scholarly journals Anticonvulsant activity of Nigella sativa, Aegle marmelos and Benincasa hispida in Pentylenetetrazole induced seizure in Swiss albino mice

2016 ◽  
Vol 7 (3) ◽  
pp. 97-102
Author(s):  
Rejeesh Edavan Puthallath ◽  
Lovelyn Joseph ◽  
Kumaraswamy Kademane ◽  
Sudarshanram Narayan Rao
Keyword(s):  
Nigella Sativa ◽  
Ethanolic Extract ◽  
Water Bath ◽  
Control Group ◽  
Absence Seizure ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Swiss Albino Mice ◽  
Benincasa Hispida ◽  
Albino Mice

Aims and Objectives: To test anticonvulsant properties of three medicinal plants with pentylenetetrazole induced seizure test in Swiss albino mice.Materials and Methods: Herbs selected for the work (leaves of Aegle marmelos, seed of Nigella sativa, and fruit of Benincasa hispida) were collected locally from Mangalore, India and were authenticated by a Taxonomist. Aqueous extract of Aegle marmelos leaves (AmAE) and  Ethanolic extract of Nigella sativa seeds (NsEE) were prepared by Soxhlet extraction and separated from solvent using a water bath. Benincasa hispidafruit juice (BhE) was filtered and dried on a water bath. Qualitative phytochemical analysis of the test extracts were done with Preliminary chemical reaction tests, and Liquid chromatography–mass spectrometry (LC/MS). Male Swiss albino mice, 3-4 months of age, weighing 25-30g were used in the study. Extracts and vehicle were administered orally 1 hour prior to inducing convulsions. Test employed for screening anticonvulsant properties was Pentylenetetrazole (PTZ) induced seizure test [60mg/kg/i.p]. Animals were treated with extracts at a dose of 900mg/kg orally. The results were compared with control group and test for analysis of variance and significance were done (ANOVA, Dunnett’s test).Results: Aqueousextract of Aeglemarmelos leaves and Ethanolic extract of Nigella sativa seeds possess anticonvulsant property in Pentylenetetrazole (PTZ) induced seizure model, Crude extract of Benincasahispida fruits did not show statistically signifi cant anticonvulsant property.Conclusions: Aeglemarmelos leaf and Nigella sativa seed possess anticonvulsant propertiesin pentylenetetrazole induced seizures, this is in accordance with many other publishedreports on the same herbs with different animal models and indicates effectiveness inhuman absence seizure patients.Asian Journal of Medical Sciences Vol. 7(3) 2016 97-102

scholarly journals Antiplasmodial and haematological effects of Senna occidentalis leaf ethanolic extracts on Swiss albino mice infected with Plasmodium berghei

2021 ◽  
Vol 12 (2) ◽  
pp. 122-128
Author(s):  
S.M. Babangida ◽  
G.J. Sow ◽  
D.M. Shehu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Leaf Extract ◽  
White Blood Cells ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Swiss Albino Mice ◽  
Ethanolic Extracts ◽  
Albino Mice

A research was conducted to investigate the haematological effects of ethanolic leaf extracts of Senna occidentalis on Swiss albino mice infected with 0.2 ml of Plasmodium berghei infected blood. Fifteen (15) mice weighing between 140-260g were assigned into five study groups of three mice each. The first group is treated with 0.2 mL of normal saline (drug free control). Group 2, 3, 4 were treated with 100, 200, and 400mg/kg of theethanolic leaf extract respectively while group 5 received 10mg/kg of chloroquine phosphate. All doses were administered orally. The results obtained were analyzed using Analysis of Variance with Duncan’s Multiple Range Test to separate the means. The result of the preliminary phytochemical analysis revealed the presence of alkaloids, saponins, cardiac glycosides, diterpenoids, flavonoids, steroids, tannins, Triterpenoids, carbohydrates and proteins. The level of parasite suppression ranges from 35% to 75% and the activity increased with increase in concentration of the extracts (dose dependent). The extracts were found to increase the level of some haematological parameters such as Red Blood Cells, White Blood Cells and Haemoglobin. The effect is concentration dependent, increases with increase in concentration. Thus, the anti plasmodial efficacy of the leaf extract of S. occidentalis on P. berghei is confirmed. It is recommended that , 400 mg/kg leaf ethanolic extracts of S. occidentalis couldbe use in the treatment of malarial fever.

scholarly journals EFFECT OF ETHANOLIC EXTRACT OF MIMOSA PUDICA ROOT L. ON ACUTE RESTRAINT STRESS-INDUCED ANXIETY AND DEPRESSION IN SWISS ALBINO MICE

2018 ◽  
Vol 11 (10) ◽  
pp. 182 ◽  
Author(s):  
Shashikumara Shashikumara ◽  
Prathima C ◽  
Amrutheswari B
Keyword(s):  
Restraint Stress ◽  
Forced Swim Test ◽  
Ethanolic Extract ◽  
Antidepressant Activity ◽  
Phytochemical Analysis ◽  
Mimosa Pudica ◽  
Swiss Albino Mice ◽  
Acute Restraint Stress ◽  
Immobility Time ◽  
Albino Mice

Objective: The present study is undertaken to study the ameliorative effects of ethanolic extract of Mimosa pudica root (EMPR) on stress-induced anxiety and depressive-like behavior in Swiss albino mice.Methods: The animals were subjected to acute restraint stress (ARS) for 7 days, and on the 8th day after drugs administration, the antianxiety effect was evaluated using elevated plus maze (EPM) test. Antidepressant activity was evaluated by animal despair models-forced swim test and tail suspension test. Phytochemical analysis of the extract was also conducted.Results: EMPR (1000 and 2000 mg/kg) and the combined test groups have shown significant antianxiety effects similar to the standard diazepam as indicated by an improved open arm exploratory behavior in EPM model. EMPR also significantly reduced the immobility time in the animal despair models tested.Conclusion: Results suggest that EMPR possesses significant anxiolytic and antidepressant activity in male Swiss albino mice.

scholarly journals Drug Interaction analysis-Nigella sativa L.seed (Black Cumin) ethanolicextract on antiseizure activity of Phenobarbitone sodium

2020 ◽  
Vol 11 (2) ◽  
pp. 18-20
Author(s):  
Lovelyn Joseph ◽  
Rejeesh Edavan Puthillath ◽  
Sudarshanram Narayan Rao
Keyword(s):  
Interaction Analysis ◽  
Nigella Sativa ◽  
Ethanolic Extract ◽  
Maximal Electroshock ◽  
Maximal Electroshock Seizure ◽  
Multidisciplinary Therapy ◽  
Swiss Albino Mice ◽  
Isobolographic Analysis ◽  
Albino Mice ◽  
Nigella Sativa Seed

Background: Multidisciplinary therapy is gaining popularity and drug herb interactions is one of the biggest risk factor contributing to therapeutic failures or toxicities. Apart from analysis of one of such Drug-Herb combinations, this work also aims at emphasizing need for ethnopharmacovigilance practices. Aims and Objectives: Herb-Drug Interaction analysis between Nigella sativa seed ethanolic extract and Phenobarbitone sodium in maximal electroshock seizure in Swiss albino mice with a modified isobolographic analysis. Materials and Methods: Experiment model was Maximal electroshock seizure in Swiss albino mice. Dose ratios of the combination tested were NsEE: Phenobarbitone; 3:1(NsEE75%: Pbt25%), 1:1, 1:3. Analysis was done with modified isobolographic analysis. Results: Nigella sativa seed ethanolic extract exhibit synergistic interaction with Phenobarbitone sodium at 1:1 and 1:3 dose ratios and antagonistic interaction at 3:1 combination. The parameter measured was Hind limb tonic extensor phase in maximal electroshock seizure test in Swiss albino mice. Conclusion: The study showed that interaction profile of Nigella sativa extract-phenobarbitone combination is dose dependent and requires well designed posological studies in epileptic patients to formulate dose adjustment guidelines for multidisciplinary therapy with the herb and Phenobarbitone, also to avoid food drug interactions.

scholarly journals Anti-anxiety activity of ethanolic extract of Spermacoce hispida leaves in swiss albino mice

2017 ◽  
Vol 6 (9) ◽  
pp. 2224
Author(s):  
Vinayak Meti ◽  
Kumarswamy Dabburu
Keyword(s):  
Ethanolic Extract ◽  
Experimental Models ◽  
Anxiolytic Activity ◽  
Control Group ◽  
Swiss Albino Mice ◽  
Test Models ◽  
Chronic Study ◽  
Albino Mice ◽  
Post Hoc ◽  
Better Than

Background: The objective of the study was to evaluate the anxiolytic activity of ethanolic extract of Spermacoce hispida (EESH) in swiss albino mice.Methods: Forty eight adult Swiss albino mice weighing 25-30grams were selected. 24 were allocated to Elevated plus maize test and the remaining 24 to Bright and dark box test models. In each model, there were 4 groups. The control group received normal saline (10ml/kg), the standard, Diazepam (1mg/kg) and test groups received ethanolic extract of Spermacoce hispida (100mg/kg, 200mg/kg p.o. respectively) 1h prior to the acute study. In chronic study the drugs were given orally once a day for 10 days and the last dose was given 1h before the experiment. The antianxiety activity of the test drug was evaluated using EPM test and Bright and dark box test experimental models of anxiety. All results were expressed as mean±standard error mean (SEM) and analysed by one-way ANOVA. Post-hoc comparisons were performed by applying Dunnet’s test. P <0.05 was considered statistically significant.Results: Ethanolic extract of Spermacoce hispida significantly increased the duration of time spent and number of entries in open arm in EPM and increased the number of entries into the bright arena, the time spent and the rears in bright arena indicating anxiolytic activity and EESH 200 mg/kg was found to be better than diazepam.Conclusions: The results of the present study suggest that the ethanolic extract of Spermacoce hispida have anxiolytic activity better than Diazepam.

scholarly journals EVALUATION OF ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT OF ALANGIUM SALVIFOLIUM LEAVES IN ANIMAL MODELS

2019 ◽  
pp. 397-400
Author(s):  
PRATHIMA C ◽  
SHASHIKUMARA SHASHIKUMARA ◽  
THIPPESWAMY T
Keyword(s):  
Body Weight ◽  
Anticancer Activity ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Swiss Albino Mice ◽  
Soxhlet Apparatus ◽  
Albino Mice

Objective: The present study aims to evaluate the anticancer potential of ethanolic extract of Alangium salvifolium leaves (EASL) against Ehrlich ascites carcinoma (EAC) in animal model. Methods: The ethanolic extract of A. salvifolium was prepared by continuous method using Soxhlet apparatus. Phytochemical analysis of EASL was conducted. Anticancer activity of the extract was evaluated by EAC model in Swiss albino mice. EASL was administered at the doses of 100 and 200 mg/kg body weight once a day orally for 14 days, after 24 h of tumor inoculation. Results: A. salvifolium leaves extract treatment produced significant decrease in tumor volume and body weight, and increase in total and non-viable cell count, along with elevated life span of EAC-bearing mice. The EASL exhibited potent dose-dependent antitumor activity. Further, the altered hematological parameters such as red blood cells, hemoglobin, and white blood cells levels as well as biochemical parameters such as serum glutamic oxaloacetic transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, albumin, and total protein levels in EAC mice were significantly (p<0.05) regained following treatment with EASL. The anticancer potential of EASL can be attributed to the presence of phenols and flavonoids. Conclusion: Results suggest that EASL possesses significant anticancer activity against EAC tumor-bearing Swiss albino mice.

Effect of Sorafenib on Sex Hormone Levels in Male Swiss Albino Mice

2021 ◽  
pp. 3883-3888
Author(s):  
Surekha D. Shetty ◽  
Laxminarayana Bairy K. ◽  
AM Prasad ◽  
Satheesha Nayak B. ◽  
Ashwini Aithal P.
Keyword(s):  
Body Weight ◽  
Semen Analysis ◽  
Young Patients ◽  
Differentiated Thyroid Carcinoma ◽  
Positive Control ◽  
Vital Role ◽  
Reproductive Age ◽  
Control Group ◽  
Swiss Albino Mice ◽  
Albino Mice

Background: Hormones play a vital role in initiating and maintenance of male reproductive or testicular function which includes the production of androgens and spermatozoa. Testosterone is essential for the initiation and maintenance of spermatogenesis. FSH is responsible for the stimulation of spermatogenesis. Semen analysis and hormone evaluation are essential parameters in the diagnosis of infertility in males. Objective: The aim of the present study is to evaluate the effect of sorafenib on FSH and intratesticular testosterone levels in male Swiss albino mice. Materials and Methods: The animals were segregated into control, positive control, and treatment groups (n=6). Treatment group received 25, 50 and 100 mg/kg body weight of sorafenib orally for seven consecutive days at intervals of 24 hours between two administrations. Positive control group received 100 mg/kg body weight of imatinib. The animals were sacrificed at the end of 1st, 2nd, 4th, 5th, 7th and 10th week after the last exposure to sorafenib. Results: The intratesticular testosterone level was significantly (P<0.05) reduced in treated groups and severe effect was observed on week 4th and 5th weeks. FSH level was increased significantly (P<0.05) in sorafenib treated groups of mice. Conclusion: The administration of sorafenib does affect testosterone and FSH level significantly, but this effect is reversible once the drug is withdrawn. This finding may help the clinicians to plan and address the fertility-related issues in young patients of reproductive age who are being treated with sorafenib for advanced renal cell carcinoma, hepatocellular carcinoma and differentiated thyroid carcinoma.

scholarly journals Effect of aqueous Extract of Terminalia bellirica fruit pulp on Alcohol affected learning in swiss albino mice

2021 ◽  
Vol 6 (2) ◽  
pp. 76-78
Author(s):  
Sowmya ◽  
Manohar VR ◽  
Mohandas Rai ◽  
H N Gopalakrishna ◽  
Chandrashekar R
Keyword(s):  
Aqueous Extract ◽  
Protective Action ◽  
Negative Control ◽  
Control Group ◽  
Acute Administration ◽  
Aqueous Extracts ◽  
Swiss Albino Mice ◽  
Alcohol Administration ◽  
Group I ◽  
Albino Mice

To evaluate the effect of Aqueous extract of Terminalia belliricafruit pulp (AETB) on learning by Hebb William maze model in mice with acute alcohol consumption.Swiss albino mice (n=48) of either sex weighing 20-30g will be divided into eight groups of six mice each. Drugs were given orally after 12 hours of fasting. Group I mice received 10ml/kg of Normal Saline, Group II mice received Piracetam 200mg/kg, Group III received AETB 36mg/kg, Group IV received ethanol 1.5g/kg orally, Group V received ethanol(1.5g/kg )+ piracetam (200mg/kg), Group VI mice received ethanol(1.5g/kg) +AETB(9mg/kg), Group VII mice received ethanol(1.5g/kg) +AETB (18mg/kg), Group VIII mice received ethanol(1.5g/kg) +AETB(36mg/kg). Time taken by the animal to reach the reward chamber from the start chamber (TRC) in Hebb-William maze was used as a parameterto evaluate the learning.Acute alcohol administration showed increase in TRC. Whereas, acute administration of Aqueous extracts of Terminalia belliricafruit pulp showed a decrease in TRC when compared to the control group. The TRC values for the groups that were administered AETB along with acute alcohol administration showed decrease in TRC values compared to the negative control.Current study showed acute alcohol administration caused impairment of thelearning ability in mice. Whereas, acute administration of Aqueous extracts of Terminalia belliricafruit pulp (AETB)caused enhancement of learning. Pre-treatment with AETB before acute alcohol administration indicated protective action of AETB on alcohol affected learning in mice.

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