scholarly journals EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus)

2004 ◽  
Vol 10 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Alifah Hayati ◽  
Binti Yunaida ◽  
I.B. Rai Pidada ◽  
Win Darmanto ◽  
Dwi Winarni
Keyword(s):  
Body Weight ◽  
Seminiferous Tubule ◽  
Intraperitoneal Administration ◽  
Primary Spermatocyte ◽  
Seminiferous Tubules ◽  
Male Mice ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Testicular Histology ◽  
One Way Anova

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.

scholarly journals PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum) TERHADAP DIAMETER DAN TEBAL EPITEL TUBULUS SEMINIFERUS MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

2020 ◽  
Vol 8 (2) ◽  
pp. 132
Author(s):  
Aldis Ingrid Rusyawardani ◽  
Widjiati Widjiati ◽  
Suzanita Utama ◽  
Chairul Anwar ◽  
Epy Muhammad Luqman ◽  
...  
Keyword(s):  
Single Dose ◽  
Mus Musculus ◽  
Seminiferous Tubules ◽  
Average Weight ◽  
Male Mice ◽  
Treatment Groups ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Biophytum Petersianum ◽  
Epithelium Thickness

The aim of this research is to analyze the effect of kebar grass on the diameter and epithelium thickness of seminiferous tubules. Twenty male mice of BALB/C strain with an average weight of 25 gram were used in this research. Total five experimental groups (4 mice each group) were treated with combination of TCDD and kebar grass extract designed as follows: (C-) 0mg/g BW/day dan 0µg/kg BW, (C+) 0mg/g BW/day and 7µg/kg BW, (T1) 0,045mg/g BW/day and 7µg/kg BW, (T2) 0,080mg/g BW/day and 7µg/kg BW, (T3) 0,1350mg/g BW/day and 7µg/kg BW. The TCDD was injected peritoneally in single dose while kebar grass extract was given orally everyday for 53 days. The results showed a significant difference (p<0,05) on group which is treated with kebar grass extract 0,135mg/ g BW/ day is proven could maintain the diameter of seminiferous tubules in T3 group (170,5μmb ± 0,65μm) compared with C+ group (129,50μma ± 8,42μm). Significant difference of epithelial thickness were also found between the control and treatment groups (p<0,05). The C+ group (33,57μmb ± 1,61μm) was different from the T1 group (43,80μmc ± 1,46μm), T2 (48,49μmd ± 1,08μm), T3 (52,15μme ± 0,86μm) and C- (56,17μma ± 1,35μm). This findings might suggest TCDD has the potential to decrease the epithelium thickness and the diameter of seminiferous tubules, while kebar grass extract in various dose were proven to be able maintain the epithelium thickness of seminiferous tubules but could not maintain the diameter of seminiferous tubules.

scholarly journals EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS

2020 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
Dirga Januar Surya Utama ◽  
Suherni Susilowati ◽  
Tatik Hernawati ◽  
Erma Safitri ◽  
Sri Mulyati ◽  
...  
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
High Temperature ◽  
Analysis Of Variance ◽  
Adult Male ◽  
Mus Musculus ◽  
Primary Spermatocyte ◽  
Male Mice ◽  
Randomized Design ◽  
Significant Difference

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant  difference (p> 0.05).

scholarly journals Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

2021 ◽  
Author(s):  
Leyla Kilinc ◽  
Yesim Hulya Uz
Keyword(s):  
Body Weight ◽  
P38 Mapk ◽  
Seminiferous Tubule ◽  
Nuclear Factor Kappa B ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Nuclear Factor ◽  
Testicular Damage ◽  
Space Width ◽  
Tubule Diameter

Objective: Methotrexate (MTX), is a commonly used chemotherapeutic agent. This study aimed to investigate the possibility that curcumin (CMN) protects against MTX-induced testicular damage by affecting the phospho (p)-p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways. Methods: Male Wistar albino rats were subdivided into three groups. The control group was given an intragastric (ig) administration of dimethyl sulfoxide (DMSO) daily for 14 days, the MTX group was given a single intraperitoneal (ip) dose of MTX (20 mg/kg) on the 11th day, and the MTX+CMN group was given ig CMN (100 mg/kg/day, dissolved in DMSO) for 14 days and a single ip dose of MTX (20 mg/kg) on the 11th day. Results: The animal weights, the seminiferous tubule diameter, and germinal epithelium height significantly decreased in the MTX group compared to the control group. The testes weight and the ratio of the testes to body weight did not change, whereas the number of seminiferous tubules and the interstitial space width increased significantly in the MTX group. The number of phospho-p38 (p-p38) MAPK immunopositive cells and the immunoreactivity of NF-kB also increased in the MTX group compared to the control group. Conclusion: CMN prevented the MTX-induced decreases in the body weight, seminiferous tubule diameter, and the germinal epithelium height, while significantly reducing the number of histologically damaged seminiferous tubules and the interstitial space width changes due to MTX. CMN also reduced the number of p-p38 MAPK immunopositive cells and the NF-kB immunoreactivity.

scholarly journals Effects ofNigella sativa(Habbatus sauda) Oil and Nicotine Chronic Treatments on Sperm Parameters and Testis Histological Features of Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ng Cho Ping ◽  
Noor Hashida Hashim ◽  
Durriyyah Sharifah Hasan Adli
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Seminiferous Tubule ◽  
Corn Oil ◽  
Sperm Quality ◽  
Sperm Parameters ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Sprague Dawley ◽  
Histological Features

Twenty-fourSprague-Dawleymale rats (7–9 weeks old, 200–250 g) were divided into Nicotine (N) (0.5 mg/100 g body weight (BW), Nicotine Control (NC) (saline, 0.1 mL/100 g BW),Habbatus saudaoil (HS) (6.0 μL/100 g BW), andHabbatus saudaControl (HSC) (corn oil, 0.1 mL/100 g BW) groups and treated for 100 days. Sperm parameters and seminiferous tubules measurements were evaluated. The N showed a significantly lower sperm motility (1.03±0.05×106 sperm/mL) and percentage of normal (82.61±0.03%) and live (93.88±0.01%) sperm, higher value for the seminiferous tubule (253.36±1.83 μm) and lumen (100.15±2.38 μm) diameters and spermatogonia (19.85±0.39 μm) and spermatocytes (33.37±0.59 μm) layers, and thinner spermatid-sperm layer (22.14±0.71 μm) than the NC (P<0.05). The HS had significantly higher sperm motility (1.49±0.04×106 sperm/mL) and percentage of normal (90.61±0.01%) and live (96.98±0.01%) sperm, smaller lumen diameter (67.53±2.34 μm) and thinner spermatogonia (17.67±0.32 μm) and wider spermatid-sperm (36.95±0.79 μm) layers than the HSC (P<0.05). This research confirmed that nicotine reduced sperm motility and morphology of normal and live sperms and also affected the testis histology, whileHabbatus saudaoil increased sperm quality and gave better testis histological features.

Emesis in the Shrew Mouse (Suncus murinus) Induced by Peroral and Intraperitoneal Administration of Staphylococcal Enterotoxin A

1999 ◽  
Vol 62 (11) ◽  
pp. 1350-1353 ◽  
Author(s):  
DONG-LIANG HU ◽  
KATSUHIKO OMOE ◽  
HIDEAKI SHIMURA ◽  
KATSUHIKO ONO ◽  
SHUNJI SUGII ◽  
...  
Keyword(s):  
Animal Model ◽  
Body Weight ◽  
Intraperitoneal Administration ◽  
Staphylococcal Enterotoxin ◽  
Peroral Administration ◽  
Staphylococcal Enterotoxin A ◽  
Suncus Murinus ◽  
Male And Female ◽  
Significant Difference ◽  
Suitable Animal Model

Peroral and intraperitoneal administration of staphylococcal enterotoxin A (SEA) to Suncus murinus elicited an emetic response. The 50% emetic dose of SEA by peroral administration was found to be 32 μg per kg of body weight, whereas that by intraperitoneal administration was 3 μg per kg. Multiple emetic responses occurred 70 to 108 min after peroral administration of an emetic dose of SEA. Similar responses occurred 65 to 102 min after intraperitoneal injection of an emetic dose of SEA. No significant difference in vomiting was observed between male and female animals. Anti-SEA serum neutralized SEA-induced emesis in S. murinus. These findings indicate that S. murinus may serve as a suitable animal model to study the enterotoxigenicity of SEA.

Analysis of papaya plant sample for antispermatogenic properties

2020 ◽  
Vol 5 (2) ◽  
pp. 41-43
Author(s):  
Purushothaman M ◽  
Soujanya H ◽  
Jagadeeshwari S ◽  
Shiva Kumar K
Keyword(s):  
Body Weight ◽  
Guinea Pigs ◽  
Carica Papaya ◽  
World Health ◽  
Seminiferous Tubules ◽  
Plant Sample ◽  
Male Mice ◽  
Papaya Seeds ◽  
Health Organization ◽  
Papaya Seed

To advance new proof about the counter ripeness action of seeds concentrate of papaya (Carica papaya L.) in test creatures, particularly mice. Male mice (n=20) were assembled into four. The principal bunch got just purified water containing 1% CMC as the controller. Gathering 2, 3 and 4 sequentially got papaya seed concentrate of 2, 4 and 8mg/40 g body weight. All conduct regulated verbally utilizing abdominal sonde once every day for 35 days. Revision boundaries surveyed cell checks, the distance across and epithelial chunkiness of seminiferous tubules of the testis. Even though the aftereffects of exploration on the antifertility action of papaya seed separate demonstrated an optimistic pattern, yet in-depth confirmation impacts plant arrangements on investigation animals is obligatory suggested World Health Organization. This examination is proposed to advance novel proof counter fruitfulness movement of papaya seed removes in guinea pigs, particularly mice. There is no substantial diverse in the number of spermatogonia among treatment gatherings: spermatocyte and spermatid cells fundamentally diminished by the excerpt, particularly at the most elevated portion. Moreover, the breadth and epithelial thickness of seminiferous tubules of testis are significantly decreased by the concentrate at the portion of 8 mg/40g body weight. Papaya seeds separate as possible to be utilized as against ripeness operator.

scholarly journals Aktivitas Antipiretik Ekstrak Etil Asetat Daun Seligi (Phyllanthus buxifolius Muell.Arg) Pada Mencit Jantan Galur Swiss

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Siwi Hastuti ◽  
Susi Endrawati
Keyword(s):  
Ethyl Acetate ◽  
Analgesic Activity ◽  
Ethyl Acetate Extract ◽  
White Male ◽  
Male Mice ◽  
Active Compounds ◽  
Antipyretic Effect ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Seligi (Phyllanthus buxifolius Muell. Arg) empirically proven to reduce pain and swelling in joints. The research of analgesic activity and antiinflammatory of seligi leaves was done, however antipyretic research not be found. The aim of this research was to examine the effect of ethyl acetate extract from seligi leaves as antipyretic on white male mice strain Swiss induced with DTP-HB-Hib vaccine. Ethyl acetate extract of seligi leaves doses of 100, 200 and 400 mg/kg showed antipyretic effect with values of 18.77 ± 1.99, 24.81 ± 4.34 and 35.39 ± 2.84 % respectively whereas paracetamol dose of 91 mg/kg have antipyretic effect with value of 50.38 ± 6.90 %. One way ANOVA test showed no significant difference for p (0.001) <p (0.05). Significance LSD Post hoc test for paracetamol 91 mg/kg with all seligi extract and extract seligi dose of 400 mg/kg with seligi extract 100 mg/kg have significant difference. While seligi extract 400 mg/kg with seligi extract 200 mg/kg and extract seligi 200 mg/kg with seligi extract 100 mg/kg no significant difference. Ethyl acetate extract of seligi leaves can be developed as source for active compounds with antipyretic activity.Key words: antipyretic, seligi, Phyllanthus buxifolius, temperature, mice. 

scholarly journals PENGARUH PEMBERIAN JAMUR Fusarium graminearum TERHADAP HISTOPATOLOGI TUBULUS SEMINIFERUS MENCIT (Mus musculus)

2020 ◽  
Vol 8 (1) ◽  
pp. 54
Author(s):  
Fierda Kabayo ◽  
Abdul Samik ◽  
Ismudiono Ismudiono ◽  
Tjuk Imam Restiadi ◽  
Soeharsono Soeharsono ◽  
...  
Keyword(s):  
Body Weight ◽  
Veterinary Medicine ◽  
Sodium Chloride ◽  
Fusarium Graminearum ◽  
Mus Musculus ◽  
Histopathological Examination ◽  
Science And Technology ◽  
Seminiferous Tubules ◽  
Spermatogenic Cells ◽  
Male Mice

The aim of this research was to show the influence of seminiferous tubules histopathology of mice (Mus musculus) caused Fusarium graminearumexposure. This research was done in April-May 2017 in Microbilogy Laboratory Faculty of Science and Technology, Animal Laboratory, and Microbilogy Laboratory Faculty of Veterinary Medicine UniversitasAirlangga. This research used 20 male mice (Mus musculus) aged 6 weeks with 18-30 gram body weight. The mice devided into four groups: P0 given 0,25 ml Sodium chloride without Fusarium graminearum exposure by oral; P1 given 0,25 ml Fusarium graminearum exposure with dilution 102 by oral; P2 given 0,25 ml Fusarium graminearum exposure with dilution 103 by oral; and P3 given 0,25 ml Fusarium graminearum exposure with dilution 104 by oral. This treatment carried out for 21 days. Each milliliter dilution containing 228x106 spore for P1, 228x107 spore for P2, and 228x108 spore for P3. Then do the surgery and harvesting the testes then performed histopathological examination by scoring of seminiferous tubules. For data analyzing used non parametric difference Kruskall-Wallis and continued with Mann-Whitney. The result of this research was showed that decreased the spermatogenic cells in the process of spermatogenesis significant.

scholarly journals Skrining Fitokimia dan Uji Efektivitas Tepung Biji Kelor (Moringa oleifera L.) dalam Menurunkan Kadar Kolesterol Total Darah Mencit Jantan

2018 ◽  
Vol 7 (3) ◽  
pp. 122
Author(s):  
Nurliana Nurliana ◽  
Nurdin Rahman ◽  
Ratman Ratman
Keyword(s):  
Total Cholesterol ◽  
Test Group ◽  
Negative Control ◽  
Control Group ◽  
Blood Levels ◽  
Male Mice ◽  
Seed Flour ◽  
Significant Difference ◽  
Phytochemical Compounds ◽  
One Way Anova

The aim of the study was to determine phytochemical compounds levels in Moringa seeds and determine the effective dose of moringa seed flour in lowering total cholesterol blood levels of mice. Qualitative test of phytochemical compounds in moringa seed flour was done using specific reagents. The effect of moringa seed flour in lowering total cholesterol blood levels was performed in 18 male mice divided randomly into 6 groups, i.e P1 as the negative control group, P2 as the positive control group by giving simvastatin, P3 as the test group with a dose of 0.14 g/kg BW/day, P4 as the test group with a dose of 0.28 g/kg BW/day, P5 as the test group with a dose of 0.42 g/kg BW/day, and P6 as the test group with a dose of 0.56 g/kg BW/day. P1 to P6 were given propylthiouracilin conjunction with a suitable treatment of the group. Data were analyzed with One-way ANOVA and Duncan's post hoc tests at 95% significant level. Results showed that moringa seed flour contained positively of flavonoids and saponins. Results analysis of changes on the total cholesterol levels between groups with One-way ANOVA test showed a significant difference (p = 0.002), where Fcount value=7.476 > Ftable value=3.11. The group with the provision of 0.42 g/kg BB/day of moringa seed flour performed the most effective influence in lowering total cholesterol blood levels of male mice which amounted to 34.33 mg/dL or 18.66%.

scholarly journals The influence of aqueous extract of roselle (Hibiscus sabdariffa) calyx on blood cholesterol level and body weight in rats

2011 ◽  
Vol 9 (2) ◽  
pp. 43-49
Author(s):  
MEISA MARSALINA ◽  
SAMIGUN SAMIGUN ◽  
ENDANG SRI HARDJANTI
Keyword(s):  
Body Weight ◽  
Cholesterol Level ◽  
Aqueous Extract ◽  
Statistical Test ◽  
Blood Cholesterol ◽  
Average Weight ◽  
Hibiscus Sabdariffa ◽  
Blood Cholesterol Level ◽  
Significant Difference ◽  
One Way Anova

Marsalina M, Samigun, Hardjanti ES. 2011. The influence of aqueous extract of roselle (Hibiscus sabdariffa) calyx on blood cholesterol level and body weight in rats. Biofarmasi 9: 43-49. This study aimed to investigate the influence of aqueous extract of roselle calyx on blood cholesterol level and body weight in rats. This experimental research was arranged as a pre and post-test controlled group design. Thirty Sprague-Dawley rats, with 3 months of age and 250 g in average weight, were used as an animal model. Those rats were grouped equally into five groups, i.e. negative control (aquadest), positive control (0.26 mg lovastatin/200 g body weight of rats/2 mL), the first dose of aqueous extract of roselle calyx (65 mg/200 g/2 mL), the second dose of aqueous extract of roselle calyx (130 mg/200 g/2 mL), and the third dose of aqueous extract of roselle calyx (195 mg/200 g/2 mL). The measurement of rat blood cholesterol level was conducted before and after treatment, while the rat body weight was measured once a week. The result of this study was analyzed by using one-way ANOVA statistical test. The one-way ANOVA statistical test showed no significant difference on reducing blood cholesterol level with p=0.327 (p>0.05) and body weight in rat with p=0.154, p=0.214, and p=0.938 (p>0.05). This study concluded that the aqueous extract of roselle calyx has no influence on lowering blood cholesterol level and body weight in rats.

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