scholarly journals The Effect of Saffron (Crocus Sativus) on Oxymetholone-induced Hepatic and Renal Injury in Rats

2021 ◽  
Vol 15 (4) ◽  
pp. 233-240
Author(s):  
Reza Kheirandish ◽  
◽  
Mehdi Saberi ◽  
Shahrzad Azizi ◽  
Reza Khakdan ◽  
...  
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Muscle Growth ◽  
Antioxidant Properties ◽  
Degenerative Changes ◽  
Crocus Sativus ◽  
Protective Effects ◽  
Negative Effects ◽  
Male Wistar Rats ◽  
Histopathological Results

Background: Oxymetholone, an anabolic-androgenic steroid, has been used to treat some diseases. The abuse of this compound, especially for muscle growth, has severe oxidative side effects on the liver and kidneys. Oxidative stress and free radicals are responsible for the development of various diseases. Phytochemicals are sources of polyphenols, flavonoids, saponins, etc. and may act as scavengers of free radicals. Saffron (Crocus sativus) has considerable antioxidant properties, which may be useful in reversing or preventing these negative effects. Methods: To evaluate the effects of saffron on the liver and kidneys degenerative changes induced by oxymetholone, 24 male Wistar rats were used. The rats were randomly divided into four groups of six rats each as: a) sham (normal saline, 40 mg/kg/day), b) saffron control (40 mg/kg/day), c) oxymetholone (50 mg/rat), and d) treatment group [saffron (40 mg/kg/day)+oxymetholone (50 mg/rat)]. The course of examination was 30 days. Results: After one month, the sham and saffron control groups had normal histological findings. The treatment group with saffron showed a significant reduction in the hepatic and renal degenerative changes induced by oxymetholone compared to those observed in group. Conclusion: Based on the histopathological results, the use of saffron produced protective effects against the degenerative changes in rats’ liver and kidneys induced by oxymetholone.

The Protective Effects of Traditional Chinese Medicine Prescription, Han-Dan-Gan-Le, on CCl4-induced Liver Fibrosis in Rats

1998 ◽  
Vol 26 (03n04) ◽  
pp. 325-332 ◽  
Author(s):  
Cheng-Xiu Li ◽  
Ling Li ◽  
Jung Lou ◽  
Wen-Xiou Yang ◽  
Ting-Wen Lei ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Liver Fibrosis ◽  
Human Liver ◽  
Antioxidant Properties ◽  
Protective Effects ◽  
Morphological Alterations ◽  
Collagen Accumulation ◽  
Medicine Prescription ◽  
Male Wistar Rats ◽  
Chinese Medicine Prescription

Han-Dan-Gan-Le, a Chinese medicine preparation composed of Salvia miltorrhiza, Radix paeoniae, Astragalus membranaceus, Stephania tetrandra, and dried leaves of Ginkgo biloba, has been used successfully to treat human liver fibrosis and cirrhosis for years. This study was designed to examine the mechanisms of the protection. Male Wistar rats were given CCI4 (1.2 ml/kg, 2 times/week), 20% fat diet, and 30% alcohol in drinking water (every other day) for 6 weeks. Han-Dan-Gan-Le (0.5 and 1.0 g/kg, p.o., daily for 6 weeks) was administered to rats simultaneously to examine the protective effects against CCl4-induced liver fibrosis. The experimentally-induced liver fibrosis and other morphological alterations were significantly ameliorated by Han-Dan-Gan-Le. Han-Dan-Gan-Le treatments decreased CCl4-induced hepatic collagen accumulation by more than 50%, and significantly increased urinary excretion of hydroxyproline. The CCl4-induced lipid peroxidation in liver and serum was ameliorated as a result of Han-Dan-Gan-Le treatment, possibly by restoring the activity of superoxide dismutase activity in liver and erythrocytes, In conclusion, Han-Dan-Gan-Le is effective in protecting against liver fibrosis. The mechanisms of the protection appear to be due to its antioxidant properties and the modulation of hepatic collagen metabolism.

scholarly journals Protective Effect of Sundarban Honey against Acetaminophen-Induced Acute Hepatonephrotoxicity in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Rizwana Afroz ◽  
E. M. Tanvir ◽  
Md. Fuad Hossain ◽  
Siew Hua Gan ◽  
Mashud Parvez ◽  
...  
Keyword(s):  
Honey Bees ◽  
Renal Damage ◽  
Serum Alkaline Phosphatase ◽  
Antioxidant Properties ◽  
Organ Damage ◽  
Modern Medicine ◽  
Complex Compounds ◽  
Protective Effects ◽  
Renal Morphology ◽  
Male Wistar Rats

Honey, a supersaturated natural product of honey bees, contains complex compounds with antioxidant properties and therefore has a wide a range of applications in both traditional and modern medicine. In the present study, the protective effects of Sundarban honey from Bangladesh against acetaminophen- (APAP-) induced hepatotoxicity and nephrotoxicity in experimental rats were investigated. Adult male Wistar rats were pretreated with honey (5 g/kg) for 4 weeks, followed by the induction of hepatotoxicity and nephrotoxicity via the oral administration of a single dose of APAP (2 g/kg). Organ damage was confirmed by measuring the elevation of serum alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), total protein (TP), total bilirubin (TB), urea, creatinine, and malondialdehyde (MDA). Histopathological alterations observed in the livers and the kidneys further confirmed oxidative damage to these tissues. Animals pretreated with Sundarban honey showed significantly markedly reduced levels of all of the investigated parameters. In addition, Sundarban honey ameliorated the altered hepatic and renal morphology in APAP-treated rats. Overall, our findings indicate that Sundarban honey protects against APAP-induced acute hepatic and renal damage, which could be attributed to the honey’s antioxidant properties.

scholarly journals The Protective Effects of Turmeric on Liver Enzymes of Metronidazole-Treated Adult Male Wistar Rats

2015 ◽  
Vol 1 (6) ◽  
pp. 255
Author(s):  
Akudike C. J. ◽  
Chukwu V. O. ◽  
Ezejindu D. N. ◽  
Ihim A. C.
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Liver Enzymes ◽  
Antimicrobial Effect ◽  
The Body ◽  
Protective Effects ◽  
Negative Effects ◽  
Serum Samples ◽  
Male Wistar Rats ◽  
Anti Oxidant

Over the years, research works on the different liver enzymes has proven to be very helpful to man. This study evaluates the protective effects of turmeric on the liver enzymes of metronidazole-treated adult male wistar rats. With previous researches, metronidazole has been found to exert some negative effects on some organs of the body like the testis, kidney etc but the turmeric on its own part, has been found to have no side effects with a host of beneficial functions such as its anti-oxidant and antimicrobial effect amongst a host of other functions. In this study, twenty wistar rats with weight range of between 165-180g were assigned into four groups A, B, C and D of 5 each. The experimental groups A, B and C were orally administered 200mg of metronidazole, 400mg of metronidazole and 400mg of metronidazole as well as 400mg of turmeric respectively for duration of twenty-eight days while the group D served as control and were orally administered water and feed only. Twenty four hours after the last administration, the animals were anaesthetized under chloroform inhalation and dissected for organ collection. Blood for serum preparation was collected into sterile plain tubes and stored in the refrigerator for analysis. Serum samples from the bloods were analyzed for liver enzymes activities using randox kit method. The study revealed that turmeric helped reduced the effects of metronidazole on the serum activities of ALT, AST and ALP on the liver. This present study suggests that metronidazole could have negative effects on the liver and so advises that turmeric be added when on metronidazole administration.

scholarly journals Perbedaan efek pemberian tunggal vitamin E dengan pemberian kombinasi vitamin e dan zink terhadap kualitas spermatozoa tikus wistar (Rattus Norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Iis A.H. Silalahi ◽  
Grace L.A. Turalaki ◽  
Janette M. Rumbajan
Keyword(s):  
Free Radicals ◽  
Vitamin E ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Enzymatic Antioxidant ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Smoking can increase free radicals in sperm, which can damage sperm. Vitamin E as non-enzymatic antioxidant and zinc as enzymatic antioxidant that is effective in dealing with free radicals. The aim of this research was to look at the differences between the effects of vitamin E and zinc on spermatozoa quality of male wistar rats (Rattus norvegicus) after exposure to cigarette smoke. The research used a completely randomized experimental design. Samples were 9 male wistar rats (Rattus norvegicus) which were randomly divided into 3 groups. Each group was given exposure to cigarette smoke from 2 bars of cigarette/day, which treatment group (P1) also was given vitamin E 1,44 mg/day and treatment group (P2) also was given combination of vitamin E 1,44 mg/day and zinc 10 mg/day. The treatment was given for 50 days. The results of this research showed a significant difference in the motility and morphology of spermatozoa between the treatment group (P1) which received exposure the cigarette smoke and vitamin E and treatment group (P2) which received exposure to cigarette smoke and combination of vitamin E and zinc (p<0,05) compared with the group which received exposure only cigarette smoke without vitamin E or zinc. The motility of spermatozoa treatment group (P2) which received exposure the cigarette smoke and combination vitamin E and zinc was found significant difference with the treatment group (P1) which received exposure the cigarette smoke and vitamin E (p <0,05). Conclusion: The conclusion from this research showed that there is difference between spermatozoa quality especially motility and morphology of group treated with vitamin E and combination of vitamin E and zinc after exposure to cigarette smoke, with combination of vitamin E and zinc had an higher average on spermatozoa quality especially motility.Keywords: vitamin E, zinc, cigarette smoke, quality of spermatozoa Abstrak: Merokok dapat menyebabkan peningkatan radikal bebas yang dapat merusak sperma. Vitamin E sebagai antioksidan non enzimatis dan zink sebagai antioksidan enzimatis merupakan antioksidan yang efektif dalam mengatasi radikal bebas. Tujuan penelitian ini adalah untuk melihat perbedaan efek antara pemberian vitamin E dan kombinasi vitamin E dengan zink terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) jantan setelah diberi paparan asap rokok. Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak legkap. Sampel yang digunakan adalah 9 tikus wistar (Rattus norvegicus) jantan yang dibagi secara acak menjadi 3 kelompok. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi vitamin E 1,44 mg/hari, dan kelompok perlakuan (P2) diberi kombinasi vitamin E 1,44 mg/hari dan zink 10 mg/hari. Perlakuan diberikan selama 50 hari. Hasil penelitian menunjukkan adanya perbedaan signifikan pada motilitas dan morfologi spermatozoa kelompok perlakuan (P1) dengan perlakuan paparan asap rokok dan vitamin E dan kelompok perlakuan (P2) dengan perlakuan paparan asap rokok dan kombinasi vitamin E dengan zink dibandingkan dengan kelompok control (P0) yang hanya mendapat paparan asap rokok (p<0,05). Motilitas spermatozoa kelompok perlakuan (P2) didapati hasil yang berbeda signifikan dibandingkan dengan kelompok perlakuan (P1) (p<0,05). Simpulan: Kesimpulan dari penelitian ini didapatkan bahwa terjadi perbedaan signifikan kualitas spermatozoa yaitu pada motilitas dan morfologi antara pemberian tunggal vitamin E dan kombinasi vitamin E dengan zink setelah pemaparan asap rokok, dengan kombinasi vitamin E dan zink memiliki rata-rata kualitas spermatozoa lebih tinggi khususnya motilitas.Kata kunci: vitamin E, zink, asap rokok, kualitas spermatozoa

scholarly journals Protective role of Moringa oleifera leaf-based diet on protein- energy malnutrition induced skeletal muscle degeneration

2017 ◽  
Vol 3 (2) ◽  
pp. 54 ◽  
Author(s):  
Clement O. Bewaji ◽  
Muinat Olayinka Lambe
Keyword(s):  
Skeletal Muscle ◽  
Vitamin E ◽  
Protein Energy Malnutrition ◽  
Muscle Growth ◽  
Antioxidant Properties ◽  
Protective Role ◽  
Calcium Atpase ◽  
Disuse Atrophy ◽  
Muscle Degeneration ◽  
Negative Effects

<p class="abstract"><strong>Background:</strong> Sequel to the diverse diseases resulting in muscle mass degeneration and its key role in the prognosis of the diseases. For instance, currently, only resistance exercise can be used to promote recovery of mass/strength following disuse atrophy. But in contrast, many patients are unable or unwilling to exercise at a sufficient intensity to promote muscle growth. It would therefore be of great advantage to develop natural compounds that counteract the negative effects of skeletal muscle degenerative diseases especially in the form of therapeutic feed). The present study was aimed at elucidating the mechanism by which <em>M. oleifera</em> ameliorates skeletal muscle degeneration caused by malnutrition.</p><p class="abstract"><strong>Methods:</strong> The experimental animals were malnourished with low protein iso-caloric diets for four weeks after which they were treated with 25% <em>M. oleifera</em> leaf – based diet, vitamin E supplemented feed and soy bean based- diet for another four weeks<span lang="EN-IN">. </span><span lang="EN-IN"> </span></p><p class="abstract"><strong>Results:</strong> There was a significant reduction in the activity of calcium ATPase in the skeletal muscle of animals induced with skeletal muscle degeneration. However, this activity was significantly increased following treatments with the most significant increase observed in the skeletal muscle of animals treated with <em>M. Oleifera</em> leaf based diet followed by those fed with vitamin E supplemented diet.</p><p class="abstract"><strong>Conclusions:</strong> In summary, the study revealed that the mechanism by which <em>M. oleifera</em> leaf corrects muscle degeneration caused by PEM might be by increasing calcium ATPase activity and/ or its synthesis and by preventing oxidative stress due to its antioxidant properties<span lang="EN-IN">. </span></p>

scholarly journals Pulmonary Protective Effects of Propofol on Cisplatin-Induced Oxidative Damage in Male Rats

2020 ◽  
Vol 1 (2) ◽  
pp. 72-75
Author(s):  
Sajjad Makhdoomi ◽  
Akram Oftade ◽  
Sodabe Khodabandehlou ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Lung Tissue ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Protective Effects ◽  
Protected Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Pulmonary Damage ◽  
Male Wistar Rats

Background: The present study was performed to investigate the protective effects of propofol against cisplatin-induced pulmonary toxicity in rats. Methods: A total of 20 male Wistar rats weighing 180-250 g were divided into four groups of control, the cisplatin-intoxicated group intraperitoneally (IP) injected with cisplatin (7 mg/kg/d for a week), the propofol group (10 mg/kg/d, IP), and the protected group receiving propofol (10 mg/kg/d, IP) poisoned by cisplatin. Then, the biomarkers of total antioxidant capacity (TAC), catalase (CAT) activity, and lipid peroxidation (LPO) were measured in homogeneous lung tissues. Results: The data revealed the evidence of oxidative stress in the lung tissue of cisplatin-intoxicated rats as indicated by an increase in the level of LPO compared with propofol and protected groups (P<0.05). Moreover, TAC decreased in the cisplatin group while it increased in the propofol group compared to cisplatin and protected groups (P<0.05). No significant difference was observed between the groups regarding CAT (P>0.05). Protection with propofol ameliorated the oxidative stress induced by cisplatin in the lung tissue because of the reduction of LPO. Conclusion: According to these results, it seems that propofol provides a remarkable protection against cisplatin-induced oxidative pulmonary damage mediated by its antioxidant properties.

The impact of Crocus sativus stigma against methotrexate-induced liver toxicity in rats

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Reyhane Hoshyar ◽  
Ahmadreza Sebzari ◽  
Mohadeseh Balforoush ◽  
Masoomeh Valavi ◽  
Mehran Hosseini
Keyword(s):  
Plasma Levels ◽  
Liver Toxicity ◽  
Post Treatment ◽  
Crocus Sativus ◽  
Protective Effects ◽  
Sod Activity ◽  
Morphological Alterations ◽  
Liver Histopathology ◽  
Male Wistar Rats ◽  
The Impact

AbstractBackgroundThe adverse effects of methotrexate (MTX) mainly hepatotoxicity restrict its clinical use. This study was designed to investigate the protective effects of saffron (Crocus sativus) (CS) extract on MTX-induced hepatotoxicity.MethodsTwenty-eight male Wistar rats randomly divided into four equal groups. Except for control, all groups received a single intraperitoneal (i.p.) injection of MTX on the 3rd day of study. The CS extract was given (80 mg/kg i.p.) to rats 3 days before MTX and continued for the next 7 days (Pre&Post-CS group) or administrated after MTX injection and lasted for 7 days (Post-CS group). On the 11th day, all rats were sacrificed and their plasma levels of liver enzymes including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined. Also, liver histopathology and hepatic levels of malondialdehyde (MDA), nitric oxide (NO) and super oxidase dismutase (SOD) were evaluated.ResultsThe results showed that MTX significantly incremented plasma levels of AST, ALT, ALP and LDH (all p<0.001) and hepatic MDA and NO levels; whereas, decreased SOD activity. Histological alterations such as early fatty changes were evident in the MTX group. Administration of CS extract at both methods could ameliorate liver enzyme elevation, oxidative/nitrosative stresses and morphological alterations of the liver. Pre-and-post treatment with CS extract showed better protective effects than only post-treatment.ConclusionThe present findings provide showing CS could effectively alleviate MTX-induced hepatotoxicity in rats. Further investigations are recommended to determine the exact mechanisms underlying the hepatoprotective potential of saffron.

Red wine extract, resveratrol, on maintenance of organ function following trauma-hemorrhage

2012 ◽  
Vol 2 (10) ◽  
pp. 351
Author(s):  
Fu-Chao Liu ◽  
Huang-Ping Yu
Keyword(s):  
Intracellular Signaling ◽  
Red Wine ◽  
Antioxidant Properties ◽  
Neutrophil Function ◽  
Protective Effects ◽  
Organ Function ◽  
Mitogen Activated Protein ◽  
Ros Formation ◽  
Anti Inflammatory ◽  
And Control

Resveratrol, is a polyphenol that can be extracted from grapes and red wine, possess potential anti-inflammatory effects, which would result in the reduction of cytokine production, the alteration of the expression of adhesion molecule molecules, and the inhibition of neutrophil function. Resveratrol might also act as an antioxidant, anti-aging, and control of cell cycle and apoptosis. Resveratrol has been shown to have protective effects for patients in shock-like states. Such protective phenomenon is reported to be implicated in a variety of intracellular signaling pathways including the regulation of the mitogen-activated protein kinases (MAPK)/ hemeoxygenase-1 (HO-1) pathway, activates estrogen receptor (ER), and the mediation of pro-inflammatory cytokines, reactive oxygen species (ROS) formation and reactive. Moreover, through anti-inflammatory effects and antioxidant properties, the resveratrol is believed to maintain organ function following trauma-hemorrhage.Key words: resveratrol, anti-inflammatory, trauma-hemorrhage.

Anti-toxicant Properties of Saffron and Relevance to Protection from Toxins and Drugs

2020 ◽  
Vol 16 (3) ◽  
pp. 265-283
Author(s):  
Kyriaki Hatziagapiou ◽  
George I. Lambrou
Keyword(s):  
Redox Reactions ◽  
Case Reports ◽  
Meta Analysis ◽  
Reactive Oxygen ◽  
Crocus Sativus ◽  
Protective Effects ◽  
Nitrogen Species ◽  
Eligibility Criteria ◽  
Crocus Sativus L

Background: Reactive oxygen species and reactive nitrogen species, which are collectively called reactive oxygen nitrogen species, are inevitable by-products of cellular metabolic redox reactions, such as oxidative phosphorylation in the mitochondrial respiratory chain, phagocytosis, reactions of biotransformation of exogenous and endogenous substrata in endoplasmic reticulum, eicosanoid synthesis, and redox reactions in the presence of metal with variable valence. Among medicinal plants there is a growing interest in Crocus sativus L. It is a perennial, stemless herb, belonging to Iridaceae family, cultivated in various countries such as Greece, Italy, Spain, Israel, Morocco, Turkey, Iran, India, China, Egypt and Mexico. Objective: The present study aims to address the anti-toxicant role of Crocus sativus L. in the cases of toxin and drug toxification. Materials and Methods: An electronic literature search was conducted by the two authors from 1993 to August 2017. Original articles and systematic reviews (with or without meta-analysis), as well as case reports were selected. Titles and abstracts of papers were screened by a third reviewer to determine whether they met the eligibility criteria, and full texts of the selected articles were retrieved. Results: The authors focused on literature concerning the role of Crocus Sativus L. as an anti-toxicant agent. Literature review showed that Saffron is a potent anti-toxicant agent with a plethora of applications ranging from anti-oxidant properties, to chemotherapy protective effects. Conclusion: Literature findings represented in current review herald promising results for using Crocus Sativus L. and/or its active constituents as anti-toxicant, chemotherapy-induced protection and toxin protection.

scholarly journals Sustainable Processing of Floral Bio-Residues of Saffron (Crocus sativus L.) for Valuable Biorefinery Products

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 523
Author(s):  
Stefania Stelluti ◽  
Matteo Caser ◽  
Sonia Demasi ◽  
Valentina Scariot
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Antioxidant Properties ◽  
Crocus Sativus ◽  
Extraction Technique ◽  
Ultrasound Assisted Extraction ◽  
Average Value ◽  
Assisted Extraction ◽  
High Yields ◽  
Crocus Sativus L

Tepals constitute the most abundant bio-residues of saffron (Crocus sativus L.). As they are a natural source of polyphenols with antioxidant properties, they could be processed to generate valuable biorefinery products for applications in the pharmaceutical, cosmetic, and food industries, becoming a new source of income while reducing bio-waste. Proper storage of by-products is important in biorefining and dehydration is widely used in the herb sector, especially for highly perishable harvested flowers. This study aimed to deepen the phytochemical composition of dried saffron tepals and to investigate whether this was influenced by the extraction technique. In particular, the conventional maceration was compared with the Ultrasound Assisted Extraction (UAE), using different solvents (water and three methanol concentrations, i.e., 20%, 50%, and 80%). Compared to the spice, the dried saffron tepals showed a lower content of total phenolics (average value 1127.94 ± 32.34 mg GAE 100 g−1 DW) and anthocyanins (up to 413.30 ± 137.16 mg G3G 100 g−1 DW), but a higher antioxidant activity, which was measured through the FRAP, ABTS, and DPPH assays. The HPLC-DAD analysis detected some phenolic compounds (i.e., ferulic acid, isoquercitrin, and quercitrin) not previously found in fresh saffron tepals. Vitamin C, already discovered in the spice, was interestingly detected also in dried tepals. Regarding the extraction technique, in most cases, UAE with safer solvents (i.e., water or low percentage of methanol) showed results of phenolic compounds and vitamin C similar to maceration, allowing an improvement in extractions by halving the time. Thus, this study demonstrated that saffron tepals can be dried maintaining their quality and that green extractions can be adopted to obtain high yields of valuable antioxidant phytochemicals, meeting the requirement for a sustainable biorefining.

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