scholarly journals Изучение коллекционного материала озимой твердой пшеницы на наличие гена устойчивости к септориозу Stb2

2020 ◽  
pp. 51-55
Author(s):  
O. S. Zhogaleva ◽  
A. Yu. Myshastaya ◽  
O. S. Pavlenko ◽  
N. N. Vozhzhova ◽  
E. V. Ionova
Keyword(s):  
Durum Wheat ◽  
Resistance Gene ◽  
Pcr Analysis ◽  
North Caucasus ◽  
Septoria Leaf Blotch ◽  
Leaf Blotch ◽  
Caucasus Region ◽  
The North ◽  
Study Results ◽  
High Yields

In order to obtain high yields of winter durum wheat, it is necessary not only to use advanced agricultural technologies, but also to sow varieties resistant to major diseases. One of these diseases that cause great harm to grain crops is Septoria leaf blotch. The current paper has presented the study results of collection material of Russian and foreign winter durum wheat according to the efficiency of the Septoria leaf blotch resistance gene Stb2 in the North Caucasus region. The purpose of the current study was to identify the Septoria leaf blotch resistance gene Stb2 in the collection material of winter durum wheat. The study was carried out in 2017–2018 in the laboratory of marker breeding of the FSBSI “ARC “Donskoy” in Zernograd. In the course of the study there have been applied such methods as the isolation of genomic DNA by the CTAB-method, PCR-analysis with the molecular SSR marker WMS533, electrophoresis on 2% agarose gel, analysis of electrophoresis gels in the Bio-Rad Image Lab 6.0 software, general data analysis by Microsoft Excel. The study has shown that 29 samples out of 185 analyzed ones had the Septoria leaf blotch resistance gene Stb2, they are the lines L3557 h42, L3182 h7, L3335 h29, L3336 h43, L3408 h218 (Russia, FSBSI “Research Center of Plants named after P. P. Lukyanenko”), K-61620 (Russia), K-54455, DF 917 (Romania), K-61924, K-59179, K-51863, K-61869 (Moldova), K-63871 (Turkey), Kharkovskaya 909 (Ukraine), MVTD-15-99 (Hungary), 2196-1/04, 922/93, 589/13, 655/13, 683/13, 145/14, 322/14, 484/14, Gordeiform 1424/82 (Russia, FSBSI “ARC “Donskoy”), Beliy Parus (Ukraine), Belgorodskaya Yantarnaya (LLC “Sativa” in Belgorod, Russia), IR named after V. Ya. Yuriev (Kharkov, Ukraine)), Zhivitsa (Russia, Prikumskaya OSS), Pandur (Romania) and Elidur (Romania). There have been recommended using the identified samples of winter durum wheat with the Septoria leaf blotch resistance gene Stb2 in breeding programs.

SCREENING OF WHEAT LR-GENES FOR RESISTANCE TO PUCCINIA TRITICINA IN THE NORTH CAUCASUS REGION CONDITIONS

1970 ◽  
pp. 54-56
Author(s):  
G. V. Volkova ◽  
O. A. Kudinova ◽  
O. F. Vaganova
Keyword(s):  
Winter Wheat ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
North Caucasus ◽  
Breeding Programs ◽  
Lr Genes ◽  
Caucasus Region ◽  
Highly Efficient ◽  
The North ◽  
Wheat Rust

Currently, more than 70 wheat rust resistance genes are known, but few of them are effective. The purpose of this work is to screen lines of Lr gene carriers for resistance to leaf rust under conditions of the North Caucasus region. Investigations were carried out in 2016-2018 at the infectious site of VNIIBZR. Research material was 49 near isogenic lines of winter wheat cultivar Thatcher. Infectious material was the combined populations of P. triticina, obtained as a result of route surveys of industrial and breeding crops of winter wheat in the areas of the Krasnodar, Stavropol Territories and the Rostov Region, conducted in 2016-2018. According to the assessment, the genes are ranked as follows: - highly efficient genes (plants with no signs of damage): Lr9, Lr42, Lr43 + 24 and Lr50; effective (1R-5R) Lr genes: 19, 24, 29, 36, 37, 38, 45, 47; moderately effective (10MR-20MR) Lr genes: 17, 18, 21, 22a, 28, 32, 41, 52. The remaining Lr-lines were susceptible to P. triticina (25 MR - 90S) to varying degrees. Highly efficient and effective genes Lr9, Lr19, Lr24, Lr29, Lr38, Lr42, Lr43 + 24, Lr47 and Lr50 showed resistance in the seedling phase and can be recommended for inclusion in breeding programs to protect wheat from leaf rust in different phases of plant ontogenesis in the North Caucasus region.

DISTRIBUTION, DIAGNOSIS AND PATHOGENESIS OF RETROVIRAL INFECTIONS IN THE NORTH CAUCASUS REGION

2019 ◽  
Vol 14 (6) ◽  
pp. 946-954
Author(s):  
Sergey Abakin ◽  
◽  
Vladimir Orobets ◽  
Viktor Zaerko ◽  
Inna Klimanovich ◽  
...  
Keyword(s):  
North Caucasus ◽  
Caucasus Region ◽  
The North ◽  
Retroviral Infections

Epizootic situation on invasive diseases in the north Caucasus region

2020 ◽  
Vol 1 (84) ◽  
pp. 261-265
Author(s):  
Oleg Chernykh ◽  
◽  
Alexander Shevchenko ◽  
Lyudmila Shevchenko ◽  
Yuri Drobin ◽  
...  
Keyword(s):  
North Caucasus ◽  
Caucasus Region ◽  
The North ◽  
Invasive Diseases

scholarly journals Transformation and functional verification of Cry5Aa in cotton

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shihao Zhao ◽  
Feng Wang ◽  
Qiuping Zhang ◽  
Jiayi Zou ◽  
Zhangshu Xie ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Insect Resistance ◽  
Expression Patterns ◽  
Instar Larva ◽  
Cotton Bollworm ◽  
Pcr Analysis ◽  
Functional Verification ◽  
Study Results ◽  
Significant Difference ◽  
Transgenic Strains

AbstractMost of the cotton bollworm-resistant genes applied in cotton are more than 20 years and they all belong to Cry1Ab/c family, but the insect-resistant effects of Cry5Aa on cotton were rarely reported. The possible risk of resistance is increasing. The study synthesized a novel bollworm-resistant gene Cry5Aa artificially based on preferences of cotton codon. The new gene was transferred to cotton through the method of pollen tube pathway. The transgenic strains were identified by kanamycin test in field and laboratory PCR analysis. Meanwhile, an insect resistance test was conducted by artificial bollworm feeding with transgenic leaves and GK19 was used as a control in this study. Results showed that rate of positive transgenic strains with kanamycin resistance in the first generation (T1), the second generation (T2) and the third generation (T3) respectively were 7.76%, 73.1% and 95.5%. However, PCR analysis showed that the positive strain rate in T1, T2 and T3 were 2.35%, 55.8% and 94.5%, respectively. The resistant assay of cotton bollworm showed that the mortality rate of the second, third and fourth instar larva feed by the transgenic cotton leaves, were 85.42%, 73.35% and 62.79%, respectively. There was a significant difference between transgenic plant of Cry5Aa and GK19 in insect resistance. Finally, we also conducted the further analysis of gene expression patterns, gene flow and the effect on non-target pest in the study. The results showed that Cry5Aa gene had less environmental impact, and Cry5Aa has been transferred successfully and expressed stably in cotton. Therefore, the novel bollworm resistance gene can partially replace the current insect-resistance gene of Lepidoptera insects.

scholarly journals Resistance of winter wheat varieties to tan spot in the North Caucasus region of Russia

2020 ◽  
Author(s):  
Oksana Yu. Kremneva ◽  
Nina V. Mironenko ◽  
Galina V. Volkova ◽  
Olga A. Baranova ◽  
Yuri S. Kim ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Tan Spot ◽  
North Caucasus ◽  
Wheat Varieties ◽  
Caucasus Region ◽  
The North ◽  
Winter Wheat Varieties

Famous teachers and educators of the first half of the XX century about the problems of the North Caucasian muslim education.

2021 ◽  
Vol 13 (2) ◽  
pp. 208-222
Author(s):  
Nadezhda O. Bleich ◽  
Keyword(s):  
Educational Process ◽  
Point Of View ◽  
Practical Significance ◽  
Past Century ◽  
North Caucasus ◽  
Caucasus Region ◽  
The Past ◽  
The North ◽  
New Type ◽  
Historical Heritage

The article is devoted to the consideration of the worldview positions of famous educators of the past century regarding the state of school education among Muslims of the North Caucasus region. It is proved that the enlighteners advocated the creation of a new type of national non-class school and the construction of the didactic foundations of the educational process in it. The novelty of the work is that, based on the analysis of the views of the advanced intelligentsia of the region, aimed at understanding the current socio-cultural situation, an attempt was made to scientifically understand the problems and prospects for the development of the Muslim educational system of the past from the point of view of the modern scientific paradigm. The practical significance of the publication lies in expanding the understanding of the system of Mohammedan education in the context of its historical heritage, which will help to comprehend modern problems associated with the reform of general and vocational education in the national Muslim republics.

VIRULENCE OF THE NORTH CAUCASIAN POPULATION OF PUCCINIA GRAMINIS PERS. F. SP. TRITICI

1970 ◽  
pp. 40-45
Author(s):  
G. V. Volkova ◽  
Е. V. Gladkova ◽  
O. O. Miroshnichenko
Keyword(s):  
High Efficiency ◽  
Caucasian Population ◽  
Puccinia Graminis ◽  
North Caucasus ◽  
Wheat Varieties ◽  
Caucasus Region ◽  
Pathogen Virulence ◽  
The North ◽  
New Varieties ◽  
Wheat Lines

The aim of the study was to monitor the virulence of Puccinia graminis Pers. f. sp. tritici in the North Caucasus.  The objectives of the study were to collect P. graminis infectious material from sown winter wheat varieties and evaluate the long-term dynamics of the pathogen virulence in the North Caucasus region in 2014-2019. As a result, an analysis of the virulence of the stem rust pathogen population of wheat collected in Krasnodar, Stavropol Territories, and Rostov Region was carried out. 81 mono-empty mushroom isolates were isolated and differentiated.  The genes Sr5, Sr31, Sr38 were characterized by high efficiency.  On the lines with the genes Sr7b, Sr8b, Sr9f, Sr9g, Sr10, Sr11, Sr12, Sr13, Sr14, Sr21, Sr22, Sr23, Sr26, Sr29, Sr32, Sr33, S35, Sr37, SrDp2, SrWLD, a variation in the virulence frequencies of P. graminis was observed.  Significant changes (in the direction of increasing occurrence) in the North Caucasian population 2014-2019  the pathogen was noted in the frequency of clones virulent to wheat lines with resistance genes Sr11, Sr21, Sr22, Sr26, Sr32, Sr33. A decrease in the frequency of clones virulent to Sr8b, Sr9g, Sr10, Sr12, Sr14, Sr35. At approximately the same level, the occurrence of clones virulent to the genes Sr6, Sr7a, Sr8а, Sr9a, Sr9b, Sr9d, Sr9e, Sr13, Sr15, Sr16, Sr17, Sr19, Sr20, Sr24, Sr25, Sr27, Sr30, Sr36, Sr39, Sr40, Sr44, SrGt, SrTmp. Effective genes that have shown their resistance to P. graminis in the seedling phase are proposed for use in breeding in southern Russia to create new varieties of wheat.

CHANGES IN TEMPERATURE REGIME AND PRECIPITATION REGIME OF WARM AND COLD PERIODS IN VARIOUS CLIMATIC ZONES OF THE NORTH CAUCASUS REGION

2021 ◽  
pp. 55-72
Author(s):  
Boris Azretaliyevich Ashabokov ◽  
Lyudmila Mikhailovna Fedchenko ◽  
Lara Asirovna Kesheva ◽  
Nanaliya Vyacheslavovna Teunova
Keyword(s):  
Temperature Regime ◽  
North Caucasus ◽  
Precipitation Regime ◽  
Climatic Zones ◽  
Caucasus Region ◽  
The North

scholarly journals FORECASTING OF DAMAGES FROM NATURAL HAZARDS FOR THE “NORTHERN CAUCASUS RESORTS” TOURIST CLUSTER

2020 ◽  
Vol 12 (3) ◽  
pp. 349-356
Author(s):  
Vyacheslav BABURIN ◽  
◽  
Svetlana BADINA ◽  
Keyword(s):  
Natural Hazards ◽  
Large Scale ◽  
Northern Caucasus ◽  
Individual Risk ◽  
Economic Damage ◽  
North Caucasus ◽  
Limit Values ◽  
Risk Of Death ◽  
The North ◽  
Study Results

The article proposes a methodological approach to potential damage from natural hazards forecasting in case of large-scale investment projects realization in ski tourism planning, as well as to assessing changes in the vulnerability of the territory in which these projects will be implemented. The method was verified on the data of the “Northern Caucasus Resorts” tourist cluster. The study purpose is the creation and verification of a methodology for socio-economic damage predicting in limit values and vulnerability changing in the regions of the “North Caucasus Resorts” tourist cluster objects localization for the long term. Research methods – statistical (a structural approach based on the identification of common structural patterns of several sets). The lack of statistical information on significant parameters for forecasting determine necessitates of using the various logically non-contradictory revaluations based on the identified structural similarities for the calculation of their values within the planning horizon. The study results and main conclusions – in case of the “North Caucasus Resorts” tourist cluster creation the number of people potentially located in avalanche and mudflow danger areas will significantly increase in all of its facilities localization municipalities, which indicate an increase in the individual risk of death level for this territory. The present population in the ski season in some of the most remote and underdeveloped areas can increase up to 30 times. The increment in the value of the fixed assets for the municipalities under consideration will be from two to 90 times, potential damages in limit values will reach tens of billions rubles.

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