scholarly journals The Effect of Jatropha Curcas L Seed Extract in Oxidatve Stress and Central Vein of Liver Tissue

2020 ◽  
Vol 5 (1) ◽  
pp. 41
Author(s):  
Putri Nurbaeti ◽  
Rr Ayu Fitri Hapsari ◽  
Endah Wulandari
Keyword(s):  
Seed Extract ◽  
Central Vein ◽  
Central Metabolism ◽  
Toxic Compounds ◽  
Tropical Plant ◽  
Vein Diameter ◽  
Liver Homogenates ◽  
High Doses ◽  
Jatropha Seed ◽  
Hepatocyte Cells

Introduction:Jatropha curcas L is a tropical plant, besides containing antioxidants it also contains toxic compounds. This plant is believed to be anti-inflammatory, antimicrobial, antibacterial and antifertilization. It is suspected that this plant can damage tissues through oxidative stress. The liver as an organ of central metabolism and detoxification. This plant is not yet known its effect on the liver when consumed, especially on hepatocyte cells and blood flow to the central veins of the liver.Method: Mice were given a dose of jatropha seed extract (0, 5, 25, 50, 250 mg/KgBW) for 28 days. Liver homogenates were measured for MDA levels, GSH activity and their correlation using a spectrophotometry. besides measuring the diameter of the central vein of the liver. Results: MDA levels after being given Jatropha seed extract: control (0); 5; 25; 50; 250 mg/KgBW were 0.818; 1,363; 2,043; 2,720; 2,518 nmol / ml and GSH activity was 0.979; 0675; 0.621; 0.463; 0.544 μg / ml. Vein diameter 169; 140; 24; 36; 30 μm and the smallest at a dose of 25 mg / BW was 23.7 μm (ANOVA, p <0.05). The correlation of MDA and GSH levels was very strong negative (Pearson: -0918; p <0.01). Discussion:The higher dose of jatropha seed extract, significantly increases MDA levels and decreases GSH activity. The correlation between the two is very strong negative negative correlation is very significant. Jatropha seed extract with high doses causes small central vein diameters of the liver.International Journal of Human and Health Sciences Vol. 05 No. 01 January’21 Page: 41-46

Anti-herpes simplex virus effect of a seed extract from the tropical plant Licania tomentosa (Benth.) Fritsch (Chrysobalanaceae)

Phytomedicine ◽  
2002 ◽  
Vol 9 (7) ◽  
pp. 641-645 ◽  
Author(s):  
M.M.F.S. Miranda ◽  
J.L.S. Gonçalves ◽  
M.T.V. Romanos ◽  
F.P. Silva ◽  
L. Pinto ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Seed Extract ◽  
Tropical Plant ◽  
Simplex Virus

scholarly journals The Effect of Jatropha curcas L Seed Extract on AST/ALT Activity and The Central Vein Thickness in Liver

2021 ◽  
Vol 13 (1) ◽  
pp. 66-72
Author(s):  
Faradila Amirabagya ◽  
Rr Ayu Fitri Hapsari ◽  
Endah Wulandari
Keyword(s):  
Jatropha Curcas ◽  
Seed Extract ◽  
Central Vein ◽  
Jatropha Curcas L ◽  
Alt Activity

scholarly journals Pengaruh Perbedaan Dosis Ekstrak Biji Jarak Pagar (Jatropha curcas) terhadap Jumlah Spermatozoa, Spermatozoa Motil, Berat Testis, dan Diameter Testis pada Mencit Jantan (Mus Musculus)

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Haifa Wahyu ◽  
Rahmatina B. Herman ◽  
Arni Amir
Keyword(s):  
Mus Musculus ◽  
Jatropha Curcas ◽  
Sperm Count ◽  
Seed Extract ◽  
Control Group ◽  
Testis Weight ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Jatropha Seed

AbstrakIndonesia menghadapi persoalan kependudukan dan keluarga berencana yang cukup berat, salah satu penyebabnya dikarenakan rendahnya partisipasi pria dalam penggunaan kontrasepsi. Salah satu tanaman yang bersifat antifertilitas adalah jarak pagar (Jatropha curcas). Penelitian ini bertujuan mengetahui pengaruh perbedaan dosis ekstrak biji jarak pagar terhadap jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis pada mencit jantan (Mus musculus). Penelitian ini menggunakan metode post test only control group design terhadap mencit jantan dengan berat 20–30 gram. Sampel terdiri dari 24 ekor mencit dibagi dalam 3 kelompok P1, P2, dan P3 dengan memberikan ekstrak biji jarak pagar dengan 3 tingkatan dosis selama 36 hari. Hasil penelitian semakin tinggi dosis yang diberikan semakin rendah jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis namun secara statistik tidak terdapat pengaruh yang bermakna pada jumlah spermatozoa p=0,06 dan spermatozoa motil p=0,15 (p > 0,05) dan terdapat pengaruh bermakna pada berat testis p=0,00 dan diameter testis p= 0,00 (p< 0,05).Kesimpulannya pemberian ekstrak biji jarak pagar menurunkan jumlah spermatozoa, spermatozoa motil, berat testis dan diameter testis namun secara statistik ekstrak biji jarak pagar tidak terdapat pengaruh yang bermakna terhadap jumlah spermatozoa dan spermatozoa motil dan terdapat pengaruh bermakna terhadap berat testis dan diameter testis.Kata kunci: ekstrak biji jarak pagar, jumlah spermatozoa, spermatozoa motil, berat testis, diameter testis.AbstractIndonesia faces the quite severe problem of population and family planning, one reason is because the low participation of men in the use of contraception. One antifertility plants and are found in Indonesia is jatropha (Jatropha curcas). This study aims to determine the effect of different doses of extract of Jatropha seeds on sperm count, motile of sperm, testis weight and diameter of the testis in male mice (Mus musculus). This study uses the approach post test only control group design to male mice weighing 20-30 grams. The sample consisted of 24 mice were divided into 3 groups of P1, P2, and P3 to provide jatropha seed extract with 3 levels of doses for 36 days. The results showed the effect of Jatropha curcas seed extracts against sperm count, motile of sperm, testicular weight and testicular diameter between groups P1, P2 and P3. The higher the dose given, the lower the sperm count, motile of sperm, testis weight and diameter of the testis, but no statistically significant effect on the number of spermatozoa p = 0.06 and p = 0.15 motile of sperm (p> 0.05 ), and there is a significant effect on testis weight p = 0.00 and p = 0.00 testis diameter (p <0.05). From the results of this study concluded that administration of Jatropha seed extract can reduce sperm count, motile of sperm, testis weight and diameter of the testis but statistically jatropha seed extract contained no significant effect on sperm count and motile of sperm and significant effect on testis weight and the diameter of the testes.Keywords: Jatropha seed extract, sperm numbers, motile of sperm, testis weight, testis diameter.

scholarly journals Cadmium induced hepatic intoxication and amelioration by grape seed extract

2017 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Adel Adel Alkhedaide ◽  
Gehan Youssef ◽  
Rabab El-Zoghby ◽  
Mohamed Mahmoud ◽  
Shimaa Atwa
Keyword(s):  
Wistar Rats ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Degenerative Changes ◽  
Control Group ◽  
Central Vein ◽  
Stomach Tube ◽  
Male Adult ◽  
Hydropic Degeneration

Cadmium (Cd) is wide-spread toxic metal that pollutes most of the vegetables, which eaten by numerous populations all over the world. The aim of the current work is to evaluate the protective and prophylactic effects of the antioxidant materials in the grape seed extract (GSE) on the hepatic intoxication induced by cadmium chloride toxic material in male Wistar rats. Male adult Wistar rats were divided into four groups. Control group fed on balanced diet and given drinking water. Group two (control positive) given CdCl2 in the dose of 0.44 mg/kg body weight (BW) by stomach tube daily. Group three given grape seed extract GSE in the dose of (100 mg/kg BW) daily by stomach tube. Finally, the fourth group gave mixture of (GSE and CdCl2) by stomach tube in the recommended dose. Blood and liver tissues were collected for further biochemical and histopathological studies. CdCl2 significantly increased the serum levels of malondialdehyde, ALT and AST. Cadmium administration decreased levels of antioxidants (Catalase, GSH-R and GSH-Px). The liver of the control positive groups which given CdCl2 showed degenerative changes in the form of vacuolar and hydropic degeneration. Congestion was evident in the central vein and proliferation of the kupffer cells. These hepatic biochemical and degenerative changes were ameliorated by the co addition of GSE.

scholarly journals Reproducibility of point-of-care ultrasonography for central vein diameter measurement: Separating image acquisition from interpretation

2017 ◽  
Vol 45 (8) ◽  
pp. 488-496 ◽  
Author(s):  
Brian P. Lucas ◽  
Antonietta D'Addio ◽  
Jennifer Clark ◽  
Clay Block ◽  
Harold Manning ◽  
...  
Keyword(s):  
Point Of Care ◽  
Image Acquisition ◽  
Central Vein ◽  
Diameter Measurement ◽  
Vein Diameter ◽  
Point Of Care Ultrasonography

scholarly journals Continuous Intake of High Doses of Piper guineense (Ashanti pepper) Aqueous Seed Extract Impairs Renal Function in Wistar Rats

2021 ◽  
Vol 18 (3) ◽  
pp. 585-590
Author(s):  
Somke Pamela Madueke ◽  
Princewill Ikechukwu Ugwu ◽  
Chinaza Amarachgui Uru ◽  
Adaobi Pearl Okeke ◽  
Okechukwu Matthew Omire-Oluedo ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dose Group ◽  
Histological Study ◽  
Seed Extract ◽  
High Dose Group ◽  
High Dose ◽  
Piper Guineense ◽  
Group 2 ◽  
High Doses ◽  
Medium Dose

BACKGROUND: Piper guineense seed is a well known spice consumed in many parts of West Africa as a result of its nutritional and medicinal properties. METHODS: Twenty Wistar rats were divided into four groups of five per group. The phytochemical analyses was done; different concentration of aqueous seed extract of Piper guineense was administered for 21 days to three experimental groups, Group 2 (25 mg/kg), Group 3 (50 mg/kg), Group 4 (100 mg/kg), while group 1 were given only rat feed and water. RESULTS: The investigation revealed that in low and medium dose groups, creatinine (62.3±7.3 to 51.1±4.5 and 51.1±8.1 respectively) and urea (6.6±1.3 to 5.2±0.8 and 4.8±1.0) levels decreased significantly while in high dose group, creatinine (62.3±7.3 to 66.9±11.0) and urea (6.6±1.3 to 7.0±0.8) increased significantly. There was a dose dependent increase in the serum electrolyte (sodium, potassium, chlorine and bicarbonate). Only bicarbonate (19.1±0.8 to 24.3±1.3) and chloride (102.4±3.8 to 107.0±1.6) had significant increase in their values. The histological study showed that at a low (25mg/kg) and medium dose (50mg/kg) of aqueous seed extract of Piper guineense the normal cyto-architecture of the kidney was maintained while in high dose group (100mg/kg) normal cyto-architecture of the kidney was distorted. CONCLUSION: The result obtained suggests that Piper guineense seed may not be harmful at a moderate dose; but high doses could be toxic. Caution should be taken on the quantity of Piper guineense seed consumed.

scholarly journals Histologi, Histomorfometri, dan Histokimia Hati Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas

2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Siswandy Siswandy ◽  
Erdiansyah Rahmi ◽  
Dian Masyitha ◽  
Fitriani Fitriani ◽  
Fadli A. Gani ◽  
...  
Keyword(s):  
Gallus Gallus ◽  
Gallus Domesticus ◽  
Chicken Liver ◽  
Central Vein ◽  
Gallus Gallus Domesticus ◽  
Liver Growth ◽  
Native Chicken ◽  
Significant Difference ◽  
Vein Diameter ◽  
Native Chickens

ABSTRAK. Hati adalah kelenjar terbesar yang terdapat di dalam tubuh dan dapat dianggap sebagai organ pusat dalam pemeliharaan suplai energi. Penelitian ini bertujuan untuk mengetahui histologi, histomorfometri, dan histokimia sebaran glikogen pada hati ayam buras sebelum dan sesudah menetas. Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam hati ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) pertumbuhan hati ayam buras secara makroskopis. Pada setiap perkembangan sudah terdapat hepatosit berbentuk poligon yang memiliki inti sel bulat di tengah hepatosit, terdapat juga vena central yang dilapisi sel endotel pipih selapis serta didapati sinusoid yang berada diantara hepatosit, dan juga ditemukan Kupffer’s cell hanya pada hati ayam setelah menetas hari ke-7. Hasil pengukuran diameter vena central hati ayam buras hari ke-7, hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 17,70 ± 1,16 µm; 60,32 ± 2,22 µm; 49,316 ± 1,93 µm; dan 129,11 ± 7,43 µm. Hasil histokimia sebaran glikogen pada hati ayam buras hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 1,44 ± 0,93; 2,27 ± 0,74; dan 2,77 ± 0,15. Dapat disimpulkan, bahwa diameter vena central dan sebaran glikogen meningkat seiring pertumbuhan ayam buras. (Histological, histomorphometrical, and histochemical of liver on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRACT. The liver is the largest gland in the body and considered as a central organ in maintaining energy supplies. The study aimed to determine the histologic, histomorphometric and histochemical of glycogen distribution in the liver of native chicken during pre and posthatching period.. The study sample were divided into four different age groups and each group consisted of six chicken livers. The results showed a significant difference (P0,05) in native chicken liver growth macroscopically. In every development there are already polygonal hepatocytes that have a round cell nucleus in the middle of the hepatocytes, there is also a central vein that is coated with a layer of flat endothelial cells and also sinusoids that are between the hepatocytes, and kupffer's cells also found in chicken liver 7th day posthatching. The results measurements of the central vein diameter in liver native chickens on the 7th, 14th, 20th pre hatching period and 7th post hatching were 17.70 ± 1.16 µm; 60.32 ± 2.22 µm; 49.32 ± 1.93 µm; and 129.11 ± 7,43 µm; The measure results of the glycogen distribution in liver native chickens on the 14th, 20th pre hatching period and 7th post hatching were 1,44 ± 0,93; 2,27 ± 0,74; and 2,77 ± 0,15. It can be concluded, that central vein diameter and glycogen distribution were increased by growth of native chickens.

scholarly journals Aktivitas Hepatoprotektif Ekstrak Metanol Buah Lakum (Cayratia trifolia (L.) Domin) Terhadap Diameter Vena Sentralis, Lebar Sinusoid dan Berat Hepar Tikus Putih (Rattus novergicus L.) yang Diinduksi Parasetamol

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Rizki Perdana Putri Diah Wulandari Rousdy, Ari Hepi Yanti
Keyword(s):  
Wistar Rats ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Liver Weight ◽  
Hepatoprotective Activity ◽  
Central Vein ◽  
Standard Drug ◽  
Cayratia Trifolia ◽  
Male Wistar Rats ◽  
Vein Diameter

Secondary metabolites of lakum (Cayratia trifolia (L.) Domin) fruit have been evaluated in previous researches. The fruit contains flavonoids, terpenoids and phenols which have antioxidant properties. The aim of this research was to determine the hepatoprotective activity of methanolic extract of lakum fruit in Wistar rats’ liver induced by high doses of paracetamol. Ripe lakum fruits were macerated with methanol as the solvent. Thirty-five male Wistar rats (Rattus novergicus L.) were used in this research and devided into 7 groups (normal, negative, positive, solvent, 115 mg kg-1, 230 mg kg-1, and 345 mg kg-1 of lakum fruit methanolic extract). Results showed that the effect of 230 mg kg-1 of lakum fruit methanolic extract (central vein diameter: 40,20 ± 6,97 µm; sinusoid wide: 5,60 ± 0,94 µm; and liver weight: 6,33 ± 0,98 g) were similar to the effect of silymarin (central vein diameter: 42,99 ± 6,60 µm; sinusoid wide: 4,49 ± 0,44 µm; and liver weight: 6,58 ± 0,88 g) as the standard drug.

Safety Evaluation of Picralima nitida (Akuamma) Seed Extracton Hepatorenal and Haematological Systems of Rats

2020 ◽  
pp. 1-6
Author(s):  
Oyedotun M Oyeleke ◽  
◽  
Johnson O Oladele ◽  
Boyede D Olwookere ◽  
◽  
...  
Keyword(s):  
Protein Profile ◽  
Safety Evaluation ◽  
Serum Levels ◽  
Seed Extract ◽  
The Body ◽  
Albino Rats ◽  
Marked Elevation ◽  
Low Concentrations ◽  
Group A ◽  
High Doses

Background: Plants like herbs has been extensively used for different kinds of nutritional and medicinal purposes, however, scientific studies has shown that these medicinal plants may have deleterious effects on some vital organs of the body if not taken at the recommended doses. Thus, there is a need for safety evaluation of these plants to ascertain their actions on organ’s physiological functions. This present study evaluates the effects of ethanol seed extract of Picralima nitida on the haematological examines and some enzymes activities in the serum of albino rats. Twenty-five rats were equally randomized into five groups. Group A (control) received distilled water while graded doses (50, 100, 150 and 200 mg/kg body weight) of ethanol seed extract of Picralima nitida were administered to rats in groups B, C, D and E respectively for 14 days. Haematological studies were carried out on the rats’ blood samples while concentrations of total protein, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, and creatinine were measured in the serum. Results: Result obtained revealed that treatment of rats with various graded doses of ethanol seed extract of Picralima nitida caused marked increase in urea and creatine compared to the control. Rats treated with 150 and 200mg/kg bw of the extract showed marked elevation in serum levels of the enzymes suggesting that the extract may have adverse effects at this dose. There was also a marked elevation in serum electrolytes of rats treated with 150 and 200mg/kg bw of the extract. Rats treated with low concentrations (50 and 100 mg/kg bw) of the extract demonstrated no marked differences or alteration in haematological parameters, protein profile and enzyme activities in the serum. Conclusion: The results of this study indicated that consumption of ethanol seed extract of Picralima nitida as a therapeutic treatment is nontoxic at lower dose. However, if taken at high doses, the extract might be detrimental to some vital organs and systems.

scholarly journals Vitis vinifera(Muscat Variety) Seed Ethanolic Extract Preserves Activity Levels of Enzymes and Histology of the Liver in Adult Male Rats with Diabetes

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Nelli Giribabu ◽  
Kilari Eswar Kumar ◽  
Somesula Swapna Rekha ◽  
Sekaran Muniandy ◽  
Naguib Salleh
Keyword(s):  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Normal Activity ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Male Rats ◽  
Activity Levels ◽  
Histopathological Changes ◽  
Liver Homogenates

The effect ofV. viniferaseeds on carbohydrate metabolizing enzymes and other enzymes of the liver in diabetes is currently unknown. We therefore investigated changes in the activity levels of these enzymes followingV. viniferaseed extract administration to diabetic rats.Methods.V. viniferaseed ethanolic extract (250 and 500 mg/kg/day) or glibenclamide (600 μg/kg/day) was administered to streptozotocin-induced male diabetic rats for 28 consecutive days. At the end of treatment, liver was harvested and activity levels of various liver enzymes were determined. Levels of thiobarbituric acid reactive substances (TBARS) were measured in liver homogenates and liver histopathological changes were observed.Results.V. viniferaseed ethanolic extract was able to prevent the decrease in ICDH, SDH, MDH, and G-6-PDH and the increase in LDH activity levels in liver homogenates. The seed extract also caused serum levels of ALT, AST, ALP, ACP, GGT, and total bilirubin to decrease while causing total proteins to increase. Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased. Histopathological changes in the liver were reduced.Conclusion. Near normal activity levels of various enzymes and histology of the liver followingV. viniferaseed ethanolic extract administration may be due to decrease in liver oxidative stress in diabetes.

Sign in / Sign up

Export Citation Format

Share Document