scholarly journals Perbaikan performa reproduksi ikan komet (Carassius auratus auratus Linnaeus 1758) melalui pemberian tepung otak sapi sebagai GnRH alami

2018 ◽  
Vol 17 (3) ◽  
pp. 289
Author(s):  
Ujang Subhan ◽  
Yuli Andriani ◽  
Kiki Haetami ◽  
Rosidah Rosidah ◽  
Abi Muhamad Abdillah

This study aimed to determine the effect of brain flour cow’s on feed in the performance of eggs and fecundity in fish comet. The research was conducted at the Faculty of Fisheries and Marine Sciences, University of Padjadjaran at November 2014 until January 2015. Freeze-drying of bovine brains was conducted at the Inter Research Center Uni-versity(PAU), Bandung Institute of Technology. The research was using Completely Randomized Design con-sisting of four treatments and three replications. The treatment were 20 mg kg-1, 35 mg kg-1, 50 mg kg-1 and control. Parameters observed were the distribution of the eggs, egg diameter and fecundity using gravimetric methods. Data were analyzed using analysis of variance. If there are differences among the treatments then continued with Duncan's multiple range test at 5% level. Addition of bovine brain meal in feed with dose of 50 mg kg-1 was give a dominant influence on the size distribution of eggs by 60% from 1.01 to 1.5 mm, egg diameter 0.90 mm, and fecundity of 67 grains g-1. AbstrakPenelitian ini bertujuan untuk menentukan dosis tepung otak sapi pada pakan yang berpengaruh terhadap performa telur dan fekunditas ikan komet. Penelitian dilaksanakan di panti benih Fakultas Perikanan dan Ilmu Kelautan Univer-sitas Padjadjaran pada bulan November 2014 sampai dengan Januari 2015. Pengeringan beku otak sapi dilakukan di Pusat Penelitian AntarUniversitas Institut Teknologi Bandung. Penelitian ini menggunakan metode eksperimental de-ngan Rancangan Acak Lengkap yang terdiri atas 4 perlakuan dan 3 ulangan. Perlakuan yang diberikan yaitu 20 mg kg-1 induk, 35 mg kg-1 induk, 50 mg kg-1 induk dan perlakuan kontrol. Parameter yang diamati yaitu sebaran telur, diameter telur, dan fekunditas relatif menggunakan metode gravimetrik. Data dianalisis menggunakan analisis keragaman dengan uji F untuk mengetahui pengaruh dari setiap perlakuan. Apabila terdapat perbedaan antar perlakuan maka dilanjutkan dengan uji jarak berganda Duncan pada taraf 5%. Penambahan tepung otak sapi pada pakan sebanyak 50 mg kg-1 induk memberikan pengaruh dominan sebaran telur pada ukuran 1,01-1,5 mm sebesar 60%, diameter telur 0,90 mm, dan fe-kunditas relatif sebesar 67 butir g-1.

AQUASAINS ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 769
Author(s):  
Febri Setyawati ◽  
Kismiyati Kismiyati ◽  
Sri Subekti

Abstrak Goldfish (Carassius auratus) is one of the fishes that can be infected by parasites. Commonly, this fish is often attacked by Argulus japonicus ectoparasites. Control of infestation in A. japonicus can be done by utilizing one of the natural ingredients, Moringa oleifera leaf extract, which contains of alkaloids, tannins, saponins and flavonoids. This study aims to determine the effect of M. oleifera leaf extract in reducing the infestation of A. japonicus and the optimal concentration of M. oleifera leaf extract in reducing the infestation of A. japonicus in goldfish (C. auratus). This study used a completely randomized design (CRD) with treatments namely control, 600 ppm, 700 ppm, 800 ppm and 900 ppm with four times of replication. The main parameter is the percentage decrease in infestation of A. japonicus. The results of the research data were analyzed by using ANOVA and followed with the Ducan Multiple Range Test. The results showed that Moringa leaf extract could reduce the infestation of A. japonicus. The optimal concentration of Moringa leaf extract in reducing the infestation of A. japonicus was obtained from a concentration of 700 ppm.


2018 ◽  
Vol 22 (1) ◽  
pp. 72
Author(s):  
Herika Novrelly Jayatri ◽  
Christanti Sumardiyono ◽  
Arif Wibowo

Banana is one of the important fruit crop in Village of Sidomulyo, Bantul, Yogyakarta. One of important diseases which become the constraint in development of banana is Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc). This fungus has high race diversity and  virulence, so that it required early detection for prevention and control of disease. This experiment was aimed to figure out race and virulence of Foc isolates from Village of Sidomulyo, Bantul, Yogyakarta. The 13 tested isolates were isolates of PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, and A80. Race was molecularly detected using two types of primers, i.e. General Foc primer FocEf3 and specific primer for race 4 (Foc-1/Foc-2). Virulence test was performed on banana seedlings of Ambon Kuning cultivar using Completely Randomized Design (CRD) with 14 treatments and 4 repetitons. The observed parameters were external and internal symptoms, calculation of disease severity index and disease intensity. Data were analyzed using variance and further test of Duncan Multiple Range Test (DMRT) at 5 % level. The results showed that all isolates were Foc and 9 of 13 isolates were grouped into race 4, i.e. A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, and PR43. The highest and lowest virulences were consecutively expressed by PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, and A80, with severity index on leaves and rhizomes ranging 1.61-2.91 and 2.25-7, respectively. IntisariPisang merupakan tanaman buah unggulan di Desa Sidomulyo Kecamatan Bambanglipuro, Kabupaten Bantul. Salah satu penyakit penting yang menjadi kendala dalam pengembangan pisang adalah layu fusarium yang disebabkan oleh jamur Fusarium oxysporum f. sp. cubense (Foc). Jamur ini memiliki keragaman ras dan virulensi yang tinggi, sehingga deteksi dini diperlukan untuk pencegahan dan pengendalian penyakit. Penelitian ini bertujuan untuk mengetahui ras dan virulensi isolat Foc asal Desa Sidomulyo, Kecamatan Bambanglipuro, Kabupaten Bantul. Isolat yang diuji sebanyak 13 isolat, yakni isolat PR11, PKJ20, RU20, PR30, AH40, PKJ40, A41, RB42, PR43, RU51, A60, RP60, dan A80. Pengujian ras secara molekuler dengan menggunakan dua jenis primer yakni primer Foc in general FocEf3 dan primer spesifik ras 4 Foc-1/Foc-2. Uji virulensi pada bibit kultivar ambon kuning dengan menggunakan Racangan Acak Lengkap (RAL) yang terdiri dari 14 perlakuan dan 4 ulangan. Parameter yang diamati berupa pengamatan gejala luar dan gejala dalam, penghitungan indeks keparahan penyakit dan intensitas penyakit. Analisis data menggunakan sidik ragam dan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf 5 %. Hasil pengujian menunjukkan bahwa semua isolat merupakan isolat Foc dan dari 13 isolat yang digunakan terdapat 9 isolat yang merupakan ras 4 yakni isolat A80, RP60, PR11, A41, AH40, PKJ40, PR30, RB42, dan PR43. Isolat yang memiliki virulensi tertinggi sampai terendah berturut-turut adalah PR30, RB42, RU51, RP60, PR43, PKJ40, PR11, A41, AH40, RU20, PKJ20, A60, dan A80, dengan indeks keparahan pada daun berkisar 1,61−2,91 dan indeks keparahan pada bonggol 2,25−7.


2017 ◽  
Vol 11 (2) ◽  
pp. 39-49
Author(s):  
Tyas Nyonita Punjungsari

The purpose of this research is to understanding the effect of organic matter on BPS activity in reducing sulfate. Research carried out in batch culture using erlenmeyer, and using a completely randomized design (CRD). The treatment given is organic matter amounted to 308 mg / L, 617 mg / L and 1.234 mg / L and control. Each treatment was repeated 3 times. The parameters observed in this study is SO4- concentration. Using Duncan New Multiple Range Test (DNMRT) at 5% level for data analysis. The results showed that the molecular concentration of 617 mg / L was able to decrease the sulfate concentration at the fastest, then consecutively the concentration of molasses 1.234 mg / L, and control. Based on the Anova test the significant value is less than 0.05. Thus it can be stated that there is an effect of addition molasses to decrease sulfate concentration by sulfate reducing bacteria consortium.


2017 ◽  
Vol 11 (2) ◽  
pp. 39-49
Author(s):  
Tyas Nyonita Punjungsari

The purpose of this research is to understanding the effect of organic matter on BPS activity in reducing sulfate. Research carried out in batch culture using erlenmeyer, and using a completely randomized design (CRD). The treatment given is organic matter amounted to 308 mg / L, 617 mg / L and 1.234 mg / L and control. Each treatment was repeated 3 times. The parameters observed in this study is SO4- concentration. Using Duncan New Multiple Range Test (DNMRT) at 5% level for data analysis. The results showed that the molecular concentration of 617 mg / L was able to decrease the sulfate concentration at the fastest, then consecutively the concentration of molasses 1.234 mg / L, and control. Based on the Anova test the significant value is less than 0.05. Thus it can be stated that there is an effect of addition molasses to decrease sulfate concentration by sulfate reducing bacteria consortium.


2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Dadik Pantaya., dkk

The present study was conducted to clarify the effect of addition of enzymes from rumen liquor on performance broiler of pelleting feed with basal ration of wheat pollard with addition of enzymes and processing broiler diets. Ruminal enzymes were extracted from Australian Commercial Cross (ACC) cattles. Application of enzyme on wheat pollard. Enzyme was reacted with wheatpollard and mixed with another feedstuff before steam pelleting process. The combination nine experimental diets were used as follows three levels enzyme supplementation (0, 620 and 1.240 U/kg) and three levels steam temperature at (60, 80 and 100 oC) Feed particles size, hardness, PDI (Pellet Durability Index), moisture content, protein, GE and hemicellulosa were evaluated parameter devided into nine treatmens were used in this research. Data from Completely Randomized Design factorial 3 x 3 were analyzed with Anova, if differences were continued by Duncan’s new multiple range test. Enzyme supplementation decreased feed hardness and PDI. There were interaction beetwen enzyme and temperature for feed moisture content. Hemicellulosa was significantly reduced by steam temperature at 80 and 100 oC and enzyme supplementation 1.240 U/kgKey Words: enzyme liquor rumen, wheat pollard, steam pelleting, feed Chemical composition


2021 ◽  
Vol 19 (1) ◽  
pp. 155-163
Author(s):  
S Sapkota ◽  
D Kc ◽  
H Giri ◽  
M Saud ◽  
M Basnet ◽  
...  

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)


2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Wd Wd Sitti Aisyah Nurul Fahlani ◽  
Tamrin Tamrin ◽  
Kobajashi Togo Isamu

ABSTRACT                     This study aimed to analyze the effect of lindur fruit flour substitution on organoleptic characteristics, nutritional value, and antioxidant activity of lindur fruit flour brownies. This research was conducted using a completely randomized design (CRD) with various percentages of formulation of substitution of lindur fruit flour:wheat flour, namely M1 = (100%:0%), M2 = (90%:10%), M3 = (80%:20 %), M4 = (70%:30%), and M5 = (60%:40%). The research data were analyzed using analysis of variance, with further testing using Duncan's Multiple Range Test (DMRT) at a 95% confidence level. The results show that the HCN content of lindur fruit flour reached 21.81 mg/kg. The M3 treatment was the most preferred treatment by the panelists with hedonic rating scores of color, texture, aroma, and taste reached 4.14 (like), 4.13 (like), 3.79 (like), and 4.30 (like), respectively. The nutritional values of selected M3 brownies show that it contained 30.57% water, 1.20% ash, 3.70% fat, 5.63% protein, and 58.90% carbohydrate. Analysis of antioxidant activity in selected M3 brownies was 465.58 ppm which is categorized as very weak. It can be concluded that the substitution of lindur fruit flour in making brownies had a very significant effect on color, texture, and taste. However, the effect was not significant on the aroma and the analysis of the nutritional value of the selected treatment. The brownie product substituted with lindur fruit flour met the national standards for water, ash, and fat contents.Keyword: Brownies, Lindur Fruit ABSTRAK    Penelitian ini bertujuan untuk mempelajari pengaruh substitusi tepung buah lindur terhadap karakteristik organoleptik, nilai gizi, dan aktivitas antioksidan brownies tepung buah lindur. Penelitian ini dilakukan dengan menggunakan Rancangan Acak  Lengkap  (RAL) dengan berbagai presentase formulasi, substitusi tepung buah lindur: tepung terigu yaitu M1 =  (100% : 0%), M2 = (90% : 10%), M3 = 80% : 20%), M4 = (70% : 30%), dan M5 = (60% : 40%). Data hasil penelitian dianalisis menggunakan sidik ragam (Analysis of Varian), dengan uji lanjut menggunakan Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95%. Hasil penelitian menunjukan analisis kadar HCN tepung buah lindur yaitu 21,81 mg/kg. Perlakuan M3 merupakan perlakuan yang paling disukai oleh panelis dengan skor penilain hedonik warna 4,14 (suka), tekstur  4,13 (suka), aroma 3,79 (suka) dan rasa 4,30 (suka). Analisis Nilai gizi produk browies tepilih M3 meliputi kadar air (30,57%), kadar abu (1,20), Kadar lemak (3,70%), kadar protein (5,63%) dan kadar karbohidrat (58,90%). Analisis aktivitas antioksidan pada brownies terplih M3 yaitu 465,58 ppm dengan kategori sangat lemah. Dapat disimpulkan bahwa substiusi tepung buah lindur pada pembuatan brownies berpengaruh sangat nyata terhadap warna, tekstur, dan rasa. Tetapi berpengaruh tidak nyata terhadap aroma. dan analisis nilai gizi perlakuan terpilih. Berdasarkan standar mutu SNI brownies, bahwa produk brownies substitusi tepung buah lindur sudah memenuhi standar mutu SNI untuk kadar air dan kadar abu serta kadar lemak.Kata kunci:   Brownies, tepung, Buah Lindur


2016 ◽  
Vol 69 (2) ◽  
pp. 8007-8014 ◽  
Author(s):  
José G. Serpa ◽  
Tulia I. Pérez ◽  
Elvis J. Hernández

The effect of pasteurization and starter cultures on physicochemical, microbiological and sensorial characteristics of costeño cheese was determined. A completely randomized design was conducted, three treatments (T) and three replicates: Treatment 1 (T1): cheese manufactured with pasteurized milk without starter cultures, Treatment 2 (T2): cheese manufactured with pasteurized milk with Lactococcus lactis and Lactococcus cremoris (1:1) and Treatment 3 (T3): cheese manufactured with pasteurized milk with Lactococcus lactis, Lactococcus cremoris and Streptococcus thermophillus(0.5:0.5:1). Treatments were compared to a control sample that was prepared with raw milk without starter cultures. Concentration of 1.5% (v/v) of culture was used in relation to the amount of used milk in each treatment. Moisture content was higher in all treatments compared to the control and protein and fat content were significantly lower. Acidity was significantly higher in samples from T2 y T3 compared to T1 and control, due to the metabolism of starter cultures. Total coliforms, yeast and mold counts showed a significant reduction due to pasteurization process in all treatments. Regarding sensorial analysis, hedonic test showed a greater preference in cheese manufactured with T2 (P<0.05). There were no significant preferences between T1, T3 and control. Additionally, yield was significantly higher with T1 (22%) and T3 (23%) compared to control.


2019 ◽  
Vol 20 (1) ◽  
pp. 1-5
Author(s):  
Wuye Ria Andayanie

Black grass jelly plants can be used as conservation plants. However, uret pests (Leucopholis rorida F.) are often complained that eat the base of the stems, flowers and roots at night. This study aims to determine: 1) the effect of M. anisopliae fungi on uret pests, (2) the level of susceptibility of uret pests, and (3) M. anisopliae fungal effectiveness on uret pests. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. Data from the observations were analyzed using analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) 5% test carried out when diversity analysis showed a real effect. Uret mortality monitoring will be carried out after the application of fungi. by observing each uret and calculating the amount of uret mortality and average plant growth. The highest percentage of second instar larvae from uret was found in treatment P4 (manure and fungi were incubated for 72 hours at the next temperature of 28 oC applied to the field with straw mulch) which was 82, 98% at 12 days after application of fungi. The average growth of plants consisting of: root length, root volume, stem diameter and number of shoots was also found in treatment P4 (manure and fungi were incubated for 72 hours at the next 28 oC, applied to the field with straw mulch), respectively amounting to 12.45; 5.70; 1.88; 12,15. Dead larvae emit fluids such as ethanol and their bodies are weak, then the body dries and hardens and stiffens, like mummies and fungi forming green sporulation. Keywords—: black grass jelly plants; Leucopholis rorida F;Metharhizium anisopliae; mortality;effectiveness


2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Aam Gunawan

Maggot black soldier fly (Hermetia illucens) is a protein source of feed which is highly favored by poultry, especially ducks and chickens. However, it is feared that the provision of live maggot in ducks will affect the organoleptic quality of the egg, especially its taste and aroma. Therefore this study aims to determine the organoleptic quality of duck eggs fed Hermetia illucens maggot feeds in a living state. The study used 120 alabio ducks which were placed in a postal cage. The design used was a completely randomized design, each treatment using six replications. Each replication is taken egg sample to be tested panelists. The panelists used were 67 panelists who were somewhat trained. The data obtained were analyzed of variance and Duncan's multiple range test. The treatments that were tried consisted of P1: low protein rations without live maggot, P2: low protein rations with live maggot administration 40 g/bird/day, P3: high protein rations without live maggot administration, and P4: high protein rations with live maggot administration 40g/bird/day. The results showed that the treatment affected the texture, flavor, and aroma of boiled eggs. Ducks fed with high protein ration coupled with the provision of live maggot 40 g/bird/day produce softer textures, tastes quite good, and aroma more fishy. Keywords: Maggot, duck eggs, organoleptics


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