus ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pineapple Peel ◽  
Living Organisms

Pineapple contains an enzyme called bromelain which is can be used as antiseptic of mouth, antibacterial, antifungal, and disinfectant. Endophytic mold is a microbe that forms colonies in healthy tissues of living organisms, generally, endophytic microbes do not cause harmful symptoms in the tissue of their host. This study aims to isolate the endophytic shell origin of pineapple peel that has acted as an antibacterial. A total of 3 endophytic capsules, Ac-I, Ac-II and Ac-III were isolated from pineapple skin using PDA media. The three isolates were purified and microscopic examinations were performed. Antibacterial testing was performed by fermentation to produce supernatant, then tested using disc method (Kirby-Bauer method) with Staphylococcus aureus and Escerichia coli test bacteria. The 3 isolates obtained only 1 isolate Ac-III isolates that have activity as antibacterial, with the inhibition zone diameter in bacterium Staphylococcus aureus 7.65 mm while in the bacterium Escerichia coli 6,9 mm.

scholarly journals Effects of Different Solvents to Extract Phytochemical Materials of Rhazya stricta Against Salmonella typhimurium Activity

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli Nasab ◽  
Hasan Ahmadi ◽  
Mehrangiz Ghafari
Keyword(s):  
Medicinal Plants ◽  
Growth Inhibition ◽  
Salmonella Typhimurium ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Average Growth ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Rhazya Stricta ◽  
Growth Inhibition Zone

Background: The use of plant extracts prepared from medicinal plants is common in the developing countries. Meanwhile, due to the resistance of microorganisms to antibiotics, researchers are looking to discover new antimicrobials. Medicinal plants play an essential role in this regard. Objectives: This study was aimed at investigating the antimicrobial properties of various extracts of Rhazya stricta plant against Salmonella typhimurium. Methods: Methanolic, aqueous, ethanolic, hydroalcoholic, and ethyl acetate extracts of R. stricta were prepared. S. typhimurium strains were isolated from poultry feces. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of this plant. The diameter of the growth inhibition zone using was determined using Whatman paper. Results: The lowest MIC against S. typhimurium was obtained from the hydroalcoholic solvent with 3.1 ppm. The most effective extraction solvent to inhibit the growth of S. typhimurium was the hydroalcoholic type with an average growth inhibition zone diameter of 12.25 mm, followed by the ethanolic extract with a growth inhibition zone diameter of 12.12 mm. Conclusions: According to the results of research and increasing resistance to synthetic antibacterial substances, it seems that R. stricta plant with the help of hydroalcoholic solvent, can be considered an effective plant in eliminating some bacteria, including S. typhimurium.

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

Karakteristik Fisik dan Aktivitas Antibakteri Sabun Cair Minyak Nilam (Pogostemon cablin Benth.) yang Berbasis Surfaktan Sodium Lauril Eter Sulfat

2020 ◽  
pp. 1-10
Author(s):  
Yuni Anggraeni ◽  
Fakhrun Nisa' ◽  
Ofa Suzanti Betha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Foam Height ◽  
Inhibition Zone ◽  
Positive Control ◽  
Centrifuge Test ◽  
Liquid Soap ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pogostemon Cablin

Patchouli oil obtained from patchouli plants (Pogostemon cablin Benth.) has been widely used as an antibacterial agent in liquid and solid soap preparation. In this research, patchouli oil was added to liquid soap formula which use sodium lauryl ether sulphate (SLES) surfactant. The purpose of this study was to determine the physical characteristics and antibacterial activity of patchouli oil liquid soaps based on SLES surfactant. The Liquid soaps were prepared into four formulas with variation of patchouli oil concentration that were F0 (0.00% w/w), F1 (0.05% w/w), F2 (0.50% w/w), and F3 (1.00% w/w). The liquid soaps were evaluated including organoleptic, microscopic property, physical stability by using centrifuge test, pH, foam height and stability, viscosity and rheology, and antibacterial activity against Staphylococcus aureus ATCC 25923 by using disc diffusion method and chloramphenicol as a positive control. The results showed that all liquid soap formulas had following characteristics: translucent except F3 slightly murky, homogen, stable after centrifuge test, pH between 6.55 – 6.66 that were no significantly different and met SNI standards of liquid soap, foam height between 2.67 – 3.23 cm that were no significantly different and stable after 5 minutes of testing, viscosity between 2237 – 2397 cPs relatively equal in all formulas with pseudoplastic rheological properties. The patchouli oil liquid soaps had antibacterial activity against Staphylococcus aureus ATCC 25923, where F0, F1, F2, F3 and positive control result in inhibition zone diameter of 11.26±2.60 mm, 14.60 ± 2.45mm, 15.51 ± 0.44 mm, 17.97 ± 0.71 mm and 19.00 ± 3.92 mm respectively. Based on the ANOVA one way test, the addition of 1% patchouli oil gave a significant effect on the inhibition zone diameter compared to F0 (without patchouli oil)

scholarly journals Effect of Spoilage on the Antimicrobial Potential and Phytochemical Components of Ginger and Garlic

2021 ◽  
pp. 63-66
Author(s):  
Osaro-Matthew R. C. ◽  
Itaman V. O. ◽  
Kogonye J.
Keyword(s):  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Antimicrobial Potential ◽  
Test Organisms ◽  
Aspergillus Sp ◽  
Phytochemical Components

This study evaluated the effect of microbial spoilage on the phytochemical component and antimicrobial potential of Garlic (Alliums Sativum) and Ginger (Zingiber offinale). The antimicrobial activities of both spoilt and un-spoilt Ginger and Garlic were assessed by measuring the inhibition zone diameter using agar well diffusion method. The result showed that both spoilt and un-spoilt samples were active against all test organisms with ethanolic extract of un-spoilt Garlic showing the highest activity against Staphylococcus sp. and Aspergillus sp with mean inhibition zone diameter (21.00mm and 21.50mm) respectively. Similarly the ethanolic extract of un-spoilt Ginger exhibited greatest activity against Staphylococcus sp. and Aspergillus sp with mean inhibition zone diameter (20.00Smm and 20.00mm) respectively. The preliminary phytochemical screening of the samples revealed the absence of saponin in spoilt samples of both Ginger and Garlic while phenol and flavonoid became more prominent in the both spoilt samples. This study confirms that microbial degradation of some plant extracts does not affect the antimicrobial potential and suggest usage of these products even if spoilage is noticed.

scholarly journals Antibacterial Activity of Ethanolic Extract of Sangkareho (Callicarpa longifolia Lam.) against Staphylococcus epidermidis

2018 ◽  
Vol 1 (2) ◽  
pp. 85-88 ◽  
Author(s):  
Susi Novaryatiin ◽  
Asma'ul Ayuriska Sari ◽  
Evi Mulyani
Keyword(s):  
Antibacterial Activity ◽  
Traditional Medicine ◽  
Staphylococcus Epidermidis ◽  
Skin Disease ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Disc Diffusion ◽  
Central Kalimantan ◽  
Inhibition Zone Diameter ◽  
Diffusion Technique

One of the plants believed as traditional medicine by the Dayak tribes in Central Kalimantan was Sangkareho (Callicarpa longifolia Lam.). This plant was usually used as traditional medicine by society in Puruk Cahu, Murung Raya Regency, Central Kalimantan and empirically believed could cure skin disease such as wound infection. This study was aimed to determine the antibacterial activity of ethanolic extract of Sangkareho (Callicarpa longifolia Lam). Antibacterial activity of ethanolic extract of Sangkareho was performed using disc diffusion technique, with four variations of concentration of 1%, 5%, 10%, and 15%. In this present study showed that ethanolic extract of Sangkareho has antibacterial activity against Staphylococcus epidermidis, wherein the resulting inhibition zone diameter was 7.3�0.8 mm, 8.3�1.2 mm, 10.5�0.5 mm, and 12.2�0.7 mm, respectively. Further research is needed to obtain an antibacterial activity of the fraction of Sangkareho.

scholarly journals Antibacterial Activities from Gel Hand Sanitizer of Jatropha curcas Leaves Ethanolic Extract Against Staphylococcus aures ATCC 25923

2020 ◽  
Vol 1 (1) ◽  
pp. 6-11
Author(s):  
Erviana Vernanda Margaretha ◽  
Yohanes Juliantoni ◽  
Dyke Gita Wirasisya
Keyword(s):  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Food Contamination ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Hand Sanitizer

Food borne disease is a disease with symptoms such as nausea, diarrhea and vomiting. The main cause of FBD was food contamination of Staphylococcus aureus. Jarak pagar (Jatropha curcas Linn) leave is a plant that have antibacterial activity against S. aureus because it contains saponin, flavonoid, and tannin. This research aimed to determine the antibacterial activity of the ethanolic extract of Jatropha curcas Linn. leaves, formulated them as gel hand sanitizer, and evaluated the antibacterial activity of the formula. The sample was collected, determined, and extracted with ethanol 70% using maceration, and the phytochemical screening conducted to the concentrate extract. The antibacterial activity test conducted by well diffusion method with concetration 50, 100, dan 150 mg/mL. Then the effective extract group has formulated to gel hand sanitizer. The gel hand sanitizer formula tested the antibacterial activity and physical properties by organoleptic, pH, stability, and spreadablility. The result showed that the extract with concentration 50, 100, dan 150 mg/mL has inhibition zone diameter was 9.9, 15.7, and 16.4 mm, respectively. The gel hand sanitizer of Jatropha curcas Linn extract was formulated by using 100 mg/mL concentration of extract. The gel was physically stable and had inhibition zone diameter was 15.6 mm

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