Uji Aktivitas Antinosiseptif Kombinasi Ekstrak Daun Dandang Gendis [Clinacanthus nutans (Burn F) Lindau] Dan Daun Bakung (Crinum asiaticum L.) secara In Vivo
The development of new and effective pain medications that are natural isexpected to have the benefits of treatment. The purpose of this study was todetermine the antinociceptive effectiveness of a combination of 75% ethanolextract from dandang gendis leaves and 96% ethanol fraction of bakungleaf. In this study was used chemical method induction (Sigmund's method)in 25 male mice which were divided into 5 treatment groups, namelytreatment group I as negative control was given CMC 0.5%, treatmentgroup II as positive control was given acetosal 100 mg / kg BW, treatmentgroups III, IV and V were given combinations extract. Each group was giventreatment orally, thirty minutes then induced with 0.6% acetic acidintraperitonially, after which it was observed and counted for the amount ofstretching for sixty minutes. Data were analyzed by one-way ANOVA test.The results of this study showed that all treatment groups had a significantdifference (p <0.05), the combination of 75% ethanol extract dandanggendis leaf and 96% ethanol fraction of bakung leaf with a dose of 100 mg/ kg BW had good effectiveness with a percent effectiveness value of 92.50%.The results of this study indicate that the combination of 75% ethanolextract from dandang gendis leaf (Clinacanthus nutans (Burn F) Lindau)and 96% ethanol fraction from bakung leaf (Crinum asiaticum) with thesame concentration ratio, have relatively higher antinociceptive of theactivity of each extract, in other words both of them work together.