Study of analgesic and anti-inflammatory activity of dry extract from sakhalin willow shoots

One of the important treatment landscape widely used for the various disease’s management is phytotherapy, or treatment with herbal medicines. Phytotherapy is used as an independent type of treatment in most cases, and in combination with other drugs as additional treatment. The pain and inflammation control continue as long as there is humanity itself. With old experience of traditional medicine know that Salicaceae have a pronounced anti-inflammatory and analgesic effects. The pharmacological study of the dry extract from Sakhalin willow shoots – the study of analgesic and anti-inflammatory activities in different pharmacological models – was the aim of this work. The analgesic and anti-inflammatory activity of the standardized dry extract from Sakhalin willow shoots (DESWS) was experimentally researched. The study of pharmacological activities of DESWS was carried out at the Educational and Scientific Institute of Applied Pharmacy of the National University of Pharmacy (Kharkov). The effect of DESWS on the course of the inflammatory process was studied in a model of carrageenan edema. Analgesic activity was studied in a model of thermal irritation in white male rats. The study of anti-inflammatory activity in rats on a model of carrageenan edema showed that an effective dose of the studied extract was 30 mg/kg. The greatest reduction in paw edema in rats on the model of carrageenan edema in the first hour of the experiment was observed in the group of animals treated with DESWS at a dose of 30 mg/kg. The anti-inflammatory activity of DESWS was 53.74%, but it was slightly inferior than in the reference drug Diclofenac. It was found that DESWS also shows pronounced analgesic properties at a dose of 30 mg/kg during the first and second hours of the experiment in the model of the limb’s thermal irritation «Hot Plate» in rats. After 120 minutes of experiment the studied activity didn’t change significantly and remained at the level 62.6%. The analgesic effect of DESWS was inferior to antinociceptive activity of the reference drug Metamizole sodium and superior to the studied activity of the reference drug Aspirin. Thus, the dry extract from the Sakhalin willow shoots can affect both pain and inflammation, and it is a potential remedy for further pharmacological research.

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Investigation of anti-inflammatory and antipyretic activity of polymorphic modification of n-(trifluoromethylphenyl)-4-hydroxy-2,2-dioxo-1h-2λ6,1-benzothiazine-3-carboxamide derivative in rats

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2021-25(1)-05 ◽

2021 ◽

Vol 25 (1) ◽

pp. 27-31

Author(s):

P. S. Bondarenko ◽

N. І. Voloshchuk

Keyword(s):

Inflammatory Reaction ◽

White Male ◽

Statistical Processing ◽

Biologically Active ◽

Inflammatory Activity ◽

Polymorphic Modification ◽

Reference Drug ◽

Antipyretic Effect ◽

Antipyretic Activity ◽

Anti Inflammatory

Annotation. Polymorphism among biologically active substances is an extremely important factor that modifies the therapeutic properties of pharmaceutical substances and dosage forms, significantly affects the parameters of their biological activity and pharmacokinetics. Chemical synthesis among the derivatives of 4-R-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acids gave the leader compound N-(4-trifluoromethylphenyl)-4-hydroxy-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxamide, the crystalline polymorph of which in the form of plates has a pronounced analgesic activity. The aim of the study was to investigate the anti-inflammatory activity of compound B by its effect on various components of the inflammatory reaction, as well as the antipyretic effect of this compound in rats. The anti-alterative activity was determined on the model of planar skin wounds, the antiproliferative effect - on the model of cotton granuloma, the anti-exudative effect - on the model of toxic pulmonary edema in rats. The antipyretic effect was studied in a model of milk fever in rats. The study was performed on 91 white male Wistar rats. Statistical processing of the obtained results was performed using the program "STATISTICA 6.1" by methods of variation statistics using parametric and nonparametric methods. The results showed that the polymorphic compound B has anti-inflammatory activity on all components of the inflammatory reaction - alteration, exudation and proliferation. In terms of efficacy in the model of experimental inflammation, this substance is superior to meloxicam, as its ED50 when administered orally is 5.2 mg/kg, while the reference drug - 9.1 mg/kg. Compound B exhibits a pronounced antipyretic effect in a rat fever model. Therefore, the polymorphic modification of the carboxamide derivative in the form of plates is promising for further research in order to create on its basis a drug with analgesic, anti-inflammatory and antipyretic properties.

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Antiinflammatory activity of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates on carrageenan model of inflammation

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.4.19.09 ◽

2019 ◽

pp. 82-87

Author(s):

B. V. Pristupa ◽

I. O. Shyshkin ◽

Ya. V. Rozhkovsky ◽

V. O. Gelmboldt

Keyword(s):

Antiinflammatory Activity ◽

Biologically Active ◽

Inflammatory Activity ◽

Male Rats ◽

Control Group ◽

Paw Edema ◽

Commercial Preparation ◽

Reference Drug ◽

Anti Inflammatory ◽

Inflammatory Effect

In the last decade, high cariesprophylactic efficacy of ammonium hexafluorosilicates, including salts with substituted biologically active ammonium cations, has been demonstrated. Among the promising anti-caries agents are recently synthesized 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates, for whose cations anti-inflammatory activity is expected. The aim of the work is to assess the presence of anti-inflammatory activity in the series of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates in the experiment. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates (I–III, respectively) were synthesized according to the previously proposed technique, carrageenan is a commercial preparation, reference drug is a indomethacin. Experiments on the anti-inflammatory activity of hexafluorosilicates were performed on 77 white Wistar male rats weighing 174–190 g using the carrageenan model of inflammation. The inflammatory reaction was reproduced by subplantary administration of 0.1 ml of a 0.2% solution of carrageenan, the studied compounds were administered orally in doses 1/10, 1/20, 1/50 from LD50 for the compound III. It was established that after administration of carrageenan, animals of the control group showed marked paw edema, which gradually increased and was maximal after 24 hours of observation. According to the data obtained, the indices for compounds I-III practically do not differ from those of the control group. This indicates that, despite the results of the PASS forecast and the presence of acetic acid residue, an anti-inflammatory pharmacofor, in compounds I-III, the compounds under study do not have an anti-inflammatory effect in the carrageenan model. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates do not show the expected anti-inflammatory activity in the carrageenan model of inflammation.

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In vivo anti-inflammatory activity of anti-atherosclerotic herbs using white male rats (Rattus norvegicus)

10.1063/1.5139348 ◽

2019 ◽

Author(s):

Dewi Tristantini ◽

Riska Amalia

Keyword(s):

Rattus Norvegicus ◽

White Male ◽

Inflammatory Activity ◽

Male Rats ◽

Anti Inflammatory

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Anti-inflammatory Activity of Ethanol Extract of Lotus (Nelumbo nucifera G.) Seed Against White Male Rats Using Paw Edema Method

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i4.4918 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1-4

Author(s):

Khairani Fitri ◽

Tetty Noverita Khairani ◽

Kristin Tiurma Sianturi ◽

Leny Leny ◽

Ihsanul Hafiz

Keyword(s):

White Male ◽

Ethanol Extract ◽

Nelumbo Nucifera ◽

Inflammatory Activity ◽

Male Rats ◽

Control Group ◽

Paw Edema ◽

White Rats ◽

Activity Test ◽

Anti Inflammatory

The purpose of this study was to determine the anti-inflammatory activity test of ethanol extract of lotus seeds in rats induced by carrageenan. The research method used was experimental. The object used in this study was male white rats. Observations were made for 6 hours and then the data were analyzed using the one way analysis of variance (ANOVA) test. The anti-inflammatory activity test was carried out on the test animals which were divided into 5 groups, 3 each with the suspension test group with a dose of 200, 300, 400 mg/kg bw, the negative control group was the CMC Na 1% suspension and the positive control was diclofenac Na 2, 25 mg/kg bw. The results showed that the ethanol extract of the seeds of lotus (Nelumbo nucifera G) was proven to have an anti-inflammatory effect on carrageenan-induced male white rats at doses of 200, 300 and 400 mg/kg BW with the percentage value of inflammation inhibition at the 6th hour was 65.79, 74.65 and 80.77%, with the most effective dose in reducing anti-inflammatory drugs being 400 mg/kg bw. The ethanol extract of the seeds of lotus (Nelumbo nucifera) has anti-inflammatory activity. Keywords: anti-inflammatory activity, Nelumbo nucifera, paw edema

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UJI AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN PISANG AMBON (Musa paradisiaca L.) TERHADAP TIKUS PUTIH (Rattus norvegicus L.) YANG DIINDUKSI KARAGENAN

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2015.v1.i2.6244 ◽

2015 ◽

Vol 1 (2) ◽

pp. 126-132 ◽

Cited By ~ 1

Author(s):

Sukmawati Sukmawati ◽

Yuliet Yuliet ◽

Ririen Hardani

Keyword(s):

White Male ◽

Ethanolic Extract ◽

Negative Control ◽

Inflammatory Activity ◽

Male Rats ◽

Musa Paradisiaca ◽

Activity Test ◽

Significant Difference ◽

Anti Inflammatory ◽

Banana Leaf

Anti-inflammatory activity test of ethanolic extract of banana leaf (Musa Paradisiaca L.) on carrageenan-induced paw edema in white male rats (Rattus novergicus L.) has been conducted. It was aimed to investigate and to determine the anti-inflammatory activity and its effective dose. The extract was prepared by maceration method using ethanol 96%. Anti-inflammatory activity test was performed in five different groups. Each group consisted of 5 rats. The 1st group (negative control) was given 0.5% CMC-Na suspension; the 2nd group (positive control) was given diclofenac sodium 9 mg/KgBW; the 3rd, 4th, and 5th groups were successively given the banana leaf extract as much as 500, 750 and 1000 mg/KgBW. Each rat was then induced by 1% carrageenan and tested using subplantar method. The inflamed paw diameter was measured using a calliper while the inflamed paw volume using pletysmometer. The measurements were done for 6 hours long with intervals of 60 minutes. The data was statistically analyzed using ANOVA (analysis of variance). The results showed that the negative control had a significant difference with the other treatment groups which did not show any anti-inflammatory effect. In conclusion, ethanolic extract of banana leaf has effective anti-inflammatory activity at a dose of 750 mg/KgBW

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Anti-Inflammatory Activity of The Extract of Guava Leaves (Psidium guajava L) in The Rat (Rattus norvegicus L)

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev2iss1pp169-172 ◽

2011 ◽

Vol 2 (1) ◽

pp. 169 ◽

Cited By ~ 3

Author(s):

Linda Weni ◽

Harliansyah Harliansyah ◽

Widayanti Widayanti

Keyword(s):

White Male ◽

Psidium Guajava ◽

Positive Control ◽

Inflammatory Activity ◽

Male Rats ◽

Degenerative Diseases ◽

Sprague Dawley ◽

Anti Inflammatory ◽

Guava Leaves ◽

Different Doses

Using of natural sources that have anti-inflammatory activity for the prevention and treatment of degenerative diseases began to be further explored. An investigation on the anti-inflammatory activity of the aqueous extract of guava leaves (Psidium guajava L.) from Sawangan, Depok on white male rats of Sprague-Dawley strain had been carried out on the carrageenan-induced paw edema method. To examine the effect of guava extract on subcutan at different doses of 125, 250 and 500 mg/kg of body weight (BW). Indometacine at dose of 10 mg/kg BW was used as a positive control. Observations were made during five hours with an interval of one hour. These results demonstrate that the percentage of inflammation or edema (% E) optimal at the 4th hour and then decreased at the 5th hour, while the percentage of optimal inhibition occurred at the 5th hour. Guava extract at 125, 250 and 500 mg/kg BW reduced inhibitory percentage activities by 40.81, 55.45 and 43.61% (p< 0.05) respectively. In conclusion, this study suggests that guava extract has anti-inflammatory properties by decreasing edema level.Keywords: Anti-inflammatory, guava leaves, edema.

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Scientific Evaluation of anti-inflammatory potential of Macrotyloma uniflorum seeds extracts

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4393 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 1883-1888

Author(s):

Anandarajagopal K ◽

Abdullah Khan ◽

Sugalia S ◽

Bama Menon ◽

Tan Ching Siang ◽

...

Keyword(s):

Antioxidant Properties ◽

Water Extract ◽

Ethanol Extract ◽

Inflammatory Activity ◽

Leguminous Plant ◽

Paw Edema ◽

Reference Drug ◽

Macrotyloma Uniflorum ◽

Phenolic Constituents ◽

Anti Inflammatory

Phytochemicals possessing the antioxidant properties naturally present in food attract a greater interest to healthcare researchers due to their desirable health effects on human health as they can be explored for protection against oxidative deterioration. Macrtotyloma uniflorum is a leguminous plant belonging to the family Fabaceae and commonly known as Horse gram. Aqueous and ethanol extracts of seeds of Macrotyloma uniflorum were evaluated for their anti-inflammatory effects using the scientific protocol on experimental rats. Extraction was carried out using the cold maceration method, and the anti-inflammatory activity was evaluated using a digital plethysmometer in the experimental rats injected with carrageenan to produce paw edema. Preliminary phytochemical studies confirmed the presence of various bioactive compounds such as alkaloids, glycosides, carbohydrates, proteins and amino acids, terpenoids, tannins, and phenolic compounds in both extracts while flavonoids were found only in ethanol extract. Both extracts of M. uniflorum seeds (200 mg/ml) significantly (p<0.01) reduced the paw edema volume induced by carrageenan. The ethanol extract of M. uniflorum seeds exhibited more potent anti-inflammatory activity than water extract, that might be due to the presence of flavonoids in ethanol extract. The activity of the extracts was compared with diclofenac sodium (10mg/kg b.wt.) as a reference drug. From the results, it may be suggested that the antioxidative potential of phenolic constituents and flavonoids is the primary factors for the anti-inflammatory activity of M. uniflorum seeds extracts.

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AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v7i2.56 ◽

2019 ◽

Author(s):

Haka As'ada ◽

Yardi Saibi ◽

Hendri Aldrat

Keyword(s):

Dose Range ◽

White Male ◽

Ethanol Extract ◽

Negative Control ◽

Inflammatory Activity ◽

Male Rat ◽

Control Group ◽

Angelica Keiskei ◽

Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

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Study to Investigate the anti-inflammatory effect of Codelac® Broncho with Thymus Serpyllum (elixir) in comparison with reference drug Fenspiride (syrup) using accute Carrageenan-induced Paw Inflammation Model

Research Results in Pharmacology ◽

10.3897/rrpharmacology.5.33233 ◽

2019 ◽

Vol 5 (1) ◽

pp. 45-52

Author(s):

Pavel D. Kolesnichenko ◽

Anna A. Peresypkina ◽

Artem A. Poromov ◽

Elena N. Kareva ◽

Alexey N. Demidenko

Keyword(s):

Inflammatory Activity ◽

C Reactive Protein ◽

Reference Drug ◽

Reactive Protein ◽

Model Control ◽

Anti Inflammatory ◽

Thymus Serpyllum ◽

Carrageenan Inflammation ◽

Subplantar Injection ◽

Inflammatory Effect

Introduction: Evaluation of anti-inflammatory action of Codelac® Broncho with Thymus Serpyllum (elixir) in comparison with Fenspiride was carried out on the model of acute carrageenan inflammation of the paws in rats. Materials and methods: Edema was caused by subplantar injection of 0.1 ml of 1% λ- carrageenan gel into the hind paw. The severity of edema was assessed by using 37140 plethysmometer (UGO BASILE, Italy). The measurements were performed before edema induction and 1, 2, 4, 12, 24, 48, 72, 96 and 120 hours afterwards. Anti-inflammatory activity of the drugs was also evaluated based on the analysis of rats’ blood, C-reactive protein concentration and histological examination results. Results and discussion: A decrease in the paw volume increment was revealed in the group with the studied drug in comparison with the group with the carrageenan edema model (control) 4, 12, 24 hours after injection of carrageenan (p<0.05). As a result of plethysmometry, a more pronounced anti-inflammatory effect of the studied drug than that of Fenspiride was revealed. There was a significant decrease in the levels of leukocytes (p<0.05), lymphocytes (p<0.05), monocytes (p<0.05) and neutrophils (p<0.05) in the group with the studied drug compared to those the the control 48 hours after the initiation of edema, while in the group with Fenspiride, there was only a decrease in the levels of leukocytes (p<0.05) and lymphocytes (p<0.05). There were no differences in the concentration of C-reactive protein between the groups. Conclusion: The obtained data indicate a more pronounced anti-inflammatory activity of Codelac® Broncho with Thymus Serpyllum in comparison with Fenspiride, on the model of acute carrageenan inflammation of the paw in rats.

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STANDARDIZATION AND STABILITY EVALUATION OF DRY EXTRACTS OF MYRACRODRUON URUNDEUVA ALLEMÃO OBTAINED BY SPRAY DRIER

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15065 ◽

2017 ◽

Vol 9 (2) ◽

pp. 154 ◽

Cited By ~ 1

Author(s):

Renata Da Silva Leite ◽

Valmir Gomes De Souza ◽

Agna HÉlia De Oliveira ◽

JosÉ VenÂncio Chaves JÚnior ◽

Islaine De Souza Salvador ◽

...

Keyword(s):

Silicon Dioxide ◽

Feed Rate ◽

Stability Study ◽

Inflammatory Activity ◽

Inlet Temperature ◽

Dry Extract ◽

Anti Inflammatory ◽

The Stability ◽

Drying Conditions ◽

Quercetin Concentration

Objective: This study aimed to obtain standardised dry extracts of Miracrodruon urundeuva Allemão using spray-dryer and evaluate the stability of the extracts.Methods: It evaluated the drying parameters: Proportion of colloidal silicon dioxide (CSD) (10, 15 and 20%), inlet temperature (160, 170 and 180 °C) and feed rate (4, 6 and 8 ml/min). The study of the accelerated stability of dry extract occurred in temperature of 40 °C (±2 °C) and relative humidity of 75% (±5%) for 6 mo. The anti-inflammatory activity of the dry extract was evaluated in Swiss mice by the paw edema method.Results: Variations in drying conditions did not represent significant variations in yields of the process. The drying temperature and feed rate significantly influenced the concentration of quercetin (p≤0.05). The increase in inlet temperature and feed flow promoted the increase of quercetin concentration in the extracts. The stability study showed that the concentration of quercetin in dry extract was stable over a period of 6 mo. The dry extract showed anti-inflammatory activity in mice orally.Conclusion: A condition of 10% of colloidal silicon dioxide with an 180 °C inlet temperature and a feed rate of 8 ml/min was considered the most adequate for obtaining the extracts and the drying process resulted in stable dry extracts and the quercetin was a suitable biomarker for monitoring the process.

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