scholarly journals The study of the antagonistic and growth-stimulating characteristics of Bacillus subtilis strains, promising for the development of the effective biofungicides

2020 ◽  
Vol 21 (3) ◽  
pp. 263-272
Author(s):  
A. M. Asaturova ◽  
T. M. Sidorova ◽  
N. S. Tomashevich ◽  
N. A. Zhevnova ◽  
A. I. Khomyak ◽  
...  

In the period from 2012 to 2015 on Kalym winter wheat variety plants there were carried out field tests of the liquid culture of B. subtilis BZR 336g and BZR 517 strains isolated from natural sources that exhibit antifungal activity against phytopathogenic fungi Fusarium graminearum, F. culmorum, Microdochium nivale and Pyrenophora tritici-repentis, and are also able to have a positive effect on the growth and development of plants. Pre-sowing seed treatment and two preventive treatments during the growing season of the plants were carried out with the liquid culture of the strains. Comparison variants - treatment with water (control), with chemical (Raxil, KS; Alto Super, KS) and with biological (Fitosporin-M, L) standards. Biological efficacy against the complex of fusarium root rots was noted in the shooting stage of winter wheat and during ripening, and in some years reached 22.8 % for B. subtilis BZR 336g strain and 17.2 % for B. subtilis BZR 517 strain. In variants with presowing seed treatment and treatment of vegetative winter wheat plants with B. subtilis BZR 336g strain for three years of field trials the yield was from 5.0 to 7.4 t / ha, and when treated with B. subtilis BZR 517 strain - from 4.2 to 7.6 t / ha. The maximum additional yield was obtained in 2013 - a reliable yield increase was 1.6 t / ha for B. subtilis BZR 336g strain and 0.8 t / ha for B. subtilis BZR 517 strain. The ability of liquid cultures of the studied strains to protect winter wheat against harmful diseases at the same level and above the biological and chemical standards will help to expand the range of microbial biological products in the segment of the Russian market of environmentally friendly plant protection products.

2020 ◽  
Vol 203 ◽  
pp. 02012
Author(s):  
Irina Shabanova ◽  
Nikolay Neshchadim ◽  
Ksenia Gorpinchenko ◽  
Aleksandr Boyko

The influence of fertilizers and plant protection products for the mycotoxins, pesticides and heavy metals accumulation in the grain of winter wheat variety Antonina grown at the experimental station of the Kuban University was investigated. In the resulted grain there were no traces of the fungicide “Фалькон”, insecticide “Эйфория “ and herbicide “Секатор турбо” used in the cultivation. The content of T-2 toxin is almost at the level of MPC 0.1 mg / kg in all variants of the experiment that indicates the fungal infection invading from the soil. The content of Mn, Cu, Zn, Pb, Cd, Co, Ni, Cr in grain did not exceed MAC for adult nutrition.


2021 ◽  
pp. 20-24
Author(s):  
Artem Vladimirovich Ermilov ◽  
Roman Aleksandrovich Kamenev ◽  
Anatoly Petrovich Solodovnikov ◽  
Vladimir Nikolaevich Maksimchuk

The article presents an analysis of the determination of the effectiveness of mineral and organic fertilizers on the yield of winter wheat grain. The studies were carried out in 2017–2020 in the Rostov region on the southern chernozem. The object of research was the Doneko winter wheat variety. The predecessor is corn for grain. Ammonium nitrate, ANP fertilizer (16-16-16) and urea were used as mineral fertilizers, which were applied in the fertilization system of winter wheat and acted as a background option in the experimental scheme. ANP fertilizer (16-16-16) was introduced when sowing winter wheat, ammonium nitrate - scattered over thawed-frozen soil and carbamide in the heading phase by foliar method. Organomineral fertilizers were represented by the following types: Ruther, Leili 2000, Siamino Pro, Gumiful Pro, Soft Guard and Double Wine MKR (monocaliphosphate) produced by Biokepharm (Switzerland). The raw material for the production of organomineral fertilizers was the algae of the warm seas Laminaria. In the field experiment, the options for the joint application of foliar application and pre-sowing seed treatment with organomineral fertilizers were also studied. The control was the option without mineral fertilizers and the option with the background of the use of mineral fertilizers (farm fertilization system). It was found out that the use of organic fertilizers Ruter (0.5 or 0.25 l / t) and Leyli (0.25 l / t) for seed treatment before sowing, the use of Soft Guard (0.2 l / ha) and Gumiful Pro ( 0.2 l / ha) in the spring tillering phase and in the flag leaf phase against the background of sowing nitroammophoska at a dose of N32P32K32, random fertilizing on thawed-frozen soil with ammonium nitrate at a dose of N40 and the use of carbamide in the heading phase at a dose of N20 increased grain yield on average for 2018–2020 compared with the control (fertilization system of the farm) by 0.73 t / ha, or 23.0%. The maximum yield increase from the root-forming agent Ruther, used for pre-sowing seed treatment, reached 0.36 t / g, or 11.3%.  


2021 ◽  
Vol 51 (1) ◽  
pp. 18-24
Author(s):  
N. G. Vlasenko ◽  
S. V. Burlakova

The results of assessing the effect of biological preparations and a seed disinfectant on the technological qualities of grain of soft spring wheat Novosibirskaya 31 are presented. The effectiveness of the use of biological plant protection products was studied in the field experiment, laid down in 2020 in the forest-steppe conditions of the Ob region. Pre-sowing seed treatment included the following options: control (without treatment); Trichodermin, P (Trichoderma viride, titer more than 6 billion spores/g), consumption rate – 15 kg/t seed; Sporobacterin, SP (Bacillus subtilis + Trichoderma viride, strain 4097), consumption rate – 0.5 kg/ton of seeds; Scarlet, ME, chemical standard (imazalil (100 g/l) + tebuconazole (60 g/l), consumption rate – 0.3 l/t of seeds. The use of the preparations contributed to an increase in yield by 0.40 and 0.52 t/ha when using Trichodermin and Sporobacterin, respectively, and by 0.08 t/ha when using fungicide Scarlet. In this case, the mass of 1000 grains increased by 0.84, 0.80 and 0.96 g, respectively, relative to the control. The preparations Trichodermin and Sporobacterin had a significant effect on the growth of grain in length and width relative to the control – by 5.4-6.9 and 9.6%, Scarlet – by 10.6 and 13.9%, respectively. Pre-sowing seed treatment contributed to the growth of such indicators of the caryopsis as volume (by 19.6–29.3%), surface area (by 12.1–19.2%), and sphericity (by 6.3–7.8%). To a greater extent, they increased with the use of fungicide Scarlet. Getting larger grain led to an increase in the endosperm content by 0.76–1.14%. A close correlation has been shown between the indicators of the mass of 1000 grains and the linear grain sizes (r = 0.92–0.98), as well as with the grain volume, sphericity and endosperm content (r = 0.98–0.99). Pre-sowing treatment of spring wheat seeds provides grain with improved technological properties.


2016 ◽  
Vol 3 (0) ◽  
Author(s):  
Д. Ю. Дубовик ◽  
А. А. Сіроштан ◽  
Л. І. Ільченко ◽  
Б. А. Олефіренко

2021 ◽  
Author(s):  
Anna Wernecke ◽  
Jakob H. Eckert ◽  
Rolf Forster ◽  
Nils Kurlemann ◽  
Richard Odemer

Abstract Currently, more than 350 spray adjuvants are registered in Germany (January 2021). Unlike plant protection products (PPPs), adjuvants are not subjected to regulatory risk assessment. In practice, numerous combinations of PPPs and adjuvants are therefore possible. Thus, tank mixtures containing insecticides that are classified as nonhazardous to bees and approved for use in bee attractive crops may raise pollinator safety concerns when mixed with efficacy increasing adjuvants. This study analyzes whether selected “PPP-adjuvant” combinations result in increased mortality and pose an elevated risk to honey bees. To answer this question, we chose six common spray adjuvants of different classes for laboratory screening. These were then tested in a total of 30 tank mixtures, each with a neonicotinoid (acetamiprid), pyrethroid (lambda-cyhalothrin), diamide (chlorantraniliprole), carbamate (pirimicarb), and butenolide (flupyradifurone) formulation. We followed OECD test guideline 214 (acute contact test) but adopted the use of a professional spray chamber for more realistic exposures. Our results showed that, in total, 50% of all combinations significantly reduced the lifespan of caged honey bees in comparison to individual application of insecticides. In contrast, none of the adjuvants alone affected bee mortality (Cox proportional hazard model, p > 0.05). With four of the five insecticide formulations, the organosilicone surfactant Break-Thru® S 301 significantly increased bee mortality within 72 h (for all insecticides except chlorantraniliprole). Furthermore, acetamiprid yielded the highest and second-highest mortality increases from a tank mixture with the crop oil surfactants LI-700 (hazard ratio = 28.84, p < 0.05) and Break-Thru® S 301 (hazard ratio = 14.66, p < 0.05), respectively. In the next step, field trials should be performed to provide a more realistic exposure scenario under colony conditions to verify these findings.


Author(s):  
М. N. Zakharova ◽  
L. V. Rozhkova

In the Ryazan region using of modern and promising herbicides is effective in order to reduce the number and harmfulness of weeds. Initially, it is necessary to study the phytosanitary state of crops for each specific site and choose the most effective herbicide depending on the weeds species composition and thresholds of economic harm. The purpose of the study is to determine the biological and economic efficiency of drugs (Fenizan, BP and Morion, SK) and to assess their impact on the phytosanitary state of crops. The research was carried out on the experimental fields of the Institute of Seed Production and Agricultural Technology – it is a branch of the Federal Scientific Agroengineering Center VIM. The soil is dark gray forest heavy loamy, humus content – 4.0%, potassium and phosphorus – high, pH – 5.8. The cultivated plot area is 50 m2, four times the replication, the winter wheat variety – Viola. The predecessor is autumn fallow. The Azophoska (nitrogen-phosphorus-potassium fertilizer) application is 1.0 c / ha for pre-sowing cultivation. In the phase of spring tillering was performed fertilizing with ammonium nitrate (1.0 c / ha). The results of two-year field tests of post-emergence herbicides used in the autumn tillering phase to reduce weediness of winter wheat crop and increase yields are presented. It was found that in the region conditions of the tested preparations effectively reduced the contamination of crops and increased the grain yield of winter wheat by 2.5-5.5 c / ha.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Andrey Smagin ◽  
Nadezhda Sadovnikova ◽  
Marina Smagina

AbstractAnti-pathogenic protection of potatoes remains one of the most pressing problems of sustainable agronomy and plant protection. For this purpose, we propose to use a new type of synthetic hydrogels filled with amphiphilic recipients (dispersed peat, humates) and modern plant protection products. We assumed that the introduction of swollen gel structures into the rhizosphere of potatoes will allow us: to optimize the water supply and productivity of potatoes; to protect the fertile layer and potato tubers from the main pathogens; to fix modern plant protection products in the rhizosphere, keeping them from leaching and entering the environment. Preliminary laboratory experiments tested the anti-microbial activity of gel structures, as well as their water retention, dispersity and hydraulic conductivity with subsequent computer modeling of the water exchange and root uptake in the system of “soil-gel-potato”. Field trials were carried out in humid (European Russia) and arid (Uzbekistan) conditions under the atmospheric precipitation and irrigation on different soils and potato varieties with instrumental monitoring of environment, potato growth and quality. All experimental results confirmed the high efficiency of water-accumulative and plant protective synthetic gel structures. Their usage sufficiently (up to 6–15 t/hct) increases the potato yield with 1.3–2 times water saving, complete retention of agrochemicals in the rizosphere, and its actually total protection against major potato pathogens, including late blight (Phytophthora infestans).


2017 ◽  
Vol 8 (2) ◽  
pp. 848-853 ◽  
Author(s):  
A. Linz ◽  
D. Brunner ◽  
J. Fehrmann ◽  
T. Herlitzius ◽  
R. Keicher ◽  
...  

Precise applying of PPP (Plant Protection Products) in orchards and vineyards requires new kinds of sprayer technologies and new methods of sensor data evaluation. In this paper a selective electrical driven sprayer, carried by the autonomous robotic platform elWObot, is introduced. A 3D-Simulation environment and the framework ROS (Robot Operating System) helps developing and testing the interaction between the sprayer and the robot. The calculated leaf wall area (LWA) and the distance from the sprayer to the leaves in the spray region, control the flow-rate and the air-assist of eight adjustable sprayers individually. First field trials showed that the adaption of the software from the simulation to the hardware worked as expected.


2020 ◽  
Vol 33 (1) ◽  
pp. 43-53
Author(s):  
HENRIQUE MONTEIRO FERRO ◽  
RICARDO MAGELA DE SOUZA ◽  
FLAVIA MARA VIEIRA LELIS ◽  
JULIO CARLOS PEREIRA DA SILVA ◽  
FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS

ABSTRACT Ramulosis (Colletotrichum gossypii var. cephalosporioides) is an important fungal disease of cotton in Brazil, exclusively controlled by fungicide application. Therefore, sustainable management of ramulosis is essential. This work aimed to evaluate the potential of three bacterial strains, Bacillus amyloliquefaciens (UFLA285), Bacillus velezensis (UFLA401), and Paenibacillus lentimorbus (MEN2), for the biocontrol of ramulosis in cotton and their effects on yield and fiber quality. Seed treatment (ST), foliar spray, and soil drenching application methods were used (separately or combined) under greenhouse and field conditions. Chemical treatments recommended against ramulosis and water were used as controls. Under greenhouse conditions all strains reduced the disease incidence. While B. velezensis UFLA401 and P. lentimorbus MEN2 reduced the incidence by 56.6% and 45.7%, respectively, independent of the application method, B. amyloliquefaciens UFLA285 reduced the disease by about 60% when applied as a foliar spray or ST + foliar spray. Two field trials were performed and all bacterial strains reduced ramulosis incidence. In the first year, B. velezensis UFLA401 sprayed on the plants reduced incidence by 22.3% and ST + two foliar sprays resulted in the best performance, decreasing ramulosis by 57%. In both seasons the yield increased by using either bacterial or chemical treatments compared to the water control. The combination B. velezensis UFLA401 and P. lentimorbus MEN2 sprays provided better fiber quality than chemical treatment. Therefore, Bacillus sp. (UFLA285 and UFLA401) and P. lentimorbus MEN2 are potential tools to reduce ramulosis, increase cotton yield and fiber quality.


2021 ◽  
Vol 6 (48) ◽  
pp. 20-20
Author(s):  
Artem Ermilov ◽  
◽  
Roman Kamenev ◽  
Vladimir Turchin ◽  
Vera Kamenevа

Field experiments were conducted in 2017-2020 on the southern black soil in the conditions of the Lower Don. The Doneko winter wheat variety was sown. The predecessor of wheat is corn for grain. Mineral fertilizers were represented by ammonium nitrate (34.4%), nitroammophosk (16-16-16) and carbamide (46%), which acted as a background option in the scheme of the ex-periment. Organomineral fertilizers were preceded by the following agrochemicals: Ruther, Leyli 2000, Alga 1000/Turbo, Siamino Pro, Gumiful Pro, Soft Garden (Master Green Mix) and Double Vine (Monocaliphosphate). In the experiment, options for the joint use of non-root application and pre-sowing seed treatment with organomineral fertilizers were also studied. It was found that the use of organomineral fertilizers Ruther (0.5 or 0.25 l/ton) and Leyli (0.25 l/ton) for seed treatment before sowing, the use of Soft Guard (0.2 l/ha) and Humiful Pro (0.2 l/ha) in spring tillering and in the flag leaf phase against the background of a starter treatment mineral fertilizer in the form of azofoska at a dose of N32P32K32, fertilizing on frozen-thawed soil with ammonium nitrate at a dose of N40 and non-root topdressing with carbamide in the phase, earing at a dose of N20 increased the increase in grain yield on average for 3 years compared to the control variant (farm fertilizer system) by 0.73 t/ha or by 23.0%. The greatest increase in yield from the Router root-forming agent used for seed treatment was 0.36 t/ha or 11.3%. Keywords: WINTER WHEAT, MINERAL FERTILIZERS, ORGANOMINERAL FERTILIZERS, YIELD


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