scholarly journals Toxicological Evaluation of the Complex Insectoacaricidal Preparation "Neoterika Protecto 12"

2018 ◽  
Vol 12 (3) ◽  
pp. 60-66
Author(s):  
E.N. Indyuhova ◽  
M.V. Arisov ◽  
G.B. Arisova ◽  
I.A. Stepanova
Keyword(s):  
Aqueous Suspension ◽  
Hazardous Substances ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Toxicological Evaluation ◽  
Cutaneous Toxicity ◽  
Oral Supplementation ◽  
Subacute Toxicity ◽  
White Rats ◽  
Active Substances

The purpose of the research: toxicological evaluation of a medicinal product for veterinary use "Neoterica Protecto 12" on laboratory animals. Materials and methods. White outbred rats, mice and guinea pigs were used in the experiment. In the study of acute oral toxicity in mice and rats, acute cutaneous toxicity, subacute toxicity, irritant action of an aqueous suspension of a combination of active substances, allergenic properties of the collar «Neoterika Protecto 12» conventional methods were used. Results and discussion. The median lethal doses LD50 for oral supplementation to white mice (1070 mg/kg) and oral supplementation to white rats (3210 mg/kg) were diagnosed, which allowed to designate the preparation to the 3rd hazard class (moderately hazardous substances). In the study of acute cutaneous toxicity on white rats LD50 amounted to more than 10 000 mg/kg, therefore, according to the generally accepted hygienic classification, the preparation belongs to the 4th hazard class during cutaneous application. In the study of subacute toxicity of the medication on rats for 6 months after application of the aqueous suspension of the combination of active ingredients in doses of 1000, 500 and 200 mg/kg, no changes in general state and behavior were observed in animals. When an aqueous suspension of a combination of active substances of the collar was applied to the skin of rats, there were no signs of irritant effect, but a mild effect on the mucous membrane of the guinea pig's eyes was recorded. It was found that the preparation does not have allergic and sensitizing effects.

scholarly journals TOXICOLOGICAL CHARACTERISTICS OF A VETERINARY ANTI-PARASITIC PREPARATION WITH A PROLONGED ACTION IN THE FORM OF A SOLUTION FOR INTERNAL ADMISSION

2021 ◽  
pp. 67-73
Author(s):  
Arisova
Keyword(s):  
Oral Solution ◽  
Female Rats ◽  
Hazardous Substances ◽  
Male Rats ◽  
Prolonged Action ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Hazard Class ◽  
Cutaneous Toxicity ◽  
White Rats

This article presents the results of studies of the toxicological characteristics of a longacting veterinary medicinal product "Neoterica Protecto Syrup" based on moxidectin in the form of an oral solution. Acute oral toxicity was studied in white mice and white rats. As a result, the LD50 of the drug was 4300 mg/kg for male and female mice, 3500 mg/kg for male rats, and 5700 mg/kg for female rats, and on the basis of which it was established that the drug belongs to the 3rd hazard class (moderately hazardous substances) according to GOST 12.1.007-76. Acute cutaneous toxicity was investigated and the irritant effect of the drug on white rats was assessed, for the drug the 4th hazard class (low-hazard substances) was established according to GOST 12.1.007-76. The subchronic toxicity of doses of 1/10, 1/20 and 1/50 of the LD50 of the drug, established in an acute experiment, with daily use for 5 days was studied in white rats. As a result, it was found that doses of 350, 175 and 70 mg/kg with daily use in white outbred rats for 5 days are inoperative.

TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

1970 ◽  
pp. 67-69
Author(s):  
E. K. Rakhmatullin ◽  
O. D. Sklyarov
Keyword(s):  
Drug Toxicity ◽  
Probit Analysis ◽  
Laboratory Animals ◽  
Control Group ◽  
Hazard Class ◽  
Water Ingestion ◽  
White Rats ◽  
Poorly Soluble ◽  
And Behavior ◽  
Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

scholarly journals Preclinical evaluation of the veterinary drug “Amoxidev 15” for its use in surgical and obstetric practice

2021 ◽  
Vol 23 (103) ◽  
pp. 109-115
Author(s):  
L.-M. Kostyshyn ◽  
R. Sachuk ◽  
Ye. Kostyshyn ◽  
O. Katsaraba
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Control Level ◽  
Subcutaneous Administration ◽  
The Body ◽  
Laboratory Animals ◽  
Veterinary Drug ◽  
Intragastric Administration ◽  
Toxicological Evaluation ◽  
White Rats

Suspension for injection “Amoxidev 15” is prescribed to fur-bearing animals (mink, fox), dogs and cats for the treatment of respiratory diseases (tonsillitis, tracheitis, pneumonia, bronchitis, rhinitis, sinusitis, bronchopneumonia), digestive (gastritis, enteritis, enteritis). genitourinary systems (nephritis, urethritis, urocystitis, mastitis, metritis, agalactia), musculoskeletal system (arthritis, osteoarthritis, joint injuries, tendonitis, hoof lesions), skin and soft tissues (eczema, dermatitis) caused by sensitive drug by microorganisms, including colibacillosis, streptococcus, bronchopneumonia, etc. Toxicological evaluation of the veterinary drug “Amoxidev 15” under the conditions of acute and subacute toxicological experiments on a model of white rats. According to the results of an acute toxicological experiment with intragastric administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration. The maximum administered dose (in absolute weight of the drug) was 20000.0 mg/kg body weight, which allows to refer the drug to class VI toxicity of relatively harmless substances (DL50 > 15000 mg/kg body weight), and the degree of safety to class IV – low-hazard substances (DL50 > 5000 mg/kg). According to the results of an acute toxicological experiment with subcutaneous administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration, the maximum dose was 5000.0 mg/kg body weight, therefore, the drug “Amoxidev 15” when administered subcutaneously by toxicity can be classified as class VI substances relatively harmless (DL50 Subcut > 4500.0 mg/kg). When administered subcutaneously to white rats, the drug “Amoxidev 15” under conditions of subacute toxicological experiment in doses of 0.1–1.0 ml/kg does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, although 3-day administration of the drug in a dose 1.0 ml/kg body weight caused an increase in the activity of hepatospecific enzymes ALT and AST by 12.5 and 11.1 % (P < 0.05), respectively, relative to the control, which was restored to the control level 7 days after cessation.

scholarly journals STUDY OF TOXICOLOGICAL PROPERTIES AND LOCAL IRRITANT EFFECT OF THE DRUGS INSACAR TOTAL C PLUS AND INSACAR TOTAL K PLUS ON LABORATORY RATS

2021 ◽  
pp. 330-335
Author(s):  
Makhvatova
Keyword(s):  
Parasite Species ◽  
Systemic Effect ◽  
Single Application ◽  
Active Components ◽  
Cutaneous Toxicity ◽  
Laboratory Rats ◽  
Entire Observation Period ◽  
White Rats ◽  
Active Substances ◽  
Observation Period

The study of acute cutaneous toxicity and local irritant effect of the drugs Insacar Total C Plus and Insacar Total K Plus on mongrel white rats was conducted in doses of 5000 and 10 000 mg/kg of body weight (BW). When the drugs were applied to the skin of rats, no animal deaths were recorded during the entire observation period. Thus, the LD50 is more than 10 000 mg / kg of BW. A single application of the drugs to the skin in rats at a dose of 1 ml/100 g (10 000 units) in two experimental groups on the 7th and 9th day of the study showed the most pronounced irritant effect. In this regard, the drugs have an irritating effect – 1 point. The studied drugs Insacar Total C Plus and Insacar Total K Plus belong to the group of insectoacaricidal and anthelmintic agents. The substances included in its composition have a contact and systemic effect on ectoparasites and nematodes. They are complex medicines containing 4 active components – moxidectin, pyriproxifen, imidacloprid, praziquantel. Such a combination of active substances is characterized by effectiveness against a larger number of parasite species, stages of their development, and in addition, reduce the likelihood of developing resistance of pathogens to drugs.

Toxicological characteristics of a medicinal product for veterinary «Ketoprofen 10%» in laboratory animals (acute toxicity)

2021 ◽  
Vol 1 (1) ◽  
pp. 29-40
Author(s):  
A. N. Shkatova ◽  
◽  
D. A. Devrishov ◽  
O. B. Litvinov ◽  
V. E. Brylina ◽  
...  
Keyword(s):  
Medicinal Product ◽  
Study Drug ◽  
Probit Analysis ◽  
Hazardous Substances ◽  
Laboratory Animals ◽  
Intragastric Administration ◽  
Species Sensitivity ◽  
Hazard Class ◽  
Single Intravenous Injection ◽  
Ld50 Value

In the course of the studies, the toxicological properties of the medicinal product for veterinary «Ketoprofen 10%» in laboratory animals were studied. With a single intragastric administration of the drug to rats, the LD50 value calculated by the Kerberian method was 333,3 mg/kg. At the same time, the LD50 value by the Miller and Tainter method was 358,1±159,8 (198,3 ÷ 518,0) mg/kg. After a single intravenous injection of the study drug to rats, the LD50 value calculated by the Kerberian method was 351,1 mg/kg. The LD50 calculated by probit analysis according to Miller and Tainter was 349,7±167,5 (182,3 ÷ 517,2) mg/kg. Taking into account the obtained LD50 value in rats with intragastric administration and according to the generally accepted hygienic classification, the drug belongs to the 3rd hazard class – moderately hazardous substances (GOST 12.1.007-76). Intragastric administration of the drug to mice made it possible to obtain an LD50 value of 6249,4 mg/kg (according to Kerber). The LD50 calculated by probit analysis according to Miller and Tainter was 7725,0±2646,5 (5078,5 ÷ 10) mg/kg. Taking into account the data obtained on mice and according to the generally accepted hygienic classification, the drug belongs to the 4th hazard class – low-hazard substances (GOST 12.1.007-76). The coefficient of species sensitivity was more than 17, which indicates a pronounced species resistance to ketoprofen in mice compared to rats.

STUDY OF THE ACUTE TOXICITY OF AQUADES-NUK PRODUCTS 5

2021 ◽  
Author(s):  
Oleg P. Pugach ◽  
Alexander M. Lunegov ◽  
Irina V. Lunegova
Keyword(s):  
Acute Toxicity ◽  
Animal Feed ◽  
Raw Materials ◽  
Animal Husbandry ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Source Of Infection ◽  
White Rats ◽  
Quality Of Products ◽  
Susceptible Organism

In the system of veterinary and sanitary measures, disinfection occupies one of the important places, contributing to ensuring the welfare of animal husbandry against infectious diseases, increasing the productivity of animals, poultry and the sanitary quality of products, raw materials and animal feed. The main purpose of disinfection is to break the epizootic chain by influencing its most important link - the factor of transmission of the causative agent of the disease from the source of infection to the susceptible organism. Based on the foregoing, we were assigned the goal of studying the characteristics of the new domestic disinfectant AQUAdez-NUK 5, and to achieve this goal, one of the tasks was to study acute toxicity. In order to study the acute toxicity of AQUAdes-NUK 5 when administered orally, experiments were carried out on male Wister white rats weighing 220-230 g. at doses of 2000, 3000, 4000 and 5500 mg / kg. For the experiments, the rats were divided into groups of 10 animals. 10 animals were used to study each dosage for each disinfectant. One group of ten animals served as a control, which was injected with 0.9% sodium chloride solution. The disinfectant was injected in pure form. After administration, the laboratory animals were monitored for two weeks. The experiment took into account the death of animals and the clinical picture of intoxication. In the course of laboratory studies, we determined that AQUAdez-NUK 5 belongs to the III hazard class in accordance with GOST 12.1.007-76 (moderately hazardous compounds), as well as the presence of a weak irritant effect, and therefore we took appropriate safety measures during work with this disinfectant.

scholarly journals Determination of toxicity indicators and assessment of the sensibilizing action of the preparation for the external use ‘Ointment for wounds’

2019 ◽  
Vol 5 (3) ◽  
pp. 22-26
Author(s):  
R. M. Sachuk
Keyword(s):  
Essential Oils ◽  
Mucous Membrane ◽  
Repeated Administration ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Tea Tree ◽  
Subacute Toxicity ◽  
Buccal Mucous Membrane ◽  
Sensitizing Effect

The purpose of the work is a determination of toxicity and sensitizing effects of a new external preparation ‘Ointment for wounds’, based on the essential oils of Siberian pine, eucalypt, tea-tree, cedar, clove, and oil solution of chlorophyllite. The experimental study was performed on rats weighing 160–190 g, 2–3 months of age and mice weighing 18–21 g, 3 months of age. At the stages of preclinical study, it was determined acute and subacute effects of the drug, when administrated on the skin and directly into the stomach of experimental animals. The skin-irritant effect of the phytopreparation was investigated when applied to the skin and buccal mucous membrane. The sensitizing effect of the ointment was evaluated by reproducing local reactions. The acute toxicity assessment was performed on the survival rate of laboratory animals after oral administration of the preparation at doses from 5,000 to 25,000 mg/kg of body weight, with 5,000 units increments. Subacute toxicity in the experiment was evaluated by the dynamics of morphological and biochemical parameters of blood and the study of the coefficients of laboratory animals’ internal organs mass. It was found that the introduction of the drug ‘Ointment for wounds’ in the stomach did not cause significant changes in the behavior of rats, all animals remained alive. Studies have shown the absence of skin-irritant effect of the preparation, as well as the absence of irritant effect on the buccal mucous membrane. Even in a long-term experiment to study subacute toxicity when applied to the skin, no toxic effects of the preparation on the basis of essential oils and oil solution chlorophyllite of were found. The index sensitizing effect of the preparation was less than one, indicating the absence of sensitizing effect. According to the classification of substances by toxicity and danger (requirements of SOU 85.2-37-736:2011 and GOST 12.1.007-76) belong to hazard class IV. In general, ‘Ointment for wounds’ does not have a toxic effect on the functions of vital organs, and at repeated administration is almost harmless

scholarly journals TOXICOLOGICAL CHARACTERISTICS OF MODERN PESTICIDES USED IN THE SYSTEM OF STRAWBERRIES CHEMICAL PROTECTION. REVIEW

2017 ◽  
Vol 13 (1-2) ◽  
pp. 106-112
Author(s):  
T.I. Zinchenko
Keyword(s):  
First Integral ◽  
Inhalation Toxicity ◽  
Active Ingredients ◽  
Hazard Class ◽  
Toxicological Evaluation ◽  
Toxicological Assessment ◽  
Chemical Protection ◽  
Active Substances

Relevance. The strawberries are consumed mostly fresh, that is why the toxicological assessment of chemical protection formulations applied on strawberries is compulsory before its entering the market. Objective: toxicological characteristics of modern pesticides used in the system of strawberries chemical protection. Materials and methods. Aktara 25 WG, inc., Switch 62.5 WG, Force 1.5 G, G, Nuprid 600 TN, and Torero SC pesticide preparations containing thiamethoxam, cyprodinil, fludioxonil, tefluthrin, imidacloprid, methamitron, and ethofumezate were the materials of the toxicological evaluation conducted according to the data of literary and official Internet sources. Results. A analysis of the literature on the toxic properties of the studied pesticides and its active ingredients used to protect strawberries has been carried out and the classes of hazard were established according to the current Ukrainian hygienic classification of pesticides. Conclusion. Switch 62.5 WG, Force 1.5 G, G, Nuprid 600 TN, and Torero SC pesticide preparations and fludioxonil, cyprodinil and metamitron active ingredients are classified according to acute inhalation toxicity as limiting criterion to the second integral hazard class (moderately hazardous), and Aktara 25 WG, preparation and thiamethoxam – to third integral hazard class (hazardous), active substances imidacloprid, tefluthrin, ethofumezate – to the first integral hazard class (extremely hazardous).

scholarly journals TOXICOLOGICAL EVALUATION OF BENZOPHENONE AS A BASIS OF ITS HYGIENIC NORMALIZATION IN THE AIR OF THE WORKING ZONE

2018 ◽  
Vol 97 (7) ◽  
pp. 660-663
Author(s):  
Nina A. Martynova ◽  
V. V. Zakharenkov ◽  
A. M. Oleshchenko ◽  
L. G. Gorokhova
Keyword(s):  
Acute Poisoning ◽  
Hazardous Substances ◽  
Male Rats ◽  
Class Iii ◽  
Hazard Class ◽  
Toxicological Evaluation ◽  
Working Zone ◽  
Safe Level ◽  
And Gender ◽  
Mild Irritant

For the purpose of hygienic rationing of benzophenone in the air of the working zone its toxic properties have been studied. DL50 for male rats, male and female mice amounted to 3000, 2000 and 1300 mg/kg, respectively. Benzophenone refers to moderately hazardous substances (hazard class III). The clinical picture of the acute poisoning with this substance was characterized by the low mobility, myorelaxation, shaky gait, breathing loss, weakened response to external stimuli and death on days 1 to 3 after the poisoning. There were no significant differences in the species and gender susceptibility of animals to the substance: coefficients of species differences and gender susceptibility ≈1.5. Benzophenone has an average cumulative capacity: a cumulation factor accounts for 3.2. It does not have a local irritant effect on the skin and skin-resorptive effect. It has a mild irritant effect on the mucous membranes of the eyes. There was no sensitizing effect in benzophenone: the specific leukocyte lysis reaction, the specific agglomeration of leukocytes and the eosinophil content in the blood of the experimental guinea pigs did not differ significantly from the control. After single 4-hour inhalation primers of benzophenone at concentrations of 35.2, 94.6 and 200 mg/m3, there were no violations of the synthetic and enzymatic functions of the liver. The threshold of acute inhalation action is 94.6 mg/ m3 (for decreasing body temperature, increasing the summation threshold and leukocytosis). Approximate safe level of exposure to benzophenone in the air in the working area is 2 mg/m3 (aerosol).

scholarly journals STUDY OF PHARMACO-TOXICOLOGICAL PROPERTIES OF A QUATERNARY AMMONIUM COMPOUND THAT IS PART OF A DISINFECTANT

2021 ◽  
Vol 247 (3) ◽  
pp. 187-191
Author(s):  
F.M. Nekhaychik ◽  
◽  
Keyword(s):  
Quaternary Ammonium ◽  
Lethal Dose ◽  
Quaternary Ammonium Compound ◽  
Laboratory Animals ◽  
Ammonium Compound ◽  
Oral Toxicity ◽  
Hazard Class ◽  
White Rats ◽  
Medical University ◽  
And Control

The study of the toxicological properties of a new disinfecting quaternary ammonium compound was carried out on laboratory animals of the vivarium of the Kazan State Medical University. Toxicological properties were evaluated on white mice, white rats, guinea pigs and rabbits by forming experimental and control groups. The value of acute oral toxicity, the indicator of the average lethal dose was experimentally determined, allergic properties were determined and the local irritant effect was evaluated. During the tests, it was found that the new quaternary ammonium compound belongs to the third hazard class.

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