scholarly journals A Noninvasive Method for Assessing Oocyte Competency

2020 ◽  
pp. 1-5
Author(s):  
Cara Wessels Wells ◽  
Cara Wessels Wells ◽  
L. Penrose ◽  
M. Orth ◽  
S. Prien
Keyword(s):  
Ethylene Glycol ◽  
Membrane Permeability ◽  
Repeated Measures ◽  
Block Design ◽  
Membrane Integrity ◽  
Oocyte Cryopreservation ◽  
Vitro Fertilization ◽  
Randomized Block Design ◽  
Pre Treatment

There is an increasing demand to evaluate oocyte competency and viability for oocyte cryopreservation and use for in vitro fertilization. Oocytes suffer decreased survival of cryopreservation as compared to embryos, due to physical characteristics of the oocyte. The objective of this study is to determine if a specific gravity device (SGD) can estimate oocyte viability based on oocyte descent through the buoyancy system. All experiments were performed in a research laboratory with a randomized block design with repeated measures. Three hundred-seventy-six oocytes were collected from seven mice and randomly assigned to one of four treatments: exposure to 60C for 30 min, acidified media for 1h, ethylene glycol-glycerol cryoprotectant for 1h, or standard culture to serve as control. To further analyze the relationship between oocyte descent time and viability, 98 additional oocytes were passed through SGD before and after treatment. Oocytes were stained with Coomassie Blue to determine membrane permeability and estimate viability based on treatment. Oocytes treated with 60C heat, acidified media and ethylene glycol/ glycerol cryoprotectant solution demonstrated altered descent times from control and pre-treatment oocytes (P<0.05). Oocytes exposed to heat and cryoprotectants descended more rapidly through SGD than control and pre-treatment oocytes (P<0.05). Oocytes treated with acidified media descended more slowly through SGD (P<0.05). Permeation of stain into oocytes exposed to lethal treatments confirmed changes in membrane integrity post-treatment and further indicates SGD can detect such shifts. This suggests SGD can predict competency between live and dead oocytes. In conclusion, SGD can detect shifts in oocyte density due to altered membrane permeability, which can suggest information about oocyte competency. This information can help differentiate between high- and low-quality fresh oocytes to help select which oocytes to freeze and result in improved oocyte cryopreservation and fertilization.

Lambs produced from cryopreserved sheep embryos derived by in vitro fertilization of aspirated oocytes

1996 ◽  
Vol 76 (3) ◽  
pp. 465-467 ◽  
Author(s):  
N. Songsasen ◽  
A. Martino ◽  
S. P. Leibo ◽  
S. Walmsley ◽  
J. W. Pollard ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ethylene Glycol ◽  
Key Words ◽  
In Vitro Maturation ◽  
Oocyte Cryopreservation ◽  
Vitro Fertilization

A total of 104 oocytes were aspirated from 11 mature ewes and 4 prepubertal lambs. After in vitro maturation, fertilization and culture, 11 of the 104 oocytes developed into blastocysts. These were cryopreserved in 1.5 M ethylene glycol. After being thawed, all were transferred into four recipients, two of which became pregnant and delivered a normal lamb. Key words: Oocyte, cryopreservation, in vitro maturation and fertilization, sheep, embryo

Management of Aries and Aruana Guinea grass cultivars under rotational stocking based on canopy light interception

2021 ◽  
pp. 1-19
Author(s):  
F. P. Campos ◽  
P. Sarmento ◽  
W. T. Mattos ◽  
L. Gerdes ◽  
R. A. Carnevalli ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Nutritional Quality ◽  
Block Design ◽  
Production Capacity ◽  
Morphological Characteristics ◽  
Light Interception ◽  
Panicum Maximum ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

Abstract Efforts were made to obtain forage cultivars with high production capacity and quality and to determine the ideal time to start animal grazing, respecting the morphological characteristics of the grasses. For this purpose, the nutritional value, digestibility and characteristics of the forage canopy of Aries and Aruana (Megathyrsus maximus (Jacq.) or Panicum maximum) cultivars kept under rotational stocking with sheep were evaluated and the management height was determined based on 95% light interception (LI). The experiment was evaluated with repeated measures over time (grazing cycles by season, in 2 years) with a treatment factor (grass cultivar) in a completely randomized block design with eight replicates, using 800 m2 paddocks. The height of the forage canopy before grazing (pre-grazing) determined by 95% LI stabilized around 29 cm. The highest proportion of Aruana leaf blades was observed in winter. Aries grass presented similar leaf blade percentages in summer, autumn and spring. Similar forage accumulation (FA) was observed for both cultivars, decreasing in spring, autumn and winter. Higher crude protein and in vitro dry matter digestibility (IVDMD) were seen in Aries grass, while Aruana grass presented higher neutral and acid detergent fibre and cellulose and lower IVDMD. The nutritional quality of the grasses was maintained throughout the year. The height of the forage canopy of Aries and Aruana recommended for entry into the paddock is 29 cm to maintain nutritional quality. The successful management strategy adopted for grasses combined with nitrogen fertilization provided a greater forage mass quality.

scholarly journals Nutritional value of sorghum silages

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Significance Level ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
Nutritional Characteristics ◽  
High Participation ◽  
Sorghum Genotypes

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Response of growth and yield of two soybean varieties to seed pre-treatment applications

2021 ◽  
Vol 883 (1) ◽  
pp. 012052
Author(s):  
N Herawati ◽  
A R Aisah ◽  
I Mardian ◽  
B N Hidayah ◽  
B T R Erawati
Keyword(s):  
Plant Height ◽  
Seed Treatment ◽  
Seed Quality ◽  
Block Design ◽  
Growth And Yield ◽  
Agronomic Crops ◽  
Randomized Block Design ◽  
Two Factors ◽  
Pre Treatment ◽  
Four Levels

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita
Keyword(s):  
Resistant Starch ◽  
Room Temperature ◽  
Potato Starch ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cooling Methods ◽  
Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

scholarly journals PSIV-3 Effect of winter-feeding system and roughage level in feedlot ration on subsequent spring grazing behavior and dry matter intake of beef growing heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 228-229
Author(s):  
María Victoria Burjel ◽  
Alvaro Simeone ◽  
Oscar Bentancur ◽  
Natalia Zabalveytia ◽  
Virginia Beretta
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Block Design ◽  
Ingestive Behavior ◽  
Dry Matter Intake ◽  
Feeding System ◽  
Grazing Behavior ◽  
Randomized Block Design ◽  
Winter Feeding

Abstract This study aimed to evaluate the effect of winter-feeding system (grazing, GFS vs. feedlot, FFS) and ration roughage level (RL), on subsequent spring grazing behavior and forage dry matter intake (DMI). Thirty-two Hereford female calves (149 ± 13 kg) were assigned to four winter dietary treatments (110 days) in a randomized block design (n = 2/treatment): three feedlot rations differing RL (Setaria italica) (0%, 35% or 70% DM) or oats grazing (5 kg DM/100 kg LW). In spring (84 days) all treatments grazed a mixed grass-legume pastures (4317 ± 1009 kg DM/ha; 6 kg DM/100 kg LW). During weeks 1, 3 and 5 after changing diets, diurnal behavior activity (effective/search grazing, rumination, idling or water intake) was visually recorded and DMI estimated from the in vivo DM digestibility (DMD) and fecal production. Data were analyzed according to a block design with repeated measures, with general model including block, treatment (T), week (W) and T×W effects. Means were compared through orthogonal contrasts (Table 1). Spring DMI varied with T (P = 0.0084) regardless of W effect (P = 0.1619), with higher values for GFS compared to FFS (1.55 vs. 1.16 kg/100 kg LW, P = 0.0034) and for 70RL compared to 35RL (P = 0.0286). DMD did not differ between treatments (P = 0.2488) but was lower on W1 than W5 (55 vs. 64 %, P = 0.0150). T×W was significant (P &lt; 0.0001) for effective grazing, rumination and idling. On W1, heifers from GFS grazed less (0.40 vs. 0.49, P = 0.0268) but tended to ruminate more (0.26 vs. 0.18, P = 0.0591) than FFS. As pasture DMD improved (W5) GFS grazed more (0.64 vs. 0.51, P = 0.0020) but ruminated less (0.14 vs. 0.21, P = 0.0450) than FFS. Transition from FFS to spring grazing might reduce pasture DMI modifying animal ingestive behavior compare to animals from GFS. Impact could be higher when ration RL is below 70%.

scholarly journals PSIV-10 Preference evaluation of plant functional types grasses in calves

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 225-226
Author(s):  
María A Vulliez ◽  
María Bruni ◽  
Ana I Trujillo ◽  
Martín Durante ◽  
Lucrecia Lezana ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Fixed Effects ◽  
Mixed Model ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Functional Types ◽  
Randomized Block Design ◽  
Functional Types Of Plants ◽  
Group B ◽  
Cut Leaves

Abstract The functional types of plants (PFT) ecological approach could be used to link the preferences of ruminants. In order to investigate if ruminant preferences were associated with this approach, three PFT groups were evaluated. A group “B”, represented by Paspalum urvillei (PU-B), “C” by Nassella neesiana (NN-C) and Sporobolus indicus (SI-C) and “D” by Andropogon lateralis (AL-D). In a randomized block design with repeated measures over time, two independent cafeteria trials (autumn-winter, A-W, and spring-summer, S-S) were carried out in calves (n = 8 for A-W; n = 4 for S-S; BW=240–270 kg) arranged in four pens. In three sessions of ten minutes each, calves received 120–160 gDM of fresh cut leaves from the different species. Dry matter intake (DMI), nitrogen intake (NI) and neutral detergent fiber intake (NDFI) were measured. Data were analyzed with a mixed model that included specie and session as fixed effects and blocks as a random. The statistical analyses were performed with SAS (University Edition), data is expressed as lsmeans and was considered to differ when Tukey test presented P &lt; 0.05. No significant differences were observed between sessions. The DMI and NI were higher in NN-C and lower in AL-D (P &lt; 0.01) during A-W. However, in S-S, higher DMI and NI were observed in AL-D and SI-C while NN-C presented the lower DMI and NI (P &lt; 0.01). The FDNI did not show differences in A-W meanwhile in S-S was positively associated with DMI (Table1). Interestingly, when the season changed, it was observed a change in PFT preference, in A-W calves preferred NN-C whereas in S-S preferred AL-D. However, attributes associated with preferences were maintained in both stations, for instance, higher DMI was associated with higher NI. Additional research is required on the interactions between PFT approach and the preference of animals for different species.

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