Phytochemical analysis of the main groups of secondary metabolites in the Dracocephalum moldavica L. herb

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.5.21.09 ◽

2021 ◽

pp. 85-93

Author(s):

М. І. Шанайда

Keyword(s):

Phenolic Compounds ◽

Secondary Metabolites ◽

Essential Oil ◽

High Performance ◽

Raw Material ◽

Phytochemical Analysis ◽

Chemical Parameters ◽

Physical And Chemical Parameters ◽

Physical And Chemical ◽

Layer Chromatography

The genus Dragonhead (Dracocephalum L.) includes 72 species of essential oil-bearing plants belonging to the Lamiaceae family. Many of them have long been used in a folk medicine of different countries. Moldavian dragonhead (D. moldavica L.) has been identified as the most promising species for the complex phytochemical research since it was successfully introduced in the climatic conditions of the Ukrainian forest-steppe zone and due to the presence of its various botanical forms and varieties. The aim of this study was the phytochemical analysis of the main groups of secondary metabolites in the herb of violet-flowered form of Moldavian dragonhead (D. moldavica L.) under its cultivation in Ternopil region (Ukraine). The herb of the plant was harvested in July of 2018‒2019 years during the flowering period. The essential oil was obtained from the plant raw material by the hydrodistillation. Such physical and chemical parameters of the essential oil as a relative density, refractive index, optical rotation, acid number and solubility in ethanol were studied. The method of thin layer chromatography (TLC) was used to determine the specific sequence of bands in the chromatograms of essential oil. The method of high-performance thin-layer chromatography (HPTLC) was used for the identification of phenolic compounds in the herb. The component composition of polyphenols was determined by the high performance liquid chromatography (HPLC). The specific physical and chemical parameters as well as the «chromatographic profile» of essential oil have been established. The main compounds of the polyphenolic nature were revealed by the chromatographic methods. In particular, rosmarinic and caffeic hydroxycinnamic acids were identified in the methanol extract of herb by the HPTLC method. The HPLC analysis of phenolic compounds in the 70% ethanol extract of raw material established that rosmarinic acid was its main polyphenol. It was found the decreasing of polyphenol contents in the D. moldavica herb in such direction: rosmarinic acid (3.01%) > apigenin-7-О-glucoside (0.67%) > apigenin (0.29%) > caffeic acid (0.28%) > luteolin-7-О-glucoside (0.13%)> hyperoside (0.12%) > ferulic acid (0.09%) > chlorogenic acid (0.08%). The obtained data could be used for planning pharmacological studies of the phytosubstances developed from the Moldavian dragonhead herb.

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Using high performance thin layer chromatography for the detection of pharmacologically active secondary metabolites in Empetrum nigrum L.

Drug development & registration ◽

10.33380/2305-2066-2021-10-4(1)-129-137 ◽

2021 ◽

Vol 10 (4) ◽

pp. 129-137

Author(s):

A. O. Ponkratova ◽

A. K. Whaley ◽

V. G. Luzhanin ◽

E. V. Zhokhova

Keyword(s):

Secondary Metabolites ◽

Medicinal Plant ◽

Thin Layer ◽

High Performance ◽

Phytochemical Analysis ◽

Empetrum Nigrum ◽

Aerial Parts ◽

Pharmacologically Active ◽

Layer Chromatography ◽

Derivatives Of

Introduction. The article presents the results of the detection of pharmacologically active secondary metabolites in black crowberry Empetrum nigrum L. using the method of high performance thin layer chromatography (HPTLC).Aim. To show the efficiency of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites in promising medicinal plant species.Materials and methods. HPTLC analysis was carried out on a CAMAG device (Switzerland), using MERCK HPTLC silica gel 60 F154, 20 × 10 cm plates. For the evaporation of the samples, a Heidolph vacuum rotary evaporator (Germany) was used. The aerial parts (shoots) of Empetrum nigrum were harvested next to St. Petersburg State Chemical and Pharmaceutical University (SPCPU) nursery garden of medicinal plants (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in August 2019.Results and discussion. In the course of the research, four fractions from the aerial parts of Empetrum nigrum were obtained: hexane, dichloromethane, butanol, and water. Then, these fractions were investigated by HPTLC in two solvent systems – n-butanol : acetic acid : water (BAW) (4 : 1 : 2) and hexane : dichloromethane : methanol (HDM) (1 : 2 : 0.5). After scanning densitometric analysis of the plates eluted in the HDM system, it was revealed, that the hexane and dichloromethane fractions have a similar composition and contain the greatest amount of compounds, compared to the butanol and water fractions, and in the BAW system, it was found, that the butanol fraction contains the greatest variety of metabolites. As a result of UV spectroscopy, it was found, that the main groups of compounds contained in the hexane and dichloromethane fractions are derivatives of chalcones, dihydrochalcones, bibenzyls and 9,10-dihydrophenanthrenes. While in the butanol fraction, the main groups of secondary metabolites were derivatives of flavonoids and tanninsConclusion. The data obtained allow us to note the efficiency, speed and simplicity of HPTLC for conducting preliminary phytochemical analysis to determine the main groups of metabolites of promising medicinal plant species.

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Evaluation of Physical and Chemical Parameters According to Energetic Willow (Salix viminalis L.) Cultivation

Energies ◽

10.3390/en14102968 ◽

2021 ◽

Vol 14 (10) ◽

pp. 2968

Author(s):

Kamil Roman ◽

Michał Roman ◽

Dominika Szadkowska ◽

Jan Szadkowski ◽

Emilia Grzegorzewska

Keyword(s):

Industrial Development ◽

Raw Materials ◽

Renewable Energy Sources ◽

Consumer Products ◽

Raw Material ◽

Salix Viminalis ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Parameters ◽

Physical And Chemical Parameters ◽

Physical And Chemical

The aim of this study was the investigation of the effect of growth conditions of energy willow (Salix viminalis L.) on its physical and chemical parameters towards lignocellulosic biofuels. This work is linked to the global trend of replacing fossil fuels (coal, oil, natural gas) with energy and renewable fuels. This energy transition is dictated by the reduction of the human-induced greenhouse effect (to the greatest extent by industrial development). Changing from traditional to renewable energy sources results in industry becoming less dependent on fuels whose sources are beginning to run out, and in energy processing being broken down into smaller sectors with greater flexibility to change and less susceptibility to failure. The use of lignocellulosic raw materials such as wood, straw, food industry waste, wood, and post-consumer products such as old furniture for energy purposes allows the use of substances which bind the greenhouse gas carbon dioxide in their cellular structure during growth. In order to optimise the costs of producing such energy and minimise its impact on the environment, these plants should be located as close as possible to the source of raw materials. One of the most important characteristics for the profitability of energy production from woody biomass is a high biomass yield. For this purpose, the raw material used for this study was energy willow (Salix viminalis L.) seedlings, which are often used for energy crops. Due to the moisture-loving nature of the substrate, the effect of the addition of the active substance prednisonum as a catalyst for water adsorption from the substrate was investigated. In order to determine the substances formed during the thermal decomposition of energy willow (Salix viminalis L.) wood, a pyrolysis process was carried out at 450 °C using pyrolysis gas chromatography mass spectrometry (PY/GC-MS).

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PHYTOCHEMICAL ANALYSIS OF SECONDARY METABOLITES OF SATUREJA HORTENSIS L.

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15923 ◽

2017 ◽

Vol 9 (2) ◽

pp. 315

Author(s):

Mariya Shanaida ◽

Iryna Ivanusa ◽

Ivanna Kernychna

Keyword(s):

Secondary Metabolites ◽

Essential Oil ◽

Essential Oils ◽

High Performance ◽

Aerial Part ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Satureja Hortensis ◽

Main Component ◽

Essential Oil Yield

Objective: The present study was designated to analyse the essential oils and tannins as important secondary metabolites of the aerial part of Satureja hortensis.Methods: The chemical composition of S. hortensis herb was investigated using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). The essential oil was obtained by hydrodistillation method.Results: The essential oil yield in the aerial part of S. hortensis was 1.61%. GC-MS analysis of the essential oils identified the presence of 29 components. Carvacrol (76.16%), as the main component of essential oils, belongs to the group of aromatic compounds. Eight tannin components identified by HPLC and epigallocatechin (130.91х10-2%) are prevalent among them.Conclusion: S. hortensis was found to possess considerable amount of phytoconstituents such as essential oils and tannins. The results of this research will help to study pharmacological properties of the investigated plant and to prevent possible adulteration with other plants.

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The assessment of the amount of water pollution and its suitability for drinking of the Tyśmienica River Basin, Poland

Environmental Monitoring and Assessment ◽

10.1007/s10661-021-09034-w ◽

2021 ◽

Vol 193 (5) ◽

Author(s):

Antoni Grzywna ◽

Joanna Sender

Keyword(s):

River Basin ◽

High Performance ◽

Ph Value ◽

Ecological Status ◽

Polluted Water ◽

Chemical Parameters ◽

Measuring Point ◽

Physical And Chemical Parameters ◽

Good Ecological Status ◽

Physical And Chemical

AbstractThe quality and potability of waters of the Tyśmienica River Basin were determined in 2017 at eight measuring stations. The paper presents analyses of the physical and chemical parameters of surface waters of the basin. With regard to suspended solids, pH value, electric conductivity, sulphates, ammonia, chlorides and phosphate, the water was classified as having a very good ecological status. In the case of Kjeldahl nitrogen, the waters were classified as having a good ecological status. In the case of the remaining parameters, i.e. BOD, DO, TOC and COD, the status varied among stations. The values of all the physical and chemical parameters complied with the values for undisturbed conditions. Only ammonia and COD showed significant differences among stations. The WPI values for each measuring point ranged from 0.90 to 1.56, what corresponds to the descriptive indicators of moderately polluted water. The high concentrations of COD and TOC indicate that high-performance treatment processes (category A3) must be used to ensure that the water is suitable for drinking.

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EVALUATION CRITERIA OF WINE MATERIALS INTENDED FOR THE PRODUCTION OF RED SPARKLING WINES

Сборник избранных статей по материалам научных конференций ГНИИ "Нацразвитие" (Санкт-Петербург, Февраль 2021) ◽

10.37539/feb295.2021.27.48.005 ◽

2021 ◽

Author(s):

Елена Васильевна Дубинина ◽

Варвара Алексеевна Захарова ◽

Ирина Андреевна Ротару ◽

Александра Александровна Чистова

Keyword(s):

Phenolic Compounds ◽

Red Wine ◽

Evaluation Criteria ◽

Chemical Parameters ◽

Color Characteristics ◽

Chemical Indicators ◽

Physical And Chemical Parameters ◽

Physical And Chemical ◽

Chromatic Color ◽

Comprehensive Study

На основании комплексного исследования физико-химических показателей красных виноматериалов для игристых вин, в том числе состава фенольных соединений и хроматических характеристик цвета, научно обоснована целесообразность использования дополнительных физико-химических показателей при оценке виноматериалов, предназначенных для производства красных игристых вин. Based on a comprehensive study of the physical and chemical parameters of red wine materials for sparkling wines, including the composition of phenolic compounds and chromatic color characteristics, the feasibility of using additional physical and chemical indicators in the evaluation of wine materials intended for the production of red sparkling wines is scientifically justified.

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INFLUENCE OF FOOD ADDITIVE “MAGNETOFOOD” ON PHYSICAL AND CHEMICAL PARAMETERS, FUNCTIONAL PROPERTIES OF MODEL MINCED MEAT SYSTEMS

Food Science and Technology ◽

10.15673/fst.v13i2.1389 ◽

2019 ◽

Vol 13 (2) ◽

Author(s):

I. Tsykhanovska ◽

L. Skurikhina ◽

V. Evlash ◽

L. Pavlotska

Keyword(s):

Binding Capacity ◽

Acid Number ◽

Food Additive ◽

Raw Material ◽

Chemical Parameters ◽

Technological Parameters ◽

Water Binding ◽

Physical And Chemical Parameters ◽

Minced Meat ◽

Physical And Chemical

. The influence of a food additive“Magneftofud” (nanopowder of double oxide of two- and trivalent Ferum) on physical and chemical parameters, functional and technological properties of model beef minced meat have been studied. Also, their organoleptic parameters and the output of ready steaks made from them were investigated. Food additive “Magnetofood” was added to the beef minced meat in the amount of 0.05–0.15% to the mass of meat raw material. The water-binding ability was determined by the method of pressing; degree of oxidation by means of peroxide and acid numbers; output of the finished product and organoleptic indicators – by standard and commonly accepted methods; digestion of proteins of steaks – by the method of Pokrovsky A.A. and Yertanova E.D. It has been determined that due to the nanosize of Ferum oxide , a significant specific volume, developed active surface, affinity for proteins, thermal stability, the additive “Magnetofood” has antioxidant, sorption, complexing, structure-forming, stabilizing, water-binding, water and fat-retaining properties. Thus, the investigated additive contributes to the formation of new functional and technological indicators of beef meat systems and the improvement of consumer characteristics of the finished products obtained from them. The input of "Magnetofood" food additive in beef minced meat improves the organoleptic and functional-technological parameters compared to control: the moisture-binding capacity increases by 12.0–12.8%; output of finished products - by 1,5–4,5%. The growth of the total effect of digestion of proteins of prepared steaks (in 1,17-1,65 times) have been noted; reduction of the acid number (by 3,0–4,0%) and peroxide number (by 0,09–0,12 mmolol/kg). The results of the studies allowed to determine the rational amount of food supplement “Magnetofood”, it is 0.15% to the mass of meat raw materials.

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The peculiarities of the technology of bread production from frozen semi-finished products of a high degree of readiness with the use of functional components

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/845/1/012113 ◽

2021 ◽

Vol 845 (1) ◽

pp. 012113

Author(s):

E S Taranova ◽

N V Labutina ◽

E A Kuznetsova ◽

E N Efremova ◽

E A Zenina

Keyword(s):

Wheat Flour ◽

Raw Materials ◽

Raw Material ◽

Wheat Bread ◽

Optimal Dosage ◽

Chemical Parameters ◽

Baked Goods ◽

Physical And Chemical Parameters ◽

Physical And Chemical ◽

Functional Components

Abstract Ensuring the health of the population is a task of the national importance. One of the most significant factors that determine a person’s health and performance is nutrition. In a highly competitive environment, manufacturers face one of the main tasks - the creation of new competitive products with high-quality indicators and preventive properties. Improving the quality of baked goods is one of the problems of bakery production. The article deals with the theoretical possibility and practical feasibility of using spelt flour in the production of baked goods with improved consumer benefits. Spelt flour is a valuable raw material for the production of bakery and flour confectionery products of increased nutritional and biological value. The use of whole grain spelt flour in the recipe of wheat bread allows creating products for dietary and curative and preventive purposes. In the course of the work, experimental baking of wheat bread with different dosages of wheat and spelt flour was carried out. Samples of wheat bread with a different combination of spelt and wheat flour of the highest grade in the ratios of 5:95; 10:90; 15:85% were studied. The research was conducted in the laboratory of the Department of Technology of Storage and Processing of Agricultural Raw Materials and Public Catering, Volgograd State Agrarian University. The results of the influence of spelt flour application on the organoleptic and physical and chemical parameters of wheat bread are presented. As a result of the conducted research, the possibility of producing wheat bread using spelt flour was established, and the optimal dosage of spelt and wheat flour which allows one to obtain bread with high organoleptic and physical and chemical parameters was identified.

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Characteristics, Phytochemical Analysis and Biological Activities of Extracts from Tunisian ChetouiOlea europaeaVariety

Journal of Chemistry ◽

10.1155/2015/418731 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 9

Author(s):

Ines Khlif ◽

Karim Jellali ◽

Thomas Michel ◽

Maria Halabalaki ◽

Alexios Leandros Skaltsounis ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

High Performance ◽

Biological Activities ◽

Antioxidant Property ◽

Antimicrobial Activities ◽

Phytochemical Analysis ◽

Polar Extracts ◽

Layer Chromatography

This study selected 10 extracts from Tunisian chetouiO. europaeavariety for their total phenolics, flavonoids, and phytochemical analyses as well as for their antioxidant and antimicrobial activities determination. Thein vitroantioxidant property was investigated using DPPH, ferric reducing antioxidant capacity (FRAP), oxygen reducing antioxidant capacity (ORAC), andβ-carotene-linoleic acid bleaching assays while antimicrobial activity was evaluated using macrodilutions method. For all organs of chetouiO. europaeavariety, the investigated activities were found to be higher in the polar extracts (ethyl acetate, methanol, and methanol/water). These activities were correlated with the presence of phenolic compounds. Phytochemical analyses revealed that the crude extracts contain triterpenoids, quinones, and flavonoids. High performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) confirmed the presence of phenolic compounds in the studied extracts.

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