scholarly journals THE INFLUENCE OF CRYOCORSERVED PLACENTA ON THE DYNAMICS OF CHANGES IN CLINICAL INDICATORS IN THE TREATMENT OF PATIENTS WITH PHLEGMONS OF JAW-FACIAL LOCALITIS

2021 ◽  
pp. 11-14
Author(s):  
V.O. Lychman ◽  
K.P. Lokes ◽  
D.S. Avetikov ◽  
N.V. Gorlach ◽  
O.O. Goncharenko ◽  
...  
Keyword(s):  
Circadian Rhythms ◽  
Inflammatory Process ◽  
Inflammatory Diseases ◽  
Key Factors ◽  
Operating Period ◽  
Clinical Indicators ◽  
Environmental Signals ◽  
Standardized Protocol ◽  
Wide Range ◽  
Purulent Wound

Today, information about circadian rhythms is widespread. Circadian rhythms are generated by clocks that are endogenous in nature and fluctuate even in the absence of environmental signals. Circadian clocks affect a wide range of biological processes, including neuronal, endocrine, metabolic, and behavioral functions. One of the key factors influencing behavioral patterns is the chronotype, which is defined as the human circadian typology and is a behavioral manifestation of the human internal circadian clock system that can be assessed using several methodologies that classify people into morning or evening chronotypes[1]. The aim of our study was to establish how to use a standardized protocol for medical care for odontogenic phlegmons of maxillofacial localization, taking into account the biological rhythm of patients.A total of 30 patients with odontogenic phlegmons of maxillofacial localization (2-3 cell spaces), aged 35 to 60 years, without systemic chronic diseases, regardless of gender, who underwent surgery in the form of opening and drainage of phlegmon with intravenous introduction of cryopreserved placenta.For clinical characterization of the process of purulent wound healing we used the proposed standardization tables of quantitative values of indicators that determine the dynamics of changes in general condition (P-1), the dynamics of local changes in patients with phlegmons of maxillofacial localization (P-2) and the dynamics of changes in a purulent wound (indicator P-3).Analyzing the dynamics of changes in the P-1.1 indicator, it should be emphasized that this indicator reached its maximum values for 1 day after surgery, which is probably due to the body's response to iatrogenic trauma on the background of the inflammatory process. Analyzing the decrease of this indicator during the 3rd and 5th days, it should be noted that its significant value is observed in the period between the 3rd and 5th days closer to the 5th, on average by 1.78 ± 0.24. Analysis of the dynamics of the indicator P-1.2 gives grounds to show its uniform decrease during 1, 3 and 5 days after the operating period with normalization of the indicator for 7 days. The change in the dynamics of the P-1.3 indicator by its significant decrease on the 3rd day in 28% of cases and on the 5th day in 72% of cases with normalization of quantitative data on the 7th day is noteworthy. In the study of masticatory disorders (indicator P-4) should be noted the maximum value of this indicator on the 1st day, which is associated with known complications onthe background of purulent inflammatory process and the presence of additional inflammatory process. A significant decrease in this indicator by 0.31 ± 0.09 points on the 5th day in 62% of cases with partial normalization on the 7th day in 62.3% and full normalization in 37.7% of cases is noteworthy. A similar picture was observed in the study of the dynamics of changes in P-1.5, but in contrast to the previous indicator, normalization on day 7 occurred in 82.9% of cases.In the study of indicators showing the dynamics of local changes deserves special attention indicator P- 2.1, which on the 1st day after the operating period reached maximum values with a gradual decrease throughout the postoperative period, but the largest difference in its reduction by an average of 0.23 ± 00.9 scores were recorded on the 5th day in 56.9% of cases in patients with morning chronotype, operated on in the first half of the day.According to the study, it was found that intravenous cryopreserved placenta have an impact on the course of reparative processes in patients with purulent-inflammatory diseases of the maxillofacial localization, depending on the time of surgery.

scholarly journals Evaluation of dynamics of inflammatory process in patients with flegmones of shell-facial plants

2019 ◽  
Vol 41 part 1 (2) ◽  
pp. 25-28
Author(s):  
V. A. Malanchuk ◽  
A.V. Sidoryako
Keyword(s):  
Inflammatory Process ◽  
Inflammatory Diseases ◽  
Hospital Treatment ◽  
Specific Patient ◽  
Regional Administration ◽  
Process Dynamics ◽  
Group I ◽  
Life Threatening ◽  
Purulent Wound ◽  
Clinical Measurements

Modern and glance at the methods of diagnosis and treatment of patients with odontogenic inflammatory diseases of the maxillofacial area and neck was and remains one of the pressing problems that are frequent complications that lead to disorders in the tooth-jaw system, and can be a direct life-threatening ill. Purpose of the study. To increase the effectiveness of treatment of phlegmon of maxillofacial area and neck due to the active evaluation of the dynamics of the inflammatory process comparing a specific patient with standard indicatorschanges in the limits of edema of infiltration. Materials and methods. We carried out diagnostics and complex treatment of 120 patients with odontogenic phlegmons, who were in hospital treatment. The patients were divided into 2 groups: group I – 60 patients, treatment of which was carried out by the traditional method. Group II – 60 patients, modern treatment methods regional administration of attibiotics, vacuum drainage of purulent wound and plasmolifting at the stage of reparation, were added to their standard therapy. Conclusions: The proposed method is simple, reliable and does not require expensive equipment and makes it possible to predict the development of the disease only by clinical measurements of the volume of inflammation. Keywords: maxillofacial area, phlegmon, autoplasma, platelets, evaluation of the inflammatory process dynamics.

Heparin and proteoglycans as anti-inflammatory drugs

1996 ◽  
Vol 16 (01) ◽  
pp. 56-59
Author(s):  
D. J. Tyrrell ◽  
C. P. Page
Keyword(s):  
Inflammatory Diseases ◽  
Therapeutic Applications ◽  
Wide Range ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

SummaryEvidence continues to accumulate that the pleiotropic nature of heparin (beyond its anticoagulant potency) includes anti-inflammatory activities at a number of levels. It is clear that drugs exploiting these anti-inflammatory activities of heparin may offer exciting new therapeutic applications to the treatment of a wide range of inflammatory diseases.

OCCURRENCE OF THE RISKS FOR DEVELOPMENT OF RESPIRATORY DISEASES IN CHILDREN EXPOSED TO THE CHEMICAL FACTORS OF THE EXPOSURE OF ALUMINA REFINERY- ASSOCIATED BUSINESS ACTIVITY

2019 ◽  
pp. 42-47 ◽  
Author(s):  
M.A. Zemlianova ◽  
I.V. Tikhonova
Keyword(s):  
Respiratory Tract ◽  
Respiratory Diseases ◽  
Inflammatory Diseases ◽  
Allergic Inflammation ◽  
Respiratory Morbidity ◽  
Upper Respiratory Tract ◽  
Negative Effects ◽  
Wide Range ◽  
Chemical Factors ◽  
Chronic Lymphoproliferative Diseases

Alumina refineries are among the leading sources of atmospheric air pollution with a wide range of pollutants hazardous to human respiratory organs. It is relevant to study and evaluate the occurrence of the risks for development of respiratory diseases in children living in the area affected by the emission components of an alumina refinery. We assessed air quality of the area under observation and comparison according to monitoring observations, risk of non-carcinogenic effects from the respiratory organs. The content of chemicals in the blood and urine adequate to risk factors was quantified. The structure of individual groups of respiratory diseases was analyzed. The causal relationships of violations of laboratory parameters with an increased content of chemicals in biological media were evaluated. It was found that an aerogenic exposure of chemical pollutants is formed on the territory with the production of metallurgical alumina. It determines the risk for development of respiratory diseases, exceeding an acceptable level up to 49.9 times. In the exposed children, the content of manganese, chromium, nickel, copper, xylenes, formaldehyde and aluminum, fluoride ion in the urine was increased to 4.2 times in relation to the indices in the comparison group. A high level of additional respiratory morbidity(1.8 times) was revealed. Chronic lymphoproliferative diseases of the nasopharynx and inflammatory diseases of the upper respiratory tract (up to 6.6 times more often), inflammatory diseases with a predominance of the mechanism of allergic inflammation ( up to 2.1 times more often)are more often detected in the framework of the respiratory diseases. Negative effects on the part of the respiratory system in the form of activation of antioxidant processes, the development of an inflammatory reaction, local, general and specific sensitization of the respiratory tract were established. It confirms the occurrence of the risks for the development of respiratory diseases in children in the exposure area of the chemical factors of alumina refinery-associated economic activity.

scholarly journals A multi-site, year-round turbulence microstructure atlas for the deep perialpine Lake Garda

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Sebastiano Piccolroaz ◽  
Bieito Fernández-Castro ◽  
Marco Toffolon ◽  
Henk A. Dijkstra
Keyword(s):  
Turbulent Mixing ◽  
Meteorological Data ◽  
Time Of Day ◽  
Monthly Basis ◽  
Lake Garda ◽  
Standardized Protocol ◽  
Wide Range ◽  
Buoyancy Driven Convection ◽  
Monitoring Activity ◽  
Additional Monitoring

AbstractA multi-site, year-round dataset comprising a total of 606 high-resolution turbulence microstructure profiles of shear and temperature gradient in the upper 100 m depth is made available for Lake Garda (Italy). Concurrent meteorological data were measured from the fieldwork boat at the location of the turbulence measurements. During the fieldwork campaign (March 2017-June 2018), four different sites were sampled on a monthly basis, following a standardized protocol in terms of time-of-day and locations of the measurements. Additional monitoring activity included a 24-h campaign and sampling at other sites. Turbulence quantities were estimated, quality-checked, and merged with water quality and meteorological data to produce a unique turbulence atlas for a lake. The dataset is open to a wide range of possible applications, including research on the variability of turbulent mixing across seasons and sites (demersal vs pelagic zones) and driven by different factors (lake-valley breezes vs buoyancy-driven convection), validation of hydrodynamic lake models, as well as technical studies on the use of shear and temperature microstructure sensors.

scholarly journals Structural, Evolutionary, and Functional Analysis of the Protein O-Mannosyltransferase Family in Pathogenic Fungi

2021 ◽  
Vol 7 (5) ◽  
pp. 328
Author(s):  
María Dolores Pejenaute-Ochoa ◽  
Carlos Santana-Molina ◽  
Damien P. Devos ◽  
José Ignacio Ibeas ◽  
Alfonso Fernández-Álvarez
Keyword(s):  
Functional Analysis ◽  
Secretory Pathway ◽  
Pathogenic Fungi ◽  
Fungal Pathogenesis ◽  
Post Translational Modification ◽  
Key Factors ◽  
Wide Range ◽  
Single Deletion ◽  
Asymmetrical Impact ◽  
Substrate Proteins

Protein O-mannosyltransferases (Pmts) comprise a group of proteins that add mannoses to substrate proteins at the endoplasmic reticulum. This post-translational modification is important for the faithful transfer of nascent glycoproteins throughout the secretory pathway. Most fungi genomes encode three O-mannosyltransferases, usually named Pmt1, Pmt2, and Pmt4. In pathogenic fungi, Pmts, especially Pmt4, are key factors for virulence. Although the importance of Pmts for fungal pathogenesis is well established in a wide range of pathogens, questions remain regarding certain features of Pmts. For example, why does the single deletion of each pmt gene have an asymmetrical impact on host colonization? Here, we analyse the origin of Pmts in fungi and review the most important phenotypes associated with Pmt mutants in pathogenic fungi. Hence, we highlight the enormous relevance of these glycotransferases for fungal pathogenic development.

scholarly journals Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Peter D. Leitner ◽  
Ilja Vietor ◽  
Lukas A. Huber ◽  
Taras Valovka
Keyword(s):  
Dna Binding ◽  
Rational Design ◽  
Dissociation Constants ◽  
Inflammatory Diseases ◽  
Dimethyl Fumarate ◽  
Bioinformatic Analysis ◽  
Quantitative Detection ◽  
Regulatory Sequences ◽  
Wide Range ◽  
Inhibitory Drug

AbstractThe nuclear factor kappa B (NF-κB) family of dimeric transcription factors regulates a wide range of genes by binding to their specific DNA regulatory sequences. NF-κB is an important therapeutic target linked to a number of cancers as well as autoimmune and inflammatory diseases. Therefore, effective high-throughput methods for the detection of NF-κB DNA binding are essential for studying its transcriptional activity and for inhibitory drug screening. We describe here a novel fluorescence-based assay for quantitative detection of κB consensus double-stranded (ds) DNA binding by measuring the thermal stability of the NF-κB proteins. Specifically, DNA binding proficient NF-κB probes, consisting of the N-terminal p65/RelA (aa 1–306) and p50 (aa 1–367) regions, were designed using bioinformatic analysis of protein hydrophobicity, folding and sequence similarities. By measuring the SYPRO Orange fluorescence during thermal denaturation of the probes, we detected and quantified a shift in the melting temperatures (ΔTm) of p65/RelA and p50 produced by the dsDNA binding. The increase in Tm was proportional to the concentration of dsDNA with apparent dissociation constants (KD) of 2.228 × 10–6 M and 0.794 × 10–6 M, respectively. The use of withaferin A (WFA), dimethyl fumarate (DMF) and p-xyleneselenocyanate (p-XSC) verified the suitability of this assay for measuring dose-dependent antagonistic effects on DNA binding. In addition, the assay can be used to analyse the direct binding of inhibitors and their effects on structural stability of the protein probe. This may facilitate the identification and rational design of new drug candidates interfering with NF-κB functions.

Enrichment of IGF-1R and PPARγ signalling pathways in orbital inflammatory diseases: steps toward understanding pathogenesis

2021 ◽  
pp. bjophthalmol-2020-318330
Author(s):  
Rohan Verma ◽  
Dongseok Choi ◽  
Allison J Chen ◽  
Christina A Harrington ◽  
David J Wilson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Diseases ◽  
Target Therapy ◽  
Signalling Pathways ◽  
Peroxisome Proliferator ◽  
Healthy Controls ◽  
Gene Expressions ◽  
Peroxisome Proliferator Activated Receptor ◽  
Orbital Inflammation ◽  
Wide Range

BackgroundOrbital inflammatory disease (OID) encompasses a wide range of pathology including thyroid-associated orbitopathy (TAO), granulomatosis with polyangiitis (GPA), sarcoidosis and non-specific orbital inflammation (NSOI), accounting for up to 6% of orbital diseases. Understanding the underlying pathophysiology of OID can improve diagnosis and help target therapy.AimsTo test the hypothesis that shared signalling pathways are activated in different forms of OID.MethodsIn this secondary analysis, pathway analysis was performed on the previously reported differentially expressed genes from orbital adipose tissue using patients with OID and healthy controls who were characterised by microarray. For the original publications, tissue specimens were collected from oculoplastic surgeons at 10 international centres representing four countries (USA, Canada, Australia and Saudi Arabia). Diagnoses were independently confirmed by two masked ocular pathologists (DJW, HEG). Gene expression profiling analysis was performed at the Oregon Health & Science University. Eighty-three participants were included: 25 with TAO, 6 with orbital GPA, 7 with orbital sarcoidosis, 25 with NSOI and 20 healthy controls.ResultsAmong the 83 subjects (mean (SD) age, 52.8 (18.3) years; 70% (n=58) female), those with OID demonstrated perturbation of the downstream gene expressions of the IGF-1R (MAPK/RAS/RAF/MEK/ERK and PI3K/Akt/mTOR pathways), peroxisome proliferator-activated receptor-γ (PPARγ), adipocytokine and AMPK signalling pathways compared with healthy controls. Specifically, GPA samples differed from controls in gene expression within the insulin-like growth factor-1 receptor (IGF-1R, PI3K-Akt (p=0.001), RAS (p=0.005)), PPARγ (p=0.002), adipocytokine (p=0.004) or AMPK (p=<0.001) pathways. TAO, sarcoidosis and NSOI samples were also found to have statistically significant differential gene expression in these pathways.ConclusionsAlthough OID includes a heterogenous group of pathologies, TAO, GPA, sarcoidosis and NSOI share enrichment of common gene signalling pathways, namely IGF-1R, PPARγ, adipocytokine and AMPK. Pathway analyses of gene expression suggest that other forms of orbital inflammation in addition to TAO may benefit from blockade of IGF-1R signalling pathways.

scholarly journals An atlas of Caenorhabditis elegans chemoreceptor expression

2017 ◽  
Author(s):  
Berta Vidal ◽  
Ulkar Aghayeva ◽  
Haosheng Sun ◽  
Chen Wang ◽  
Lori Glenwinkel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Functional Analysis ◽  
Nervous System ◽  
Gene Family ◽  
Sensory Neurons ◽  
Developmental Studies ◽  
Reporter Expression ◽  
Environmental Signals ◽  
Functional Studies ◽  
Wide Range

ABSTRACTOne goal of modern day neuroscience is the establishment of molecular maps that assign unique features to individual neuron types. Such maps provide important starting points for neuron classification, for functional analysis and for developmental studies aimed at defining the molecular mechanisms of neuron identity acquisition and neuron identity diversification. In this resource paper, we describe a nervous system-wide map of the potential expression sites of 244 members of the largest gene family in the C. elegans genome, rhodopsin-like (class A) GPCR chemoreceptors, using classic gfp reporter gene technology. We cover representatives of all sequence families of chemoreceptors GPCRs, some of which were previously entirely uncharacterized. Most reporters are expressed in a very restricted number of cells, often just in single cells. We assign GPCR reporter expression to all but two of the 37 sensory neuron classes of the sex-shared, core nervous system. Some sensory neurons express a very small number of receptors, while others, particularly nociceptive neurons, co-express several dozen GPCR reporter genes. GPCR reporters are also expressed in a wide range of inter- and motorneurons, as well as nonneuronal cells, suggesting that GPCRs may constitute receptors not just for environmental signals, but also for internal cues. We observe only one notable, frequent association of coexpression patterns, namely in one nociceptive amphid (ASH) and two nociceptive phasmid sensory neurons (PHA, PHB). We identified GPCRs with sexually dimorphic expression and several GPCR reporters that are expressed in a left/right asymmetric manner. We identified a substantial degree of GPCR expression plasticity; particularly in the context of the environmentally-induced dauer diapause stage when one third of all tested GPCRs alter the cellular specificity of their expression within and outside the nervous system. Intriguingly, in a number of cases, the dauer-specific alterations of GPCR reporter expression in specific neuron classes are maintained during postdauer life and in some case new patterns are induced post-dauer, demonstrating that GPCR gene expression may serve as traits of life history. Taken together, our resource provides an entry point for functional studies and also offers a host of molecular markers for studying molecular patterning and plasticity of the nervous system.AUTHOR SUMMARYMaps of gene expression patterns in the nervous system provide an important resource for neuron classification, for functional analysis and for developmental studies that ask how different neurons acquire their unique identities. By analyzing transgenic gfp reporter strains, we describe here the expression pattern of 244 putative chemosensory receptor-encoding genes, which constitute the largest gene family in C.elegans. We show that, as expected, chemoreceptor expression is enriched in chemosensory neurons but it is also expressed in a wide range of interneurons, motorneurons, as well as non-neuronal cells, suggesting that putative chemosensory receptors may not just sense environmental signals but also internal cues. We find that each chemoreceptor is expressed in a few neuron types, often just one, but each neuron type can express a large number of chemoreceptors. Interestingly, we uncovered that chemoreceptor expression is remarkably plastic, particularly in the context of the environmentally-induced dauer diapause stage. Taken together, this molecular atlas of chemosensory receptors provides an entry point for functional studies and offers a host of markers for studying neuronal patterning and plasticity.

scholarly journals Dolomitic filler in self-compacting concrete: a review

2020 ◽  
Vol 5 ◽  
pp. 75-83
Author(s):  
Ahmed Abdalqader ◽  
Mohammed Sonebi
Keyword(s):  
Key Factors ◽  
Self Compacting Concrete ◽  
Factors Affecting ◽  
Chemical Admixtures ◽  
Wide Range ◽  
Alkali Carbonate ◽  
Self Compatibility ◽  
Hardened Properties ◽  
By Products ◽  
Carbonate Reaction

The utilization of fine powders as fillers in self-compacting concrete (SCC) application is widespread, particularly in Europe. The incorporation of these fillers to attain the self-compatibility properties of SCC seems to be cheaper than the use of chemical admixtures. Among the wide range of potential fillers, dolomitic powders, particularly generated as by-products from quarry’s processing, are locally available and can be used to produce SCC. Few studies have shown that dolomitic powders can be incorporated in the SCC’s mix design, resulting in acceptable fresh and hardened properties of SCC. The particle size distribution and fineness of the dolomitic powder as well as the level of addition are the key factors affecting those properties. The influence of the chemical nature of the dolomitic powder on the properties of SCC, particularly the durability (e.g. alkali-carbonate reaction), is yet to be investigated. Furthermore, more efforts are still required to investigate the use of dolomitic by-products in the production of SCC.

scholarly journals The state of lipid exchange and thrombotic link of hemostasis in patients with chronic non-specific inflammatory diseases of genitals

2019 ◽  
Vol 7 (1) ◽  
pp. 34-45
Author(s):  
Irina Sokolovska ◽  
Nadezhda Maryukhnich ◽  
Valentyna Zarytska ◽  
Olena Kyrpychenko ◽  
Valentina Nechiporenko ◽  
...  
Keyword(s):  
Vascular Endothelium ◽  
Inflammatory Process ◽  
Blood Count ◽  
Inflammatory Diseases ◽  
Full Blood Count ◽  
Complex Treatment ◽  
Lipid Exchange ◽  
Lipid Fractions ◽  
Endothelium Damage ◽  
Blood Characteristics

It has been stated that changes of lipid exchange and increasing of lipid peroxidation are accompanied by the inflammatory process, which show the increase of proatherogenic blood characteristics. In patients with chronic non-specific inflammatory diseases of genitals disorders of phagocytosis mechanisms, increasing of autointoxication and reaction of hypersensitivity of immediate type are revealed after full blood count index. On the basis of analyses results one can conclude that the increase of atherogenic properties of blood plasma in patients with chronic non-specific inflammatory diseases of genitals took place in accordance with vascular endothelium damage. The article is the first to show the effectiveness of complex treatment of different atherosclerotic pathogenesis links by means of correction of proatherogenic lipid fractions and antioxidative complexes.

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