Differential toxicity of copper (II) oxide nanoparticles of similar hydrodynamic diameter on human differentiated intestinal Caco-2 cell monolayers is correlated in part to copper release and shape

2011 ◽  
Vol 6 (7) ◽  
pp. 789-803 ◽  
Author(s):  
Jean-Pascal Piret ◽  
Sébastien Vankoningsloo ◽  
Jorge Mejia ◽  
Florence Noël ◽  
Emmanuelle Boilan ◽  
...  
Keyword(s):  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Cell Monolayers ◽  
Copper Release ◽  
Differential Toxicity

Effect of low-dose exposure of aluminium oxide nanoparticles in Swiss albino mice: Histopathological changes and oxidative damage

2020 ◽  
Vol 36 (8) ◽  
pp. 567-579
Author(s):  
Arpita De ◽  
Swarupa Ghosh ◽  
Manoswini Chakrabarti ◽  
Ilika Ghosh ◽  
Ritesh Banerjee ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Damage ◽  
Redox Homeostasis ◽  
Aluminium Oxide ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Physico Chemical ◽  
Living Organisms ◽  
Aluminium Oxide Nanoparticles

Rapid growth in the use of aluminium oxide nanoparticles (Al2O3 NPs) in various fields such as medicine, pharmacy, cosmetic industries, and engineering creates concerns since the literature is replete with data regarding their toxicity in living organisms. The objective of the present study was to demonstrate the potential toxicological manifestations of repeated exposure to Al2O3 NP at low doses in vivo. In the present study, Al2O3 NP was orally administered at 15, 30 or 60 mg kg−1 body weight for 5 days to Swiss albino male mice. A battery of well-defined assays was undertaken to evaluate aluminium (Al) bioaccumulation, haematological and histological changes, oxidative damage and genotoxicity. Physico-chemical characterisation demonstrated increases in hydrodynamic diameter along the concentration gradient of Al2O3 NP dispersed in MilliQ water. Brain, liver, spleen, kidney and testes showed high Al retention levels. Histopathological lesions were prominent in the brain and liver. Al2O3 NP treatment increased levels of lipid peroxidation and decreased glutathione content in the test organs at all dose levels. The enzyme activities of catalase and superoxide dismutase were also significantly altered. DNA damage quantified using the comet assay was markedly increased in all the soft organs studied. Anatomical abnormalities, redox imbalance and DNA damage were positively correlated with Al retention in the respective organs. Size, zeta potential and colloidal state might have contributed to the bio-physico-chemical interactions of the NPs in vivo and were responsible for the non-linear dose response. The overall data indicate that Al2O3 NP exposure may result in adverse health consequences, inclusive of but not limited to disturbed redox homeostasis, hepatocellular toxicity, neurodegeneration and DNA damage.

ß-Amido Acid Functionalised Superparamagnetic Iron Oxide Nanoparticles as a Novel Carrier for Efficient Delivery of Doxorubicine

2017 ◽  
Vol 41 (3) ◽  
pp. 129-135
Author(s):  
Fatemeh Ebrahimi ◽  
Maryam Karimi ◽  
Hasan Sereshti ◽  
Mohsen Yousefifar
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Precipitation Method ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Amido Acid

Superparamagnetic iron oxide nanoparticles are increasingly used in medical applications due to their unique physical properties. They are useful carriers for delivering antitumour drugs in targeted cancer treatment. In this study, amido acid-functionalised magnetic nanoparticles (AAFMNs) are used as drug-delivery vehicles for doxorubicine as an efficient tool for the treatment of cancer. Magnetic iron oxide nanoparticles were synthesised using a co-precipitation method. The prepared iron oxide nanoparticles were then functionalised with amido acid functional groups. Finally, the synthesised AAFMNs were used for the delivery of doxorubicine. AAFMNs were characterised by Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis and zeta potential. An in vitro-determined hydrodynamic diameter of ∼80 nm suggested their applicability for this purpose. The findings show that AAFMNs are a promising tool for potential magnetic drug delivery.

scholarly journals Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1862 ◽  
Author(s):  
Julieta C. Imperiale ◽  
Inbar Schlachet ◽  
Marianela Lewicki ◽  
Alejandro Sosnik ◽  
Mirna M. Biglione
Keyword(s):  
Interferon Alpha ◽  
Viral Infections ◽  
Chitosan Nanoparticles ◽  
Area Under The Curve ◽  
Hydrodynamic Diameter ◽  
Cell Compatibility ◽  
Cell Monolayers ◽  
Pegylated Nanoparticles ◽  
Oral Pharmacokinetics ◽  
Poly Ethylene

Interferon alpha (IFNα) is a protein drug used to treat viral infections and cancer diseases. Due to its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability, which is associated with severe side effects. We hypothesized that the nanoencapsulation of IFNα within nanoparticles of the mucoadhesive polysaccharide chitosan would improve the oral bioavailability of this drug. In this work, we produced IFNα-loaded chitosan nanoparticles by the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index and concentration were characterized by dynamic light scattering and nanoparticle tracking analysis. After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability of unmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured in monoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated that the nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the study of the oral pharmacokinetics of nanoencapsulated IFNα in BalbC mice revealed two maxima and area-under-the-curve of 56.9 pg*h/mL.

scholarly journals Trastuzumab Modified Barium Ferrite Magnetic Nanoparticles Labeled with Radium-223: A New Potential Radiobioconjugate for Alpha Radioimmunotherapy

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2067
Author(s):  
Weronika Gawęda ◽  
Marek Pruszyński ◽  
Edyta Cędrowska ◽  
Magdalena Rodak ◽  
Agnieszka Majkowska-Pilip ◽  
...  
Keyword(s):  
Barium Ferrite ◽  
Magnetic Field Gradient ◽  
Hydrodynamic Diameter ◽  
Nuclear Space ◽  
Her2 Receptor ◽  
Ovarian Adenocarcinoma ◽  
Cell Monolayers ◽  
Cell Internalization ◽  
Radium 223 ◽  
High Cytotoxicity

Barium ferrite nanoparticles (BaFeNPs) were investigated as vehicles for 223Ra radionuclide in targeted α-therapy. BaFe nanoparticles were labeled using a hydrothermal Ba2+ cations replacement by 223Ra with yield reaching 61.3 ± 1.8%. Radiolabeled nanoparticles were functionalized with 3-phosphonopropionic acid (CEPA) linker followed by covalent conjugation to trastuzumab (Herceptin®). Thermogravimetric analysis and radiometric method with the use of [131I]-labeled trastuzumab revealed that on average 19–21 molecules of trastuzumab are attached to the surface of one BaFe–CEPA nanoparticle. The hydrodynamic diameter of BaFe–CEPA–trastuzumab conjugate is 99.9 ± 3.0 nm in water and increases to 218.3 ± 3.7 nm in PBS buffer, and the zeta potential varies from +27.2 ± 0.7 mV in water to −8.8 ± 0.7 in PBS buffer. The [223Ra]BaFe–CEPA–trastuzumab radiobioconjugate almost quantitatively retained 223Ra (>98%) and about 96% of 211Bi and 94% of 211Pb over 30 days. The obtained radiobioconjugate exhibited high affinity, cell internalization and cytotoxicity towards the human ovarian adenocarcinoma SKOV-3 cells overexpressing HER2 receptor. Confocal studies indicated that [223Ra]BaFe–CEPA–trastuzumab was located in peri-nuclear space. High cytotoxicity of the [223Ra]BaFe–CEPA–trastuzumab bioconjugate was confirmed by radiotoxicity studies on SKOV-3 cell monolayers and 3D-spheroids. In addition, the magnetic properties of the radiobioconjugate should allow for its use in guide drug delivery driven by magnetic field gradient.

Synthesis and Phase Transfer of Monodisperse Iron Oxide (Fe3O4) Nanocubes

2014 ◽  
Vol 67 (4) ◽  
pp. 663 ◽  
Author(s):  
Melissa R. Dewi ◽  
William M. Skinner ◽  
Thomas Nann
Keyword(s):  
Magnetic Properties ◽  
Iron Oxide ◽  
Phase Transfer ◽  
Synthesis Method ◽  
Superparamagnetic Iron Oxide ◽  
Spherical Particles ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Water Dispersible

Cube-shaped magnetic iron oxide nanoparticles were synthesised and studied with the aim to achieve superior magnetic properties. This study describes a straightforward and simple synthesis method for preparing monodisperse 11–14-nm superparamagnetic iron oxide nanocubes via an ‘effective monomer’ growth mechanism. The as-synthesised nanoparticles are insoluble in water. However, substitution of the non-polar ligands of the particles using a new method that involved an ionic compound generated colloidally stable and water dispersible cube-shaped particles with a very small hydrodynamic diameter. The cubes displayed superior magnetic properties over spherical particles.

scholarly journals Cellular Investigations on Mechanistic Biocompatibility of Green Synthesized Calcium Oxide Nanoparticles with Danio rerio

2021 ◽  
Vol 2 (1) ◽  
pp. 51-62
Author(s):  
Rashke Eram ◽  
Puja Kumari ◽  
Pritam Kumar Panda ◽  
Sonal Singh ◽  
Biplab Sarkar ◽  
...  
Keyword(s):  
Green Synthesis ◽  
Calcium Oxide ◽  
Leaf Extract ◽  
Molecular Level ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Adult Zebrafish ◽  
Molecular Investigation ◽  
Physical Fields

The utility of calcium oxide nanoparticles in the biomedical and physical fields has instigated their biocompatible synthesis and production. Moreover, it is important to investigate their biocompatibility at the molecular level for biomedical and ecotoxicological concern. This study explores the green synthesis of calcium oxide nanoparticles (CaONP) using Crescentia cujete leaf extract. The synthesized CaONP were found to have a size of 62 ± 06 nm and a hydrodynamic diameter of 246 ± 12 nm, as determined by FE-SEM and dynamic light scattering (DLS). CaONP was stable in fish medium with a zeta potential of −23 ± 11 mV. The biocompatibility of the CaONP was investigated with adult zebrafish bearing an LC50 of 86.32 µg/mL. Cellular and molecular investigation revealed the mechanism of biocompatibility as a consequence of elicited reactive oxygen species leading to apoptosis, due to accumulation and internalization of CaONP in exposed zebrafish. The study provided detailed information about the mechanistic biocompatibility and a defined horizon of green synthesis of CaONP for biomedical and ecological purposes.

scholarly journals Biocompatible Layers Obtained from Functionalized Iron Oxide Nanoparticles in Suspension

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 773 ◽  
Author(s):  
Daniela Predoi ◽  
Simona Liliana Iconaru ◽  
Mihai Valentin Predoi ◽  
Nicolas Buton ◽  
Christelle Megier ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Characterization Methods ◽  
Sem Image ◽  
Stable Suspension ◽  
Cytotoxicity Studies ◽  
Ultrasound Measurements ◽  
The Stability

Iron oxide nanoparticles have been extensively studied for challenges in applicable areas such as medicine, pharmacy, and the environment. The functionalization of iron oxide nanoparticles with dextran opens new prospects for application. Suspension characterization methods such as dynamic light scattering (DLS) and zeta potential (ZP) have allowed us to obtain information regarding the stability and hydrodynamic diameter of these suspended particles. For rigorous characterization of the suspension of dextran-coated iron oxide nanoparticles (D-MNPs), studies have been performed using ultrasound measurements. The results obtained from DLS and ZP studies were compared with those obtained from ultrasound measurements. The obtained results show a good stability of D-MNPs. A comparison between the D-MNP dimension obtained from transmission electron microscopy (TEM), X-ray diffraction (XRD), and DLS studies was also performed. A scanning electron spectroscopy (SEM) image of a surface D-MNP layer obtained from the stable suspension shows that the particles are spherical in shape. The topographies of the elemental maps of the D-MNP layer showed a uniform distribution of the constituent elements. The homogeneity of the layer was also observed. The morphology of the HeLa cells incubated for 24 and 48 h with the D-MNP suspension and D-MNP layers did not change relative to the morphology presented by the control cells. The cytotoxicity studies conducted at different time intervals have shown that a slight decrease in the HeLa cell viability after 48 h of incubation for both samples was observed.

Investigation of Magnetic Iron Oxide Nanoparticle Properties with Co-Precipitation Methods under Different Reaction Conditions

2020 ◽  
Vol 10 ◽  
Author(s):  
Sema Salgın ◽  
Uğur Salgın ◽  
Nagihan Soyer
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Morphological Structure ◽  
Precipitation Method ◽  
Iron Oxide Nanoparticle ◽  
Hydrodynamic Diameter ◽  
Oxide Nanoparticles ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Magnetic Iron Oxide ◽  
Co Precipitation

: In this study, magnetic iron oxide nanoparticles were synthesized by cost effective and an easy co-precipitation method. The effects of reaction parameters such as the mole ratio of Fe2+/Fe3+ (1/1, 1/2 and 1/3) and the base type (NaOH and NH4OH) on the average hydrodynamic diameter, zeta potential, chemical and morphological structure and saturation magnetization values of magnetic iron oxide nanoparticles were systematically investigated. Magnetic iron oxide nanoparticles synthesized at the mole ratio of 1/2 using NH4OH base gave better results in terms of particle size and particle shape, magnetization value. In order to prevent the formation of different phases in the co-precipitation method, an alternative approach was developed and the reaction was performed using inert supercritical CO2 atmosphere.

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