Antibacterial activity of ultrasonic extracts of Salvia stepposa growing in Kazakhstan

This article presents data on the studies on antibacterial activity of ultrasonic extracts of Salvia stepposa (steppe sage) growing wild on the territory of the Republic of Kazakhstan. Screening for the antibacterial activity of the presented samples was carried out by the disc-diffusion method against strains of gram-positive bacteria Staphylococus aureus, Bacillus subtilis, gram-negative strains Escherichia coli and the yeast Candida albicans. The results showed that in relation to Staphylococus aureus the most pronounced antibacterial activity with growth inhibition zones 35 ± 1 mm is possessed by 30 % ultrasonic extract of steppe sage flowers, in relation to Bacillus subtilis 49 ± 1 mm — 40 % ultrasonic extract of steppe sage leaves, for Escherichia coli 24 ± 1 mm — 70 % ultrasonic extract of steppe sage leaves. This study demonstrated that, 30 % ultrasonic extract of flowers and 90 % ultrasonic extract of flowers and leaves of steppe sage showed weak activity against yeast fungus Candida albicans with growth inhibition zones 12 ± 1 mm, 12 ± 1 mm and 11 mm, respectively. As a result, out of 8 extracts isolated by ultrasonic activation only 2 extracts showed antibacterial activity against 4 studied bacteria. Additionally, it was found that the use of ultrasonic extraction can reduce the extraction time of biologically active substances in 8–9 times compared to conventional extraction methods.

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Synthesis and Antibacterial Activities of N-Phenylpyrazolines from Veratraldehyde

Materials Science Forum ◽

10.4028/www.scientific.net/msf.901.124 ◽

2017 ◽

Vol 901 ◽

pp. 124-132

Author(s):

Artania Adnin Tri Suma ◽

Tutik Dwi Wahyuningsih ◽

Deni Pranowo

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Bacillus Cereus ◽

Shigella Flexneri ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Diffusion Method ◽

Agar Well Diffusion Method

Some novel N-phenylpyrazolines were synthesized and investigated for their antibacterial activitiy. Chalcones 2-4 which were prepared from acetophenone and veratraldehyde derivatives were reacted with phenylhydrazine to give N-phenylpyrazolines 5-7. All of the synthesized compounds were characterized using FTIR, GC-MS, and NMR spectrometers. Further, antibacterial activity of N-phenylpyrazolines were evaluated by agar well-diffusion method against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Escherichia coli, and Shigella flexneri. The highest activity (highest inhibition zone) of compound 5 was 2.6 mm (at 1000 ppm) against B. subtillis, compound 6 was 7.25 mm (at 1000 ppm) against S. aureus, and compound 7 was 6.75 mm (at 500 ppm) against S. aureus. The results indicated that compound 6 and 7 exhibited promising antibacterial activity.

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Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

Journal of Bangladesh College of Physicians and Surgeons ◽

10.3329/jbcps.v33i3.28055 ◽

2016 ◽

Vol 33 (3) ◽

pp. 140-145

Author(s):

Mst Laila Akter Banu ◽

AKM Bashar ◽

Md Mujibur Rahman Howlader ◽

Md Shamsul Alam ◽

Md Ashraf Hussain

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Antimicrobial Efficacy ◽

Diffusion Method ◽

Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Students t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

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Antibacterial activity of cis- and trans-resveratrol isolated from Polygonum cuspidatum rhizome

Acta periodica technologica ◽

10.2298/apt0637131k ◽

2006 ◽

pp. 131-136 ◽

Cited By ~ 3

Author(s):

Zoran Kukric ◽

Ljiljana Topalic-Trivunovic

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Antibacterial Effect ◽

Disk Diffusion ◽

Diffusion Method ◽

Polygonum Cuspidatum ◽

Disk Diffusion Method ◽

Cis And Trans

The work is concerned with the antibacterial effect of ethanolic solutions of cis- and trans-resveralrol (cis-.lrans-3,5.4'-trihydroxystilbene) obtained by the extraction (ethanol-water 1:1 v/v) of Polygonum cuspidatum rhizome. Antibacterial activity was tested by disk diffusion method on the following bacteria: Escherichia coli, Sarcina liitea. Bacillus subtilis and Staphylococcits sp., using extract concentrations of 5 mg/disk. All tests showed significant antimicrobial activity, whereby the extract with trans-resveratrol exhibited more significant effect than the extract of cis-resveratrol.

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Antibacterial Activity of Beluntas (Pluchea indica L.) Leaves Extract using Different Extraction Methods

Jurnal Riset Biologi dan Aplikasinya ◽

10.26740/jrba.v2n2.p49-54 ◽

2020 ◽

Vol 2 (2) ◽

pp. 49

Author(s):

Kinanti Ayu Puji Lestari ◽

Putri Priliawati Pranoto ◽

Sofiyah Sofiyah ◽

Majidah Musyirah ◽

Faizatin Isnaini Pratiwi

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Digestive Tract ◽

Antibacterial Agent ◽

Extraction Method ◽

Extraction Methods ◽

Antibacterial Compounds ◽

The Public ◽

Tract Infections

Escherichia coli and Bacillus subtilis are bacteria that cause disease in the digestive tract. Pluchea indica L. had the pharmacological activity of antiseptic power against bacteria that cause digestive tract infections because of contained antibacterial compounds. The study on the comparison of extraction methods will enable the public to choose a better extraction method to use as an antibacterial agent. This study aimed to identify the content of antibacterial compounds in Pluche indica L. and to determine the antibacterial ability of Pluche indica L. extract using maceration, percolation, and soxhletation methods. The results showed that Pluchea indica L. contains flavonoids, alkaloids, saponins, and tannins. The highest diameter inhibition of Escherichia coli and Bacillus subtilis was obtained from Pluchea indica L. leaves extract extracted by using the Soxhletation method.

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Chemical Composition and Antimicrobial Activity of Subcritical CO2 Extract of Lepidium latifolium L. (Brassicaceae)

International Journal of Biomaterials ◽

10.1155/2021/4389967 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Balzhan B. Azimkhanova ◽

Gulbaram O. Ustenova ◽

Kamalidin O. Sharipov ◽

Kayrolla D. Rakhimov ◽

Galiya M. Sayakova ◽

...

Keyword(s):

Escherichia Coli ◽

Carbon Dioxide ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Biologically Active ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Lepidium Latifolium ◽

Carbon Dioxide Extraction

The genus Lepidium L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties (L. crassifolium Waldst. et Kit., L. perfoliatum L., L. ruderale L., and L. latifolium L.), used in folk medicine as means of antibacterial, irritant, laxative, antitumor, analgesic, and anthelmintic action. Methods. Raw materials were collected from Almaty region (Republic of Kazakhstan). Lepidium latifolium L. herb’s carbon dioxide extract (CO2 extract) was obtained by subcritical carbon dioxide extraction. A gas chromatograph with a mass spectrometric detector was used to determine the component composition of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilution and the disc-diffusion method. Four microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, Klebsiella pneumonia ATCC 10031, and Candida albicans ATCC 10231. Results. The technology of carbon dioxide extraction has undoubted advantages over traditional methods of extraction: it has a controlled selectivity in relation to groups of biologically active substances, allows deep extraction, and maximizes the release of rich complexes of compounds contained in plants. In this study, firstly, the CO2 extract was obtained under subcritical conditions from the aerial part of L. latifolium L., and the composition was determined. Hexane was the best solvent for CO2 extract, and 40 components were identified. Screening of antimicrobial activity of the L. latifolium’s CO2 extract showed the essential activity of all clinically significant strains tested: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Candida albicans. Conclusions. This research showed that the CO2 extract of the raw material of Lepidium latifolium L. contains biologically active compounds exhibiting an essential antimicrobial effect, and therefore it is possible to recommend for the development of various drugs for use in medical practice.

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The Antibacterial Activity of the Extracts of Apocynum Venetum and Apocynum Venetum Fiber

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.233-235.2328 ◽

2011 ◽

Vol 233-235 ◽

pp. 2328-2331 ◽

Cited By ~ 1

Author(s):

Hui Juan Wang ◽

Hao Chen ◽

Guang Ting Han

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Ethyl Acetate ◽

Antibacterial Effect ◽

Antibacterial Activities ◽

Butyl Alcohol ◽

Apocynum Venetum

In this study, the total flavone contents of Apocynum venetum extract and Apocynum venetum fiber extracts were evaluated. Their antibacterial activity was tested via testing the antibacterial effect of their aqueous, ethyl acetate and n-butyl alcohol extracts. The results were showed that both the materials extracts at the concentration of 100, 50mg/ml had significantly antibacterial activities against staphylococcus aureus, and had a few effect on bacillus subtilis, pseudomonadaceae, pseudomonas aeruginosa, staphylococcus epidermidis, escherichia coli and candida albicans.

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The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v2i2.9781 ◽

2008 ◽

Vol 2 (2) ◽

Author(s):

Nurliana Nurliana ◽

Mirnawati Sudarwanto ◽

Lisdar I. Sudirman ◽

A. W. Sanjaya

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Methanol Extract ◽

Diffusion Method ◽

Traditional Food ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Agar Disc ◽

Inhibition Zones ◽

Initial Research

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.

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Evaluation of Antibacterial and Antifungal Activity of Herbs Used in Treatment of Diabetes, Malaria and Pneumonia in Kisii and Nyamira Counties Region, Kenya

Journal Of Tropical Pharmacy And Chemistry ◽

10.25026/jtpc.v5i4.314 ◽

2021 ◽

Vol 5 (4) ◽

pp. 330-337

Author(s):

Moses A. Guto Maobe ◽

Leonard Gitu ◽

Erastus Gatebe

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Diffusion Method ◽

Root Extract ◽

Plant Parts ◽

Toddalia Asiatica ◽

Antibacterial And Antifungal

The herbs Carissa spinarum, Physalis minima and Toddalia asiatica have traditionally been used in healing diabetes, malaria and pneumonia by the communities around the Kisii region, Kenya. However in the available literature, there is scanty information on effectiveness of different plant parts of the herbs in healing the ailments. The objective of this study was to investigate the potential antimicrobial and antifungal activity of methanolic extract of whole plant Physalis minima, leaf and root of Carissa spinarum and Toddalia asiatica against gram positive bacteria Staphylococcus aureus (ATCC 25923), gram negative bacteria Escherichia coli (ATCC 25922) and fungus Candida albicans (ATCC14053). Antibiotic disc methicillin, cotrimoxazole, chloramphenicol, gentamicin, ampicillin, nalidixic and nitrofurantoin were used in the study. In each herb, plant part was extracted by soaking in methanol/dichloromethane in ratio 1:1 for a week, filtered, concentrated by rotary vapor and cooled. The same process was repeated three times for all samples. The study was conducted by agar well diffusion method. Methanolic root extract of Toddalia asiatica showed highest antibacterial activity against Staphylococcus aureus (ATCC 25923), root extract of Carissa spinarum had highest antibacterial activity against Escherichia coli (ATCC 25922) while root extract of Toddalia asiatica showed highest antifungal activity. It was concluded that root extract of Toddalia asiatica showed highest antibacterial activity 16.7mm against Staphylococcus aureus(ATCC 25923), root extract of Carissa spinarum had highest antibacterial activity 10 mm against Escherichia coli (ATCC 25922) while root extract of Toddalia asiatica had highest antifungal activity 18 mm against Candida albicans.

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UJI ANTIBAKTERI RIMPANG GANDASULI (HEDYCHIUM CORONARIUM) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI DENGAN PERBANDINGAN METODE EKSTRAKSI

Journal of Pharmacopolium ◽

10.36465/jop.v1i2.326 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Aris Suhardiman

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Sulfuric Acid ◽

Acid Reflux ◽

Diffusion Method ◽

Hedychium Coronarium ◽

Antibacterial Compound ◽

Inhibition Zones ◽

Positive Effect

Indonesia has various types of plants those have potential as medicine to overcome health problems in line with the expanding of traditional medicine industry. One example of plants from the Zingiberaceae family is Hedychium coronarium, which is not widely known by the community despite it has efficacy as an antibacterial which is not inferior to other Zingiberaceae family. The research was conducted to determine whether the Hedychium coronarium rhizome shown to have antibacterial activity against the growth of Staphylococcus aureus and Escherichia coli, knowing antibacterial compound that contained in Hedychium coronarium rhizome, also knowing the most effective extraction method to take the antibacterial compound from Hedychium coronarium rhizome. The gandasuli rhizome was extracted by maceration and reflux with 96% ethanol. Antibacterial activity test carried out by the agar diffusion method, and the analysis of antibacterial compounds with bioautography, and the results are monitored with vanillin-sulfuric acid. Maceration and reflux extract at a concentration of 90% has antibacterial activity with inhibition zones, each for 8 and 10 mm against Escherichia coli as well as 9 and 10 mm against Staphylococcus aureus. Bioautography results show a positive effect on the appearance of vanillin-sulfuric acid. Reflux extract has better antibacterial activity, while the antibacterial compound is an essential oil group. Keywords: Hedychium coronarium, antibacterial, Staphylococcus aureus, Escherichia coli, bioautography.

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Chemical Profile, in vitro Antibacterial and Antioxidant Activities ofJuniperus phoeniceaL. and Cotula cinerea (Del.) Essential Oils from Southwestern Algeria

Phytothérapie ◽

10.3166/phyto-2018-0061 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S74-S83 ◽

Cited By ~ 1

Author(s):

D. Ghouti ◽

H.A. Lazouni ◽

A. Moussaoui ◽

D. Chabane Sari

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antioxidant Activities ◽

Diffusion Method ◽

Juniperus Phoenicea ◽

Cotula Cinerea

This work aims to study the chemical composition, antimicrobial and antioxidant activities ofJuniperus phoeniceaL. andCotula cinerea(Del.) essential oils from southwestern Algeria. The hydrodistilled oils obtained were characterized by gas chromatography–mass spectrometry (GC– MS). A total of 45 and 25 compounds were identified representing 100% ofJuniperus phoeniceaL. andCotula cinerea(Del.) respectively. The dominant component was α-pinene (46.437%) forJuniperus phoeniceaL. and α-thujone (32.35%) forCotula cinerea(Del.). The in vitro antibacterial activity of the essential oils was evaluated against nine foodborne pathogenic bacteria and one yeast, Candida albicans, using the disk diffusion method; the minimum inhibitory concentration (MIC) values were examined (micro dilution). The results suggest that the essential oil ofJuniperus phoeniceahas a significant to moderate antibacterial activity on all strains of bacteria. The most sensitive strains were Bacillus cereus, Bacillus subtilis,Micrococcus luteus,Pseudomonas aeruginosa, andCandida albicanswith (MIC = 0.04 to 0.63 mg/ml).Cotula cinereaoil showed moderate to low sensitivity to the test strains. Its best activity was against Bacillus subtilis (MIC = 0.303 mg/ml). The DPPH test conducted to evaluate the antioxidant activity of both oils showed a good to moderate capacity forJuniperus phoeniceaL. (concentration of an inhibitor when the response is reduced by half, IC50 = 0.76 mg/ml) andCotula cinerea(Del.) [IC50 = 28. mg/ml] oils respectively. The promising bioactivities of these plants suggest that they may be a new source of preservative molecules.

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