scholarly journals The Effect of Administering Extract of Java Plum Ethanol (Syzygium Cumini) to the Expression of the Molecular Adhesion 1 (Icam-1) on the Model of Huvecs Preeclampsia

2020 ◽  
Vol 4 (1) ◽  
pp. 175-180
Author(s):  
Siswi Wulandari ◽  
Rahma Kusuma Dewi ◽  
Supraptinigsih Supraptinigsih
Keyword(s):  
Statistical Analysis ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Syzygium Cumini ◽  
Meaningful Difference ◽  
Dosage Group ◽  
Control Groups ◽  
Molecular Adhesion ◽  
Regression Test

Introduction: Preeclampsia is one of the causes of maternal mortality. In preeclampsia, endothelial dysfunction triggers the emergence of inflammatory cytokines and increases the expression of the molecular adhesion of ICAM-1. Inflammation of the preeclampsia can be controlled with the administration of anthocyanins. Java plum (Syzygium Cumini) contains anthocyanins that serve as antioxidants and anti-inflammatory. Purpose: This research aims to prove the influence of the administration of java plum (Syzygium Cumini) extracts on the expression of ICAM-1 on the model of HUVECs preeclampsia. Method: The research is conducted experimentally. It is done within the glass in a laboratory with two control groups namely negative control, three positive control, and six experimental groups (100ppm dose, 200ppm and 400ppm, incubation 1 hour, 3 hours, and 24 hours). HUVECs preeclampsia as a model of HUVECs preeclampsia within the glass. The ICAM-1 expression is used in the immunohistochemistry. The statistical analysis uses Two Way Anova test and regression test. Result: at the time of incubation of 1 hour, 3 hours, and 24 hours, there is no meaningful difference in the expression ICAM-1 (p = 0.392 > ∝). At the dose ICAM-1 of java plum, there is a meaningful difference between the positive control group with the dosage group of (p = 0.000 < ∝). Conclusion: An expression of ICAM-1 increases in preeclampsia conditions, administering java plum at a dose of 100 ppm, 200 ppm, and 400 ppm may decrease the expression of ICAM-1 in preeclampsia condition.

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

scholarly journals In Ovo β-Alanin Solüsyonu Alan Yeni Çıkmış Etlik Piliç Civcivlerinde Kuluçka Randımanı, Bazı Kuluçka Parametreleri, Kalite Skoru ve Yaşama Gücü

2018 ◽  
Vol 6 (10) ◽  
pp. 1469
Author(s):  
Canan Kop Bozbay ◽  
Ahmet Akdağ ◽  
Helin Atan ◽  
Nuh Ocak
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Quality Score ◽  
Control Group ◽  
Control Groups ◽  
In Ovo ◽  
Broiler Breeder ◽  
Death Rates ◽  
Chick Quality

This study was conducted to evaluate the effects of In ovo injection of β-Alanine which is a modified form of the alanine amino acid on hatchability, hatching weight, chick quality score and survival in broiler breeder eggs. For this purpose, 120 fertile eggs from Ross 308 breeder (32-week-old) were allocated randomly to four groups with 3 replicates. Except the negative control group eggs (non injected; NC), to the amniotic fluid of the eggs in the other 3 groups were injected with 1 ml solutions 0.9% salt (Positive control, PK), 0.75% (0.75βA) or 1.5% (1.5βA) by using 19 mm and 27 gauge needle. Death rates at the different stages of embryonic development, hatching yield, weights and quality scores of chicks and mortalities were recorded at hatching day. Hatching weight of the chicks in the 0.75βA group was higher than other two control groups. Accessing feed, determined as an indicator of chick quality, was better in the 0.75βA group. Conversely, mobility score of chicks in the NC group was higher than other groups. Hatching yield, chick quality score and mortalities of the first week were not affected from In ovo βA injection. Results of this study demonstrated that In ovo βA injection can be used up to 1.5% without any deteriorations on weight, quality and mortalities of chicks but 0.75% βA injection induced better hatching characteristics.

scholarly journals Natural plant extracts and prebiotic compounds as alternatives to antibiotics in broiler chicken diets in a necrotic enteritis challenge model

2013 ◽  
Vol 53 (12) ◽  
pp. 1247 ◽  
Author(s):  
J. K. Vidanarachchi ◽  
L. L. Mikkelsen ◽  
C. C. Constantinoiu ◽  
M. Choct ◽  
P. A. Iji
Keyword(s):  
Broiler Chickens ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Control Group ◽  
Soluble Carbohydrate ◽  
Necrotic Enteritis ◽  
Control Groups

An experiment was conducted to determine the effects of two different water-soluble carbohydrate extracts (renga renga lily extract and Acacia extract), and two commercially available prebiotic compounds, Fibregum and Raftifeed-IPE, on the performance of broiler chickens subjected to a necrotic enteritis (NE) challenge model. These treatments were compared with negative control and a positive (Zn-bacitracin) control treatments. An overall 8.8% NE-related mortality was recorded, with mean jejunal and ileal lesion scores in dead birds ranging from 3.03 to 3.90 in all challenged groups except the positive control groups. NE-specific deaths or clinical abnormalities were not observed with unchallenged control and positive control groups. At 7 days post-challenge, the concentration of specific IgY antibodies against the α-toxin of Clostridium perfringens in the serum was lower (P < 0.05) in birds fed the positive control and Fibregum-supplemented diets than in the negative control group. However, birds fed Fibregum had increased (P < 0.05) IgM concentration compared with those fed Acacia extract and lily extract. The Fibregum-fed group also had higher (P < 0.05) IgA concentrations in serum than did the positive-control and lily extract-supplemented groups at 14 days but this effect did not persist to 21 days. The results from the present study demonstrated that supplementation with water-soluble carbohydrates from two plant sources was not effective in controlling NE. However, the prebiotic compound Fibregum was found to be having some immunomodulatory effects. Addition of Zn-bacitracin and monensin was highly effective in counteracting the negative effects of the disease challenge.

scholarly journals Tissue injuries of wistar rats treated with hydroalcoholic extract of Sonchus oleraceus L.

2011 ◽  
Vol 47 (3) ◽  
pp. 605-613 ◽  
Author(s):  
Franciele Carla Prichoa ◽  
Silvane Souza Roman ◽  
Vanusa Manfredini
Keyword(s):  
Wistar Rats ◽  
Skin Lesions ◽  
Positive Control ◽  
Negative Control ◽  
Hair Follicles ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Sonchus Oleraceus ◽  
Experimental Group

The use of plant species is emerging as an important alternative in the treatment of injuries. Therefore, the extract of Sonchus oleraceus 10% was employed in the repair of skin lesions. A total of 36 male Wistar rats were subjected to a punch injury and divided into three groups: a negative control, receiving no treatment, a positive control, treated with Dersani, and the experimental group treated with the extract. The injury was assessed macroscopically and microscopically. Morphometric data was collected at the 3rd, 5th and 7th postoperative day, and the experimental group showed greater changes in shrinkage of the lesion compared to control groups. On the 3rd postoperative day, the injury in the experimental group showed less necrotic tissue, lower slough and more granulation tissue in relation to the positive control group. On the 7th and 10th postoperative day, the injury in the experimental group showed lower slough compared to the positive control group. Microscopic analysis of lesions on the 5th postoperative day revealed increased fibroplasia in the experimental group compared to control groups, while on the 14th postoperative day less neovascularization was evident in the experimental group and increased formation of hair follicles in the negative control group. The extract of S. oleraceus provided tissue repair in accordance with normal physiological patterns thus confirming empirical evidence for its use.

scholarly journals Spectrophotometric assessment of tooth bleaching under orthodontic braquets bonded with different materials

2017 ◽  
Vol 46 (5) ◽  
pp. 267-272
Author(s):  
Camila Maria Lima de CASTRO ◽  
Higor Catta Preta BORGES ◽  
Karoline Cardoso Ferro BARROS ◽  
Natanael Barbosa dos SANTOS ◽  
Larissa Silveira de Mendonça FRAGOSO
Keyword(s):  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Tooth Bleaching ◽  
Control Group ◽  
Control Groups ◽  
Orthodontic Bracket ◽  
Tooth Color ◽  
Bleaching Action ◽  
At Home

Abstract Introduction patients have been submitted to tooth bleaching during orthodontic treatment for aesthetic purposes or to anticipate the replacement of restorations after completion of the treatment. Objective to evaluate the effectiveness of tooth bleaching under orthodontic brackets bonded with different materials. Material and method a hundred bovine enamel blocks were divided into two groups, at-home and in-office tooth bleaching. In-office bleaching was subdivided into five groups (n = 10): HP (control - without brackets); SA(t) - brackets bonded with Transbond XT, without bleaching; SA(fm) - brackets bonded with Orthodontic Fill Magic, without bleaching; HP(t) - brackets bonded with Transbond XT subjected to bleaching; and HP(fm) - brackets bonded with Orthodontic Fill Magic subjected to bleaching. At-home bleaching followed the same treatments, only replacing the bleaching agent. Spectrophotometric assessment was used for tooth color determination at three moments: 1) before staining (baseline); 2) after staining; 3) after bonding the brackets and bleaching procedures. Data were submitted to ANOVA and analyzed by Tukey's test (p < 0.05). Result For both types of bleaching, the control group had an effective bleaching action. Groups using Transbond XT presented greater bleaching potential among the groups with orthodontic accessory, but the bleaching action differed from the positive control. Groups using Orthodontic Fill Magic presented no bleaching action, resembling the negative control groups (artificial saliva). Conclusion the orthodontic bracket impaired the effectiveness of the at-home and in-office bleaching treatment, regardless of the resin used for bonding.

scholarly journals Evaluation of the effect of different concentrations of Arsenicum album 6cH on intoxicated rats

2021 ◽  
Vol 8 (28) ◽  
pp. 119-127
Author(s):  
Olney Leite Fontes ◽  
Fátima Cristiane Lopes Goularte Farhat ◽  
Amarilys Toledo Cesar ◽  
Marilisa Guimarães Lara ◽  
Maria Imaculada Lima Montebelo ◽  
...  
Keyword(s):  
Atomic Absorption Spectroscopy ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Before And After ◽  
Male Wistar Rats

Aims: Homeopaths diverge on the concept of dose, i.e. the amount of drug that a patient must take to alter his or her state of disease. In order to stimulate reflections on this concept, this study sought to evaluate in vivo the effect of different concentrations of Arsenicum album 6cH prepared according to homeopathic pharmacotechnics. Methods: male Wistar rats were intoxicated with arsenic and then treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1%, administered orally. The amount of arsenic retained in the animals’ organism and that eliminated by urine were measured through atomic absorption spectroscopy. Samples of urine were collected before and after intoxication and during treatment. The positive control group (intoxicated animals) and the negative control group (non-intoxicated animals) received only the vehicle used in the preparation of the medicine. Results: Groups treated with Arsenicum album 6cH and Arsenicum album 6cH diluted at 1% eliminated significant amounts of arsenic when compared to the control groups. The group treated with Arsenicum album 6cH eliminated significantly higher amounts of arsenic than the group treated with the diluted medicine at 1%. Conclusion: results suggest that Arsenicum album 6cH should not be diluted as not to compromise its effectiveness in the treatment of rats intoxicated with arsenic.

scholarly journals Chitosan nanoparticle vaccine loaded with crude extracellular proteins of C. perfringens and Salmonella flagella is partially protective against necrotic enteritis in broiler chickens

2020 ◽  
Author(s):  
Gabriel Akerele ◽  
Nour Ramadan ◽  
Muhammed Mortada ◽  
Revathi Shanmugasundaram ◽  
Sankar Renu ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Positive Control ◽  
Negative Control ◽  
Extracellular Proteins ◽  
Positive Control Group ◽  
Economic Losses ◽  
Control Group ◽  
Necrotic Enteritis ◽  
Mrna Levels ◽  
Control Groups

AbstractNecrotic enteritis (NE) causes significant economic losses and food shortages world-wide. There are currently no licensed commercial vaccines against NE in broilers. Chitosan nanoparticles were formulated with extracellular proteins of C. perfringens surface-tagged with Salmonella flagellar proteins. One-day-old male broiler chicks were completely randomized to 3 treatments: Non-vaccinated non-challenged as negative control, Vaccinated-challenged, and non-vaccinated challenge as positive control. On day of hatch, d7, and d14 post-hatch, vaccinated-challenged birds were orally gavage with 50μg vaccine in 0.5ml PBS while positive control birds were gavage with 0.5ml PBS only. Birds in the vaccinated-challenged and positive control groups were orally infected on d14 post-hatch, with 5,000 oocysts/bird of E. maxima, followed by log 8 CFU of a virulent strain of C. perfringens on d19, d20, and d21 post-hatch. From d14 to 21 and d14 to 28 post-hatches, mortality in the vaccinated-challenged group was higher than that in the positive control group, approaching statistical significance (p=0.07). On d21 post-hatch, the mean lesion score of 3 birds/cage in the vaccinated-challenged group was higher than the positive control group, approaching statistical significance (p = 0.05). From d 14 to 28 post-hatch, the feed intake was higher and feed conversion ratio lower in the vaccinated-challenged group compared to the positive control group (p<0.05). On d21 post-hatch, antigen specific recall proliferation in the vaccinated-challenged group was higher than that in the negative and positive control groups (p<0.05). On d21 post-hatch, cecal tonsils CD8+ T lymphocytes expression in the vaccinated-challenged group was similar to the negative control group (p>0.05) but higher than that in the positive control group (p<0.05). Finally, vaccination resulted in an increase in ileal mRNA levels of zonula occluding on d21 post-hatch. In conclusion, there were numerical but not statistically significant decrease in NE lesions and mortality in vaccinated and challenged broilers. Further studies are needed to improve the efficacy of the vaccine and understand the mechanism underlying protection in vaccinated birds.

scholarly journals The Role of Ceftazidime as a Photosensitizer in Human Erythrocytes Through Oxidative Stress Mechanism

2018 ◽  
Vol 10 (2) ◽  
pp. 128-32 ◽  
Author(s):  
Mashuri Mashuri ◽  
Mustofa Ruhullah ◽  
Bayu Diertama Putera ◽  
Vicky Pramudinta Mega ◽  
Fadillah Alma Putra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbonyl Compounds ◽  
Superoxide Radical ◽  
Uv Light ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups

BACKGROUND: Ceftazidime was known to cause photosensitization reactions. However, how it plays the role remained unclear. In our study, we aim to investigate the photosensitization effect of ceftazidime in erythrocytes via oxidative stress.METHODS: Samples were divided into six different groups: negative control group, positive control group and four experimental groups with 10%, 20%, 30%, and 40% ceftazidime, respectively. The positive control and experimental groups were exposed to ultraviolet (UV)-light for 2 hours. Superoxide radical, malondialdehyde (MDA), carbonyl compounds (CC) and methemoglobin (Met-Hb) levels were then measured.RESULTS: The results showed a significant increased of superoxide radical, MDA, CC and Met-Hb levels in all experimental groups compared to both negative or positive control groups (p< 0.05).CONCLUSION: In conclusion, our study confirmed the role of ceftazidime as a photosensitizer in erythrocytes via the oxidative stress mechanisms.KEYWORDS: ceftazidime, oxidative stress, photosensitizer, photosensitization.

scholarly journals The effectiveness of Boiled figs leaf (FIcus Carica L) and Rosy Periwinkle (Catharantus roseus) on SGOT and SGPT levels of male wistar strain rats with hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 850-859
Author(s):  
Debora Oktaviani
Keyword(s):  
Treatment Group ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Wistar Strain ◽  
Significant Difference

Hepatitis is an inflammatory disease of liver cells that is contagious in the low category, the cause of it is bacteria, viruses, drugs and alcohol. The purpose of this study was to determine the effectiveness of boiled figs leaf (Ficus Carica L) and Rosy periwinkle (Catharantus roseus ) in reducing SGOT and SGPT levels. The subjects of this study were 30 male Wistar strain rats with age of 2-3 months, body weight 180-200 grams. Rats were divided into 3 groups; treatment groups, negative control groups and positive control groups. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. The treatment group was given 0.4 grams boiled figs leaf (Ficus Carica L) and 2.6 grams boiled rosy periwinkle  (Catharantus roseus) it given as much as 3.6 cc / day orally for 7 days. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare SGOT and SGPT levels. The results showed there were significant differences in SGOT levels between the treatment group, the positive control group, and the negative control group (p <0.05) and there was a significant difference in the SGPT value between the treatment group and the positive control group (p <0.05), while the negative control group and the treatment group there was no significant difference (p = 0.75). The conclusion of this study was the water extraction of figs leaf (Ficus Carica L), Rosy periwinkle (Catharantus roseus) had an effect in decreasing the SGOT and SGPT values of male wistar strain rats induced by paracetamol.

scholarly journals Effect of selenium applied to pasture on the selenium status of grazing sheep

1999 ◽  
pp. 69-73
Author(s):  
A.M. Moorhouse ◽  
C.T. Westwood ◽  
A.J. Dumbleton ◽  
L.P. Donnelly ◽  
L.A. Bridger
Keyword(s):  
Slow Release ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Groups ◽  
Selenium Treatment ◽  
Second Year ◽  
Highly Correlated ◽  
Selenium Supplements

Blood selenium concentrations and liveweight gains were assessed for sheep grazing pastures treated with different selenium supplements over a 2-year period. Three 0.5 hectare irrigated blocks with pasture selenium concentrations of less than 0.04 μg/g DM were identified near Lincoln, Canterbury during 1997. Treatments applied in early November 1997 were: (1) 1 kg/ha of an experimental selenium product (SRSe5, containing 10g selenium); (2) 1 kg/ha of a commercially available slow release selenium product (positive control, containing 10g selenium) and (3) nil selenium treatment (negative control); all treatments used 60 kg urea/ha as the carrier. Treatments were stocked at 30 lambs/ha in November 1997. Liveweight gains and concentrations of blood selenium were monitored fortnightly for the first 4 months of the study and monthly thereafter. Following mating in April 1998, blood selenium concentrations and liveweight gains of spring born progeny were assessed. Ewes grazing the SRSe5 and positive control treatments had significantly greater concentrations of blood selenium than the negative control group and the concentrations for the SRSe5 group were significantly greater than from the positive control. Negative control ewes and their lambs were removed from the study at lambing due to low blood selenium concentrations and concerns regarding their health. Concentrations of blood selenium did not differ significantly between lambs of SRSe5 and positive control ewes born in the second year of the study. Lamb and ewe blood selenium concentrations were highly correlated. Liveweight gains did not differ significantly between SRSe5, positive control or negative control groups for ewes or lambs during either year of the study. This study demonstrated that the application of selenium to pastures is a highly effective longterm strategy by which concentrations of blood selenium may be elevated in sheep under New Zealand pastoral conditions. While liveweights did not differ significantly between treatments during either year of the study, the small numbers of animals used limited the power with which differences could be detected between treatments. Keywords: pasture, selenium, sheep, supplementation, topdressing

Sign in / Sign up

Export Citation Format

Share Document