scholarly journals Aktivitas Antipiretik Ekstrak Etil Asetat Daun Seligi (Phyllanthus buxifolius Muell.Arg) Pada Mencit Jantan Galur Swiss

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Siwi Hastuti ◽  
Susi Endrawati
Keyword(s):  
Ethyl Acetate ◽  
Analgesic Activity ◽  
Ethyl Acetate Extract ◽  
White Male ◽  
Male Mice ◽  
Active Compounds ◽  
Antipyretic Effect ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Seligi (Phyllanthus buxifolius Muell. Arg) empirically proven to reduce pain and swelling in joints. The research of analgesic activity and antiinflammatory of seligi leaves was done, however antipyretic research not be found. The aim of this research was to examine the effect of ethyl acetate extract from seligi leaves as antipyretic on white male mice strain Swiss induced with DTP-HB-Hib vaccine. Ethyl acetate extract of seligi leaves doses of 100, 200 and 400 mg/kg showed antipyretic effect with values of 18.77 ± 1.99, 24.81 ± 4.34 and 35.39 ± 2.84 % respectively whereas paracetamol dose of 91 mg/kg have antipyretic effect with value of 50.38 ± 6.90 %. One way ANOVA test showed no significant difference for p (0.001) <p (0.05). Significance LSD Post hoc test for paracetamol 91 mg/kg with all seligi extract and extract seligi dose of 400 mg/kg with seligi extract 100 mg/kg have significant difference. While seligi extract 400 mg/kg with seligi extract 200 mg/kg and extract seligi 200 mg/kg with seligi extract 100 mg/kg no significant difference. Ethyl acetate extract of seligi leaves can be developed as source for active compounds with antipyretic activity.Key words: antipyretic, seligi, Phyllanthus buxifolius, temperature, mice. 

scholarly journals Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method

2020 ◽  
Vol 3 (2) ◽  
pp. 19-24
Author(s):  
Rezza Fikrih Utama ◽  
Rosidah ◽  
Yuandani
Keyword(s):  
Ethyl Acetate ◽  
White Male ◽  
Ethanol Extract ◽  
Immunomodulatory Activity ◽  
Male Mice ◽  
A Value ◽  
Significant Difference ◽  
Carbon Clearance ◽  
Phagocytosis Activity ◽  
Herb Extract

Poguntano Herb Plants (Picria fel-terrae Lour.) contains flavonoids compounds that have been potentially to developed into immunomodulators. The purpose of this study was to determine the immunomodulatory activity of Poguntano herb extract (Picria fel-terrae Lour.), with the method of carbon clearance. n-hexane extract puguntano herb(ENHHP), extract of ethyl acetate of Poguntano herb (EEAHP), and ethanol extract of puguntano herb(EEHP) Poguntano multistage results in the test of immunomodulatory activity with a method of carbon clearance. Results showed that the EEHP 200 mg/kg BB showed a strong immunostimulation effect, EEAHP 200 mg/kg BB showed strong immunostimulation effect, ENHP 200 mg/kg BB demonstrated strong immunostimulation effect. It is thought that extract N-hexane herb Poguntano (ENHHP), extracts of ethyl herb acetate Poguntano (EEAHP), and herb ethanol extract Poguntano (EEHP) Poguntano herb may increase phagocytosis activity. Extract of ethyl acetate Poguntano herb 200 mg/kg bw (EEAHP) has the best phagocytosis activity with a value of 2.376 which indicates there is a significant difference (P < 0.05) with another treatment group.

scholarly journals INVESTIGATION OF PHARMACOGNOSTICAL, PHYTOCHEMICAL, AND PHARMACOLOGICAL ACTIVITY OF AERIAL ROOTS OF FICUS BENGHALENSIS LINN

2018 ◽  
Vol 11 (10) ◽  
pp. 279
Author(s):  
Kamalika Mazumder ◽  
Himangshu S Maji ◽  
Nripendra N Bala
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Analgesic Activity ◽  
Ethyl Acetate Extract ◽  
Microscopic Investigation ◽  
Tail Flick ◽  
Ficus Benghalensis ◽  
Aerial Roots

Objective: Ficus benghalensis Linn. (Moraceae family) is commonly known as banyan tree in English, which is used traditionally in India. The literature survey showed that the aerial roots of this plant are yet to be explored. Our main interest is to evaluate its pharmacognostic and phytochemical character by the standard monograph and to explore its in vitro antioxidant and in vivo analgesic activity study with ethyl acetate extract.Methods: Pharmacognostic evaluation and phytochemical screening have been done using standard monograph. An in vitro antioxidant activity using ethyl acetate extract has been done using four different methods. In vivo analgesic activity of the ethyl acetate extract has been evaluated by acetic acid-induced writhing test in mice and tail flick method.Results: Aerial roots of F. benghalensis have been found the rich source of steroidal glycosides, cardiac glycosides, flavonoids, tri-terpenoids, and phenols. The presence of phellem, phellogen, xylem, and phloem has been found after microscopic investigation. All the pharmacognostic parameters proved its purity. Results showed the absence of heavy metals. The ethyl acetate extract has shown potent antioxidant activity at 100 μg/ml concentration and higher analgesic activity at the concentration of 400 mg/kg than 200 mg/kg.Conclusion: Pharmacognostic characteristics and phytochemical properties revealed in this study could be used for the pharmacopoeial standard. Ethyl acetate extract showed potent antioxidant and analgesic activity.

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus)

2004 ◽  
Vol 10 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Alifah Hayati ◽  
Binti Yunaida ◽  
I.B. Rai Pidada ◽  
Win Darmanto ◽  
Dwi Winarni
Keyword(s):  
Body Weight ◽  
Seminiferous Tubule ◽  
Intraperitoneal Administration ◽  
Primary Spermatocyte ◽  
Seminiferous Tubules ◽  
Male Mice ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Testicular Histology ◽  
One Way Anova

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.

scholarly journals Stomach Volume Evaluation on Fetal MRI and its use in Prenatal Identification of Esophageal Atresia

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Aditya Phadnis ◽  
Monica Forbes-Amrhein
Keyword(s):  
Esophageal Atresia ◽  
Fetal Mri ◽  
Third Trimester ◽  
Linear Measurements ◽  
Significant Difference ◽  
The Difference ◽  
Magnetic Resonance Imaging Mri ◽  
Stomach Volume ◽  
Post Hoc ◽  
One Way Anova

Swallowing of amniotic fluid alters the volume of the fetal stomach; therefore an abnormal fetal stomach size can be indicative of pathology. Previous studies have used linear measurements on ultrasound to approximate stomach sizes. Fetal magnetic resonance imaging (MRI) allows for volumetric measurement of the stomach. The objective of this study was to develop a library of normal fetal stomach volumes on MRI at each gestational age. We also sought to measure stomach volumes of fetuses with esophageal atresia and compare to normal volumes at similar gestation ages (GA).  A retrospective review of fetal MRIs from patients 20-38 weeks GA was conducted.  Exclusion criteria for the controls included any impairment that would impede swallowing or alter stomach sizes.  Patients with esophageal atresia were identified and postnatal records were reviewed to confirm the diagnosis. The stomach volume was measured on T2-weighted imaging using Phillips Intellispace software. The stomach volumes of the controls at each GA were compared using one-way ANOVA with Games-Howell Post-Hoc (p<0.05).  The stomach volume in esophageal atresia was compared to controls using a T-test (p<0.001).    185 control studies, 10 at each week of gestation, and 8 patients with esophageal atresia were included. For normal fetuses, there was a trend of increased stomach size and broadened standard deviation with increasing GA.  No significant difference was found between any two sequential weeks, however the difference in second and third trimester volumes was significant (p<0.001).   The patients with esophageal atresia had significantly smaller stomach volumes compared to control patients of the same GA range (p<0.001).   This pilot study established reference values for fetal stomach volume, which was found to increase with GA. The stomach volume is significantly smaller in patients with esophageal atresia.  Thus, esophageal atresia can be identified prenatally, allowing for delivery at an appropriate acuity NICU and early intervention.   

scholarly journals Potensi Genistein Terhadap Histopatologi Tubulus Seminiferus Testis Mencit (Mus musculus)

2012 ◽  
Vol 17 (2) ◽  
Author(s):  
Cicilia Novi Primiani ◽  
Umie Lestari
Keyword(s):  
Mus Musculus ◽  
Steroid Estrogen ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Pemanfaatan sarana kontrasepsi pria dengan memanfaatkan bahan alami berasal dari tanaman sudah banyak dilakukan. Tetapi pemanfaatan biji kedelai sebagai salah satu tanaman suku Leguminoceae belum pernah dilakukan. Biji kedelai mengandung senyawa genistein, sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17β-estradiol bersifat seperti hormon steroid estrogen, mampu menyebabkan kerusakan pada sistem reproduksi jantan. Penelitian bertujuan menguji pengaruh genistein terhadap histopatologi tubulus seminiferus testis mencit jantan (Mus musculus). Penelitian dilakukan menggunakan pendekatan eksperimental, yang rancangannya mengikuti rancangan acak lengkap. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikat adalah sel-sel germinal tubulus seminiferus testis. Data rerata jumlah sel-sel germinal dianalisis menggunakan Analisis Varians Satu Arah (One Way ANOVA) dengan tingkat signifikansi 95% (α 5%) dilakukan uji Post Hoc dengan Least Significant Difference (LSD) α 5%. Perubahan pada jaringan testis dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian genistein terhadap rerata jumlah sel-sel germinal dan menyebabkan penghambatan proliferasi sel-sel germinal dalam tubulus seminiferus testis.

scholarly journals Effect of Ethanol Extract and Ethyl Acetate Fraction of Betel Nut (Areca catechu L.) in Colonic Goblet Cells of Mice (Mus musculus) Given Orally Infective Egg of Trichuris muris

2020 ◽  
Vol 8 (B) ◽  
pp. 637-642
Author(s):  
Endy Juli Anto ◽  
Aznan Lelo ◽  
Syafruddin Ilyas ◽  
Marline Nainggolan
Keyword(s):  
Ethyl Acetate ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Goblet Cells ◽  
High Dose ◽  
Betel Nut ◽  
Male Mice ◽  
Trichuris Muris ◽  
Significant Difference

BACKGROUND: Trichuris trichiura is a soil-transmitted parasitic worm commonly found in humid, tropical to subtropical areas, as well as poor sanitation area. These worms are cosmopolitan, especially in tropical and humid countries like Indonesia. This worm infection is more common in developing countries and more in children than adults due to poorer child self-hygiene. Worm disease is one of the common society diseases with 60% of children in Indonesia which are estimated to be affected. One species of T. trichiura worm that is often used in research on experimental animals is Trichuris muris. The administration of T. muris infective eggs can significantly increase the number of goblet cells in intestinal crypts of colon and cecum. Goblet cell hyperplasia will occur after exposure to high-dose T. muris (acute/200 infective eggs) in resistant mice. Various studies on the development of natural and traditional materials have been developing in the past few years to see the effects of betel nut on the number of goblet cells in the large intestine. AIM: The aim of this research is to determine the effect of ethanol extract and the ethyl acetate fraction of betel nut on the change in the number of goblet cells in male mice given orally T. muris infective eggs. METHODS: This study was an experimental study with a post-test only control group design in male mice (Mus musculus) which were given 200 infective eggs of T. muris. The study sample consisted of 70 mice divided into seven groups. Doses of the ethanol extract of betel nut are 100 mg/kg b.w. and 150 mg/kg b.w., and doses of ethyl acetate fraction are 100 mg/kg b.w. and 150 mg/kg b.w. The independent variable was ethanol extract and ethyl acetate fraction of betel nut. The dependent variable is the number of goblet cells. Mean differences in groups were tested by Mann–Whitney. RESULTS: Statistical analysis showed a significant difference of p < 0.05 in the colonic crypts and cecum. In Group K (+), there was no increase in the number of goblet cells (54.2000 ± 30.54864) compared to Group K (−) (79.0750 ± 11.79221) in the colon. There was an increase in goblet cells in the 150 mg/kg b.w. of ethanol extract group (64.1000 ± 29.50381), the 150 mg/kg b.w. of ethyl acetate fraction group (56.2250 ± 22.11035), and the 1 mg/20 g of albendazole group (68.3750 ± 11.30956) in the colon. Likewise, there was an increase in the number of goblet cells in the cecum. It can be concluded that 150 mg/kg b.w. of the ethanol extract of betel nut can effectively increase the number of goblet cells in the colon and cecum. CONCLUSION: Ethanol extract of betel nut 150 mg / kg of weight can effectively increase the number of goblet cells in the colon and caecum.

scholarly journals ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA

2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman
Keyword(s):  
Leaf Extract ◽  
Periodontal Diseases ◽  
Tooth Surface ◽  
Control Group ◽  
Dilution Method ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.

scholarly journals TRANSPLANTATION OF ALLOGENIC ADIPOSE-DERIVED STEM CELL IMPROVE URETHRAL MUSCLE-COLLAGEN RATIO AFTER VAGINAL DISTENSION

2014 ◽  
Vol 21 (2) ◽  
Author(s):  
I Wayan Yudiana ◽  
Sunaryo Hardjowijoto ◽  
Sabilal Alif ◽  
Purwati Purwati ◽  
Willy Sandhika ◽  
...  
Keyword(s):  
Stem Cell ◽  
Striated Muscle ◽  
Study Groups ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
Behavior Frequency ◽  
Post Hoc ◽  
Adipose Derived Stem Cell ◽  
Vaginal Distension ◽  
One Way Anova

Objective:To investigate the effects of allogenic adipose-derived stem cell (ADSC) transplantation on urethral tissue regeneration after vaginal distension in a rat model.Material & Method:32 female wistar rats underwent vaginal distension (VD) for 4 h. Subsequently, PKH-labeled ADSCs (2 x 105 in 200 µl PBS, ADSC group, n = 18) or PBS (200 µl, placebo group, n = 18) were injected into periurethral tissue. Two and four weeks following transplantation (ADSC or PBS), voiding behavior (frequency) and muscle-collagen ratio of urethral tissue were measured to evaluate urethral sphincter regeneration. Data were analysed by paired-t test, one-way ANOVA and post hoc LSD.Results:Mean voiding frequency was significantly different in the ADSC group at two weeks (p<0.0001), but not significantly different at four weeks (p=0.448) when compared with the placebo group.Masson’s trichrome staining revealed that the muscle-collagen ratio of urethral tissue was not significantly different between study groups at two or four weeks (p=0.053 or p=0.166 respectively). Muscle-urethral ratio was more specific showing a significant difference at two weeks (p=0.043). There were significant differences about muscle-collagen or muscle-urethral ratio between control and placebo groups (p<0.05), whereas between control and ADSC groups no significant difference was observed (p>0.05).Conclusion:ADSC transplantation promotes urethral muscle-collagen ratio with development of striated muscle after vaginal distension, so that can improve voiding behavior in a rat model.Keywords:Adipose-derived stem cell, vaginal distension, rat-SUI model, muscle-collagen ratio, voiding stain on paper.

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