Pengaruh Pemberian Ekstrak Kencur (Kaempferia galanga L.) terhadap Peningkatan Zona Hambat Pertumbuhan Bakteri Staphylococcus aureus

The prevalence of infectious diseases in Indonesia is still relatively high, for example Infection caused by Staphylococcus aureus. Indonesia has many traditional plants that can be used for herbal treatments containing tannins, flavonoids, and essential oils which have relatively high antibacterial activity by forming inhibitory zones in the Staphylococcus aureus bacteria which are the cause of skin infections food poisoning to systemic infections. Thereforekencur can be used as an alternative medicine. This study was intented to determine the effect of giving kencur extract (Kaempferia galanga L.) In inhibiting the growth of Staphylococcus aureus bacteria. This study was experimental laboratory used plate diffusion method of “Postest Ony Control Group Design” design. There were 7 groups, 5 treatment groups and 2 control groups. The concentration of treatment group was 100%, 50%, 25%, 12,5%, 6,25% while for positive control using amoxicillin 25μg and aquadest as negative control. The bacterial used were Staphylococcus aureus. The results showed that kencur extract (Kaempferia galanga L.) had an antibacterial effect in inhibiting the growth of Staphylococcus aureus bacteria.With a value of p = 0.001 or p <α. kencur extract (Kaempferia galanga L.) has an antibacterial effect in inhibiting the growth of Staphylococcus aureus bacteria.

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PENGARUH EKSTRAK AKAR ENCOK TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v4i2.50 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Igede Sudarmanto

Keyword(s):

Staphylococcus Aureus ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Statistic Analysis ◽

Control Group Design ◽

Inhibition Zone Diameter ◽

Group Design ◽

The Mean

Background: Resistance is defined as growth inhibition of bacteria with antibiotics. Staphylococcus aureus bacteria is known to be resistant to gentamicin and methicillin. Staphylococcus aureus can cause various infection of the skin, injuries, and system. Methods: This research is true-experimental with Posttest-Only Control Group Design and using Kirby-Bauer method by means of five concentration (20, 40, 60, 80, dan 100%). The negative control uses sterile distilled water, and the positive control uses Vancomycin 30 µg. Result: This research showed the mean of inhibition zone diameter of each concentration consecutively 16 mm, 20 mm, 22 mm, 23 mm, and 23 mm. Oneway ANOVA statistic analysis showed that the value of p is 0,000 that mean there is an influence of encok roots extract to Staphylococcus aureus growth. Conclusion: There is an influence of encok roots extract to Staphylococcus aureus growth. Seen from its ability to inhibit the growth of Staphylococcus aureus.

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The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽

10.30649/denta.v10i1.37 ◽

2016 ◽

Vol 10 (1) ◽

pp. 71

Author(s):

Almira Fa’Izah ◽

Istien Wardani ◽

Diana Soesilo

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Control Group ◽

Oral Diseases ◽

Clear Zone ◽

Control Group Design

Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.

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Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽

10.35790/eg.4.2.2016.14452 ◽

2016 ◽

Vol 4 (2) ◽

Author(s):

Oktovianus Pormes ◽

Damajanty H.C. Pangemanan ◽

Michael A. Leman

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Amaranthus Hybridus ◽

Group Design ◽

Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

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Uji daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri Staphylococcus aureus

Jurnal e-Biomedik ◽

10.35790/ebm.7.2.2019.23876 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Mayangsari P. Rahayu ◽

Damajanty H. C. Pangemanan ◽

Christy N. Mintjelungan

Keyword(s):

Staphylococcus Aureus ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Zone Of Inhibition ◽

Control Group Design ◽

Group Design ◽

Wide Range ◽

Post Test

Abstract: Mouthwash could inhibit the growth of Staphylococcus aureus bacteria in the oral cavity. Albeit, the most widely used today is mouthwash containing clorhexidine that has side effects in prolonged use. Therefore, it is necessary to find new agents as an alternative antibacterial, especially against Staphylococcus aureus. Squid ink is one of the best known seafood used as alternative medicine which has a wide range of therapeutic applications. This study was aimed to evaluate the inhibitory effect of squid ink extract (Loligo sp) on the growth of S. aureus. This was a true experimental study with a post test only control group design. We used modified Kirby-Bauer method using filter papers. Ciprofloxacin antibacterial was used as the positive control and aquadest as the negative control. Extract of squid ink (Loligo sp) and stock of pure bacteria S. aureus bacteria were prepared. The results showed that mean of zone of inhibition of the squid ink extract (Loligo sp) was 11.22 mm which was less than the zone of inhibition of ciprofloxacin. In conclusion, the squid ink extract (Loligo sp) had a moderate inhibitory effect (Himedia category) on the growth of Staphylococcus aureus.Keywords: extract of squid ink (Loligo sp), Staphylococcus aureus, inhibitory effectAbstrak: Salah satu cara penanganan bakteri Staphylococcus aureus dalam rongga mulut ialah dengan menggunakan obat kumur. Yang banyak digunaakan saat ini yaitu obat kumur yang mengandung clorhexidine dengan efek samping bila digunakan secara berkepanjangan. Oleh karena itudiperlukan penelitian terhadap agen baru sebagai alternatif antibakteri khususnya terhadap bakteri Staphylococcus aureus. Tinta cumi-cumi merupakan salah satu hasil laut yang dikenal dalam dunia pengobatan alternatif serta memiliki jangkauan yang luas pada aplikasi terapeutik. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumuhan bakteri S. aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan kertas saring. Kontrol positif menggunakan antibakteri siprofloksasin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak tinta cumi-cumi (Loligo sp) dan stok bakteri murni S. aureus. Hasil penelitian mendapatkan bahwa diameter rerata zona hambat dari ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri S. aureus sebesar 11,22 mm namun diameter tersebut lebih kecil daripada diameter zona hambat siprofloksasin. Simpulan penelitian ini ialah ekstrak tinta cumi-cumi (Loligo sp) memiliki daya hambat kategori sedang (Himedia) terhadap pertumbuhan bakteri S. aureus.Kata kunci: tinta cumi-cumi (Loligo sp), bakteri Staphylococcus aureus, daya hambat

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KALANDUYUNG (Guazuma ulmifolia Lam.) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS DENGAN METODE DIFUSI CAKRAM (KIRBY-BAUER)

Anterior Jurnal ◽

10.33084/anterior.v17i2.12 ◽

2018 ◽

Vol 17 (2) ◽

pp. 136-143

Author(s):

Adelgrit Trisia ◽

Regina Philyria ◽

Angeline Novia Toemon

Keyword(s):

Staphylococcus Aureus ◽

Ethanol Extract ◽

Inhibition Zone ◽

Lung Parenchyma ◽

Positive Control ◽

Diffusion Method ◽

Control Group ◽

Control Group Design ◽

Guazuma Ulmifolia ◽

One Way Anova

Pneumonia is an infectious lung parenchyma, respiratory bronchioles, and alveolus, caused by Staphylococcus aureus. Kalanduyung (Guazuma ulmifolia Lam.) is an herb plant from Central Borneo which people believe has many benefits, which one is curing pneumonia. To identify the antibacterial activity from the ethanol extract of Kalanduyung leaves to the Staphylococcus aureus growth. This research was experimental with posttest-only control group design. Ethanol extract of Kalanduyung leaves in 20%, 40%, 60%, and 80% concentrations were tested for antibacterial capability against Staphylococcus aureus with Kirby Bauer disc diffusion method, with Amoxicillin as the positive control and 10% Dimethyl Sulfoxide. This data was statistically analyzed with One Way Anova. Ethanol extract of Kalanduyung leaves can inhibit Staphylococcus aureus with each inhibition zone diameter was 6.875 mm, 8.5 mm, 10.175 mm, and 14.925 mm. The ethanol extract of Kalanduyung leaves can inhibit the growth of Staphylococcus aureus bacteria.

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PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (Allium sativum Linn) TERHADAP ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis secara in vitro

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v20i1.35 ◽

2019 ◽

Vol 20 (1) ◽

pp. 28-31

Author(s):

AKBAR DIOSASQIA PUTRA

Keyword(s):

Staphylococcus Epidermidis ◽

Allium Sativum ◽

Antibacterial Effect ◽

Negative Control ◽

Garlic Extract ◽

Diffusion Method ◽

Control Group ◽

High Morbidity ◽

Control Group Design

Background:Infectious diseases are the main cause of high morbidity and mortality rates, especially in developing countries such as Indonesia.Indonesia is abundant with plants that can be used as herbal medicine. Garlic is known as having antibacterial elements, one of them is for Staphylococcus epidermidis which is one cause of dermal disease. This disease is often ignored in Indonesia because the price of the drug is not affordable by the community. So that garlic should be used as an alternative medicineObjective: This study aims to determine the antibacterial effect of garlic extract (Allium sativum L.) retarding the growth of Staphylococcus epidermidis.Methods: This study is experimental laboratory using plate diffusion method of "Postest Ony Control Group Design" design. There are 7 groups, 5 treatment groups and 2 control groups. The concentration of treatment group was 100%, 50%, 25%, 12,5%, 6,25% while for positive control using chloramphenicol and aquadest as negative control. The bacteria used were Staphylococcus epidermidis.Results: The results showed that garlic extract (Allium sativum L.) had an antibacterial effect in inhibiting the growth of Staphylococcus epidermidis. The inhibitory zone concentration in the statistical results shows there are significant differences with values p = 0.000 or p <α.Conclusion: The conclusion of the study showed that Garlic Extract (Allium sativum L.) had an antibacterial effect to retarding the growth of Staphylococcus epidermidis..Keywords: Staphylococcus epidermidis, garlic, antibacteria

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Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

Hang Tuah Medical journal ◽

10.30649/htmj.v18i1.239 ◽

2020 ◽

Vol 18 (1) ◽

pp. 47

Author(s):

FERIZAL NEGERI SAMUDRA ◽

RETNO BUDIARTI ◽

IRMAWATI IRMAWATI

Keyword(s):

Sea Cucumber ◽

Antibacterial Effect ◽

Positive Control ◽

Negative Control ◽

Shigella Dysenteriae ◽

Control Group ◽

Control Groups ◽

Shigella Spp ◽

Diarrhea Disease

ABSTRACTBackground; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on Shigella Dysentriae growth in vitro is needed.Objectives: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the Shigella dysentriae bacteria in vitro.Method: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.Result: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.Conclusion: Sea cucumber (Holoturia scabra) inhibit the growth of Shigella dysenteriae.Key words: Shigella dysenteriae, sea cucumber (Holoturia scabra), antibacterial

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Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v5i1.6 ◽

2018 ◽

Vol 5 (1) ◽

pp. 58

Author(s):

Noer Kumala Indahsari ◽

Masfufatun Masfufatun ◽

Emilia Devi D.R

Keyword(s):

Treatment Group ◽

Moringa Oleifera ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Control Group ◽

High Dose ◽

Control Group Design ◽

Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

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Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽

10.35790/eg.v9i2.34982 ◽

2021 ◽

Vol 9 (2) ◽

pp. 238

Author(s):

Stevia E. Nonutu ◽

Damajanty H. C. Pangemanan ◽

Christy N. Mintjelungan

Keyword(s):

Side Effects ◽

Treatment Options ◽

Antibacterial Properties ◽

Fusobacterium Nucleatum ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

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Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

Journal of Islamic Medicine ◽

10.18860/jim.v1i2.4456 ◽

2017 ◽

Vol 1 (2) ◽

pp. 76-87

Author(s):

Muhammad Nur

Keyword(s):

Treatment Group ◽

Total Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

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