scholarly journals Aktivitas Hepatoprotektor Ekstrak Etanol Kulit Bawang Merah (Allium cepa L. Corium) Terhadap Mencit Jantan Yang Diinduksi Parasetamol

2018 ◽  
Vol 1 (3) ◽  
pp. 001-006
Author(s):  
Janet Bardos ◽  
Aminah Dalimunthe ◽  
Urip Harahap
Keyword(s):  
Allium Cepa ◽  
Liver Damage ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
High Dose ◽  
Reactive Metabolite ◽  
Allium Cepa L ◽  
Hepatoprotective Agent

Metabolit reaktif N-acetyl-para-benzoquinoneimine (NAPQI) akan dilepaskan setelah pemberian parasetamol dosis tinggi sehingga dapat merusak hati. Salah satu tumbuhan yang berpotensi sebagai hepatoproktektif adalah bawang merah (Allium cepa L.). Tujuan penelitian ini adalah untuk mengevaluasi aktivitas hepatoprotektor ekstrak etanol kulit bawang merah (EEKBM) terhadap mencit jantan yang diinduksi parasetamol. Enam kelompok perlakuan (n= 4) diberi Na CMC 0,5% sebagai kontrol negatif, EEKBM dosis 300, 450, dan 600 mg/kg bb, dan rutin 20 mg/kg bb sebagai kontrol positif selama 14 hari secara oral dan diikuti pemberian penginduksi hepatotoksik parasetamol 1 g/kg bb. Sampel darah dan hati dikumpulkan untuk pemeriksaan biokimia dan mikroskopik. Kelompok normal digunakan sebagai pembanding. Berdasarkan hasil penelitian menunjukkan bahwa kadar AST dan ALT dihambat oleh EEKBM dosis 300, 450, dan 600 mg/kg bb dan berbeda signifikan dengan kontrol negatif (p < 0,05) dan melindungi sel hati dari kerusakan. Dapat disimpulkan bahwa EEKBM dosis 600 mg/kg bb menunjukkan aktivitas hepatoprotektor.   The reactive metabolite, N-acetyl-para-benzoquinoneimine (NAPQI), could be released after administration of high dose paracetamol and induced liver damage. One of plant that has potential to be developed into hepatoprotective agent is Onion (Allium cepa L.). This research aimed to evaluate the hepatoprotective activity of onion ethanol extract (OEE) in paracetamol induced mice. There were six groups (n=4), consist of negative control which received Na CMC 0,5% , treatment group received OEE at doses of  300, 450, dan 600 mg/bw, and rutin at dose of 20 mg/bw was positive control for 14 days by oral adminitration and followed by paracetamol 1 g/bw to induce hepatotoxic. Blood sample and liver organ were collected for biochemistry and microscopic examinations. Normal control was served as comparison. The results showed that AST and ALT levels was inhibited by OEE at doses of 300, 450, dan 600 mg/kg bwand significantly different with negative control (p < 0,05) and prevented liver damage. It can be concluded that OEE at the dose of 600 mg/kg bwhas hepatoprotective activity.

scholarly journals HEPATOPROTECTIVE ACTIVITY FROM ETHANOL EXTRACT OF PUGUN TANO’S LEAVE (Curanga fel-terrae (Lour.) Merr.)

2017 ◽  
Vol 10 (11) ◽  
pp. 78
Author(s):  
Urip Harahap ◽  
Marianne Bastian ◽  
Sri Yuliasmi ◽  
Dadang Irfan Husori ◽  
Popi Patilaya ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Normal Group ◽  
Control Group ◽  
High Dose

Objective: The aim of this study is to observe the activity of ethanol extract of Curanga fel-terrae leave in preventing the damaged of liver which is induced by high dose of paracetamol. Methods: This research was conducted by using Wistar rat divided into 6 groups. Group 1 was the normal group. Group 2, 3, 4, 5 and 6 received CMC-Na 0.5% (negative control), C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg, catechin 2 mg/kg (positive control),respectively during 7 days continued and followed by given paracetamol dose of 2.5g/kg 8 hours after that. Hepatoprotective activity was carried out toward parameter of AST, ALT as well as histopathology of the liver. Results: The results showed that high-dose paracetamol dose of 2.5g/kg bw can cause liver damaged which can be seen by the increasing of the level of AST and ALT compared to the normal group (p<0.05). The usage of three doses of CFEEfor 7 days showed the prevention of the increasing of the level of AST and ALT compared to negative control group (p<0.05). Furthermore, the histopathology study revealed that the three doses of extract could protect the liver. Conclusion: The C. fel-terrae ethanolic extract (CFEE) at the doses of 125, 250 and 500 mg/kg bw which was given for 7 days can prevent the liver from the damage caused by high-dose of paracetamol. Keyword: Curanga fel-terrae (Lour.) Merr., paracetamol, liver, hepatoprotective   

scholarly journals Hepatoprotective activity of the ethanol extract of Curcuma heyneana rhizome on isoniazid and rifampin-induced liver injury rats

2020 ◽  
Vol 9 (4) ◽  
pp. 333-338
Author(s):  
Marianne Marianne ◽  
Urip Harahap ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Chintya Monica Thampati ◽  
Herman Syukur Harefa
Keyword(s):  
Liver Injury ◽  
Biochemical Parameters ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Alanine Transaminase ◽  
Control Group ◽  
Inhibition Concentration ◽  
Dpph Method ◽  
Hepatoprotective Agent

Introduction: Isoniazid (INH) and rifampin (RIF) are antituberculosis drugs that can induce injury in the liver. The purpose of this study was to investigate the antioxidant and hepatoprotective activities of the ethanol extract of Curcuma heyneana rhizome on liver injury in animal model induced by INH and RIF. Methods: Antioxidant activity was measured by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. All animals were divided into 7 groups. Liver injury was induced by using combination of INH at the dose of 50 mg/kg and RIF at the dose of 100 mg/kg. These drugs were administrated for 15 days along with extracts at doses of 5, 25, 125 or 625 mg/kg. Positive control group was given catechin. On the 16th day, the rats were sacrificed, blood and livers were collected for assessment of biochemical parameters like alanine transaminase (ALT) and aspartate transaminase (AST) and histological studies, respectively. Results: The ethanol extract strongly scavenged DPPH with inhibition concentration 50 (IC50) of 82.48 ppm. Administration of ethanol extract of C. heyneana rhizome at the dose of 25, 125 or 625 mg/kg significantly inhibited the elevation of AST and ALT (P<0.01). Moreover, the effects caused by administration of the extracts were similar to the effects caused by catechins. Various doses of the extract could effectively reduce tissue damage. Conclusion: This result suggests that ethanol extract of C. heyneana rhizome at the doses of 25, 125 and 625 mg/kg might be effective as hepatoprotective agent.

scholarly journals Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 35-40
Author(s):  
Yuandani ◽  
Silvia Mardaliza ◽  
Marianne
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepatoprotective Effect ◽  
Hepatic Tissue ◽  
High Dose ◽  
Male Mice ◽  
Mice Liver

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice  received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw .   Keywords: C. mangga, rhizomes, biochemistry parameters, histopathology

scholarly journals Potensi Ekstrak Etanol 96% Daun Salam (Syzigium polyanthum Wight. (Walp.)) terhadap Ekspresi p53 pada Sel Kanker HeLa Cell Lines

2020 ◽  
pp. 79-86
Author(s):  
Wahyu Hidayati ◽  
Landyyun Rahmawan Sjahid ◽  
Wahyu Ismalasari ◽  
Kusmardi Kusmardi
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
P53 Protein ◽  
Ethanol Extract ◽  
Suppressor Gene ◽  
Positive Control ◽  
Negative Control ◽  
High Dose ◽  
Hela Cell Lines ◽  
Post Hoc

Bay leaves (Syzigium polyanthum Wight. (Walp.) have been utilized as one of traditional medicines in Indonesian. However, the potency of the leaves for cancer treatment has not been well-explored. The aim of this study is to determine the expression of p53 tumor suppressor gene after administration of 96% ethanol extract of Bay leaves on HeLa cell lines. This study is begin by making extract using ethanol 96% as a solvent followed by phytochemical screening and extract evaluation of HeLa cells by immunohistochemistry. The observation of p53 protein expression is conducted using the Image J Profiler Plugin software 1.52a version. The H-score data is processed using the One-Way ANNOVA IBM SPSS 20 version program and continued with Post Hoc analysis using the Duncan test. The identification of 96% ethanol extract result showed that bay leaves contain flavonoids, alkaloids, saponins, phenols, terpenoids, and steroids. Based on the Post Hoc test results, the administration of 96% ethanol extract bay leaves with three dose variations (156 µg/ml, 234 µg/ml, 312 µg/ml) and negative control had significant differences, while the high dose (312 µg/ml) had not significant differences to positive control. The result showed that 96% ethanol extract of bay leaves by the dose of 312 ug/ml can inhibit the expression of mutant p53 protein in cervical cancer cells HeLa cell lines.

scholarly journals Hepatoprotective Activity of Ethanol Extract of Figs Leaves (Ficus carica L.) with SGOT & SGPT Parameters in Sprague Dawley Female Rats Induced by Paracetamol

2021 ◽  
Vol 7 (2) ◽  
pp. 110-119
Author(s):  
Muhammad Fariez Kurniawan ◽  
Herdita Nugraheny Kusuma Wardany
Keyword(s):  
Ethanol Extract ◽  
Test Dose ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Serum Glutamic Oxaloacetic Transaminase

Background: Long-term usage of paracetamol damages liver cells characterized by the increasing levels of Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT). Figs (Ficus carica L.) leaves contain high flavonoid compounds that able to act as hepatoprotector agents which inhibited the process of liver damage. Objectives: This study aims to determine the dose characteristics and hepatoprotective activity of figs (Ficus carica L.) leaves extract. Material and Methods: The hepatoprotective activity of figs extract (Ficus carica L.) was determined by measuring SGPT & SGOT levels in rat blood. 42 rats were prepared, divided into 7 groups: normal control group, negative control given paracetamol with a dose 40 mg/200 g BW, positive control given hepamax®, base control given Na CMC, and 3 test dose groups given figs leaves extract with a dose of 40 mg/200 g BW, 80 mg BW/200 g BW and 160 mg/200 g BW. The treatment was carried out for 14 consecutive days. Paracetamol was given for 14 days, while positive control, 3 test dose groups and base control were given on day 7 to day 14.  Rats blood samples were taken through the orbital sinuses on day 1, day 5, day 10 and day 14 after treatment began. SGPT & SGOT levels were determined using spectrophotometry with analytical methods using specific SGPT & SGOT reagent kits.  Results: The results showed that the leaves extract of figs (Ficus carica L.) proved to be able to reduce the levels of SGPT & SGOT with the most potent dosage was 40 mg/200 g BW. However, the reduction of SGPT & SGOT levels were not significantly different from other treatments. Conclusions: Figs (Ficus carica L.) leaves extract showed activity as a hepatoprotective agent, based on the reduction of SGPT & SGOT levels in rat after 14 days of treatment.

scholarly journals Ability of Allium cepa L. root tips and Tradescantia pallida var. purpurea in N-nitrosodiethylamine genotoxicity and mutagenicity evaluation

2010 ◽  
Vol 82 (4) ◽  
pp. 925-932 ◽  
Author(s):  
Claudia R. de Rainho ◽  
Andréa Kaezer ◽  
Claudia A.F. Aiub ◽  
Israel Felzenszwalb
Keyword(s):  
Allium Cepa ◽  
Positive Control ◽  
Negative Control ◽  
Nitroso Compounds ◽  
Repair System ◽  
Root Tips ◽  
Allium Cepa L ◽  
Tradescantia Pallida ◽  
Mutation Assay ◽  
Dose Dependent

N-nitroso compounds, such as N-nitrosodiethylamine (NDEA), can be formed by the reaction of secundary amines with nitrosating agents, and are suspected to be involved in tumors in humans. NDEA has been considered a weak carcinogen in genotoxic assays probably due to the inefficient nitrosamine activation system that is used and/or to the efficient repair system. In this work, we evaluated the sensibility of Allium cepa L. root tips and Tradescantia stamen hair mutation assay (Trad-SH) using Tradescantia pallida var. purpurea for NDEA (0.1; 0.5; 5 and 25mM) genotoxicity and mutagenicity induction. Allium cepa L. was treated with different NDEA concentrations for 3h, for 3 consecutive days, including negative control (distilled water) and positive control maleic hydrazide (MH 30mg/mL). After treatment, the roots were hydrolyzed, squashed, and the mitotic index (MI) and cytological abnormalities were scored. The results revealed a cytostatic effect of NDEA (0.5 and 5mM), showing a significant reduction in the MI. Chromosome stickiness suggests a NDEA toxic effect. T. pallida purpurea did not respond to mutagens with a dose-dependent pattern. In conclusion, our study indicates that the root tips of Allium cepa L. have sensibility to detect NDEA genotoxicity, but not for Trad-SH test.

scholarly journals Cytogenotoxic potential of Terminalia actinophylla (Mart.) bark in Allium cepa L.

2020 ◽  
Vol 9 (9) ◽  
pp. e353997322
Author(s):  
Francisca Mairana Silva de Sousa ◽  
José Rafael da Silva Araujo ◽  
Luciana Maria Fortes Castelo Branco Couto ◽  
Regina Maria Silva Sousa ◽  
Maria das Dores Alves Oliveira ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Allium Cepa L ◽  
Phytochemical Profile ◽  
Chromosomal Changes ◽  
Harmful Effects ◽  
Total Average

Terminalia actinophylla (Mart), commonly known as "chapada", is used in folk medicine for the treatment of diarrhea, diabetes and anti-inflammatory. However, studies on its toxicity are still incipient. The present work was carried out with the objective of evaluating the phytochemical profile and the cytogenotoxic effect of the ethanolic extract of the T. actinophylla bark using the Allium cepa L. seed. Seeds of A. cepa were subjected to different extract concentrations (1.25, 2.5, 5 and 10 mg/mL), a negative control (NC - distilled water) as well as trifluralin (PC - positive control), during 24 h of exposure. The phytochemical profile of the extract was obtained to identify the main secondary metabolites. The cytotoxicity (mitotic index) and the genotoxicity (chromosomal alterations) were analyzed by means of the collection of 5,000 meristematic cells. The statistical analysis was carried out using the Kruskal-Wallis test (p<0.05). The phytochemicals present in the extract were tannins, saponins and reducing sugars. Only the lowest concentration (1.25 mg/ml) of T. actinophylla was cytotoxic in comparison with NC. There was a significant increase in the total average of chromosomal changes to lower concentrations (1.25 and 2.5 mg/ml) compared to NC. Probably, phytochemicals in the extract interfere with the cell cycle and cause DNA damage in A. cepa in lower concentrations. However, if bad studies must be carried out on mammals, since the extract with medicinal purpose can have harmful effects on the organism.

scholarly journals Test of Activities of Hepatoprotector of Senggani Leaf Ethanol Extract (Melastoma Malabathricum L.) ON RAT (Rattus Novergicus) That Induced by Paracetamol

Biospecies ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 1-8
Author(s):  
Yustina Yasnidar Laia ◽  
Yoridha Aulia ◽  
Mita Sahara ◽  
Maxwel Simanjuntak Masdalena
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Ethanol Solution ◽  
Control Group ◽  
Group Iii ◽  
Melastoma Malabathricum ◽  
Group I

Senggani leaves (Melastoma Malabathricum L.) are easily available plans that are sometimes used as traditional medicine. This study aimed to determine the hepatotective activity of the ethanol of senggani leaves (Melastoma Malabathricum L.) on rats (Rattus Novergicus) induced by paracetamol with histopatological parameters. Making extracts was carried out by maceration using 96% ethanol solution. The test animals used were 30 rats (Rattus Novergicus) which were divided into 6 groups. Group I without treatment, group II as negative control, group III as positive control, and group IV, V, and VI who were given senggani leaf extract, respectively 100 mg/Kg bb, 200 mg/Kg bb, and 400 mg/Kg bb for 7 day. On the 8 day all rats except group I, were induced paracetamol 180 mg/200 gr bb as the parent of liver demage. The resulst showed that the ethanol extract of senggani leaves at a dose of 400 mg/Kg bb was the most effective in hepatoprotective activity compared to a dose of 100 mg/Kg bb, and 200 mg/Kg bb with histopathological parameters induced by paracetamol.

scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL RIMPANG LENGKUAS (ALPINIA GALANGA) TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (MUS MUSCULUS L.) YANG DIINDUKSI MONOSODIUM GLUTAMATE

2020 ◽  
Vol 7 (2) ◽  
pp. 7-13
Author(s):  
Theodora Agverianti ◽  
Muhartono Muhartono ◽  
Khairun Nisa Berawi
Keyword(s):  
Liver Damage ◽  
Histopathological Examination ◽  
Monosodium Glutamate ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cell Degeneration ◽  
Control Group Design ◽  
Post Test

ABSTRACT Background: Long term consumption of Monosodium Glutamate (MSG) in excess isreported induced liver damage. Toxic effect in liver are due to the degeneration andnecrosis of hepatocytes, which also increases lipid peroxidation. Antioxidant in galangal rhizome is expected to repair liver damage caused by free radicals in MSG. Method: This study is an experimental research with Post Test Only Control Group Design. The samples in this study are 25 mice divided into 5 groups which are, negative control (not given any treatment), positive control (given MSG 4 mg/gBW for 14 days), treatment 1 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 14 mg/20 gBW  for7 days), treatment 2 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 28 mg/20 gBW for 7 days), and treatment 3 (given MSG 4 mg/gBW for 14 days continued with ethanol extract of galangal rhizome 56 mg/20 gBW  for 7 days). Then surgery was needed for histopathological examination. Results: Based on mean scoring results, the results showed calculation of liver cell degeneration for positive control (11,8), negative control (5,2), treatment 1 (10,6), treatment 2 (8,4), and treatment 3 (7,6). Based on the test results One Way ANOVA, the result showed the value of p=0,001 for liver histopathological appearance of mice. Conclusion: There is an effect of ethanol extract of galangal rhizome to the liver histopathological appearance of male mice.

scholarly journals Integrative analysis in toxicological assessment of the insecticide Malathion in Allium cepa L. system

2023 ◽  
Vol 83 ◽  
Author(s):  
N. C. Ghisi ◽  
V. B. Silva ◽  
A. A. Roque ◽  
E. C. Oliveira
Keyword(s):  
Allium Cepa ◽  
Positive Control ◽  
Negative Control ◽  
Integrative Analysis ◽  
Biological Assessment ◽  
Integrative Approach ◽  
Human Populations ◽  
Toxicological Assessment ◽  
L System ◽  
Allium Cepa L

Abstract For many centuries human populations have been suffering and trying to fight with disease-bearing mosquitoes. Emerging and reemerging diseases such as Dengue, Zika, and Chikungunya affect billions of people around the world and recently has been appealing to control with chemical pesticides. Malathion (MT) is one of the main pesticides used against mosquitoes, the vectors of these diseases. This study aimed to assess cytotoxicity and mutagenicity of the malathion for the bioindicator Allium cepa L. using a multivariate and integrative approach. Moreover, an appendix table was compiled with all available literature of insecticides assessed by the Allium cepa system to support our discussion. Exposures during 48h to 0.5 mg mL-1 and 1.0 mg mL-1 MT were compared to the negative control (distilled water) and positive control (MMS solution at 10 mg L-1). The presence of chromosomal aberrations, micronuclei frequency, and mitotic index abnormalities was evaluated. Anaphase bridges were the alterations with higher incidence and presented a significantly elevated rate in the concentration of 0.5 mg mL-1, including when compared to the positive control. The integrative discriminant analysis summarizes that MT in assessed concentrations presented effects like the positive control, corroborating its potential of toxicity to DNA. Therefore, it is concluded that MT in its pure composition and in realistic concentrations used, has genotoxic potential in the biological assessment of A. cepa cells. The multivariate integrative analysis was fundamental to show a whole response of all data, providing a global view of the effect of MT on DNA.

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