scholarly journals Photosynthetic, Biochemical and Enzymatic Investigation of Azolla microphylla in Response to an Insecticide-Hexachlorohexahydro-Methano-Benzodioxathiepine-Oxide

Our Nature ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 145-155
Author(s):  
Waseem Raja ◽  
Preeti Rathau ◽  
Suchit A. John ◽  
P.W. Ramteke

A study on the heterocystous, nitrogen fixing Water fern, Azolla microphylla was carried out to investigate the effect of an organochlorine insecticide (hexachloro-hexahydro-methano benzodioxathiepine-oxide, called as endosulfan) at different concentrations of 0, 50, 100, 200, 400 and 600 ppm on fresh weight, dry weight, photosynthetic pigments, stress metabolites such as ascorbic acid, proteins, and nitrogen metabolism activity like nitrate reductase and nitrate uptake. The inhibition was found to be dose dependent. The insecticide endosulfan showed to be deleteriously affecting the activities in the Azolla microphylla. Endosulfan adversely depleted the cellular activities, leading to a marked increase in the Vitamin-C at lower concentration and gradually decreases at higher concentrations. Decrease in protein was clear and activities like nitrate reductase and nitrate uptake also increases up to certain concentration and at higher concentration slightly decreases. Despite of deleterious effects of endosulfan on the Azolla microphylla, a unique regenerating ability in presence of the insecticide was observed by the end of five days in the lower doses of insecticide. Azolla seems to help sustain the soil nitrogen supply by returning nitrogen to quantities roughly equal to those extracted from the soil by the rice plant.DOI: http://dx.doi.org/10.3126/on.v10i1.7775

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1116C-1116
Author(s):  
Rebecca Darnell

Most Vaccinium species, including V. corymbosum, have strict soil requirements for optimal growth, requiring low pH, high iron, and nitrogen, primarily in the ammonium form. V. arboreum is a wild species adapted to high pH, low iron, nitrate-containing soils. This broader soil adaptation in V. arboreum may be related to increased efficiency of iron or nitrate uptake/assimilation compared with cultivated Vaccinium species. To test this, nitrate and iron uptake, and nitrate reductase (NR) and ferric chelate reductase (FCR) activities were compared in two Vaccinium species, V. arboreum and the cultivated V. corymbosum. Plants were grown hydroponically for 15 weeks in either 1.0 or 5.0 mm NO3 with 0.09 mm Fe. Root FCR activity was greater in V. arboreum compared with V. corymbosum, especially at the lower external nitrate concentration. However, this was not reflected in differences in iron uptake. Nitrate uptake and root NR activity were greater in V. arboreum compared with V. corymbosum. The lower nitrate uptake and assimilation in V. corymbosum was reflected in decreased plant dry weight compared with V. arboreum. V. arboreum appears to be more efficient in acquiring nitrate compared with V. corymbosum, possibly due to increased NR activity, and this may partially explain the wider soil adaptation of V. arboreum.


2010 ◽  
Vol 18 (6) ◽  
pp. 1157-1162
Author(s):  
Shu-Jun ZHAO ◽  
Jia-Fu YUAN ◽  
Xin-Ran ZHANG ◽  
Xiang-Yu XU ◽  
You-Sheng XIONG ◽  
...  

2020 ◽  
Vol 117 (3) ◽  
pp. 351-365
Author(s):  
J. Pijlman ◽  
G. Holshof ◽  
W. van den Berg ◽  
G. H. Ros ◽  
J. W. Erisman ◽  
...  

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 182
Author(s):  
Ruchi Bansal ◽  
Swati Priya ◽  
Harsh Kumar Dikshit ◽  
Sherry Rachel Jacob ◽  
Mahesh Rao ◽  
...  

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.


Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.


2012 ◽  
Vol 10 (1) ◽  
pp. 16-22 ◽  
Author(s):  
M. Z. U. Kamal ◽  
M. N. Yousuf

The investigation was carried out to evaluate the effect of different organic manures on turmeric with reference to vegetative growth, biomass production, rhizome yield and its attributes of turmeric (Curcuma longa L.). Turmeric showed better response to the application of organic manures. Plant with neem cake application had the taller plant (79.30 cm), maximum number of tillers per plant (5.40), leaf number (5.40), leaf area (44.09) leaf area index (0.429), fresh weight of halum ( 190.05g), fresh weight of root (49.13 g), fresh weight of rhizome per plant (256.21 g) and dry weight of halum (15.21g), dry weight of root (7.32 g), dry weight of rhizome per plant (40.35 g), total dry matter yield (6.85 t ha-1) than those received other types of manures. Moreover, yield attributes such as number of mother rhizomes per plant-1 (1.75), more number of primary rhizomes per plant-1 (5.19), secondary rhizomes per plant-1 (18.03) and tertiary rhizomes per plant (7.69) were also highly accelerated by neem cake application. Similarly, the same treatment expressed the best in terms of size of mother rhizome (7.69 cm), primary rhizome (21.86 cm) and secondary rhizomes (7.05 cm).All these parameters in cumulative contributed to  produce the highest estimated fresh rhizomes yield & cured rhizomes yield (29.48 t ha-1, 5.59 t ha-1 respectively). The highest curing percentage (20.28) was observed in T3 treatment having mustard cake@ 2.0 t/ha. Thus, organic manure like neem cake was best fitted natural fertilizer for turmeric cultivation.DOI: http://dx.doi.org/10.3329/agric.v10i1.11060The Agriculturists 2012; 10(1): 16-22


2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.


1987 ◽  
Vol 65 (11) ◽  
pp. 2822-2824 ◽  
Author(s):  
W. A. Montevecchi ◽  
J. F. Piatt

We present evidence to indicate that dehydration of prey transported by seabirds from capture sites at sea to chicks at colonies inflates estimates of wet weight energy densities. These findings and a comparison of wet and dry weight energy densities reported in the literature emphasize the importance of (i) accurate measurement of the fresh weight and water content of prey, (ii) use of dry weight energy densities in comparisons among species, seasons, and regions, and (iii) cautious interpretation and extrapolation of existing data sets.


1981 ◽  
Vol 45 (2) ◽  
pp. 283-294 ◽  
Author(s):  
Ann-Sofie Sandberg ◽  
H. Andersson ◽  
B. Hallgren ◽  
Kristina Hasselblad ◽  
B. Isaksson ◽  
...  

1. An experimental model for the determination of dietary fibre according to the definition of Trowell et al. (1976) is described. Food was subjected to in vivo digestion in ileostomy patients, and the ileostomy contents were collected quantitatively, the polysaccharide components of which were analysed by gas–liquid chromatography and the Klason lignin by gravimetric determination. The model was used for the determination of dietary fibre in AACC (American Association of Cereal Chemists), wheat bran and for studies on the extent of hydrolysis of wheat-bran fibre in the stomach and small intestine. The effect of wheat bran on ileostomy losses of nitrogen, starch and electrolytes was also investigated.2. Nine patients with established ileostomies were studied during two periods while on a constant low-fibre diet. In the second period 16 g AACC wheat bran/d was added to the diet. The ileostomy contents and duplicate portions of the diet were subjected to determinations of wet weight, dry weight, water content, fibre components, starch, N, sodium and potassium.3. The wet weight of ileostomy contents increased by 94 g/24 h and dry weight by 10 g/24 h after consumption of bran. The dietary fibre of AACC bran, determined as the increase in polysaccharides and lignin of ileostomy contents after consumption of bran, was 280 g/kg fresh weight (310 g/kg dry matter). Direct analysis of polysaccharides and lignin in bran gave a value of 306 g/kg fresh weight. Of the added bran hemicellulose and cellulose 80–100% and 75–100% respectively were recovered in ileostomy contents. There was no significant difference between the two periods in amount of N, starch and K found in the ileostomy contents. The Na excretion increased during the ‘bran’ period and correlated well with the wet weight of ileostomy contents.4. In conclusion, it seems probable that determination of dietary fibre by in vivo digestion in ileostomy patients comes very close to the theoretical definition of dietary fibre, as the influence of bacteria in the ileum seems small. Bacterial growth should be avoided by using a technique involving the change of ileostomy bags every 2 h and immediate deep-freezing of the ileostomy contents. True dietary fibre can be determined by direct analysis of polysaccharides and lignin in the food, at least in bran. Very little digestion of hemicellulose and cellulose from bran occurs in the stomach and small bowel. The 10–20% loss in some patients may be due to digestion by the gastric juice or to bacterial fermentation in the ileum, or both. The extra amount of faecal N after consumption of bran, reported by others, is probably produced in the large bowel.


2021 ◽  
Vol 27 (1) ◽  
pp. 1-12
Author(s):  
Bilal Ahmad Khan ◽  
Saima Anwar ◽  
Rizwan Maqbool Maqbool ◽  
Muhammad Mohsin Amin ◽  
Muhammad Mansoor Javaid ◽  
...  

ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory


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