scholarly journals PHYTOCHEMICAL ANALYSIS OF Turnera diffusa WILLD

2020 ◽  
Vol 8 (11) ◽  
pp. 402-411
Author(s):  
Maria Regina de Oliveira Silva ◽  
Maria Herbênia Lima Cruz Santos ◽  
Xirley Pereira Nunes ◽  
Emanuel Ernesto Fernandes Santos ◽  
Eugênio Bispo da Silva Júnior
Keyword(s):  
Secondary Metabolites ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
Phytochemical Screening ◽  
Turnera Diffusa ◽  
Pharmacological Potential ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Chemical Class

Turnera diffusa WILLD is a plant used in folk medicine as a natural stimulant, and the objective of this research is to perform the phytochemical analysis of the secondary metabolites of the damiana of occurrence in the Juazeiro region, northern Bahia - Brazil. A phytochemical screening was performed from three Crude Ethanol Extracts (BSE), EEB1: leaves and flowers; EEB2: stems and EEB3: root. A thin analytical layer chromatography was performed using specific developers to detect each chemical class. Alkaloids, coumarins, anthranic derivatives, phenolic compounds, mono, sequi and diterpenes, naphthoquinones, triterpenes and steroids, saponins, hydrolyzed tannins and xanthines were identified in the three extracts. Only in the BSE of leaves and flowers observed the presence of antroquinones and aglycones. The secondary metabolites identified in the study evidence the pharmacological potential of Turnera diffusa. Phytochemical screening concluded the presence of fourteen classes of secondary metabolites in damiana, indicating important medicinal potential with pharmacological effects reported in the literature.

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM HOLOPTELEA INTEGRIFOLIA (ROXB.) PLANT LEAVES USING HPTLC

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (09) ◽  
pp. 67-71
Author(s):  
R. C. Sutar ◽  
◽  
D. S Musmade
Keyword(s):  
High Performance ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The present study was conducted to identify the phenols from methanol extracts (MHI) of medicinally and economically useful leaves of Holoptelea integrifolia (Roxb.) plant using High Performance Thin Layer Chromatography (HPLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out on CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp. Preliminary phytochemical screening of methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins, phenols and carbohydrates. HPT LC finger printing of phenols of methanolic extract of leaf revealed seven polyvalent phytoconstituents (7 peaks) and corresponding ascending order of Rf values in the range of 0.15 to 0.75. From the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phenols in methanol extracts.

scholarly journals Phytochemical Screening and TLC Profiling of Various Extracts of Reinwardtia indica

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Mehta Sonam ◽  
Rana Pawan Singh ◽  
Pooja Saklani
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Scientific Basis ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Biochemical Compound ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
The Himalaya

Reinwardtia indica, belongs to family Linaceae known as yellow flax or pyoli commonly found in the Himalaya. The plant has varied ethno medicinal importance such as aerial parts are used to prevent bleeding of cuts and as mouthwash; leaves are used in the treatment of paralysis and as natural antibiotic. Qualitative phytochemical screening of chloroform, acetone, ethanol, methanol and aqueous extracts was performed to explore scientific basis of ethno medicinal potential. It confirmed the presence of many phytochemicals like alkaloids, flavanoids, phenols, tannins, saponins, terpenoids, phlobatanins etc. in various extracts. Most of the phytochemicals were found in methanol and ethanol extracts. Thin Layer chromatography (TLC) of the acetone, methanol, chloroform and aqueous extract was performed for four important phytochemicals alkaloids, flavanoids, tannins and phenol. Flavanoids showed their presence in all extracts with one spot in each (Rf 0.8 for acetone, 0.918 for methanol, 0.816 for chloroform and 0.737 for aqueous extract). Alkaloids and tannins were found in acetone and methanol extract while phenol was present only in methanol extract (Rf 0.8). These findings provided the evidence that Reinwardtia indica is a potent source for some medicinally important phytochemicals and it justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active biochemical compound of medicinal utilities

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

TELAAH FITOKIMIA KULIT KACANG TANAH (Arachis hypogaea, L).

2021 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Rizki Nisfi Ramdhini ◽  
Isna Mulyani ◽  
Syaikhul Aziz
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Waste Product ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Processing Industry ◽  
Arachis Hypogaea L ◽  
Tlc Analysis ◽  
Layer Chromatography

Peanut peel are a waste product of the peanut processing industry with little commercial value. Some of studies have been conducted indicating peanut peel can be beneficial as a source for traditional medicinal products since it is also rich of antioxidants. The aim of this research was to identify the content of secondary metabolites on the peanut peel. The method used was maseration with 96% ethanol. Phytochemical screening and assaying were performed using thin layer chromatography (TLC) method. The results of TLC analysis showed that the secondary metabolites in peanut peel were positive for flavonoids, alkaloids, tannins and quinon. Keywords: Peanut peel, Phytochemical, Thin-Layer chromatography (TLC)

scholarly journals TABLET FORMULATION FROM MENIRAN (PHYLLANTHUS NIRURI L.) EXTRACT WITH DIRECT COMPRESSION METHOD

2018 ◽  
Vol 10 (4) ◽  
pp. 98
Author(s):  
Resmi Mustarichie ◽  
Dradjad Priambodo
Keyword(s):  
Secondary Metabolites ◽  
Pharmaceutical Preparation ◽  
Magnesium Stearate ◽  
Phytochemical Screening ◽  
Compression Method ◽  
Phyllanthus Niruri ◽  
Disintegration Time ◽  
Quality Testing ◽  
Layer Chromatography

Objective: The aim of the present study was to obtain an optimized formula of meniran (Phyllanthus niruri L.) extract tablets that fulfilled the requirements as a good pharmaceutical preparation based on Indonesian Pharmacopoeia IV and USP XVII.Methods: P. niruri plant was collected and determined at the Laboratory of Plant Taxonomy, Universitas Padjadjaran. First performed phytochemical screening to determine the content of secondary metabolites. Then designed five kinds of tablet formulas of P. niruri extract using a direct compressed method with a variation of concentration of filler. Each formula contains a similar concentration of P. niruri extract as the active ingredient, avicel PH 102 and amprotab with varying concentrations as filler, talcum, and magnesium stearate as a lubricant and Aerosil®200 vv as an adsorbent. Tablet print mass and quality of the resulting tablets were then evaluated. Also, check whether the resulting tablets still contain P. niruri extract as the active substance or not.Results: The results of phytochemical screening of simplicia and viscous plant extract showed the presence of alkaloids, polyphenols, tannins, and flavonoids as secondary metabolites. The five formulas made contain avicel PH 102 and amprotab as binders and crushers and the expected results such as shape and durability as desired. The results of examination of shrinkage rate of mass drying of tablet prints from the above five formulas indicated an increase of value from formula A (5.4609 %) to formula E (5.8600%). This was because avicel PH 102 and amprotab had a considerable moisture content, so with the combination of both fillers could increase the water content from mass print tablets. Real density, compact density, and true density decreased from formula A to formula E. The amount of these densities were influenced by the shape and size of the particles. Flowability increased from formula A (23.7124°) to formula E (26.4210°) whereas compressibility increased from formula A (21.7222%) to formula E (29,4121%). Flowability and compressibility increase might be due to the uniformity of the particle size between the amprotabs and the other additives which could cause electrical charges to the print mass affecting the speed and flow of the print mass. All quality testing results including Weight (mg), Thickness (mm), Diameter (mm), Hardness (N), Friability (%) and Disintegration time (min.) had met the requirements. Thin Layer Chromatography showed that the resulting tablets still contain P. niruri extract as the active substance.Conclusion:. Overall results showed that the formulation fulfilled the requirements as a good pharmaceutical preparation based on Indonesian Pharmacopoeia IV and USP XVII.

scholarly journals Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
ELISA HERAWATI ◽  
RICO RAMADHAN ◽  
FARIDA ARIYANI ◽  
MARJENAH MARJENAH ◽  
IRAWAN WIJAYA KUSUMA ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Phytochemical Screening ◽  
Wild Mushrooms ◽  
Tropical Regions ◽  
Color Changes ◽  
Ic50 Value ◽  
In The Wild

Abstract. Herawati E, Ramadhan R, Ariyani F, Marjenah, Kusuma IW, Suwinarti W, Mardji D, Amirta R, Arung ET. 2021. Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions. Biodiversitas 22: 4716-4721. This study aims to determine the presence of secondary metabolites which generally have bioactivity that is antioxidant with phytochemical tests and antioxidant tests in Auricularia auricula, Schyzophyllum commune, Microporus xanthopus, and Trametes versicolor fungi that grow in the wild. Phytochemical analysis was carried out by testing color changes and modifications, while antioxidant tests were carried out by using a UV-Vis spectrophotometer and a DPPH solution (1,1-diphenyl-2-picrylhydrazyl). This research found that edible wood fungus that grows, wild such as A. auricula, contains secondary metabolites, namely flavonoids, triterpenoids, saponins, and tannins, but does not contain steroids, alkaloids, carotenoids, or coumarin, while S. commune contains secondary metabolites, namely flavonoids, steroids, tannins, and coumarin, but does not contain triterpenoids, alkaloids, carotenoids, or saponins and non-edible wood fungus, such as M. xanthopus, contains secondary metabolites, namely, flavonoids, saponins, tannins, coumarin, and steroids, but does not contain triterpenoids, alkaloids, or carotenoids, while T. versicolor contains compounds, secondary metabolites, flavonoids, saponin, tannins, coumarin, and triterpenoids, but does not contain steroids, alkaloids, or carotenoids. The antioxidant test results are based on IC50 calculations; it is known that the IC50 value of A. auricula is 499.25 g/mL, while S. commune is 121.37 g/mL, M. xanthopus is 251.20 g/mL, and T. versicolor is 493.04 g/mL. Based on the research results, edible and inedible wood mushroom extracts contain several phytochemicals and show potential for antioxidant activity.

scholarly journals PHYTOCHEMICAL SCREENING OF SIJUKKOT EKSTRACT (Lactuca Indica L.)

2020 ◽  
Vol 3 (2) ◽  
pp. 53
Author(s):  
Jesica C.R. Panjaitan ◽  
Albinus Silalahi
Keyword(s):  
Secondary Metabolites ◽  
Test Results ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Rotary Evaporator ◽  
Lactuca Indica ◽  
Ethanol Extracts ◽  
The Village

Have been carried out Phytochemical screening on Sijukkot  which determited as Lactuca indica L, the plant from Gibeon hill forrest area in the village of Parsaora Sibisa Ajibata, Toba Samosir, North Sumatera. Test carried out to determine the composition of secondary metabolites contained in these plants. It was started by extracting plant leaves using 96% ethanol solvent which was carried out by maceration method and concentrated using a rotary evaporator. The concentrated extract obtained was sponsored by phytochemical tests. Phytochemical test results on ethanol extracts from the leaves of the Sijukkot plant were obtained compositions namely Flavonoids, tannins, saponins, steroids and triterpenoids.

scholarly journals Secondary metabolites in plants: main classes, phytochemical analysis and pharmacological activities

Bionatura ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 1000-1009
Author(s):  
Irina Francesca González Mera ◽  
Daniela Estefanía González Falconí ◽  
Vivian Morera Córdova
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolism ◽  
Chemical Compounds ◽  
Biological Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Functions ◽  
Pharmacological Activities

Plants are an essential source of chemical compounds with different biological properties that man can use to his advantage. These substances are mainly produced as a result of chemical conversions of secondary metabolism. This article reviews the main classes of secondary metabolites that synthesize plants as well as their characteristics and their biological functions. Examples are provided for each of the classes. Emphasis is placed on the methods of extracting secondary metabolites and phytochemical screening, as well as on the main pharmacological activities described for the MS.

FINGER PRINTING ANALYSIS OF THE PHENOLS FROM PERGULARIA DAEMIA FORSK PLANT LEAVES AND STEM USING HPTLC

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 32-36
Author(s):  
N G Sutar ◽  
Keyword(s):  
High Performance ◽  
Research Study ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Plant Leaves ◽  
Methanol Extracts ◽  
Finger Printing ◽  
Rf Values ◽  
Layer Chromatography ◽  
Tungsten Lamp

The research study was carried out to identify the phenols from methanol extracts (MPD) of medicinally and efficiently useful leaves and stem of Pergularia daemia (Forsk) plant using High Performance Thin Layer Chromatography (HPTLC) technique. Preliminary phytochemical screening was done and HPTLC studies were carried out. Densitometry scanning was performed in the reflectance absorbance mode at 540 nm and operated by Win CATS software with the help of tungsten lamp. Preliminary phytochemical screening of methanol extract of P. daemia shows the presence of alkaloids, glycosides, flavonoids, phenols and carbohydrates. HPTLC finger printing of phenols of methanolic extract of leaf and stem revealed eleven polyvalent phytoconstituents (13 and 10 peaks) and corresponding ascending order of Rf values in the range from 0.08 to 0.65 in leaf and 0.02 to 0.66 in the stem. From the result of preliminary phytochemical analysis and above Rf values from HPTLC, we have concluded the presence of phenols in methanol extracts.

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