Effect of Vitamin D3 on Liver and Kidney Function of Diabetes Mellitus Male Rats

2020 ◽  
pp. 80-83
Keyword(s):  
Kidney Function ◽  
Vitamin D3 ◽  
Total Protein ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group ◽  
Group 4 ◽  
Liver And Kidney ◽  
Group 2 ◽  
Animal House

The present study was carried out in the animal house and laboratories of the College of Veterinary Medicine/ University of Basrah to investigate the role of the administration of vitamin D3 on liver and kidney function of alloxan induced toxic in mature male rats. For this purpose, 48 male rats were divided into 4 groups with 12 animals in each as the following; group (1), animals of were administered with D.W(0.2ml)/day by oral gavages for 6 weeks as control group, group(2) were injected intraperitoneally with 150 mg/kg b.w of alloxan as diabetic group, group (3) were injected with alloxan then administered with 500 UI/kg/day of alpha 25-hydroxy vitamin D3 by gavages for 6 weeks orally, group (4) were injected with alloxan then administer 1000 UI/kg/day of alpha 25-hydroxy vitamin D3 by gavages for 6 weeks orally. At the end of the experiment, animals were euthanized and blood samples were obtained for some biochemical assessment of liver and kidneys.In compares with control, alloxan treated rats recorded a significant increase (p≤0.05)in serum concentration of ALT, AST,ALP ,urea and creatinine, while there was a significant decrease (p≤0.05) in serum total protein. On other hand, administration of vitamin D3 for diabetic rats induced a significant improvement (P≤0.05) of serum ALT, AST, ALP, total protein urea and creatinine.

scholarly journals Evaluation of Kidney Function Parameters in Diabetic Rats Following Virgin Coconut Oil Diet

Folia Medica ◽  
2019 ◽  
Vol 61 (2) ◽  
pp. 249-257 ◽  
Author(s):  
Akinjide M. Akinnuga ◽  
Olubayode Bamidele ◽  
Anthony J. Adewumi
Keyword(s):  
Kidney Function ◽  
Biochemical Analysis ◽  
Coconut Oil ◽  
Diabetic Rats ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Normal Rat ◽  
Group 2 ◽  
Diabetes Control Group ◽  
Group 1

Abstract Background: Diabetes mellitus (DM) leads to disruption of kidney function parameters (KFPs) which are markers of kidney diseases, especially nephropathy. Virgin coconut oil (VCO) has been implicated in playing a significant role in DM management. However, its role on KFPs in DM is scarce. Aim: To evaluate the kidney function parameters following VCO diet in diabetic rats. Materials and methods: : Twenty-five (25) male rats of 150 – 200 g were divided into 5 groups (n=5): Non-diabetic control (Group 1), diabetes control (Group 2), diabetes + metformin (Group 3), diabetes + 10% VCO (Group 4) and diabetes + 20% VCO (Group 5). Apart from Group 1, other groups were given intraperitone-ally 50 mg/kg of streptozotocin to induce diabetes mellitus. After 72 hours, fasting hyperglycaemia was confirmed by glucose oxidase method. All the rats were fed normal rat chow for 8 weeks. At 8th week, serum and urine samples were analysed for biochemical analysis. After 8 weeks, Group 1 and Group 2 continued to be fed on normal rat chow while other groups were treated with diets (VCO) or drug (metformin) for 4 weeks. At 12th week, urine samples were collected for biochemical analysis, the rats were sacrificed, and blood samples were collected by cardiac puncture. Results: There were significant differences in some KFPs in diabetes control (Group 2) compared to other experimental groups. However, there was no significant difference in glomerular filtration rate (GFR) and serum sodium in all the groups. Conclusion: VCO supplementary diet improved the altered KFPs and could be a therapy for kidney problems.

EFFECT OF DIPYRIDAMOLE + ASA ON THE RETINE VASCULAR PATTERN OF ESTREPTOZOTOCIN-DIABETIC RATS

1987 ◽  
Author(s):  
A Moreno ◽  
J P de la Cruz ◽  
J Garcia Campos ◽  
F Sanchez de la Cuesta
Keyword(s):  
Treated Group ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Male Rats ◽  
Control Group ◽  
Vascular Pattern ◽  
Vascular Tree ◽  
Aorta Ring ◽  
Vascular Bed ◽  
Group 2

INTRODUCTIONWe have used an experimental model which allows the evaluation of the qualitative differences in the retinal vascular pattern by means of the labeling of the retine vascular tree with radish peroxidase (HRP) in estreptozotocin-diabetic rats. The aim of the study was to evaluate the effect of ASA and DIP + ASA on the vessels platelet behaviour of said retine pattern in a group of rats in t-hich the diabetes had 3 months of evolution.PROCEDURE22 Wistar male rats were divided into A groups; 1) control group, 2) diabetic rats without antiaggregant, 3) dietetic rats treated with 6 mg/day ASA p.o., 4) diabetic rats treated with 6 mg/day ASA +12 mg/day DIP p.o. For inducing diabetes 30 mg/Kg of i.v. estreptozotocine were administered. The animals were considered “diabetic” when glucemia was over 200 mg/100 ml. After 3 months of treatment with 4IU insuline and ASA, or ASA + DIP, the animals were sacrified. Samples of blood and rings of descending aorta were extracted. Platelet aggregation in IJB in front of 1 μg/ml of collagen and the prostacycline-like activity of the aorta ring were evaluated. The configuration of the retine vascular tree labeled with HRP was observed.RESULTS AND CONCLUSIONSMaximal aggregation intensity: 11.1 Ω in the control group,10.9Ω in the diabetic non-treated group, 4.8Ω in rats receiving ASA and 4.6Ω in rats treated with DIP + ASA. The incUbation during 10 min. of aorta rings in blood samples produced 38.7% inhibition in the control group, 12.8% in the non treated-diabetic group 0% in the ASA group and 49.3% in the group treated with DIP + ASA.The qualitative changes in the diabetic rats retinal vascular network non treated with antiaggregants showed a scarce visibility of capillars as well as large zones of tortuous vessels. The rats treated with ASA showed a continuous vascular bed and less tortuous vessels than the ones in the non treated group but the vascular diameters were smaller than the ones observed in non-diabetic rats; the rats treated with DIP + ASA showed a continuous vascular bed, scarce tortuous vessels and vascular diameters similar to the ones found in non-diabetic rats. Mortality rates: 0% in the control group, 50% in the non-treated diabetic group, 16% in the ASA group and 0% in the DIP + ASA group. The administration of DIP + ASA normalized the prostacycline-like activity and the retinal vascular pattern in estreptozotocin-diabetic rats.

scholarly journals Effect of Aqueous Extract Cyperus rotundus Tubers as Antioxidant on Liver and Kidney Functions in Albino Males Rats Exposed to Cadmium Chloride Toxic

2019 ◽  
Vol 16 (2) ◽  
pp. 0315
Author(s):  
Aldulaimi Et al.
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Cadmium Chloride ◽  
Cyperus Rotundus ◽  
Male Rats ◽  
Oral Dosage ◽  
Control Group ◽  
Protective Effects ◽  
Liver And Kidney ◽  
Kidney Functions

          The experiment was conducted in two stages: first stage: determination of the most effective dose of Cyperus rotundus tubers aquatic extract in male rats for 5 days, which was concentrated as (200 mg/kg body weight). The second stage was designed to observe and test the protective effects of C. rotundus tubers aquatic extract in the liver and kidney functions of male rats exposed to cadmium chloride poisoning (5 mg/kg bw) for 30 days. The animals were divided into (4) groups within each group (5) animals weighted (200-220g). The results of oral dose of cadmium chloride showed a significant increase of (P˂0.05) in the activity of both enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glucose, urea, uric acid, creatinine, malondialdehyde (MDA) and peroxynitrite (ONOO-). And a significant decrease (P˂0.05) in the values ​​of total protein, albumin, globulin and glutathione (GSH) compared with the control group. The oral dosage of the C. rotundus tubers aquatic extract resulted in a significant increase in GSH. The values ​​of each were not significantly different ALT, AST, ALP, Glucose, urea, uric acid, creatinine, total protein, albumin, globulin, MDA and peroxy nitrite Compare with control group. The results of the oral dosage with (C. rotundus tubers aquatic extract + cadmium chloride) showed a positive effect on these values ​​compared with the control group and cadmium chloride. It is concluded that the C. rotundus tubers aquatic extract has protective effects and reduces the effects that cadmium chloride can cause in rats liver and kidney functions through its antioxidant activity and removal of free radicals.

Effects of Salvia Miltiorrhiza extract on the regulation of antioxidant enzyme activities in liver and kidney of rats exposed to TCA

2018 ◽  
Author(s):  
Hanan S. Alnahdi ◽  
Kholoud S. Ramadan ◽  
Hoda E. A. Farid ◽  
Najla O. Ayaz
Keyword(s):  
Body Weight ◽  
Mononuclear Cells ◽  
Salvia Miltiorrhiza ◽  
Treated Group ◽  
Protective Role ◽  
Male Rats ◽  
Antioxidant Enzyme Activities ◽  
Group 4 ◽  
Liver And Kidney ◽  
Group 2

The present study aimed to investigate the protective role of Salvia Miltiorrhiza against Trichloroacetic acid (TCA) induced liver and kidney toxicity in rats. Twenty eight adults Wister albino male rats were divided into 4 equal groups. Group 1 served as control, while group 2 received SM extracts at 200 mg/kg body weight; group 3 was treated with 50 mg/kg/day TCA by gavage daily. Group 4 received the extract at 200 mg/kg body weight and 50 mg/kg/day TCA for two months. A significant increase was observed in liver function, kidney function and MDA (malondialdehyde) levels among the TCA administered animals compared to normal control. Daily oral administration of SM normalized most biochemical changes observed among the TCA treated animals. Histopathologically, higher amount of mononuclear cells infiltration, necrotic cells and few fibroblasts were observed in liver and kidney of TCA treated group. The administration of Salvia Miltiorrhiza extract regulates and decreases liver and kidney damage.

scholarly journals Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2and F2αMetabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Hisham Y. Al-Matubsi ◽  
Ghaleb A. Oriquat ◽  
Mahmoud Abu-Samak ◽  
Othman A. Al Hanbali ◽  
Maher D. Salim
Keyword(s):  
Lipoic Acid ◽  
Fetal Outcome ◽  
Diabetic Rats ◽  
Female Rats ◽  
Control Group ◽  
Total Glutathione ◽  
Group 4 ◽  
Streptozotocin Induced Diabetes ◽  
Group 3 ◽  
Group 2

Background.Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers.Methods.Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1;n=9; and group 2;n=7) or pregnant diabetic (group 3;n=10; and group 4;n=8) rats were treated daily with either LA (groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15. On day 15 of gestation, the fetuses, placentas, and membranes were dissected, examined morphologically, and then homogenized, to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2(PGEM) and PGF2α(PGFM) levels. The level of total glutathione was measured in the maternal liver and plasma and in all fetuses.Results.Supplementation of diabetic rats with LA was found to significantly (p<0.05) reduce resorption rates in diabetic rats and led to a significant (p<0.05) increase in liver, plasma, and fetuses total glutathione from LA-TD rats as compared to those from V-TD. Decreased levels of PGEM and elevated levels of PGFM in the fetuses, placentas, and membranes were characteristic of experimental diabetic gestation associated with malformation. The levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p<0.05) higher than those in malformed fetuses from V-TD rats.Conclusions.LA treatment did not completely prevent the occurrence of malformations. Thus, other factors may be involved in the pathogenesis of the diabetes-induced congenital malformations.

scholarly journals Biochemical and histological effects of ethanolic extracts of fruits of Xylopia aethiopica and seeds and leaves of Piper guineense on liver and kidney function in male albino rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chinedu Imo ◽  
Kayode Adebisi Arowora ◽  
Chukwuma Stephen Ezeonu ◽  
Joseph Ikwebe ◽  
Ojochenemi Ejeh Yakubu ◽  
...  
Keyword(s):  
Kidney Function ◽  
Albino Rats ◽  
Piper Guineense ◽  
Group 4 ◽  
Ethanolic Extracts ◽  
Liver And Kidney ◽  
Group 3 ◽  
Group 2 ◽  
Xylopia Aethiopica ◽  
Group 1

Abstract Background The fruits of Xylopia aethiopica and seeds and leaves of Piper guineense are commonly used as spices. Due to their wide use in nutrition and traditional medicine, there is need to examine the biochemical and histological effects of ethanolic extracts of fruits of Xylopia aethiopica and seeds and leaves of Piper guineense on liver and kidney function in male albino rats. The test animals (groups 2, 3 and 4) received 100 mg/kg each of the corresponding extract for 21 days. Group 1 served as normal control and received a placebo of normal saline. The animals were later fasted overnight, sacrificed and their blood collected through cardiac puncture for biochemical indices. Results AST activity decreased significantly (p < 0.05) in rats of group 2 compared to the normal control; ALP decreased significantly (p < 0.05) in animals of group 3; while ALT increased significantly (p < 0.05) in group 4. The concentrations of urea and potassium showed significant decrease (p < 0.05) in animals of group 3. Creatinine increased significantly (p < 0.05) in group 2. Concentrations of total protein, albumin, globulin, sodium and chlorides in the test animals did not show any significant alteration. WBC count increased significantly (p < 0.05) in rats of group 3 and non-significantly (p > 0.05) in groups 2 and 4. PCV increased significantly in rats of group 4 and non-significantly in groups 2 and 3. RBC count and Hb levels increased non-significantly (p > 0.05) in all the test groups compared to group 1. The histoarchitectural states of the liver and kidneys showed no apparent alteration by the different extracts. Conclusion The extracts have no apparent toxic effect on the liver and kidneys of the experimental rats, although fruits’ extract of Xylopia aethiopica showed possibility of exhibiting mild liver toxicity.

Toxicity of iron oxide nanoparticles, silver nanoparticles and their mixture on antioxidant enzymes and free radicals in male rats

2021 ◽  
Vol 1 (2) ◽  
pp. 009-020
Author(s):  
Ahmed Ibrahim Younus ◽  
Mokhtar Ibrahim Yousef ◽  
Maher Abdel-NabiKamel ◽  
Rakhad Alrawi ◽  
Jubran Mohammed Abdulrahman
Keyword(s):  
Antioxidant Enzymes ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Group 4 ◽  
Total Antioxidant ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

In the present study Wistar male rats were used. Rats were divided into 4 equal groups, 10 rats each. Group 1 served as control, group 2 was administered orally with Fe2O3NPs (5 mg/kg BW; >50 nm), group 3 was treated intraperitoneally with AgNPs (50 mg/kg BW; >100 nm) and group 4 was administered with the mixture of Fe2O3NPs with AgNPs. Animals were treated with the doses every day for 79 days. Results showed significant (P < 0.05) decrease in the antioxidant enzymes (GPX, GST, CAT and SOD) and reduced glutathione (GSH) and total antioxidant capacity (TAC), while significant (P < 0.05) increase in thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) in plasma and testes of rats treated with Fe2O3NPs, AgNPs and their combination compared to control group.

scholarly journals The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat

2018 ◽  
Vol 11 (1) ◽  
pp. 2180-2200
Author(s):  
Nema Abdelhameed Mohamed ◽  
Awatef Mohamed Ali ◽  
Doaa Ahmed Ghareeb ◽  
Adham Rashed Mohamed ◽  
Yasmin Mohamed Elmokhtar
Keyword(s):  
Gene Expression ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Albino Rats ◽  
Group 4 ◽  
Ccl4 Treatment ◽  
Group 5 ◽  
Group 2 ◽  
Wistar Albino Rats

This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male Wistar albino rats were divided into five groups; Group 1: Control; Group 2: DENA-CCl4; Group 3: DENA-CCl4+Cisplatin; Group 4: DENA-CCl4+BBR-NPs; Group 5: DENA-CCl4+Cisplatin+BBR-NPs. DENA-CCl4 significantly increase AST, ALT, ALP, LDH, GGT, AFP activities and total bilirubin, while, 5, NT,  total protein and albumin decreased. DENA-CCl4 treatment caused increment in MDA levels and reduction in SOD, CAT, GPx and GSH in liver tissues. Moreover, DENA-CCl4 increase the gene expression of ADAM17 and TNF-α however,  P53 was declined. In addition, DENA-CCl4 caused severe histopathological lesions in the liver tissue. Interestingly, administration of berberine nanoparticles alone or in combination with cisplatin improves the hepatocarcinogenesis induced by DENA-CCl4 on the physiological, biochemical, molecular and histological levels by decreasing oxidative stress and preserving gene expression of ADAM17, TNF-α and P53. The present findings suggest that BBR-NPs with cisplatin might offer a promising strategy for the prevention of liver cancer.

scholarly journals Effect of Aqueous Extract Cyperus rotundus Tubers as Antioxidant on Liver and Kidney Functions in Albino Males Rats Exposed to Cadmium Chloride Toxic

2019 ◽  
Vol 16 (2) ◽  
pp. 0315
Author(s):  
Aldulaimi Et al.
Keyword(s):  
Uric Acid ◽  
Total Protein ◽  
Cadmium Chloride ◽  
Cyperus Rotundus ◽  
Male Rats ◽  
Oral Dosage ◽  
Control Group ◽  
Protective Effects ◽  
Liver And Kidney ◽  
Kidney Functions

          The experiment was conducted in two stages: first stage: determination of the most effective dose of Cyperus rotundus tubers aquatic extract in male rats for 5 days, which was concentrated as (200 mg/kg body weight). The second stage was designed to observe and test the protective effects of C. rotundus tubers aquatic extract in the liver and kidney functions of male rats exposed to cadmium chloride poisoning (5 mg/kg bw) for 30 days. The animals were divided into (4) groups within each group (5) animals weighted (200-220g). The results of oral dose of cadmium chloride showed a significant increase of (P˂0.05) in the activity of both enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glucose, urea, uric acid, creatinine, malondialdehyde (MDA) and peroxynitrite (ONOO-). And a significant decrease (P˂0.05) in the values ​​of total protein, albumin, globulin and glutathione (GSH) compared with the control group. The oral dosage of the C. rotundus tubers aquatic extract resulted in a significant increase in GSH. The values ​​of each were not significantly different ALT, AST, ALP, Glucose, urea, uric acid, creatinine, total protein, albumin, globulin, MDA and peroxy nitrite Compare with control group. The results of the oral dosage with (C. rotundus tubers aquatic extract + cadmium chloride) showed a positive effect on these values ​​compared with the control group and cadmium chloride. It is concluded that the C. rotundus tubers aquatic extract has protective effects and reduces the effects that cadmium chloride can cause in rats liver and kidney functions through its antioxidant activity and removal of free radicals.

scholarly journals EVALUATION OF TOXICITY PROFILES OF DIFFERENT BRANDS OF PIROXICAM IN WISTAR RATS

2021 ◽  
Vol 9 (6) ◽  
pp. 36-44
Author(s):  
B. Cletus Lemii ◽  
A. Georgewill Onwunari
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Male Rats ◽  
Control Group ◽  
Treatment Groups ◽  
Clinical Dose ◽  
Port Harcourt ◽  
Liver And Kidney ◽  
Animal House ◽  
Department Faculty

BACKGROUD: , a non-steroidal anti-inflammatory drug (NSAID), has been identified to be one of such drugs with great potentials for abuse, addiction and death as well as complaints on the significant dissimilarities and variations of possible organ toxicities across different brands. This study aimed at evaluating toxicity profiles of different brands of in male rats. Healthy thirty (30) male rats, weighing between 180 and 230grams were procured from the animal house of the Pharmacology department, Faculty of Basic Clinical Sciences in University of Port Harcourt, Nigeria. METHODS: The animals were divided into six (6) groups of five (5) animals each. Group1-control group while group2 to 6 were treatment groups. Thereafter, the clinical dose (0.285mg/kg) of brands (A Brand, B Brand, C Brand, D Brand and E Brand) were administered on treatment groups. The analysis was done at the end of the experiment. RESULT: Reveals that, four out of the fives brands demonstrated significant (p&lt;0.05) toxicities on liver and kidney. Two of the brands has predilection for liver toxicity while another two exhibited kidney toxicities. The heart organ was spared from toxic effects of all the five brand understudied. CONCLUSION: The degree of organ toxicities with different brands of varies, however, certain brands tend to have toxicity predilection for either the liver or kidney.

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