scholarly journals Skrining Fitokimia Dan Aktivitas Antimikroba Dari Tumbuhan Bangun-Bangun (Coleus Amboinicus Lour)

2019 ◽  
Vol 2 (1) ◽  
pp. 92-96
Author(s):  
Rosliana Lubis
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Inhibitory Zone ◽  
E Coli ◽  
Activity Test ◽  
In Vitro Measurements

Penelitian ini bertujuan untuk mengekstraksi, skrining fitokimia, dan analisa aktivitas mikroba dari daun bangun-bangun dengan menggunakan pelarut etil asetat 70% dan n-heksan. Metode ekstraksi menggunakan metode maserasi. Komposisi kimia senyawa aktif yang diuji terdiri dari alkaloid, flavonoid, terpenoid, tanin, dan saponin. Aktivtias antimikroba ekstrak etil asetat 70% dilakukan menggunakan metode pengukuran luas zona hambat. Bakteri uji yang digunakan terdiri dari E. Coli ATCC 25922 dan Pseudomonas aeruginos. Uji aktivitas bakteri dilakukan bersifat invitro. Pengukuran zona hambat menggunakan variasi konsentrasi ekstrak etil asetat 70% terdiri dari 0%, 15%, 30%, 45%, 60%, dan 75% (v/v). Hasil Penelitian :menunjukkan bahwa ekstrak etil asetat 70% daun Handeuleum secara kualitatif teridentifikasi memilki kandungan senyawa kimia alkaloid, Flavonoid, Terpenoid, Steroid, Tanin, dan Saponin. Aktivitas bakteri E. Coli ATCC 25922 dan Pseudomonas aeruginosa terhadap ekstrak didasarkan kepada pengukuran luas zona hambat yang terbentuk. Luas zona hambat yang terbentuk dari ekstrak etil asetat daun Handeuleum dengan range konsentrasi 0% s.d 75 % terdiri dari 9,50 mm – 21,2 mm dan 9,00 mm – 20,6 mm.   This study aimed to extract, phytochemically screen, and analyze the microbial activity of bangun-bangun leaves using 70% ethyl acetate and n-hexane solvents. The extraction method used was maceration method. The chemical composition of the active compounds tested consisted of alkaloids, flavonoids, terpenoids, tannins, and saponins. The antimicrobial activity of 70% ethyl acetate extract was carried out using the method of measuring the inhibitory zone. The bacteria used consisted of E. coli ATCC 25922 and Pseudomonas aeruginos. The bacterial activity test was in vitro. Measurements of inhibitory zones using variations in the concentration of 70% ethyl acetate extract consisted of 0%, 15%, 30%, 45%, 60% and 75% (v / v). The results showed that 70% of the ethyl acetate extract of Handeuleum leaves were qualitatively identified as containing alkaloid chemical compounds, Flavonoids, Terpenoids, Steroids, Tanins, and Saponins. The activities of E. coli bacteria ATCC 25922 and Pseudomonas aeruginosa towards the extracts were based on measurements of the area of ​​inhibition zone formed. The area of ​​inhibition zone formed from the ethyl acetate extract of Handeuleum leaves were with a concentration range of 0% to 75% consisting of 9.50 mm - 21.2 mm and 9.00 mm - 20.6 mm.

scholarly journals Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi

Biosfera ◽  
2017 ◽  
Vol 33 (3) ◽  
pp. 126
Author(s):  
Febri Nur Ngazizah ◽  
Nuraeni Ekowati ◽  
Aisyah Tri Septiana
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Zone ◽  
Ethyl Palmitate ◽  
E Coli ◽  
Significant Difference

Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

scholarly journals Study of Phytochemicals, Toxicity, Antibacterial Activity of Ethyl Acetate Leaf Extract Extract (Paperomiapellucida L)

2020 ◽  
Vol 3 (2) ◽  
pp. 74
Author(s):  
Ida Duma Riris ◽  
Tita Juwitaningsih ◽  
Destria Roza ◽  
Marini Damanik ◽  
Albinus Silalahi
Keyword(s):  
Antibacterial Activity ◽  
Rural Communities ◽  
Herbal Medicine ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Inhibitory Zone ◽  
E Coli ◽  
Activity Test

Suruhan Leaf (Paperomiapellucida L) is a weedy plant that is used by rural communities as herbal medicine. In this study, phytochemical tests have identified compounds contained in the ethyl acetate extract, then the toxicity test was carried out by the Brint Shrimp LethallytyTocycity (BSLT) method using Artemia Salina Leach shrimp larvae, an antibacterial activity test for bacteria: Bacillus cereus (ATCC) 11778), S. aureus (ATCC 25923), E. coli (ATCC 25922), S. saprophytic (ATCC 49907), Catsobacterfrendi (ATCC 18090), P.acne (ATCC 27853), Epidermis (ATCC 12228) with diffusion methods, various of concentration 1; 5; 10; 25% as control used chloramphenicol 30 mcg, the highest inhibitory zone at a concentration of 25% showed a strong antibacterial succession of 20.7; 11.1; 29.7; 15; 13.5; 10; 17.3 mm. The results of the identification of compounds in ethyl acetate extract are alkaloids, flavonoids, steroids, saponins, and tannins. The toxicity of LC50 is 71.28 ppm (toxic).

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.)

2017 ◽  
Vol 4 (2) ◽  
pp. 172
Author(s):  
Srikandi Srikandi ◽  
I.G.A. Manik Widhyastini
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Solvent Concentration ◽  
Garcinia Mangostana ◽  
A Value ◽  
Hexane Extracts

Antibacterial Extract of Mangosteen (Garcinia mangostana L.)Fruit Shell           The skin of the mangosteen fruit is extracted with n-hexane and ethyl acetate to determine the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27 853. Results showed that n-hexane extract gave inhibition zone larger than the ethyl acetate extract on all concentrations . Extract n-hexane has a value of MIC against S. aureus ATCC bacterial test 25923 62.5 mg / ml while the ethyl acetate extract of 125 mg / ml . N- hexane extracts had MIC values of the test bacteria P.aeuroginosa ATCC 27 853 was 125 mg / ml , and while the ethyl acetate extract had a MIC value of 500 mg / ml . Treatment of solvent, concentration and interaction between the solvent and concentration significantly affected the test bacteria ATCC 25923 S. aureus at the level of 5 %, the highest interaction N-Hexane solvent with a concentration of 15,625 mg / ml and was not significantly different interactions with the concentration of 31.25 mg/ml and 125 mg/ml. Treatment solvent and concentration significantly while the interaction between the solvent and the concentration has no effect on the test bacteria P.aeuroginosa ATCC 27 853 at 5% level .Keywords: Garcinia mangostana L., Staphylococcus aureus and Pseudomonas aeruginosa ABSTRAK           Kulit buah manggis diekstrak dengan n-heksan dan etil asetat   untuk mengetahui Konsentrasi Hambat Minimum (KHTM) terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aeruginosa ATCC 27853. Hasil penelitian menunjukkan bahwa ekstrak n-heksana  memberikan zona hambatan lebih besar dibandingkan dengan ekstrak etil asetat pada semua konsentrasi. Ekstrak n-heksana  memiliki nilai kadar hambat minimal (KHM) terhadap bakteri uji S. aureus ATCC 25923  62,5 mg/ml sedangkan ekstrak etil asetat 125 mg/ml.   Ekstrak n-heksana memiliki nilai KHM   terhadap bakteri uji P.aeuroginosa  ATCC 27853 adalah 125 mg/ml dan  sedangkan ekstrak etil asetat memiliki nilai KHM  500 mg/ml. Perlakuan pelarut, konsentrasi dan interaksi antara pelarut dan konsentrasi  berpengaruh nyata terhadap bakteri uji S. aureus ATCC 25923 pada taraf 5%, interaksi tertinggi yaitu pelarut N-Heksan dengan konsentrasi 15,625mg/ml dan interaksi ini tidak berbeda nyata dengan konsentrasi 31,25 mg/ml dan 125 mg/ml. Perlakuan pelarut dan konsentrasi  berpengaruh nyata sedangkan interaksi antara pelarut dan konsentrasi  tidak berpengaruh terhadap bakteri uji P.aeuroginosa  ATCC 27853 pada taraf 5%. Kata kunci:  Garcinia mangostana L., Staphylococcus aureus dan Pseudomonas aeruginosa

scholarly journals Antibacterial Activity of Floral Petals of Some Angiosperms

2019 ◽  
pp. 1-5 ◽  
Author(s):  
L. Rajanna ◽  
N. Santhosh Kumar ◽  
N. S. Suresha ◽  
S. Lavanya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Assay

The in vitro antibacterial assay was carried out against both Gram positive (B. cerus and S. aureus) and Gram negative (E. coli and K. pneumoniae) bacteria. Floral petals of 20 different species of plants were collected and tested for antibacterial activity. The result showed that the petals were active against both Gram positive and Gram negative. Out of 20 floral petals tested, 19 floral petals exhibited antibacterial activity against selected bacterial strains. The minimal inhibitory zone of floral petal discs against human pathogenic bacteria varies from 2 – 6 mm. Rosa carolina and Ruellia tuberosa showed significance inhibition zone for all the bacterial strains while Lantana camara does not show inhibition zone for any of these pathogenic bacteria.

scholarly journals Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

2021 ◽  
Vol 21 (1) ◽  
pp. 281
Author(s):  
Muhammad Zaidan Tsani Ariandi ◽  
Meiskha Bahar ◽  
Hany Yusmaini ◽  
Fajriati Zulfa ◽  
Cut Fauziah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
P Value ◽  
E Coli ◽  
Botanical Gardens

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.  

scholarly journals Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

2020 ◽  
Vol 11 (1) ◽  
pp. 52-60
Author(s):  
Sanusi Ibrahim ◽  
Suryati Suryati ◽  
Enda Desriansyah Aziz
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Cytotoxicity Activity ◽  
E Coli ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Ethyl Acetate Extracts

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

scholarly journals Analisa Potensi Ekstrak Daun Kari (Murraya koenigii L. Spreng)

2019 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Mustanir Mustanir ◽  
Tara Rizki Al-Qarana ◽  
Hilda Gusvianna ◽  
Nurdin Saidi
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Murraya Koenigii ◽  
E Coli ◽  
Ic50 Values ◽  
Hexane Extracts ◽  
Inhibition Zones

Daun M. koenigii sangat umum digunakan sebagai rempah dalam berbagai masakan di Aceh, namun pemahaman masyarakat terhadap pentingnya daun M. koenigii perlu ditingkatkan agar pemanfaatannya dapat ditingkatkan. Dalam penelitian ini dilakukan uji aktivitas antibakterial daun M. koenigii terhadap bakteri E. coli dan S. aureus serta uji aktivitas antioksidan terhadap radikal bebas DPPH (1,1-Diphenyl-2-pycrilhydrazil). Uji antibakterial dilakukan dengan metode difusi cakram, dan hasilnya ekstrak etil asetat menghasilkan zona hambat yang lebih besar dibandingkan ekstrak metanol dan n-heksana. Zona hambat yang dihasilkan pada konsentrasi 1, 5 dan 10 % secara berturut-turut adalah 8,7; 7,7 dan 6,7 mm terhadap E. coli, dan 12,8 ;10,7 dan 8,0 mm terhadap S. aureus. Ekstrak etil asetat memiliki aktivitas antioksidan paling kuat dibandingkan dengan ekstrak metanol dan nheksana secara berturut-turut berdasarkan nilai IC50 yaitu 23; 50,54; dan 64,70 ppm. Berdasarkan kromatografi kolom diperoleh 8 (A-H) subfraksi dan subfraksi G menunjukan aktifitas antioksidan yang paling kuat sebesar 14,41 ppm   Murraya koenigii (M. koenigi) leaves are very commonly used as spices in various cuisines in Aceh, but people's understanding of the importance of M. koenigi leaves needs to be improved, so it can be utilized better. In this study, the antibacterial activity of M. koenigii leaves was carried out against E. coli and S. aureus and antioxidant activity against DPPH free radicals (1,1- Diphenyl-2-pycrilhydrazil). Antibacterial tests were carried out using the disc diffusion method, and as a result, the ethyl acetate extract produced a larger inhibition zone than the methanol and n-hexane extracts. The inhibition zones produced at concentrations of 1, 5 and 10% were 8.7; 7.7 and 6.7 mm against E. coli, and 12.8; 10.7 and 8.0 mm against S. aureus. Ethyl acetate extract had the strongest antioxidant activity compared to methanol and nexexane extracts based on IC50 values, 23; 50; 54; and 64.70 ppm. Based on column chromatography obtained, 8 (A-H) subfraction and subfraction G showed the strongest antioxidant activity of 14.41 ppm.

scholarly journals Antibacterial Screening Activity of Several Sponges Against Staphylococcus aureus, Escherichia coli, Staphylococcus saprophyticus, dan Pseudomonas aeruginosa

2019 ◽  
Vol 7 (2) ◽  
pp. 455
Author(s):  
Deiske A. Sumilat
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Inhibition Zone ◽  
E Coli ◽  
Antibacterial Screening ◽  
Sponge Extract ◽  
Screening Activity

Sponge samples collected around Manado waters were obtained 30 species and their crude extracted have been tested in vitro for their activity in inhibiting bacterial growth. Based on the results of antibacterial screening in 30 sponge extracts, there were 23 sponge extracts which had bioactivity in inhibiting the growth of S. aureus, E. coli, S. saprophyticus and P. aeruginosa, Sponge extract No. 43 (of 30 sponge extracts tested) was the most active in inhibiting bacterial growth and had the widest inhibition zone diameter.Keywords: screening, sponge, crude extract, antibacterial, ManadoABSTRAKSampel spons dikoleksi di sekitar Perairan Manado sebanyak 30 jenis/spesies, dimana ekstrak kasarnya telah diuji secara in vitro aktivitasnya dalam menghambat pertumbuhan bakteri. Berdasarkan hasil skrining antibakteri pada 30 ekstrak spons didapatkan hasil ada 23 ekstrak spons yang mempunyai bioaktivitas dalam menghambat pertumbuhan bakteri S. aureus, E. coli, S. saprophyticus dan P. aeruginosa, Ekstrak spons No. 3 (dari 30 ekstrak spons yang diuji) adalah yang paling aktif dalam menghambat pertumbuhan bakteri dan memiliki diameter zona hambat yang paling lebar.Kata kunci: skrining, spons, ekstrak kasar, antibakteri, Manado

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