scholarly journals Early Study on Embryogenesis O.woworae at Different Salinities

2021 ◽  
Vol 21 (2) ◽  
pp. 343
Author(s):  
Fani Savitri Agatha ◽  
Mustahal Mustahal ◽  
Mas Bayu Syamsunarno ◽  
Muh. Herjayanto
Keyword(s):  
Experimental Methods ◽  
Hatching Rate ◽  
Basic Information ◽  
Optimal Salinity ◽  
Cultivation System ◽  
Hatching Time ◽  
Endemic Fish ◽  
Optimum Salinity

Oryzias woworae is an endemic fish of Sulawesi. This endemic fish naturally had faced endangered illegal and unlawful capture, therefore it is needed to be protected and conserved by a cultivation system. This research aims to provide basic information related to the cultivation of O. woworae to know the optimum salinity of hatching media and its influence on the hatchability of eggs and the length of time hatching O. woworae. This research was conducted using experimental methods and presented descriptively. The treatment used in this study is 0 ppt, 2-4 pt, 6-8 ppt, 10-12 ppt. The results showed that O. woworae eggs can hatch in salinity 0 ppt until 10-12 ppt and the best salinity for the length of hatching time is treatment of 2-4 ppt which is 7th day 14 hours 20 minutes. Treatment 0 ppt, 2-4 ppt and 6-8 ppt can reach 100%. This can be concluded that the optimum salinity of the hatching rate of Oryzias woworae until 6-8 ppt, but in treatment 10-12 ppt hatchability only gets 80% as some embryos become shrinking. Hatchability per day shows the treatment of 2-4 ppt is the best treatment, on the 7th day as much as 40% and on the 8th day as much as 40%. The optimal salinity for hatching O. woworae eggs is  2-4 ppt treatment, salinity that can expedite the length of hatching time and produce  hatching rate by 100%.  

scholarly journals Osmoadaptation Strategy of the Most Halophilic Fungus, Wallemia ichthyophaga, Growing Optimally at Salinities above 15% NaCl

2013 ◽  
Vol 80 (1) ◽  
pp. 247-256 ◽  
Author(s):  
Janja Zajc ◽  
Tina Kogej ◽  
Erwin A. Galinski ◽  
José Ramos ◽  
Nina Gunde-Cimerman
Keyword(s):  
Biomass Production ◽  
Osmotic Shock ◽  
Model Organism ◽  
Salinity Range ◽  
Growth Media ◽  
Optimal Salinity ◽  
Content Type ◽  
Batch Cultures ◽  
Specific Growth Rates ◽  
Optimum Salinity

ABSTRACTWallemia ichthyophagais a fungus from the ancient basidiomycetous genusWallemia(Wallemiales, Wallemiomycetes) that grows only at salinities between 10% (wt/vol) NaCl and saturated NaCl solution. This obligate halophily is unique among fungi. The main goal of this study was to determine the optimal salinity range for growth of the halophilicW. ichthyophagaand to unravel its osmoadaptation strategy. Our results showed that growth on solid growth media was extremely slow and resulted in small colonies. On the other hand, in the liquid batch cultures, the specific growth rates ofW. ichthyophagawere higher, and the biomass production increased with increasing salinities. The optimum salinity range for growth ofW. ichthyophagawas between 15 and 20% (wt/vol) NaCl. At 10% NaCl, the biomass production and the growth rate were by far the lowest among all tested salinities. Furthermore, the cell wall content in the dry biomass was extremely high at salinities above 10%. Our results also showed that glycerol was the major osmotically regulated solute, since its accumulation increased with salinity and was diminished by hypo-osmotic shock. Besides glycerol, smaller amounts of arabitol and trace amounts of mannitol were also detected. In addition,W. ichthyophagamaintained relatively small intracellular amounts of potassium and sodium at constant salinities, but during hyperosmotic shock, the amounts of both cations increased significantly. Given our results and the recent availability of the genome sequence,W. ichthyophagashould become well established as a novel model organism for studies of halophily in eukaryotes.

scholarly journals Preliminary Study Rearing of Oryzias sp. from Tunda Island, Indonesia, Under Laboratory Condition

2019 ◽  
pp. 24-34
Author(s):  
Muh. Herjayanto ◽  
Annisa Misykah Mauliddina ◽  
Esa Rama Widiyawan ◽  
Nugroho Agung Prasetyo ◽  
Lukman Anugrah Agung ◽  
...  
Keyword(s):  
Laboratory Condition ◽  
Model Organism ◽  
Ornamental Fish ◽  
Fish Mortality ◽  
Controlled Environment ◽  
Hatching Rate ◽  
Basic Information ◽  
Hatching Process ◽  
Preliminary Study

Oryzias sp. from Tunda Island, Indonesia has the potential as a model organism in laboratories and also ornamental fish. Basic information regarding maintenance during adaptation post-transportation from its habitat in nature, embryo incubation and maintenance of larvae is needed as a basis for breeding activities. The results showed that Oryzias sp. from Tunda Island can adapt, spawning and produce a total 262 eggs (diameter 0.88-1.02 mm) in a controlled environment. Eggs from spawning in nature begin to hatch on the 11th incubation day, the hatching peak occurs on the 13th day with a hatching process 4-9 days at 25-27oC. The hatching rate ranges from 57.14-100% and the survival of 5 days old lavae after hatching is 72.72-100.00%. The transportation technique in this study has not been able to suppress fish mortality during post-transportation rearing.    

scholarly journals Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens

DEPIK ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 235-242
Author(s):  
Mustaqim Mustaqim ◽  
Kartini Eriani ◽  
Erlangga Erlangga ◽  
Rachmawati Rusyidi
Keyword(s):  
Water Quality ◽  
Fish Larvae ◽  
Fertilization Rate ◽  
Quality Parameters ◽  
Betta Splendens ◽  
Effect Of Temperature ◽  
Hatching Rate ◽  
Egg Hatchability ◽  
Hatching Time ◽  
Randomized Design

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak.  Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

The Design of Temperature and Humidity Control System in Multi Incubators Based on Fuzzy Control

2011 ◽  
Vol 130-134 ◽  
pp. 2400-2403
Author(s):  
Rui Lan Wang
Keyword(s):  
Fuzzy Control ◽  
Control Method ◽  
Control Algorithms ◽  
Hatching Rate ◽  
Hatching Time ◽  
Control Precision ◽  
Temperature And Humidity ◽  
High Control ◽  
Coupling Characteristics ◽  
Control Accuracy

Hatching systems requiring high control accuracy of temperature and humidity. Particularly temperature, small temperature change will significantly affect hatching time and hatching rate；and temperature and humidity is the controlled amount of interaction between two. Due to the coupling characteristics of temperature and humidity is difficult to establish a precise mathematical model, caused uncertainty in its control. Fuzzy control method witch proposed in this article will not only improve control precision, and to improve the robustness of control algorithms and adaptive capacity.

scholarly journals Pemijahan Ikan Endemik Pelangi Arfak (Melanotaenia Arfakensis) Secara Alami

2021 ◽  
Vol 2 ◽  
pp. 39-43
Author(s):  
Vicky Arnold Mofu ◽  
Bayu Pranata ◽  
Marhan Manaf
Keyword(s):  
Survival Rate ◽  
Larval Survival ◽  
Hatching Rate ◽  
West Papua ◽  
The Status ◽  
Endemic Fish ◽  
Lake Systems

Arfak rainbowfish (Melanotaenia arfakensis) is one of the endemic fish of West Papua that can be found in river and lake systems in the Manokwari Regency. Currently, the status of the arfak rainbowfish is nearly extinct. Therefore, this study aimed to know the hatching rate and survival of arfak rainbowfish larvae that were naturally spawned. The results showed that the hatchability of arfak rainbowfish eggs reached 96.9% in A1 aquarium and 96.4% in A2 aquarium. The survival rate of arfak rainbowfish larvae in the A1 aquarium was 67.5%, while the A2 aquarium was 58.7%. The larval survival rate was relatively low. Therefore, further research is needed to increase the survival rate of arfak rainbowfish larvae. 

scholarly journals Effects of ventilation programme and eggshell thickness on hatchability rate and hatching time of broiler eggs

2021 ◽  
Vol 51 (2) ◽  
pp. 205-211
Author(s):  
N. Okur ◽  
S.A. Eratalar
Keyword(s):  
Carbon Dioxide ◽  
Significant Interaction ◽  
Hatching Rate ◽  
Eggshell Thickness ◽  
Hatching Time ◽  
Carbon Dioxide Co2 ◽  
Embryonic Death

The aim of the research was to determine whether enrichment of the atmosphere in an incubator with carbon dioxide (CO2) and oxygen (O2) and eggshell thickness (EST) affected embryonic death (ED), hatchability of fertile eggs (HFE) and hatching time (HT). A total of 320 Ross 308 eggs were used and the experiment was repeated twice. Eggshell thickness was classified as thin (<31 μm), medium (31 - 32 μm) and thick (>32 μm). The incubators were operated with their internal atmosphere enriched with CO2 (1.57% CO2; 20.23% O2) or O2 (0.50% CO2; 22.44% O2). Embryonic death, HFE and HT data were monitored at three periods, namely early (<486 hours), middle (486 - 492 hours), and late (492 - 510 hours). Early ED, late ED and hatchability of fertile eggs were not affected by EST or by the incubator’s internal atmosphere (P >0.05). Thus, O2 supplementation to the incubator was deemed unnecessary at 822 m altitude. There was a highly significant interaction between EST and HT. Eggs with shells 31 to 32 μm thick hatched at an appreciably greater rate between 486 and 492 hours of incubation (17%) than eggs with thicker (0.6%) or thinner (0.4%) shells in both the CO2 and O2 enriched atmospheres. The hatching rate was significantly higher in the eggs with an intermediate EST than in thick-shelled eggs. A greater proportion of eggs hatched at the late HT as opposed to earlier, regardless of EST.

scholarly journals PLANNING ON HATCHING TIME OF GROUPER EGGS THROUGH DIFFERENT INCUBATION TEMPERATURES

2010 ◽  
Vol 2 (2) ◽  
Author(s):  
Regina Melianawati ◽  
Philip Teguh Imanto ◽  
Made Suastika
Keyword(s):  
Embryonic Development ◽  
Incubation Time ◽  
Incubation Temperature ◽  
The Body ◽  
Development Pattern ◽  
Hatching Rate ◽  
Optimum Temperature ◽  
Hatching Time ◽  
Tiger Grouper ◽  
Lower Temperature

Groupers were known as a high economically marine commodity and in order to support groupers production, the seed availability was the most important. Eggs are still as limited factor in hatchery production, for this reason the success of eggs transportation is one as base of successful production of seed. Planning on hatching time of eggs through different incubation temperature was an option to solve that problem. This experiment was aimed to find out the optimum temperature for groupers eggs and the minimum temperature to arrange incubation time and to plan the hatching time. Fertilized eggs were incubated into three beaker glasses of 1 liter in volume with the density of ± 250 eggs/liter. The incubation was done under laboratory condition at controlled temperature, i.e. (A) 21-22 ºC, (B) 24-25 ºC, (C) 27-28 ºC and (D) 30-31 ºC. The eggs that used were including orange spotted grouper (Epinephelus coiodes), brown marbled grouper (E. microdon), tiger grouper (E. fuscoguttatus) and humpback grouper (Cromileptes altivelis). Investigated variables were embryonic development pattern, incubation time and hatching rate. The result showed that the eggs incubated in temperature range of 24-31°C had the normal sequence of embryonic development pattern, but in temperature of 21-22°C performed irregular sequence and the embryonic development stopped at blastula or gastrula stage or even the eggs could still develop but the body of hatched larvae were abnormal. In lower temperature incubation, the incubation time was longer and the hatching rate of eggs was lower than those in higher temperature. Therefore the optimum temperature for incubation of orange spotted grouper, marbled grouper, tiger grouper and humpback grouper eggs ranged between 24-31 ºC, while the lowest possible temperature was 24 ºC.Keywords: incubation temperature, embryonic development pattern, grouper eggs, hatching rate

scholarly journals Incubation of Oryzias woworae Eggs at Different Temperature on Embryo Development and Hatching Performance

2021 ◽  
Vol 21 (2) ◽  
pp. 315
Author(s):  
Nisa Nafiyanti ◽  
Mustahal Mustahal ◽  
Mas Bayu Syamsunarno ◽  
Muh. Herjayanto
Keyword(s):  
Incubation Temperature ◽  
Economic Value ◽  
Fish Farming ◽  
Hatching Rate ◽  
Water Medium ◽  
Optimal Temperature ◽  
Egg Incubation ◽  
Hatching Time ◽  
Hatching Eggs ◽  
Incubation Temperatures

Oryzias woworae is one of Muna Island's endemic fish, Sulawesi, which needs to be developed in cultivation and can be used as ornamental fish with high economic value. However, information on fish farming is not yet well available, especially at egg incubation temperatures. This study aims to determine the optimal temperature of the incubation water medium for the hatching performance of O. woworae eggs. The study used experimental methods with the treatment of differences in egg incubation temperatures at 24-26°C (temperature fluctuations in the laboratory), 24°C, 28°C and 32°C. The research parameters were analyzed descriptively, namely embryogenesis, hatching time, and hatching rates. The results showed that differences in incubation temperature affected the embryogenesis of O. woworae eggs. The obtained fastest time for hatching eggs O. woworae at a temperature of 32°C with six days 5 hours 49 minutes and 100% hatching. The egg incubation temperature 24-26°C is the lowest hatching time and hatching rate, nine days 11 hours 38 minutes and 53.33%. The use of 32°C in the incubation medium is the optimal temperature to accelerate embryogenesis and the hatching rate of O. woworae eggs.

scholarly journals Quality of Adherence to the ARRIVE Guidelines in the Material and Methods Section in Studies Where Swine Were Used as Surgical Biomodels: A Systematic Review (2013–2018)

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 947
Author(s):  
Jilma Alemán-Laporte ◽  
Gilbert Alvarado ◽  
Mariana SA Garcia-Gomes ◽  
Ana Tada Fonseca Brasil Antiorio ◽  
Marco Zúñiga-Montero ◽  
...  
Keyword(s):  
Systematic Review ◽  
Sample Size ◽  
Animal Welfare ◽  
Sample Size Calculation ◽  
Surgical Techniques ◽  
Experimental Methods ◽  
Basic Information ◽  
Quality Reporting ◽  
Excellent Tool

Over the last two decades, pigs have become animal biomodels widely used for the investigation and practice of surgical techniques because of their great physiological and anatomical similarities to humans. Even though many of these studies must be carried out later in humans, the description of basic information is limited, making exact repetitions of the reported experimental methods impossible. In this review, 108 studies from 2013 to 2018 were considered to determine the quality of adherence to the ARRIVE guidelines in the reports of the methodologies. The majority of the studies lacked the details recommended in the ARRIVE guidelines regarding data directly related to the welfare of animals undergoing surgery and those about anesthetic protocols and analgesics. Information related to sample size calculation and housing and husbandry conditions was also very limited. We believe that the ARRIVE guidelines are an excellent tool for good-quality reporting. We encourage scientists to consistently use them as a tool to improve the quality of their scientific reports and, consequently, ensure animal welfare.

scholarly journals Cocoon production and hatching rates of Branchiura sowerbyi Beddard (Oligochaeta: Tubificidae)

2008 ◽  
Vol 25 (1) ◽  
pp. 16-19 ◽  
Author(s):  
Haroldo L. S. Nascimento ◽  
Roberto da G. Alves
Keyword(s):  
Elevated Temperatures ◽  
Hatching Rate ◽  
Distilled Water ◽  
Cocoon Production ◽  
Branchiura Sowerbyi ◽  
Hatching Time ◽  
Clay Sediment ◽  
Different Populations ◽  
Biological Aspects ◽  
Hatching Rates

Branchiura sowerbyi Beddard, 1892 is a Tubificidae species abundant in tropical limnic environments and associated with sediments rich in organic matter and elevated temperatures. The present study aimed at obtaining information on laying and hatching rates of B. sowerbyi cocoons under laboratory conditions. In order to do this, specimens of B. sowerbyi were placed in beakers (250 ml) containing clay sediment and distilled water and kept at 25ºC. The obtained cocoons were placed in beakers of 100 ml and kept in conditions similar to the mature individuals. Fifty seven cocoons were collected, with a mean of 0.13 cocoons·individual-1·day-1 and 1.21 (±0.08) eggs·cocoon-1. Hatching time was approximately two weeks and hatching rate was 44.93%. The results obtained were different from the ones found by other authors for B. sowerbyi. According to the literature, different populations of the same species can present distinct biological aspects, such as the number of cocoons, hatching rates and growth.

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