scholarly journals Content of lipid peroxidation products in pig meat at industrial and domestic slaughter

2020 ◽  
Vol 22 (99) ◽  
pp. 69-74
Author(s):  
I. Yu. Stronskyi ◽  
M. R. Simonov ◽  
Yu. S. Stronskyi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Properties ◽  
Lipid Hydroperoxides ◽  
Secondary Products ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
Organoleptic Characteristics ◽  
Pig Meat ◽  
Meat Samples ◽  
Pig Carcasses

The nutritional value of meat, its organoleptic characteristics and therefore the shelf life depend on the content of lipid peroxidation products in it. Intermediates of peroxidation can have a toxic effect on the human body and are one of the main factors of non-microbial degradation of meat and various products. Based on this, the aim of this work was to study the content of lipid peroxidation products in pig meat of different origins. Furthermore, the aim was to establish the level of primary and secondary products of lipid peroxidation in meat, selected from different parts of the carcass. Meat samples were taken immediately after the pigs were slaughtered. A total of 3 meat samples were taken from 10 pig carcasses. One sample of meat was taken from each of the carcasses from the cervical, dorsal and femoral parts. The first 5 pigs were kept in industrial conditions and were slaughtered within the industrial slaughterhouse, and the second 5 were kept and slaughtered in personal farms. The results of the research showed that the highest level of primary and secondary products of lipid peroxidation is registered in the meat obtained from the dorsal part of pork carcass, which is primarily due to its fat-acid composition. In meat selected from the cervical and femoral parts, the level of the studied indicators is probably lower. During storage, lipid peroxidation activity increases from the first to the fourth day and decreases slightly until the seventh. The increase in the level of all studied indicators on the fourth day of meat storage is due to the fact that natural components contained in muscle tissue, such as ferrum, myoglobin, hydrogen peroxide and ascorbic acid can cause lipid oxidation, acting as catalysts or promoting the formation of reactive oxygen species. In the case of domestic keeping and slaughter, pig meat was found to have significantly higher concentrations of diene conjugates, lipid hydroperoxides and TBA-active products compared to industrial. The main prerequisite for this is an unbalanced diet, especially in terms of the content of substances that have antioxidant properties.

scholarly journals Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

2019 ◽  
Vol 10 (1) ◽  
pp. 87-91
Author(s):  
A. R. Shcherbatyy ◽  
L. G. Slivinska ◽  
B. V. Gutyj ◽  
V. L. Fedorovych ◽  
B. O. Lukashchuk
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Immune Complexes ◽  
Positive Influence ◽  
Lysozyme Activity ◽  
The State ◽  
Circulating Immune Complexes ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

scholarly journals OXIDATIVE AND ANTIOXIDANT PROCESSES IN THE FOCUS OF THE SKIN EXPOSED TO LOCAL ULTRAVIOLET IRRADIATION

2019 ◽  
Vol 19 (3) ◽  
pp. 142-145
Author(s):  
S. I. Myronchenko
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Ultraviolet Radiation ◽  
Ultraviolet Irradiation ◽  
Guinea Pigs ◽  
Control Group ◽  
Secondary Products ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products

The purpose of this study was to investigate the effect of local ultraviolet radiation on the content of lipid peroxidation products, lipofuscin, and the functioning of antioxidant enzymes in the skin of guinea pigs. Materials and methods. The studies were performed on 24 albino guinea pigs exposed to a single local ultraviolet radiation. The control group was made up of intact animals. The content of primary (diene conjugates) and secondary (TAC-active products) lipid peroxidation products, the activity of antioxidant enzymes (superoxide dismutase and catalase), and the content of lipofuscin were assessed in the skin in 2 hours, 4 hours, and on the 3rd and 8th day following the UV irradiation. Results. Under the influence of local ultraviolet radiation, all guinea pigs develop erythema (its peak is observed in 4 hours, and on the 3rd day), which disappears on the 8th day. There is a sharp enhancement of lipid peroxidation processes due to the accumulation of lipid peroxidation primary and secondary products in the skin of guinea pigs in all periods of the experiment. In parallel with the increase in lipid peroxidation products, there is a decrease in the activity of antioxidant enzymes (catalase and superoxide dismutase) in the focus of radiation. The content of lipofuscin in the skin progressively increases throughout the study period. Conclusions. The early erythema period following the local ultraviolet irradiation in the skin of guinea pigs, is characterized by increased content of diene conjugates and TBA-active products (in 2, 4 hours, on the 3rd day); decreased catalase activity and increased lipofuscin concentration (on the 3rd day after irradiation). The early post-erythema period (8th day) following the local ultraviolet irradiation is characterized by the accumulation of diene conjugates, TBA-active products and lipofuscin against the background of a decrease in the activity of catalase and superoxide dismutase in the skin of guinea pigs.

CHANGE IN THE QUANTITATIVE CONTENT OF DIENE CONJUGATES IN THE ORAL FLUID IN RATS UNDER THE EXPOSURE OF TETRAMETHYLTHIURAM DISULFIDE

2020 ◽  
Author(s):  
Лилия Александровна Ячменева ◽  
Владимир Анатольевич Королев ◽  
Елена Викторовна Фелькер ◽  
Иван Владимирович Королев
Keyword(s):  
Lipid Peroxidation ◽  
Toxic Effect ◽  
Oral Fluid ◽  
Diene Conjugates ◽  
Tetramethylthiuram Disulfide ◽  
Lipid Peroxidation Products ◽  
Quantitative Content

Целью исследования было установить уровень диеновых конъюгатов в ротовой жидкости при интоксикации тетраметилтиурамдисульфидом. В результате было установлено, что на 28 сутки был выявлен наибольший токсический эффект ТМДТ, который превысил контрольные значения в 4 раза (400%). Концентрация ДК на 7, 14, 21 сутки возросла на 158,3%, 225,0%, 258,3% соответственно. Полученные результаты говорят о накоплении продуктов перекисного окисления липидов диеновых конъюгатов с увеличением сроков пестицидной интоксикации. The aim of the study was to determine the level of diene conjugates in the oral fluid during tetramethylthiuram disulfide intoxication. As a result, it was found that on day 28, the greatest toxic effect of TMDT was detected, which exceeded the control values by 4 times (400%).. The concentration of DC on days 7, 14, and 21 increased by 158.3%, 225.0%, and 258.3%, respectively. The results obtained indicate the accumulation of lipid peroxidation products of diene conjugates with an increase in the duration of pesticide intoxication.

Multivitamin and mineral supplementation in 1,2-dimethylhydrazine induced experimental colon carcinogenesis and evaluation of free radical status, antioxidant potential, and incidence of ACF

2012 ◽  
Vol 90 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Albert Baskar Arul ◽  
Ignacimuthu Savarimuthu ◽  
Mohammed A. Alsaif ◽  
Khalid S. Al Numair
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Colon Carcinogenesis ◽  
Aberrant Crypt Foci ◽  
Lipid Hydroperoxides ◽  
Entire Study ◽  
Lipid Peroxidation Products ◽  
Histopathological Alterations ◽  
Mineral Supplementation

This study was performed to determine the chemopreventive and antioxidant status of multivitamin and mineral (0.01% in drinking water, ad libitum) supplements in 1,2-dimethylhydrazine (DMH)-induced experimental colon carcinogenesis. Experimental colon carcinogenesis was induced in male albino Wistar rats by injecting DMH (20 mg·(kg body mass)–1) once weekly for 15 consecutive weeks, and administering a multivitamin supplement in 3 regimes (initiation, post-initiation, and entire experimental period) for 32 weeks. We studied lipid peroxidation products (thiobarbituric acid reactive substances, lipid hydroperoxides, conjugated dienes) in the circulation and in the tissues, antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and non-enzymatic antioxidant-reduced glutathione) of the tissues, aberrant crypt foci (ACF), and histopathological alterations. DMH-induced rats had an increase in lipid peroxidation products and a lower antioxidant status compared with control animals. Multivitamin and mineral supplementation during the initiation, post-initiation, and the entire study period significantly decreased the levels of lipid peroxidation products in circulation and colonic tissues, significantly elevated the activities of the antioxidant enzymes and reduced glutathione to near normalcy in DMH-induced rats. The incidence of ACF was reduced to 84.1% in rats supplemented with multivitamin and minerals for the entire study and prevented the colonic tissue from histopathological alterations induced by DMH.

scholarly journals The study of influence of the extracts obtained from prunus domestica fruits on lipid peroxydation and antioxidant system indicators in liver

2019 ◽  
pp. 85-93
Author(s):  
Bashar Jabbar Ali Al-Sahlanee ◽  
I. V. Senyuk ◽  
L. V. Lenchyk ◽  
T. V. Upyr
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Effective Dose ◽  
Rat Liver ◽  
Antioxidant System ◽  
Antioxidant Properties ◽  
Diene Conjugates ◽  
Reference Drug ◽  
Wide Range

The study of pharmacological properties and experimental substantiation of application possibilities of the plum fruits extracts in the treatment of the gastrointestinal tract and hepatobiliary system diseases is relevant, because plum is source of phenolic compounds with a wide range of action and is used in folk medicine for treating diseases of digestive system. The aim of the present work was an experimental study of anti free radical activity by lipid peroxidation indicators and antioxidant properties by the antioxidant system (AOS) indicators of two Plum extracts, detecting the most effective extract and determining its effective dose. The objects of research were extracts obtained from the fresh plum fruits: plum extract containing fibers (PEF) and extract containing plum polysaccharide complex (PEPC). Research methods: pharmacological (modeling of alcoholic damage to rat liver), biochemical (determination of the level of diene conjugates, TBA-AP, lipid hydroxyperoxides, reduced glutathione and α-tocopherol in rat liver homogenate) and statistical (using the program «Statistica 6.0», Student’s t-test). Experimental data on the study of the anti free radical properties of the plum extracts showed inhibitory effects on lipid peroxidation, reducing the content of diene conjugates, TBAs, and lipid hydroxyperoxides under conditions of alcoholic liver damage. Regarding the effect of the investigated extracts on the markers of the antioxidant system of hepatocytes, there was an increase in the content of reducing glutathione and α-tocopherol on the alcoholic hepatitis model. The fiber-containing extract (PEF) was the most effective in normalizing the functional state of the liver at a dose of 200 mg/kg. Thus, it was determined that an effective dose of extract with fibers (PEF) was 200 mg/kg. The investigated effects of PEF in 200 mg/kg exceeded the effects of PEPS at the tested doses and were at the level of activity of reference drug «Silibor» in the dose of 25 mg/kg. Anti free radical and antioxidant properties of the PEF are probably related to its chemical composition namely phenolic compounds (anthocyanins and hydroxycinnamic acids). Considering the results of screening, the most promising subject for further in-depth pharmacological study is plum extract PEF at dose 200 mg/kg.

scholarly journals CONTENT OF PRIMARY AND SECONDARY LIPID PEROXIDATION PRODUCTS IN SUBCELLULAR FRACTIONS OF CARDIOMYOCYTES DURING MYOCARDIAL INFARCTION IN RATS IN AN EXPERIMENT AND THEIR CORRECTION BY TRANSPLANTATION OF MESENCHYMAL STEM CELLS

2021 ◽  
Vol 11 (4) ◽  
pp. 89-92
Author(s):  
Vyacheslav Mykhaylichenko ◽  
Andrey Pilipchuk ◽  
Dmitry Parshin ◽  
Yuri Kostyamin
Keyword(s):  
Myocardial Infarction ◽  
Lipid Peroxidation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Subcellular Fractions ◽  
Control Group ◽  
Diene Conjugates ◽  
Mesenchymal Stem Cell Transplantation ◽  
Lipid Peroxidation Products ◽  
Group I

Experimental modeling of myocardial infarction in rats was carried out by ligation of the anterior intergastric artery after the first division. There were 3 groups of 20 animals each: control group I — to verify normal parameters, group II — a model of myocardial infarction, and group III — animals which, after modeling myocardial infarction, underwent transplantation of mesenchymal stem cells. The level of lipid peroxidation products — diene conjugates and malondialdehyde — was studied by spectrophotometry. Comparison of the content and their ratio in the cytoplasm and mitochondria of myocardiocytes was carried out. It turned out that transplantation of mesenchymal stem cells significantly levels the activation of lipid peroxidation processes in subcellular fractions of cardiomyocytes, which is accompanied by a decrease in the primary and secondary products of oxidative stress. The ratio of malondialdehyde to diene conjugates both in the cytoplasm and in the mitochondria of cardiomyocytes after transplantation returned to control values. This indicates the normalization of physiological processes with underlying ischemic heart damage. The results indicate the cytoprotective effect of mesenchymal stem cell transplantation and the preservation of a larger number of cell pools, compared with the control group of animals that did not receive any treatment.

scholarly journals The ratio of erythrocyte populations in the blood of puppies during postnatal oxidative stress

2019 ◽  
pp. 81-87
Author(s):  
M. Anfiorova ◽  
M. Broshkov ◽  
O. Danchuk
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Key Words ◽  
Schiff Bases ◽  
The Body ◽  
Peroxide Oxidation ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products ◽  
End Products ◽  
Conjugated Trienes

The article presents new scientifi c data on the ratio of erythrocyte populations in puppies' blood during postnatal oxidative stress. It was established that the number of erythrocytes in the blood of one-day-old puppies was 5.64 ± 0.12 T / l, and half of these cells belonged to the population of "mature", 40% - "young" and 10% - "old". In day-old puppies, the content of lipid peroxidation products in erythrocyte hemolysates is quite high, which indicates the development of postnatal oxidative stress. Thus, the content of diene conjugates, ketodienes and Schiff bases is respectively 1,406 ± 0,023 Conv. units, 0.676 ± 0.004 Conv. units and 0.135 ± 0.003 Conv. units. From one to fi ve days age of puppies the number of erythrocytes in the blooddecrease by 1.5 times (p<0.001), mainly due to the decrease in the number of "mature" cells (by 1.6 times; p<0.001). The intensity of peroxide oxidation in the body of puppies even a month after birth is relatively high, as indicated by the content of Schiff bases in the erythrocytes of the blood of these animals - 0.185 ± 0.002 Conv.units. The inverse correlations of the content of Schiff bases in erythrocyte hemolysate with their number in the bloodstream were found to be r = -0.83 (p<0.001). It should also be noted the direct correlation of the content of diene conjugates in the blood of puppies with the number of erythrocytes in their blood (r = 0,58 p <0,05), but the content of ketodienes and conjugated trienes is inversely related to the content of "young" erythroid cells in the puppies' blood (r = -0.57 p <0.05). Consequently, post-natal adaptation of puppies results in the replacement of fetal erythrocytes by postnatal cells, and this process is associated with the intensifi cation of peroxide oxidation and accumulation of the end products of peroxidationin the cells. This process isaccompanied by an acceleration of the process of "aging" of young forms of erythrocytes, as a result of which prerequisites for the development of anemiaare created. Key words: erythrocytes, oxidative stress, puppies, Schiff bases, ketodienes, diene conjugates.

Antioxidant properties of ferulic acid and the main results of their implementation in the industrial incubation of turkeys

2021 ◽  
Vol 1 (1) ◽  
pp. 76-84
Author(s):  
T. O. Azarnova ◽  
◽  
A. M. Rezvykh ◽  
V. I. Maksimov ◽  
I. I. Kochish ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ferulic Acid ◽  
Carbohydrate Metabolism ◽  
Old Age ◽  
Antioxidant Properties ◽  
Live Weight ◽  
The Body ◽  
Diene Conjugates ◽  
Turkey Poults ◽  
Experimental Group

The presented article presented data confirming the antioxidant properties of ferulic acid, which is associated with inhibition of the intensity of lipid peroxidation in the body of day-old turkeys, expressed in a decrease in the concentration of its cytotoxic products formed at different stages of the peroxide cascade: isolated double bonds (IDB) by 1,2 times, diene conjugates (DC) 1,6 times (p<0,001,.1,1), triene conjugates (TC) and oxodienic conjugates (ODC) 1,4 times, Schiff bases (SB) 12,02 times compared to the control. This helped to optimize the central metabolic processes, in particular carbohydrate metabolism. The paper analyzes these changes, presents and explains their relationship with the increase in quantitative and qualitative indicators of young turkeys at the early stages of ontogenesis. Thus, the superiority of the experimental group over the control of hatching turkey poults was 0,01% (p<6,66), the hatchability of eggs was 8,23% with a tendency to an increase in live weight of individuals of day-old age by 0,5%, the score according to the «Pasgar» scales and «Optistart» by 0,05 (p<1,0) and 0,001 points (p<XNUMX), respectively.

Immunomodulatory and Antioxidant Action of Selective Inhibitor of Arginase IInor-NOHA and Antihypoxants in Some Extreme Conditions

2015 ◽  
Vol 22 (3) ◽  
pp. 161-164
Author(s):  
Лосенок ◽  
P. Losenok ◽  
Наседкин ◽  
D. Nasedkin ◽  
Квачахия ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Selective Inhibitor ◽  
Control Group ◽  
Extreme Conditions ◽  
Secondary Products ◽  
Diene Conjugates ◽  
Pharmacological Agents ◽  
Combined Application ◽  
Arginase Ii

The study of the effects of a selective inhibitor of arginase II nor-NOHA and its combinations with Hy-poxenum, Kudesan, Cytoflavin on the functional activity of neutrophils, lipid peroxidation and content of stable metabolites of nitrogen in the blood serum of rats under the influence of some extreme conditions was carried out. It was found that under conditions of hypoxia that occurs in some extreme situations, there is a reduction of spontaneous and induced phagocytic and metabolic activity of neutrophils. Combined application of nor-NOHA, hypoxen and cytoflavin increases spontaneous PMA of neutrophils to values of the control group, but does not influence the values of NST Ind. The authors found that at hypoxia, there is an increase in the content of primary and secondary products of lipid peroxidation. Administration nor-NOHA and antihypoxants reduces the concentration of malondialdehyde at all combinations of drugs, and reduction of diene conjugates only in animals treated with nor-NOHA, hypoxen and cytoflavin. Total antioxidant activity increased as well, for all combinations of pharmacological agents, and catalase activity was increased combinations nor-NOHA + cytoflavin and nor-NOHA + hypoxen + cytoflavin. At hypoxia, there is a significant increase of stable NO metabolites in blood serum. Introduction nor-NOHA further increases their concentration, and at different combinations antigipoksantov administered in combination with an inhibitor of arginase II nor-NOHA marked reduction of NO metabolite to an animal in a state of hypoxia.

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